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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-186785899-T-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=186785899&ref=T&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 186785899,
      "ref": "T",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_001967.4",
      "consequences": [
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF4A2",
          "gene_hgnc_id": 3284,
          "hgvs_c": "c.365T>G",
          "hgvs_p": "p.Leu122Arg",
          "transcript": "NM_001967.4",
          "protein_id": "NP_001958.2",
          "transcript_support_level": null,
          "aa_start": 122,
          "aa_end": null,
          "aa_length": 407,
          "cds_start": 365,
          "cds_end": null,
          "cds_length": 1224,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000323963.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001967.4"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF4A2",
          "gene_hgnc_id": 3284,
          "hgvs_c": "c.365T>G",
          "hgvs_p": "p.Leu122Arg",
          "transcript": "ENST00000323963.10",
          "protein_id": "ENSP00000326381.5",
          "transcript_support_level": 1,
          "aa_start": 122,
          "aa_end": null,
          "aa_length": 407,
          "cds_start": 365,
          "cds_end": null,
          "cds_length": 1224,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001967.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000323963.10"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF4A2",
          "gene_hgnc_id": 3284,
          "hgvs_c": "c.368T>G",
          "hgvs_p": "p.Leu123Arg",
          "transcript": "ENST00000440191.6",
          "protein_id": "ENSP00000398370.2",
          "transcript_support_level": 1,
          "aa_start": 123,
          "aa_end": null,
          "aa_length": 408,
          "cds_start": 368,
          "cds_end": null,
          "cds_length": 1227,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000440191.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF4A2",
          "gene_hgnc_id": 3284,
          "hgvs_c": "n.225T>G",
          "hgvs_p": null,
          "transcript": "ENST00000426808.5",
          "protein_id": "ENSP00000392686.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000426808.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF4A2",
          "gene_hgnc_id": 3284,
          "hgvs_c": "n.228T>G",
          "hgvs_p": null,
          "transcript": "ENST00000443963.5",
          "protein_id": "ENSP00000402313.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000443963.5"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF4A2",
          "gene_hgnc_id": 3284,
          "hgvs_c": "c.368T>G",
          "hgvs_p": "p.Leu123Arg",
          "transcript": "ENST00000865630.1",
          "protein_id": "ENSP00000535689.1",
          "transcript_support_level": null,
          "aa_start": 123,
          "aa_end": null,
          "aa_length": 405,
          "cds_start": 368,
          "cds_end": null,
          "cds_length": 1218,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000865630.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF4A2",
          "gene_hgnc_id": 3284,
          "hgvs_c": "c.368T>G",
          "hgvs_p": "p.Leu123Arg",
          "transcript": "ENST00000957080.1",
          "protein_id": "ENSP00000627139.1",
          "transcript_support_level": null,
          "aa_start": 123,
          "aa_end": null,
          "aa_length": 405,
          "cds_start": 368,
          "cds_end": null,
          "cds_length": 1218,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000957080.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF4A2",
          "gene_hgnc_id": 3284,
          "hgvs_c": "c.368T>G",
          "hgvs_p": "p.Leu123Arg",
          "transcript": "ENST00000865627.1",
          "protein_id": "ENSP00000535686.1",
          "transcript_support_level": null,
          "aa_start": 123,
          "aa_end": null,
          "aa_length": 402,
          "cds_start": 368,
          "cds_end": null,
          "cds_length": 1209,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000865627.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF4A2",
          "gene_hgnc_id": 3284,
          "hgvs_c": "c.350T>G",
          "hgvs_p": "p.Leu117Arg",
          "transcript": "ENST00000928252.1",
          "protein_id": "ENSP00000598311.1",
          "transcript_support_level": null,
          "aa_start": 117,
          "aa_end": null,
          "aa_length": 402,
          "cds_start": 350,
          "cds_end": null,
          "cds_length": 1209,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928252.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF4A2",
          "gene_hgnc_id": 3284,
          "hgvs_c": "c.368T>G",
          "hgvs_p": "p.Leu123Arg",
          "transcript": "ENST00000928251.1",
          "protein_id": "ENSP00000598310.1",
          "transcript_support_level": null,
          "aa_start": 123,
          "aa_end": null,
          "aa_length": 401,
          "cds_start": 368,
          "cds_end": null,
          "cds_length": 1206,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928251.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF4A2",
          "gene_hgnc_id": 3284,
          "hgvs_c": "c.347T>G",
          "hgvs_p": "p.Leu116Arg",
          "transcript": "ENST00000957081.1",
          "protein_id": "ENSP00000627140.1",
          "transcript_support_level": null,
          "aa_start": 116,
          "aa_end": null,
          "aa_length": 401,
          "cds_start": 347,
          "cds_end": null,
