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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-38009945-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=38009945&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 38009945,
      "ref": "G",
      "alt": "A",
      "effect": "stop_gained",
      "transcript": "NM_001130964.2",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCD1",
          "gene_hgnc_id": 9060,
          "hgvs_c": "c.1246C>T",
          "hgvs_p": "p.Arg416*",
          "transcript": "NM_006225.4",
          "protein_id": "NP_006216.2",
          "transcript_support_level": null,
          "aa_start": 416,
          "aa_end": null,
          "aa_length": 756,
          "cds_start": 1246,
          "cds_end": null,
          "cds_length": 2271,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000334661.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_006225.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCD1",
          "gene_hgnc_id": 9060,
          "hgvs_c": "c.1246C>T",
          "hgvs_p": "p.Arg416*",
          "transcript": "ENST00000334661.5",
          "protein_id": "ENSP00000335600.4",
          "transcript_support_level": 1,
          "aa_start": 416,
          "aa_end": null,
          "aa_length": 756,
          "cds_start": 1246,
          "cds_end": null,
          "cds_length": 2271,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_006225.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000334661.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCD1",
          "gene_hgnc_id": 9060,
          "hgvs_c": "c.1309C>T",
          "hgvs_p": "p.Arg437*",
          "transcript": "NM_001130964.2",
          "protein_id": "NP_001124436.1",
          "transcript_support_level": null,
          "aa_start": 437,
          "aa_end": null,
          "aa_length": 777,
          "cds_start": 1309,
          "cds_end": null,
          "cds_length": 2334,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001130964.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCD1",
          "gene_hgnc_id": 9060,
          "hgvs_c": "c.1309C>T",
          "hgvs_p": "p.Arg437*",
          "transcript": "ENST00000463876.5",
          "protein_id": "ENSP00000430344.1",
          "transcript_support_level": 2,
          "aa_start": 437,
          "aa_end": null,
          "aa_length": 777,
          "cds_start": 1309,
          "cds_end": null,
          "cds_length": 2334,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000463876.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCD1",
          "gene_hgnc_id": 9060,
          "hgvs_c": "c.1246C>T",
          "hgvs_p": "p.Arg416*",
          "transcript": "ENST00000956065.1",
          "protein_id": "ENSP00000626124.1",
          "transcript_support_level": null,
          "aa_start": 416,
          "aa_end": null,
          "aa_length": 755,
          "cds_start": 1246,
          "cds_end": null,
          "cds_length": 2268,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956065.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCD1",
          "gene_hgnc_id": 9060,
          "hgvs_c": "c.1228C>T",
          "hgvs_p": "p.Arg410*",
          "transcript": "ENST00000909334.1",
          "protein_id": "ENSP00000579393.1",
          "transcript_support_level": null,
          "aa_start": 410,
          "aa_end": null,
          "aa_length": 750,
          "cds_start": 1228,
          "cds_end": null,
          "cds_length": 2253,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909334.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCD1",
          "gene_hgnc_id": 9060,
          "hgvs_c": "c.1246C>T",
          "hgvs_p": "p.Arg416*",
          "transcript": "ENST00000909335.1",
          "protein_id": "ENSP00000579394.1",
          "transcript_support_level": null,
          "aa_start": 416,
          "aa_end": null,
          "aa_length": 742,
          "cds_start": 1246,
          "cds_end": null,
          "cds_length": 2229,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909335.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCD1",
          "gene_hgnc_id": 9060,
          "hgvs_c": "c.1246C>T",
          "hgvs_p": "p.Arg416*",
          "transcript": "ENST00000909332.1",
          "protein_id": "ENSP00000579391.1",
          "transcript_support_level": null,
          "aa_start": 416,
          "aa_end": null,
          "aa_length": 729,
          "cds_start": 1246,
          "cds_end": null,
          "cds_length": 2190,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909332.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCD1",
          "gene_hgnc_id": 9060,
          "hgvs_c": "c.1246C>T",
          "hgvs_p": "p.Arg416*",
          "transcript": "ENST00000937408.1",
          "protein_id": "ENSP00000607467.1",
          "transcript_support_level": null,
          "aa_start": 416,
          "aa_end": null,
          "aa_length": 717,
          "cds_start": 1246,
          "cds_end": null,
          "cds_length": 2154,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000937408.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCD1",
          "gene_hgnc_id": 9060,
          "hgvs_c": "c.1081C>T",
          "hgvs_p": "p.Arg361*",
          "transcript": "ENST00000909336.1",
          "protein_id": "ENSP00000579395.1",
          "transcript_support_level": null,
          "aa_start": 361,
          "aa_end": null,
          "aa_length": 701,
          "cds_start": 1081,
          "cds_end": null,
          "cds_length": 2106,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909336.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": 7,
          "intron_rank_end": null,
          "gene_symbol": "PLCD1",
          "gene_hgnc_id": 9060,
          "hgvs_c": "c.1138-134C>T",
          "hgvs_p": null,
          "transcript": "ENST00000909333.1",
          "protein_id": "ENSP00000579392.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 706,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2121,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909333.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": 7,
          "intron_rank_end": null,
          "gene_symbol": "PLCD1",
          "gene_hgnc_id": 9060,
          "hgvs_c": "c.1138-134C>T",
          "hgvs_p": null,
          "transcript": "ENST00000956064.1",
          "protein_id": "ENSP00000626123.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 679,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2040,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956064.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": 6,
          "intron_rank_end": null,
          "gene_symbol": "PLCD1",
          "gene_hgnc_id": 9060,
          "hgvs_c": "c.955-134C>T",
          "hgvs_p": null,
          "transcript": "ENST00000956063.1",
          "protein_id": "ENSP00000626122.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 645,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1938,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956063.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCD1",
          "gene_hgnc_id": 9060,
          "hgvs_c": "n.1343C>T",
          "hgvs_p": null,
          "transcript": "ENST00000461445.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000461445.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCD1",
          "gene_hgnc_id": 9060,
          "hgvs_c": "n.393C>T",
          "hgvs_p": null,
          "transcript": "ENST00000484829.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000484829.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCD1",
          "gene_hgnc_id": 9060,
          "hgvs_c": "n.1473C>T",
          "hgvs_p": null,
          "transcript": "NR_024071.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_024071.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCD1",
          "gene_hgnc_id": 9060,
          "hgvs_c": "n.-62C>T",
          "hgvs_p": null,
          "transcript": "ENST00000495367.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000495367.1"
        }
      ],
      "gene_symbol": "PLCD1",
      "gene_hgnc_id": 9060,
      "dbsnp": "rs397514470",
      "frequency_reference_population": 0.000016737937,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 27,
      "gnomad_exomes_af": 0.0000164295,
      "gnomad_genomes_af": 0.0000196956,
      "gnomad_exomes_ac": 24,
      "gnomad_genomes_ac": 3,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.28999999165534973,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.29,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 0.939,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 9,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PVS1,PP5",
      "acmg_by_gene": [
        {
          "score": 9,
          "benign_score": 0,
          "pathogenic_score": 9,
          "criteria": [
            "PVS1",
            "PP5"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NM_001130964.2",
          "gene_symbol": "PLCD1",
          "hgnc_id": 9060,
          "effects": [
            "stop_gained"
          ],
          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.1309C>T",
          "hgvs_p": "p.Arg437*"
        }
      ],
      "clinvar_disease": "Nonsyndromic congenital nail disorder 3",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "Nonsyndromic congenital nail disorder 3",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}