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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-194604372-G-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=194604372&ref=G&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 194604372,
      "ref": "G",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001166305.2",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM44",
          "gene_hgnc_id": 25120,
          "hgvs_c": "c.1091C>A",
          "hgvs_p": "p.Pro364Gln",
          "transcript": "NM_001011655.3",
          "protein_id": "NP_001011655.1",
          "transcript_support_level": null,
          "aa_start": 364,
          "aa_end": null,
          "aa_length": 428,
          "cds_start": 1091,
          "cds_end": null,
          "cds_length": 1287,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000347147.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001011655.3"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM44",
          "gene_hgnc_id": 25120,
          "hgvs_c": "c.1091C>A",
          "hgvs_p": "p.Pro364Gln",
          "transcript": "ENST00000347147.9",
          "protein_id": "ENSP00000333355.6",
          "transcript_support_level": 1,
          "aa_start": 364,
          "aa_end": null,
          "aa_length": 428,
          "cds_start": 1091,
          "cds_end": null,
          "cds_length": 1287,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001011655.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000347147.9"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM44",
          "gene_hgnc_id": 25120,
          "hgvs_c": "c.1232C>A",
          "hgvs_p": "p.Pro411Gln",
          "transcript": "ENST00000392432.6",
          "protein_id": "ENSP00000376227.2",
          "transcript_support_level": 1,
          "aa_start": 411,
          "aa_end": null,
          "aa_length": 475,
          "cds_start": 1232,
          "cds_end": null,
          "cds_length": 1428,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000392432.6"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM44",
          "gene_hgnc_id": 25120,
          "hgvs_c": "c.1091C>A",
          "hgvs_p": "p.Pro364Gln",
          "transcript": "ENST00000473092.5",
          "protein_id": "ENSP00000418674.1",
          "transcript_support_level": 1,
          "aa_start": 364,
          "aa_end": null,
          "aa_length": 438,
          "cds_start": 1091,
          "cds_end": null,
          "cds_length": 1317,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000473092.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM44",
          "gene_hgnc_id": 25120,
          "hgvs_c": "c.1087C>A",
          "hgvs_p": "p.Arg363Ser",
          "transcript": "ENST00000381975.7",
          "protein_id": "ENSP00000371402.3",
          "transcript_support_level": 1,
          "aa_start": 363,
          "aa_end": null,
          "aa_length": 396,
          "cds_start": 1087,
          "cds_end": null,
          "cds_length": 1191,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000381975.7"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM44",
          "gene_hgnc_id": 25120,
          "hgvs_c": "c.365C>A",
          "hgvs_p": "p.Pro122Gln",
          "transcript": "ENST00000432352.5",
          "protein_id": "ENSP00000409963.1",
          "transcript_support_level": 1,
          "aa_start": 122,
          "aa_end": null,
          "aa_length": 197,
          "cds_start": 365,
          "cds_end": null,
          "cds_length": 594,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000432352.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM44",
          "gene_hgnc_id": 25120,
          "hgvs_c": "c.283C>A",
          "hgvs_p": "p.Arg95Ser",
          "transcript": "ENST00000429560.1",
          "protein_id": "ENSP00000403053.1",
          "transcript_support_level": 1,
          "aa_start": 95,
          "aa_end": null,
          "aa_length": 119,
          "cds_start": 283,
          "cds_end": null,
          "cds_length": 360,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000429560.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM44",
          "gene_hgnc_id": 25120,
          "hgvs_c": "n.855C>A",
          "hgvs_p": null,
          "transcript": "ENST00000477651.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000477651.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM44",
          "gene_hgnc_id": 25120,
          "hgvs_c": "c.1232C>A",
          "hgvs_p": "p.Pro411Gln",
          "transcript": "NM_001166305.2",
          "protein_id": "NP_001159777.1",
          "transcript_support_level": null,
          "aa_start": 411,
          "aa_end": null,
          "aa_length": 475,
          "cds_start": 1232,
          "cds_end": null,
          "cds_length": 1428,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001166305.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM44",
          "gene_hgnc_id": 25120,
          "hgvs_c": "c.1148C>A",
          "hgvs_p": "p.Pro383Gln",
          "transcript": "ENST00000943214.1",
          "protein_id": "ENSP00000613273.1",
          "transcript_support_level": null,
          "aa_start": 383,
          "aa_end": null,
          "aa_length": 458,
          "cds_start": 1148,
          "cds_end": null,
          "cds_length": 1377,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000943214.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM44",
          "gene_hgnc_id": 25120,
          "hgvs_c": "c.1091C>A",
          "hgvs_p": "p.Pro364Gln",
          "transcript": "NM_138399.5",
          "protein_id": "NP_612408.3",
          "transcript_support_level": null,
          "aa_start": 364,
          "aa_end": null,
          "aa_length": 438,
          "cds_start": 1091,
          "cds_end": null,
          "cds_length": 1317,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_138399.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM44",
          "gene_hgnc_id": 25120,
          "hgvs_c": "c.1112C>A",
          "hgvs_p": "p.Pro371Gln",
          "transcript": "ENST00000943212.1",
