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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-33864074-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=33864074&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 33864074,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001162429.3",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDCD6IP",
          "gene_hgnc_id": 8766,
          "hgvs_c": "c.2189C>T",
          "hgvs_p": "p.Ser730Leu",
          "transcript": "NM_013374.6",
          "protein_id": "NP_037506.2",
          "transcript_support_level": null,
          "aa_start": 730,
          "aa_end": null,
          "aa_length": 868,
          "cds_start": 2189,
          "cds_end": null,
          "cds_length": 2607,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000307296.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_013374.6"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDCD6IP",
          "gene_hgnc_id": 8766,
          "hgvs_c": "c.2189C>T",
          "hgvs_p": "p.Ser730Leu",
          "transcript": "ENST00000307296.8",
          "protein_id": "ENSP00000307387.3",
          "transcript_support_level": 1,
          "aa_start": 730,
          "aa_end": null,
          "aa_length": 868,
          "cds_start": 2189,
          "cds_end": null,
          "cds_length": 2607,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_013374.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000307296.8"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDCD6IP",
          "gene_hgnc_id": 8766,
          "hgvs_c": "c.2204C>T",
          "hgvs_p": "p.Ser735Leu",
          "transcript": "ENST00000457054.6",
          "protein_id": "ENSP00000411825.2",
          "transcript_support_level": 1,
          "aa_start": 735,
          "aa_end": null,
          "aa_length": 873,
          "cds_start": 2204,
          "cds_end": null,
          "cds_length": 2622,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000457054.6"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDCD6IP",
          "gene_hgnc_id": 8766,
          "hgvs_c": "c.2309C>T",
          "hgvs_p": "p.Ser770Leu",
          "transcript": "ENST00000965906.1",
          "protein_id": "ENSP00000635965.1",
          "transcript_support_level": null,
          "aa_start": 770,
          "aa_end": null,
          "aa_length": 908,
          "cds_start": 2309,
          "cds_end": null,
          "cds_length": 2727,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965906.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDCD6IP",
          "gene_hgnc_id": 8766,
          "hgvs_c": "c.2294C>T",
          "hgvs_p": "p.Ser765Leu",
          "transcript": "ENST00000965908.1",
          "protein_id": "ENSP00000635967.1",
          "transcript_support_level": null,
          "aa_start": 765,
          "aa_end": null,
          "aa_length": 903,
          "cds_start": 2294,
          "cds_end": null,
          "cds_length": 2712,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965908.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDCD6IP",
          "gene_hgnc_id": 8766,
          "hgvs_c": "c.2204C>T",
          "hgvs_p": "p.Ser735Leu",
          "transcript": "NM_001162429.3",
          "protein_id": "NP_001155901.1",
          "transcript_support_level": null,
          "aa_start": 735,
          "aa_end": null,
          "aa_length": 873,
          "cds_start": 2204,
          "cds_end": null,
          "cds_length": 2622,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001162429.3"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDCD6IP",
          "gene_hgnc_id": 8766,
          "hgvs_c": "c.2186C>T",
          "hgvs_p": "p.Ser729Leu",
          "transcript": "ENST00000866273.1",
          "protein_id": "ENSP00000536332.1",
          "transcript_support_level": null,
          "aa_start": 729,
          "aa_end": null,
          "aa_length": 867,
          "cds_start": 2186,
          "cds_end": null,
          "cds_length": 2604,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000866273.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDCD6IP",
          "gene_hgnc_id": 8766,
          "hgvs_c": "c.2177C>T",
          "hgvs_p": "p.Ser726Leu",
          "transcript": "ENST00000965903.1",
          "protein_id": "ENSP00000635962.1",
          "transcript_support_level": null,
          "aa_start": 726,
          "aa_end": null,
          "aa_length": 864,
          "cds_start": 2177,
          "cds_end": null,
          "cds_length": 2595,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965903.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDCD6IP",
          "gene_hgnc_id": 8766,
          "hgvs_c": "c.2171C>T",
          "hgvs_p": "p.Ser724Leu",
          "transcript": "ENST00000965907.1",
          "protein_id": "ENSP00000635966.1",
          "transcript_support_level": null,
          "aa_start": 724,
          "aa_end": null,
          "aa_length": 862,
          "cds_start": 2171,
          "cds_end": null,
          "cds_length": 2589,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965907.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDCD6IP",
          "gene_hgnc_id": 8766,
          "hgvs_c": "c.2162C>T",
          "hgvs_p": "p.Ser721Leu",
          "transcript": "ENST00000965905.1",
          "protein_id": "ENSP00000635964.1",
          "transcript_support_level": null,
          "aa_start": 721,
