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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-193692066-AAGTT-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=193692066&ref=AAGTT&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 193692066,
      "ref": "AAGTT",
      "alt": "A",
      "effect": "frameshift_variant",
      "transcript": "ENST00000361510.8",
      "consequences": [
        {
          "aa_ref": "VR",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.2990_2993delTTAG",
          "hgvs_p": "p.Val997fs",
          "transcript": "NM_130837.3",
          "protein_id": "NP_570850.2",
          "transcript_support_level": null,
          "aa_start": 997,
          "aa_end": null,
          "aa_length": 1015,
          "cds_start": 2990,
          "cds_end": null,
          "cds_length": 3048,
          "cdna_start": 3160,
          "cdna_end": null,
          "cdna_length": 6429,
          "mane_select": "ENST00000361510.8",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "VR",
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.2990_2993delTTAG",
          "hgvs_p": "p.Val997fs",
          "transcript": "ENST00000361510.8",
          "protein_id": "ENSP00000355324.2",
          "transcript_support_level": 5,
          "aa_start": 997,
          "aa_end": null,
          "aa_length": 1015,
          "cds_start": 2990,
          "cds_end": null,
          "cds_length": 3048,
          "cdna_start": 3160,
          "cdna_end": null,
          "cdna_length": 6429,
          "mane_select": "NM_130837.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "VR",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.2936_2939delTTAG",
          "hgvs_p": "p.Val979fs",
          "transcript": "ENST00000361908.8",
          "protein_id": "ENSP00000354681.3",
          "transcript_support_level": 1,
          "aa_start": 979,
          "aa_end": null,
          "aa_length": 997,
          "cds_start": 2936,
          "cds_end": null,
          "cds_length": 2994,
          "cdna_start": 3128,
          "cdna_end": null,
          "cdna_length": 6397,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "n.676_679delTTAG",
          "hgvs_p": null,
          "transcript": "ENST00000495261.1",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3660,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "VR",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.2936_2939delTTAG",
          "hgvs_p": "p.Val979fs",
          "transcript": "NM_130836.3",
          "protein_id": "NP_570849.2",
          "transcript_support_level": null,
          "aa_start": 979,
          "aa_end": null,
          "aa_length": 997,
          "cds_start": 2936,
          "cds_end": null,
          "cds_length": 2994,
          "cdna_start": 3106,
          "cdna_end": null,
          "cdna_length": 6375,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "VR",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.2882_2885delTTAG",
          "hgvs_p": "p.Val961fs",
          "transcript": "NM_130835.3",
          "protein_id": "NP_570848.1",
          "transcript_support_level": null,
          "aa_start": 961,
          "aa_end": null,
          "aa_length": 979,
          "cds_start": 2882,
          "cds_end": null,
          "cds_length": 2940,
          "cdna_start": 3052,
          "cdna_end": null,
          "cdna_length": 6321,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "VR",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.2882_2885delTTAG",
          "hgvs_p": "p.Val961fs",
          "transcript": "ENST00000361715.6",
          "protein_id": "ENSP00000355311.2",
          "transcript_support_level": 5,
          "aa_start": 961,
          "aa_end": null,
          "aa_length": 979,
          "cds_start": 2882,
          "cds_end": null,
          "cds_length": 2940,
          "cdna_start": 3116,
          "cdna_end": null,
          "cdna_length": 6384,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "VR",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.2879_2882delTTAG",
          "hgvs_p": "p.Val960fs",
          "transcript": "NM_130834.3",
          "protein_id": "NP_570847.2",
          "transcript_support_level": null,
          "aa_start": 960,
          "aa_end": null,
          "aa_length": 978,
          "cds_start": 2879,
          "cds_end": null,
          "cds_length": 2937,
          "cdna_start": 3049,
          "cdna_end": null,
          "cdna_length": 6318,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "VR",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.2879_2882delTTAG",
          "hgvs_p": "p.Val960fs",
          "transcript": "ENST00000392437.6",
          "protein_id": "ENSP00000376232.2",
          "transcript_support_level": 3,
          "aa_start": 960,
          "aa_end": null,
          "aa_length": 978,
          "cds_start": 2879,
          "cds_end": null,
          "cds_length": 2937,
          "cdna_start": 2977,
          "cdna_end": null,
          "cdna_length": 4087,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "VR",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.2840_2843delTTAG",
          "hgvs_p": "p.Val947fs",
          "transcript": "ENST00000645553.1",
          "protein_id": "ENSP00000494725.1",
          "transcript_support_level": null,
          "aa_start": 947,
          "aa_end": null,
          "aa_length": 965,
          "cds_start": 2840,
          "cds_end": null,
          "cds_length": 2898,
          "cdna_start": 2931,
          "cdna_end": null,
          "cdna_length": 5008,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "VR",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.2828_2831delTTAG",
          "hgvs_p": "p.Val943fs",
          "transcript": "NM_130833.3",
          "protein_id": "NP_570846.1",
          "transcript_support_level": null,
          "aa_start": 943,
          "aa_end": null,
          "aa_length": 961,
          "cds_start": 2828,
          "cds_end": null,
          "cds_length": 2886,
          "cdna_start": 2998,
          "cdna_end": null,
          "cdna_length": 6267,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "VR",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.2828_2831delTTAG",
          "hgvs_p": "p.Val943fs",
          "transcript": "ENST00000361150.6",
          "protein_id": "ENSP00000354781.2",
          "transcript_support_level": 5,
          "aa_start": 943,
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          "aa_length": 961,
          "cds_start": 2828,
          "cds_end": null,
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          "cdna_start": 3062,
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          "cdna_length": 6330,
          "mane_select": null,
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          "biotype": null,
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        },
        {
          "aa_ref": "VR",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 28,
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          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.2825_2828delTTAG",
          "hgvs_p": "p.Val942fs",
          "transcript": "NM_015560.3",
          "protein_id": "NP_056375.2",
          "transcript_support_level": null,
          "aa_start": 942,
          "aa_end": null,