          "cds_length": 1206,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000957081.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF4A2",
          "gene_hgnc_id": 3284,
          "hgvs_c": "c.368T>G",
          "hgvs_p": "p.Leu123Arg",
          "transcript": "ENST00000865628.1",
          "protein_id": "ENSP00000535687.1",
          "transcript_support_level": null,
          "aa_start": 123,
          "aa_end": null,
          "aa_length": 378,
          "cds_start": 368,
          "cds_end": null,
          "cds_length": 1137,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000865628.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF4A2",
          "gene_hgnc_id": 3284,
          "hgvs_c": "c.368T>G",
          "hgvs_p": "p.Leu123Arg",
          "transcript": "ENST00000928249.1",
          "protein_id": "ENSP00000598308.1",
          "transcript_support_level": null,
          "aa_start": 123,
          "aa_end": null,
          "aa_length": 360,
          "cds_start": 368,
          "cds_end": null,
          "cds_length": 1083,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928249.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF4A2",
          "gene_hgnc_id": 3284,
          "hgvs_c": "c.62T>G",
          "hgvs_p": "p.Leu21Arg",
          "transcript": "ENST00000957082.1",
          "protein_id": "ENSP00000627141.1",
          "transcript_support_level": null,
          "aa_start": 21,
          "aa_end": null,
          "aa_length": 306,
          "cds_start": 62,
          "cds_end": null,
          "cds_length": 921,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000957082.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF4A2",
          "gene_hgnc_id": 3284,
          "hgvs_c": "c.314T>G",
          "hgvs_p": "p.Leu105Arg",
          "transcript": "ENST00000498746.1",
          "protein_id": "ENSP00000462571.1",
          "transcript_support_level": 2,
          "aa_start": 105,
          "aa_end": null,
          "aa_length": 108,
          "cds_start": 314,
          "cds_end": null,
          "cds_length": 328,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000498746.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "EIF4A2",
          "gene_hgnc_id": 3284,
          "hgvs_c": "c.212-265T>G",
          "hgvs_p": null,
          "transcript": "ENST00000865629.1",
          "protein_id": "ENSP00000535688.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 305,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 918,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000865629.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "EIF4A2",
          "gene_hgnc_id": 3284,
          "hgvs_c": "c.212-265T>G",
          "hgvs_p": null,
          "transcript": "ENST00000928247.1",
          "protein_id": "ENSP00000598306.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 299,
          "cds_start": null,
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          "cds_length": 900,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928247.1"
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "EIF4A2",
          "gene_hgnc_id": 3284,
          "hgvs_c": "c.212-265T>G",
          "hgvs_p": null,
          "transcript": "ENST00000928248.1",
          "protein_id": "ENSP00000598307.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 275,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 828,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928248.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "EIF4A2",
          "gene_hgnc_id": 3284,
          "hgvs_c": "c.30-1622T>G",
          "hgvs_p": null,
          "transcript": "ENST00000928250.1",
          "protein_id": "ENSP00000598309.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 105,
          "cds_start": null,
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          "cds_length": 318,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928250.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF4A2",
          "gene_hgnc_id": 3284,
          "hgvs_c": "n.365T>G",
          "hgvs_p": null,
          "transcript": "ENST00000425053.5",
          "protein_id": "ENSP00000413529.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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        {
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        {
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          "transcript": "ENST00000465267.5",
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          "biotype": "retained_intron",
          "feature": "ENST00000465267.5"
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      ],
      "gene_symbol": "EIF4A2",
      "gene_hgnc_id": 3284,
      "dbsnp": null,
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.3823559582233429,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.322,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.7252,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.17,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 7.558,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP2,BP4",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 1,
          "pathogenic_score": 3,
          "criteria": [
            "PM2",
            "PP2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001967.4",
          "gene_symbol": "EIF4A2",
          "hgnc_id": 3284,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.365T>G",
          "hgvs_p": "p.Leu122Arg"
        }
      ],
      "clinvar_disease": "not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not provided",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}