          "aa_end": null,
          "aa_length": 859,
          "cds_start": 2162,
          "cds_end": null,
          "cds_length": 2580,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965905.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDCD6IP",
          "gene_hgnc_id": 8766,
          "hgvs_c": "c.2081C>T",
          "hgvs_p": "p.Ser694Leu",
          "transcript": "ENST00000866274.1",
          "protein_id": "ENSP00000536333.1",
          "transcript_support_level": null,
          "aa_start": 694,
          "aa_end": null,
          "aa_length": 832,
          "cds_start": 2081,
          "cds_end": null,
          "cds_length": 2499,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000866274.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDCD6IP",
          "gene_hgnc_id": 8766,
          "hgvs_c": "c.2072C>T",
          "hgvs_p": "p.Ser691Leu",
          "transcript": "ENST00000866275.1",
          "protein_id": "ENSP00000536334.1",
          "transcript_support_level": null,
          "aa_start": 691,
          "aa_end": null,
          "aa_length": 829,
          "cds_start": 2072,
          "cds_end": null,
          "cds_length": 2490,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000866275.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDCD6IP",
          "gene_hgnc_id": 8766,
          "hgvs_c": "c.1940C>T",
          "hgvs_p": "p.Ser647Leu",
          "transcript": "ENST00000965904.1",
          "protein_id": "ENSP00000635963.1",
          "transcript_support_level": null,
          "aa_start": 647,
          "aa_end": null,
          "aa_length": 785,
          "cds_start": 1940,
          "cds_end": null,
          "cds_length": 2358,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965904.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDCD6IP",
          "gene_hgnc_id": 8766,
          "hgvs_c": "c.1634C>T",
          "hgvs_p": "p.Ser545Leu",
          "transcript": "XM_011533252.2",
          "protein_id": "XP_011531554.1",
          "transcript_support_level": null,
          "aa_start": 545,
          "aa_end": null,
          "aa_length": 683,
          "cds_start": 1634,
          "cds_end": null,
          "cds_length": 2052,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011533252.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDCD6IP",
          "gene_hgnc_id": 8766,
          "hgvs_c": "c.1634C>T",
          "hgvs_p": "p.Ser545Leu",
          "transcript": "XM_047447042.1",
          "protein_id": "XP_047302998.1",
          "transcript_support_level": null,
          "aa_start": 545,
          "aa_end": null,
          "aa_length": 683,
          "cds_start": 1634,
          "cds_end": null,
          "cds_length": 2052,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047447042.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDCD6IP",
          "gene_hgnc_id": 8766,
          "hgvs_c": "n.851C>T",
          "hgvs_p": null,
          "transcript": "ENST00000465122.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000465122.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDCD6IP",
          "gene_hgnc_id": 8766,
          "hgvs_c": "n.179C>T",
          "hgvs_p": null,
          "transcript": "ENST00000473593.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000473593.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDCD6IP",
          "gene_hgnc_id": 8766,
          "hgvs_c": "n.712C>T",
          "hgvs_p": null,
          "transcript": "ENST00000489869.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000489869.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDCD6IP",
          "gene_hgnc_id": 8766,
          "hgvs_c": "n.*1419C>T",
          "hgvs_p": null,
          "transcript": "ENST00000648706.1",
          "protein_id": "ENSP00000497537.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000648706.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDCD6IP",
          "gene_hgnc_id": 8766,
          "hgvs_c": "n.*1419C>T",
          "hgvs_p": null,
          "transcript": "ENST00000648706.1",
          "protein_id": "ENSP00000497537.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000648706.1"
        }
      ],
      "gene_symbol": "PDCD6IP",
      "gene_hgnc_id": 8766,
      "dbsnp": "rs1127732",
      "frequency_reference_population": 0.17798358,
      "hom_count_reference_population": 29772,
      "allele_count_reference_population": 287006,
      "gnomad_exomes_af": 0.177422,
      "gnomad_genomes_af": 0.18337,
      "gnomad_exomes_ac": 259103,
      "gnomad_genomes_ac": 27903,
      "gnomad_exomes_homalt": 26818,
      "gnomad_genomes_homalt": 2954,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.0024302303791046143,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.063,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1048,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.53,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.001,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -12,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BA1",
      "acmg_by_gene": [
        {
          "score": -12,
          "benign_score": 12,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "NM_001162429.3",
          "gene_symbol": "PDCD6IP",
          "hgnc_id": 8766,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2204C>T",
          "hgvs_p": "p.Ser735Leu"
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}