          "aa_length": 960,
          "cds_start": 2825,
          "cds_end": null,
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          "cdna_start": 2995,
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          "mane_select": null,
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          "biotype": null,
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        },
        {
          "aa_ref": "VR",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.2825_2828delTTAG",
          "hgvs_p": "p.Val942fs",
          "transcript": "ENST00000361828.7",
          "protein_id": "ENSP00000354429.3",
          "transcript_support_level": 5,
          "aa_start": 942,
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          "aa_length": 960,
          "cds_start": 2825,
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          "cdna_start": 3059,
          "cdna_end": null,
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          "mane_select": null,
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          "feature": null
        },
        {
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          ],
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          "intron_rank": null,
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          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.2771_2774delTTAG",
          "hgvs_p": "p.Val924fs",
          "transcript": "NM_130832.3",
          "protein_id": "NP_570845.1",
          "transcript_support_level": null,
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          "cds_start": 2771,
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          "cdna_start": 2941,
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "VR",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.2717_2720delTTAG",
          "hgvs_p": "p.Val906fs",
          "transcript": "NM_130831.3",
          "protein_id": "NP_570844.1",
          "transcript_support_level": null,
          "aa_start": 906,
          "aa_end": null,
          "aa_length": 924,
          "cds_start": 2717,
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          "cdna_start": 2887,
          "cdna_end": null,
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          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": "VR",
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          "canonical": false,
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          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.2717_2720delTTAG",
          "hgvs_p": "p.Val906fs",
          "transcript": "ENST00000646793.1",
          "protein_id": "ENSP00000494512.1",
          "transcript_support_level": null,
          "aa_start": 906,
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          "aa_length": 924,
          "cds_start": 2717,
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          "mane_select": null,
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        },
        {
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          "canonical": false,
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          "strand": true,
          "consequences": [
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          ],
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          "exon_count": 31,
          "intron_rank": null,
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          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.2507_2510delTTAG",
          "hgvs_p": "p.Val836fs",
          "transcript": "ENST00000643329.1",
          "protein_id": "ENSP00000493673.1",
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        },
        {
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          "canonical": false,
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          "strand": true,
          "consequences": [
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          ],
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          "intron_rank": null,
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          "gene_symbol": "OPA1",
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          "hgvs_c": "c.2456_2459delTTAG",
          "hgvs_p": "p.Val819fs",
          "transcript": "NM_001354663.2",
          "protein_id": "NP_001341592.1",
          "transcript_support_level": null,
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        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.2453_2456delTTAG",
          "hgvs_p": "p.Val818fs",
          "transcript": "NM_001354664.2",
          "protein_id": "NP_001341593.1",
          "transcript_support_level": null,
          "aa_start": 818,
          "aa_end": null,
          "aa_length": 836,
          "cds_start": 2453,
          "cds_end": null,
          "cds_length": 2511,
          "cdna_start": 3053,
          "cdna_end": null,
          "cdna_length": 6322,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "VR",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 19,
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          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4280,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "n.*1426_*1429delTTAG",
          "hgvs_p": null,
          "transcript": "ENST00000646277.1",
          "protein_id": "ENSP00000495289.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5916,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "n.*2253_*2256delTTAG",
          "hgvs_p": null,
          "transcript": "ENST00000646699.1",
          "protein_id": "ENSP00000493913.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3943,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "OPA1",
      "gene_hgnc_id": 8140,
      "dbsnp": "rs879255560",
      "frequency_reference_population": 0.0000014705623,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 2,
      "gnomad_exomes_af": 0.00000147056,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 2,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": null,
      "computational_prediction_selected": null,
      "computational_source_selected": null,
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": null,
      "bayesdelnoaf_prediction": null,
      "phylop100way_score": 7.296,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 18,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PVS1,PM2,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 18,
          "benign_score": 0,
          "pathogenic_score": 18,
          "criteria": [
            "PVS1",
            "PM2",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000361510.8",
          "gene_symbol": "OPA1",
          "hgnc_id": 8140,
          "effects": [
            "frameshift_variant"
          ],
          "inheritance_mode": "AD,AR,SD",
          "hgvs_c": "c.2990_2993delTTAG",
          "hgvs_p": "p.Val997fs"
        }
      ],
      "clinvar_disease": "Autosomal dominant optic atrophy classic form,Optic atrophy,not provided",
      "clinvar_classification": "Pathogenic/Likely pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:5 LP:1",
      "phenotype_combined": "Autosomal dominant optic atrophy classic form|not provided|Optic atrophy",
      "pathogenicity_classification_combined": "Pathogenic/Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}