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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-183650535-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=183650535&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 183650535,
      "ref": "G",
      "alt": "A",
      "effect": "5_prime_UTR_premature_start_codon_gain_variant",
      "transcript": "NM_001349428.1",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KLHL24",
          "gene_hgnc_id": 25947,
          "hgvs_c": "c.179G>A",
          "hgvs_p": "p.Arg60His",
          "transcript": "NM_017644.3",
          "protein_id": "NP_060114.2",
          "transcript_support_level": null,
          "aa_start": 60,
          "aa_end": null,
          "aa_length": 600,
          "cds_start": 179,
          "cds_end": null,
          "cds_length": 1803,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000242810.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_017644.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KLHL24",
          "gene_hgnc_id": 25947,
          "hgvs_c": "c.179G>A",
          "hgvs_p": "p.Arg60His",
          "transcript": "ENST00000242810.11",
          "protein_id": "ENSP00000242810.6",
          "transcript_support_level": 1,
          "aa_start": 60,
          "aa_end": null,
          "aa_length": 600,
          "cds_start": 179,
          "cds_end": null,
          "cds_length": 1803,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_017644.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000242810.11"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KLHL24",
          "gene_hgnc_id": 25947,
          "hgvs_c": "c.179G>A",
          "hgvs_p": "p.Arg60His",
          "transcript": "ENST00000454652.6",
          "protein_id": "ENSP00000395012.1",
          "transcript_support_level": 1,
          "aa_start": 60,
          "aa_end": null,
          "aa_length": 600,
          "cds_start": 179,
          "cds_end": null,
          "cds_length": 1803,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000454652.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_premature_start_codon_gain_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KLHL24",
          "gene_hgnc_id": 25947,
          "hgvs_c": "c.-788G>A",
          "hgvs_p": null,
          "transcript": "NM_001349428.1",
          "protein_id": "NP_001336357.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 319,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 960,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349428.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_premature_start_codon_gain_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KLHL24",
          "gene_hgnc_id": 25947,
          "hgvs_c": "c.-788G>A",
          "hgvs_p": null,
          "transcript": "NM_001349429.1",
          "protein_id": "NP_001336358.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 319,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 960,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349429.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KLHL24",
          "gene_hgnc_id": 25947,
          "hgvs_c": "c.179G>A",
          "hgvs_p": "p.Arg60His",
          "transcript": "ENST00000943871.1",
          "protein_id": "ENSP00000613930.1",
          "transcript_support_level": null,
          "aa_start": 60,
          "aa_end": null,
          "aa_length": 652,
          "cds_start": 179,
          "cds_end": null,
          "cds_length": 1959,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000943871.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KLHL24",
          "gene_hgnc_id": 25947,
          "hgvs_c": "c.179G>A",
          "hgvs_p": "p.Arg60His",
          "transcript": "NM_001349413.1",
          "protein_id": "NP_001336342.1",
          "transcript_support_level": null,
          "aa_start": 60,
          "aa_end": null,
          "aa_length": 625,
          "cds_start": 179,
          "cds_end": null,
          "cds_length": 1878,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349413.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KLHL24",
          "gene_hgnc_id": 25947,
          "hgvs_c": "c.179G>A",
          "hgvs_p": "p.Arg60His",
          "transcript": "NM_001349414.1",
          "protein_id": "NP_001336343.1",
          "transcript_support_level": null,
          "aa_start": 60,
          "aa_end": null,
          "aa_length": 625,
          "cds_start": 179,
          "cds_end": null,
          "cds_length": 1878,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349414.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KLHL24",
          "gene_hgnc_id": 25947,
          "hgvs_c": "c.179G>A",
          "hgvs_p": "p.Arg60His",
          "transcript": "NM_001349415.1",
          "protein_id": "NP_001336344.1",
          "transcript_support_level": null,
          "aa_start": 60,
          "aa_end": null,
          "aa_length": 625,
          "cds_start": 179,
          "cds_end": null,
          "cds_length": 1878,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349415.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KLHL24",
          "gene_hgnc_id": 25947,
          "hgvs_c": "c.179G>A",
          "hgvs_p": "p.Arg60His",
          "transcript": "NM_001349416.1",
          "protein_id": "NP_001336345.1",
          "transcript_support_level": null,
          "aa_start": 60,
          "aa_end": null,
          "aa_length": 625,
          "cds_start": 179,
          "cds_end": null,
          "cds_length": 1878,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349416.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KLHL24",
          "gene_hgnc_id": 25947,
          "hgvs_c": "c.179G>A",
          "hgvs_p": "p.Arg60His",
          "transcript": "NM_001349417.1",
          "protein_id": "NP_001336346.1",
          "transcript_support_level": null,
          "aa_start": 60,
          "aa_end": null,
          "aa_length": 625,
          "cds_start": 179,
          "cds_end": null,
          "cds_length": 1878,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349417.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KLHL24",
          "gene_hgnc_id": 25947,
          "hgvs_c": "c.179G>A",
          "hgvs_p": "p.Arg60His",
          "transcript": "NM_001349418.1",
          "protein_id": "NP_001336347.1",
