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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-42635269-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=42635269&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 42635269,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_001349124.2",
      "consequences": [
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NKTR",
          "gene_hgnc_id": 7833,
          "hgvs_c": "c.1066G>C",
          "hgvs_p": "p.Asp356His",
          "transcript": "NM_005385.4",
          "protein_id": "NP_005376.2",
          "transcript_support_level": null,
          "aa_start": 356,
          "aa_end": null,
          "aa_length": 1462,
          "cds_start": 1066,
          "cds_end": null,
          "cds_length": 4389,
          "cdna_start": 1182,
          "cdna_end": null,
          "cdna_length": 7265,
          "mane_select": "ENST00000232978.13",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_005385.4"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NKTR",
          "gene_hgnc_id": 7833,
          "hgvs_c": "c.1066G>C",
          "hgvs_p": "p.Asp356His",
          "transcript": "ENST00000232978.13",
          "protein_id": "ENSP00000232978.8",
          "transcript_support_level": 1,
          "aa_start": 356,
          "aa_end": null,
          "aa_length": 1462,
          "cds_start": 1066,
          "cds_end": null,
          "cds_length": 4389,
          "cdna_start": 1182,
          "cdna_end": null,
          "cdna_length": 7265,
          "mane_select": "NM_005385.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000232978.13"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NKTR",
          "gene_hgnc_id": 7833,
          "hgvs_c": "c.1066G>C",
          "hgvs_p": "p.Asp356His",
          "transcript": "NM_001349124.2",
          "protein_id": "NP_001336053.1",
          "transcript_support_level": null,
          "aa_start": 356,
          "aa_end": null,
          "aa_length": 1463,
          "cds_start": 1066,
          "cds_end": null,
          "cds_length": 4392,
          "cdna_start": 1182,
          "cdna_end": null,
          "cdna_length": 7268,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349124.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NKTR",
          "gene_hgnc_id": 7833,
          "hgvs_c": "c.1066G>C",
          "hgvs_p": "p.Asp356His",
          "transcript": "ENST00000937553.1",
          "protein_id": "ENSP00000607612.1",
          "transcript_support_level": null,
          "aa_start": 356,
          "aa_end": null,
          "aa_length": 1463,
          "cds_start": 1066,
          "cds_end": null,
          "cds_length": 4392,
          "cdna_start": 1231,
          "cdna_end": null,
          "cdna_length": 7317,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000937553.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NKTR",
          "gene_hgnc_id": 7833,
          "hgvs_c": "c.1066G>C",
          "hgvs_p": "p.Asp356His",
          "transcript": "ENST00000970640.1",
          "protein_id": "ENSP00000640699.1",
          "transcript_support_level": null,
          "aa_start": 356,
          "aa_end": null,
          "aa_length": 1463,
          "cds_start": 1066,
          "cds_end": null,
          "cds_length": 4392,
          "cdna_start": 1243,
          "cdna_end": null,
          "cdna_length": 7330,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000970640.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NKTR",
          "gene_hgnc_id": 7833,
          "hgvs_c": "c.1066G>C",
          "hgvs_p": "p.Asp356His",
          "transcript": "ENST00000937557.1",
          "protein_id": "ENSP00000607616.1",
          "transcript_support_level": null,
          "aa_start": 356,
          "aa_end": null,
          "aa_length": 1462,
          "cds_start": 1066,
          "cds_end": null,
          "cds_length": 4389,
          "cdna_start": 1226,
          "cdna_end": null,
          "cdna_length": 7309,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000937557.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NKTR",
          "gene_hgnc_id": 7833,
          "hgvs_c": "c.1036G>C",
          "hgvs_p": "p.Asp346His",
          "transcript": "ENST00000937556.1",
          "protein_id": "ENSP00000607615.1",
          "transcript_support_level": null,
          "aa_start": 346,
          "aa_end": null,
          "aa_length": 1452,
          "cds_start": 1036,
          "cds_end": null,
          "cds_length": 4359,
          "cdna_start": 1136,
          "cdna_end": null,
          "cdna_length": 7217,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000937556.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NKTR",
          "gene_hgnc_id": 7833,
          "hgvs_c": "c.1033G>C",
          "hgvs_p": "p.Asp345His",
          "transcript": "ENST00000937555.1",
          "protein_id": "ENSP00000607614.1",
          "transcript_support_level": null,
          "aa_start": 345,
          "aa_end": null,
          "aa_length": 1451,
          "cds_start": 1033,
          "cds_end": null,
          "cds_length": 4356,
          "cdna_start": 1148,
          "cdna_end": null,
          "cdna_length": 7234,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000937555.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NKTR",
          "gene_hgnc_id": 7833,
          "hgvs_c": "c.976G>C",
          "hgvs_p": "p.Asp326His",
          "transcript": "ENST00000937554.1",
          "protein_id": "ENSP00000607613.1",
          "transcript_support_level": null,
          "aa_start": 326,
          "aa_end": null,
          "aa_length": 1432,
          "cds_start": 976,
          "cds_end": null,
          "cds_length": 4299,
          "cdna_start": 1132,
          "cdna_end": null,
          "cdna_length": 7215,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000937554.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NKTR",
          "gene_hgnc_id": 7833,
          "hgvs_c": "c.307G>C",
          "hgvs_p": "p.Asp103His",
          "transcript": "NM_001349125.2",
          "protein_id": "NP_001336054.1",
          "transcript_support_level": null,
          "aa_start": 103,
          "aa_end": null,
          "aa_length": 1210,
          "cds_start": 307,
          "cds_end": null,
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          "cdna_start": 1238,
          "cdna_end": null,
          "cdna_length": 7324,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001349125.2"
        },
        {
          "aa_ref": "D",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NKTR",
          "gene_hgnc_id": 7833,
          "hgvs_c": "c.1066G>C",
          "hgvs_p": "p.Asp356His",
          "transcript": "XM_024453539.2",
          "protein_id": "XP_024309307.1",
          "transcript_support_level": null,
          "aa_start": 356,
          "aa_end": null,
          "aa_length": 1463,
