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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-181712413-C-CGG (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=181712413&ref=C&alt=CGG&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 181712413,
      "ref": "C",
      "alt": "CGG",
      "effect": "frameshift_variant",
      "transcript": "NM_003106.4",
      "consequences": [
        {
          "aa_ref": "G",
          "aa_alt": "G?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 1,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SOX2",
          "gene_hgnc_id": 11195,
          "hgvs_c": "c.58_59dupGG",
          "hgvs_p": "p.Gly21fs",
          "transcript": "NM_003106.4",
          "protein_id": "NP_003097.1",
          "transcript_support_level": null,
          "aa_start": 20,
          "aa_end": null,
          "aa_length": 317,
          "cds_start": 60,
          "cds_end": null,
          "cds_length": 954,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000325404.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_003106.4"
        },
        {
          "aa_ref": "G",
          "aa_alt": "G?",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 1,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SOX2",
          "gene_hgnc_id": 11195,
          "hgvs_c": "c.58_59dupGG",
          "hgvs_p": "p.Gly21fs",
          "transcript": "ENST00000325404.3",
          "protein_id": "ENSP00000323588.1",
          "transcript_support_level": 6,
          "aa_start": 20,
          "aa_end": null,
          "aa_length": 317,
          "cds_start": 60,
          "cds_end": null,
          "cds_length": 954,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_003106.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000325404.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "SOX2-OT",
          "gene_hgnc_id": 20209,
          "hgvs_c": "n.349+12535_349+12536dupGG",
          "hgvs_p": null,
          "transcript": "ENST00000466034.7",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000466034.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "SOX2-OT",
          "gene_hgnc_id": 20209,
          "hgvs_c": "n.482-27151_482-27150dupGG",
          "hgvs_p": null,
          "transcript": "ENST00000476964.6",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000476964.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "SOX2-OT",
          "gene_hgnc_id": 20209,
          "hgvs_c": "n.593+12535_593+12536dupGG",
          "hgvs_p": null,
          "transcript": "ENST00000491282.6",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000491282.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "SOX2-OT",
          "gene_hgnc_id": 20209,
          "hgvs_c": "n.541-2767_541-2766dupGG",
          "hgvs_p": null,
          "transcript": "ENST00000498731.6",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000498731.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 5,
          "intron_rank_end": null,
          "gene_symbol": "SOX2-OT",
          "gene_hgnc_id": 20209,
          "hgvs_c": "n.708+12535_708+12536dupGG",
          "hgvs_p": null,
          "transcript": "ENST00000460739.6",
          "protein_id": null,
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000460739.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "SOX2-OT",
          "gene_hgnc_id": 20209,
          "hgvs_c": "n.473-27151_473-27150dupGG",
          "hgvs_p": null,
          "transcript": "ENST00000469278.5",
          "protein_id": null,
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000469278.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "SOX2-OT",
          "gene_hgnc_id": 20209,
          "hgvs_c": "n.391+12535_391+12536dupGG",
          "hgvs_p": null,
          "transcript": "ENST00000477928.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000477928.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "SOX2-OT",
          "gene_hgnc_id": 20209,
          "hgvs_c": "n.309-27151_309-27150dupGG",
          "hgvs_p": null,
          "transcript": "ENST00000485035.4",
          "protein_id": null,
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000485035.4"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "SOX2-OT",
          "gene_hgnc_id": 20209,
          "hgvs_c": "n.355-2767_355-2766dupGG",
          "hgvs_p": null,
          "transcript": "ENST00000492337.6",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000492337.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": 6,
          "intron_rank_end": null,
          "gene_symbol": "SOX2-OT",
          "gene_hgnc_id": 20209,
          "hgvs_c": "n.782+12535_782+12536dupGG",
          "hgvs_p": null,
          "transcript": "ENST00000493521.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
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          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
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        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": null,
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          "intron_rank": 2,
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          "gene_symbol": "SOX2-OT",
          "gene_hgnc_id": 20209,
          "hgvs_c": "n.289-78255_289-78254dupGG",
          "hgvs_p": null,
          "transcript": "ENST00000498226.6",
          "protein_id": null,
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          "aa_start": null,
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          "aa_length": null,
          "cds_start": null,
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          "cdna_start": null,
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        },
        {
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": null,
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          "exon_count": 3,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "SOX2-OT",
          "gene_hgnc_id": 20209,
          "hgvs_c": "n.355+12535_355+12536dupGG",
          "hgvs_p": null,
          "transcript": "ENST00000593330.2",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": null,
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          "biotype": "pseudogene",
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        },
        {
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          "canonical": false,
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          ],
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          "gene_symbol": "SOX2-OT",
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          "transcript": "ENST00000593549.6",
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          "cds_start": null,
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          "biotype": "pseudogene",
          "feature": "ENST00000593549.6"
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": null,
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          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "SOX2-OT",
          "gene_hgnc_id": 20209,
          "hgvs_c": "n.286+12535_286+12536dupGG",
          "hgvs_p": null,
          "transcript": "ENST00000595084.3",
          "protein_id": null,
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          "aa_start": null,
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        },
        {
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          "canonical": false,
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          "strand": true,
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          ],
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          "exon_count": 3,
          "intron_rank": 1,
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          "gene_symbol": "SOX2-OT",
          "gene_hgnc_id": 20209,
          "hgvs_c": "n.286+12535_286+12536dupGG",
          "hgvs_p": null,
          "transcript": "ENST00000595287.1",
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "pseudogene",
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        },
        {
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          ],
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          "gene_symbol": "SOX2-OT",
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        },
        {
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          ],
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          "gene_symbol": "SOX2-OT",
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          "biotype": "pseudogene",
          "feature": "ENST00000597651.6"
        },
        {
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          "canonical": false,
          "protein_coding": false,
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          "consequences": [
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          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "SOX2-OT",
          "gene_hgnc_id": 20209,
          "hgvs_c": "n.638-2767_638-2766dupGG",
          "hgvs_p": null,
          "transcript": "ENST00000597828.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000597828.5"
        },
        {
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          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
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      "splice_score_selected": null,
      "splice_prediction_selected": null,
      "splice_source_selected": null,
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": null,
      "bayesdelnoaf_prediction": null,
      "phylop100way_score": 1.307,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": null,
      "spliceai_max_prediction": null,
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 11,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PVS1,PM2,PP5",
      "acmg_by_gene": [
        {
          "score": 11,
          "benign_score": 0,
          "pathogenic_score": 11,
          "criteria": [
            "PVS1",
            "PM2",
            "PP5"
          ],
          "verdict": "Pathogenic",
          "transcript": "NM_003106.4",
          "gene_symbol": "SOX2",
          "hgnc_id": 11195,
          "effects": [
            "frameshift_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.58_59dupGG",
          "hgvs_p": "p.Gly21fs"
        },
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
            "PM2",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000466034.7",
          "gene_symbol": "SOX2-OT",
          "hgnc_id": 20209,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.349+12535_349+12536dupGG",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Anophthalmia/microphthalmia-esophageal atresia syndrome",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "Anophthalmia/microphthalmia-esophageal atresia syndrome",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}
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