          "protein_id": "ENSP00000613271.1",
          "transcript_support_level": null,
          "aa_start": 371,
          "aa_end": null,
          "aa_length": 435,
          "cds_start": 1112,
          "cds_end": null,
          "cds_length": 1308,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000943212.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM44",
          "gene_hgnc_id": 25120,
          "hgvs_c": "c.1087C>A",
          "hgvs_p": "p.Arg363Ser",
          "transcript": "NM_001166306.2",
          "protein_id": "NP_001159778.1",
          "transcript_support_level": null,
          "aa_start": 363,
          "aa_end": null,
          "aa_length": 396,
          "cds_start": 1087,
          "cds_end": null,
          "cds_length": 1191,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001166306.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM44",
          "gene_hgnc_id": 25120,
          "hgvs_c": "c.920C>A",
          "hgvs_p": "p.Pro307Gln",
          "transcript": "ENST00000943213.1",
          "protein_id": "ENSP00000613272.1",
          "transcript_support_level": null,
          "aa_start": 307,
          "aa_end": null,
          "aa_length": 382,
          "cds_start": 920,
          "cds_end": null,
          "cds_length": 1149,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000943213.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM44",
          "gene_hgnc_id": 25120,
          "hgvs_c": "c.950C>A",
          "hgvs_p": "p.Pro317Gln",
          "transcript": "ENST00000936589.1",
          "protein_id": "ENSP00000606648.1",
          "transcript_support_level": null,
          "aa_start": 317,
          "aa_end": null,
          "aa_length": 381,
          "cds_start": 950,
          "cds_end": null,
          "cds_length": 1146,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000936589.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM44",
          "gene_hgnc_id": 25120,
          "hgvs_c": "c.893C>A",
          "hgvs_p": "p.Pro298Gln",
          "transcript": "ENST00000943216.1",
          "protein_id": "ENSP00000613275.1",
          "transcript_support_level": null,
          "aa_start": 298,
          "aa_end": null,
          "aa_length": 373,
          "cds_start": 893,
          "cds_end": null,
          "cds_length": 1122,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000943216.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM44",
          "gene_hgnc_id": 25120,
          "hgvs_c": "c.920C>A",
          "hgvs_p": "p.Pro307Gln",
          "transcript": "ENST00000936587.1",
          "protein_id": "ENSP00000606646.1",
          "transcript_support_level": null,
          "aa_start": 307,
          "aa_end": null,
          "aa_length": 371,
          "cds_start": 920,
          "cds_end": null,
          "cds_length": 1116,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000936587.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM44",
          "gene_hgnc_id": 25120,
          "hgvs_c": "c.791C>A",
          "hgvs_p": "p.Pro264Gln",
          "transcript": "ENST00000943215.1",
          "protein_id": "ENSP00000613274.1",
          "transcript_support_level": null,
          "aa_start": 264,
          "aa_end": null,
          "aa_length": 339,
          "cds_start": 791,
          "cds_end": null,
          "cds_length": 1020,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000943215.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM44",
          "gene_hgnc_id": 25120,
          "hgvs_c": "c.791C>A",
          "hgvs_p": "p.Pro264Gln",
          "transcript": "ENST00000870306.1",
          "protein_id": "ENSP00000540365.1",
          "transcript_support_level": null,
          "aa_start": 264,
          "aa_end": null,
          "aa_length": 328,
          "cds_start": 791,
          "cds_end": null,
          "cds_length": 987,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000870306.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM44",
          "gene_hgnc_id": 25120,
          "hgvs_c": "c.686C>A",
          "hgvs_p": "p.Pro229Gln",
          "transcript": "ENST00000936588.1",
          "protein_id": "ENSP00000606647.1",
          "transcript_support_level": null,
          "aa_start": 229,
          "aa_end": null,
          "aa_length": 293,
          "cds_start": 686,
          "cds_end": null,
          "cds_length": 882,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000936588.1"
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        {
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        {
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          "biotype": "nonsense_mediated_decay",
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      ],
      "gene_symbol": "TMEM44",
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      "dbsnp": "rs151243596",
      "frequency_reference_population": 7.1070576e-7,
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      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 7.10706e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.2752670645713806,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.252,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.2807,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.13,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 4.881,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
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      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001166305.2",
          "gene_symbol": "TMEM44",
          "hgnc_id": 25120,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
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        {
          "score": 1,
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          "criteria": [
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            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "XR_001741083.2",
          "gene_symbol": "LOC105374291",
          "hgnc_id": null,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.741G>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}