          "cds_start": 1066,
          "cds_end": null,
          "cds_length": 4392,
          "cdna_start": 1102,
          "cdna_end": null,
          "cdna_length": 7188,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "D",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NKTR",
          "gene_hgnc_id": 7833,
          "hgvs_c": "c.1066G>C",
          "hgvs_p": "p.Asp356His",
          "transcript": "XM_006713171.3",
          "protein_id": "XP_006713234.1",
          "transcript_support_level": null,
          "aa_start": 356,
          "aa_end": null,
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          "cds_start": 1066,
          "cds_end": null,
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          "cdna_start": 1182,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "D",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
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          "gene_symbol": "NKTR",
          "gene_hgnc_id": 7833,
          "hgvs_c": "c.1066G>C",
          "hgvs_p": "p.Asp356His",
          "transcript": "XM_017006474.3",
          "protein_id": "XP_016861963.1",
          "transcript_support_level": null,
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          "cds_start": 1066,
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          "cdna_start": 1182,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_017006474.3"
        },
        {
          "aa_ref": "D",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NKTR",
          "gene_hgnc_id": 7833,
          "hgvs_c": "c.736G>C",
          "hgvs_p": "p.Asp246His",
          "transcript": "XM_047448192.1",
          "protein_id": "XP_047304148.1",
          "transcript_support_level": null,
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          "cds_start": 736,
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        {
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          "gene_symbol": "NKTR",
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          "hgvs_c": "c.736G>C",
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          "transcript": "XM_047448193.1",
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_047448193.1"
        },
        {
          "aa_ref": "D",
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          "protein_coding": true,
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          "consequences": [
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          ],
          "exon_rank": 9,
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          "exon_count": 14,
          "intron_rank": null,
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          "gene_symbol": "NKTR",
          "gene_hgnc_id": 7833,
          "hgvs_c": "c.736G>C",
          "hgvs_p": "p.Asp246His",
          "transcript": "XM_047448194.1",
          "protein_id": "XP_047304150.1",
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        },
        {
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          ],
          "exon_rank": 7,
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          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NKTR",
          "gene_hgnc_id": 7833,
          "hgvs_c": "c.307G>C",
          "hgvs_p": "p.Asp103His",
          "transcript": "XM_047448195.1",
          "protein_id": "XP_047304151.1",
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        {
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          ],
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          "exon_count": 19,
          "intron_rank": null,
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          "gene_symbol": "NKTR",
          "gene_hgnc_id": 7833,
          "hgvs_c": "c.-39G>C",
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          "transcript": "NM_001349126.2",
          "protein_id": "NP_001336055.1",
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          "cds_start": null,
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        },
        {
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          ],
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          "intron_rank": 9,
          "intron_rank_end": null,
          "gene_symbol": "NKTR",
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          "hgvs_c": "c.773+2446G>C",
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          "transcript": "ENST00000970641.1",
          "protein_id": "ENSP00000640700.1",
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          "biotype": "protein_coding",
          "feature": "ENST00000970641.1"
        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": 9,
          "intron_rank_end": null,
          "gene_symbol": "NKTR",
          "gene_hgnc_id": 7833,
          "hgvs_c": "c.773+2446G>C",
          "hgvs_p": null,
          "transcript": "ENST00000970642.1",
          "protein_id": "ENSP00000640701.1",
          "transcript_support_level": null,
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          "cds_start": null,
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          "mane_select": null,
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          "gene_hgnc_id": 41174,
          "hgvs_c": "n.396-11457C>G",
          "hgvs_p": null,
          "transcript": "NR_125400.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 502,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_125400.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NKTR",
          "gene_hgnc_id": 7833,
          "hgvs_c": "n.*212G>C",
          "hgvs_p": null,
          "transcript": "ENST00000464315.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 544,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000464315.1"
        }
      ],
      "gene_symbol": "NKTR",
      "gene_hgnc_id": 7833,
      "dbsnp": "rs765491779",
      "frequency_reference_population": 0.0000013683915,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 2,
      "gnomad_exomes_af": 0.00000136839,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 2,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.17464634776115417,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.164,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.2843,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.36,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 7.771,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001349124.2",
          "gene_symbol": "NKTR",
          "hgnc_id": 7833,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.1066G>C",
          "hgvs_p": "p.Asp356His"
        },
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000765508.1",
          "gene_symbol": "ZBTB47-AS1",
          "hgnc_id": 41174,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.463C>G",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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