          "transcript_support_level": null,
          "aa_start": 60,
          "aa_end": null,
          "aa_length": 625,
          "cds_start": 179,
          "cds_end": null,
          "cds_length": 1878,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349418.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KLHL24",
          "gene_hgnc_id": 25947,
          "hgvs_c": "c.179G>A",
          "hgvs_p": "p.Arg60His",
          "transcript": "ENST00000853856.1",
          "protein_id": "ENSP00000523915.1",
          "transcript_support_level": null,
          "aa_start": 60,
          "aa_end": null,
          "aa_length": 625,
          "cds_start": 179,
          "cds_end": null,
          "cds_length": 1878,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000853856.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KLHL24",
          "gene_hgnc_id": 25947,
          "hgvs_c": "c.179G>A",
          "hgvs_p": "p.Arg60His",
          "transcript": "ENST00000853858.1",
          "protein_id": "ENSP00000523917.1",
          "transcript_support_level": null,
          "aa_start": 60,
          "aa_end": null,
          "aa_length": 625,
          "cds_start": 179,
          "cds_end": null,
          "cds_length": 1878,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000853858.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KLHL24",
          "gene_hgnc_id": 25947,
          "hgvs_c": "c.179G>A",
          "hgvs_p": "p.Arg60His",
          "transcript": "ENST00000853866.1",
          "protein_id": "ENSP00000523925.1",
          "transcript_support_level": null,
          "aa_start": 60,
          "aa_end": null,
          "aa_length": 625,
          "cds_start": 179,
          "cds_end": null,
          "cds_length": 1878,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000853866.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KLHL24",
          "gene_hgnc_id": 25947,
          "hgvs_c": "c.179G>A",
          "hgvs_p": "p.Arg60His",
          "transcript": "ENST00000853875.1",
          "protein_id": "ENSP00000523934.1",
          "transcript_support_level": null,
          "aa_start": 60,
          "aa_end": null,
          "aa_length": 625,
          "cds_start": 179,
          "cds_end": null,
          "cds_length": 1878,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000853875.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KLHL24",
          "gene_hgnc_id": 25947,
          "hgvs_c": "c.179G>A",
          "hgvs_p": "p.Arg60His",
          "transcript": "ENST00000943850.1",
          "protein_id": "ENSP00000613909.1",
          "transcript_support_level": null,
          "aa_start": 60,
          "aa_end": null,
          "aa_length": 625,
          "cds_start": 179,
          "cds_end": null,
          "cds_length": 1878,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000943850.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KLHL24",
          "gene_hgnc_id": 25947,
          "hgvs_c": "c.179G>A",
          "hgvs_p": "p.Arg60His",
          "transcript": "ENST00000943851.1",
          "protein_id": "ENSP00000613910.1",
          "transcript_support_level": null,
          "aa_start": 60,
          "aa_end": null,
          "aa_length": 625,
          "cds_start": 179,
          "cds_end": null,
          "cds_length": 1878,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000943851.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KLHL24",
          "gene_hgnc_id": 25947,
          "hgvs_c": "c.179G>A",
          "hgvs_p": "p.Arg60His",
          "transcript": "ENST00000943856.1",
          "protein_id": "ENSP00000613915.1",
          "transcript_support_level": null,
          "aa_start": 60,
          "aa_end": null,
          "aa_length": 625,
          "cds_start": 179,
          "cds_end": null,
          "cds_length": 1878,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000943856.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KLHL24",
          "gene_hgnc_id": 25947,
          "hgvs_c": "c.179G>A",
          "hgvs_p": "p.Arg60His",
          "transcript": "ENST00000943873.1",
          "protein_id": "ENSP00000613932.1",
          "transcript_support_level": null,
          "aa_start": 60,
          "aa_end": null,
          "aa_length": 625,
          "cds_start": 179,
          "cds_end": null,
          "cds_length": 1878,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000943873.1"
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        {
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        {
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          "transcript": "ENST00000475827.5",
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          "biotype": "pseudogene",
          "feature": "ENST00000475827.5"
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      ],
      "gene_symbol": "KLHL24",
      "gene_hgnc_id": 25947,
      "dbsnp": "rs1261493748",
      "frequency_reference_population": 0.0000013681332,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 2,
      "gnomad_exomes_af": 0.00000136813,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 2,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9689545631408691,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.872,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9215,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.27,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 9.602,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 6,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM2,PP3_Strong",
      "acmg_by_gene": [
        {
          "score": 6,
          "benign_score": 0,
          "pathogenic_score": 6,
          "criteria": [
            "PM2",
            "PP3_Strong"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NM_001349428.1",
          "gene_symbol": "KLHL24",
          "hgnc_id": 25947,
          "effects": [
            "5_prime_UTR_premature_start_codon_gain_variant"
          ],
          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.-788G>A",
          "hgvs_p": null
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      ],
      "clinvar_disease": "Inborn genetic diseases",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "Inborn genetic diseases",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}