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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-46676294-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=46676294&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 46676294,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_147129.5",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALS2CL",
          "gene_hgnc_id": 20605,
          "hgvs_c": "c.2137G>A",
          "hgvs_p": "p.Ala713Thr",
          "transcript": "NM_147129.5",
          "protein_id": "NP_667340.2",
          "transcript_support_level": null,
          "aa_start": 713,
          "aa_end": null,
          "aa_length": 953,
          "cds_start": 2137,
          "cds_end": null,
          "cds_length": 2862,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000318962.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_147129.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALS2CL",
          "gene_hgnc_id": 20605,
          "hgvs_c": "c.2137G>A",
          "hgvs_p": "p.Ala713Thr",
          "transcript": "ENST00000318962.9",
          "protein_id": "ENSP00000313670.4",
          "transcript_support_level": 1,
          "aa_start": 713,
          "aa_end": null,
          "aa_length": 953,
          "cds_start": 2137,
          "cds_end": null,
          "cds_length": 2862,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_147129.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000318962.9"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALS2CL",
          "gene_hgnc_id": 20605,
          "hgvs_c": "n.*525G>A",
          "hgvs_p": null,
          "transcript": "ENST00000434140.5",
          "protein_id": "ENSP00000405335.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000434140.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALS2CL",
          "gene_hgnc_id": 20605,
          "hgvs_c": "n.*525G>A",
          "hgvs_p": null,
          "transcript": "ENST00000434140.5",
          "protein_id": "ENSP00000405335.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000434140.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALS2CL",
          "gene_hgnc_id": 20605,
          "hgvs_c": "c.2188G>A",
          "hgvs_p": "p.Ala730Thr",
          "transcript": "ENST00000950707.1",
          "protein_id": "ENSP00000620766.1",
          "transcript_support_level": null,
          "aa_start": 730,
          "aa_end": null,
          "aa_length": 981,
          "cds_start": 2188,
          "cds_end": null,
          "cds_length": 2946,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000950707.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALS2CL",
          "gene_hgnc_id": 20605,
          "hgvs_c": "c.2188G>A",
          "hgvs_p": "p.Ala730Thr",
          "transcript": "ENST00000860447.1",
          "protein_id": "ENSP00000530506.1",
          "transcript_support_level": null,
          "aa_start": 730,
          "aa_end": null,
          "aa_length": 970,
          "cds_start": 2188,
          "cds_end": null,
          "cds_length": 2913,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000860447.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALS2CL",
          "gene_hgnc_id": 20605,
          "hgvs_c": "c.2188G>A",
          "hgvs_p": "p.Ala730Thr",
          "transcript": "ENST00000950702.1",
          "protein_id": "ENSP00000620761.1",
          "transcript_support_level": null,
          "aa_start": 730,
          "aa_end": null,
          "aa_length": 970,
          "cds_start": 2188,
          "cds_end": null,
          "cds_length": 2913,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000950702.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALS2CL",
          "gene_hgnc_id": 20605,
          "hgvs_c": "c.2188G>A",
          "hgvs_p": "p.Ala730Thr",
          "transcript": "ENST00000950703.1",
          "protein_id": "ENSP00000620762.1",
          "transcript_support_level": null,
          "aa_start": 730,
          "aa_end": null,
          "aa_length": 970,
          "cds_start": 2188,
          "cds_end": null,
          "cds_length": 2913,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000950703.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALS2CL",
          "gene_hgnc_id": 20605,
          "hgvs_c": "c.2188G>A",
          "hgvs_p": "p.Ala730Thr",
          "transcript": "ENST00000950705.1",
          "protein_id": "ENSP00000620764.1",
          "transcript_support_level": null,
          "aa_start": 730,
          "aa_end": null,
          "aa_length": 970,
          "cds_start": 2188,
          "cds_end": null,
          "cds_length": 2913,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000950705.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALS2CL",
          "gene_hgnc_id": 20605,
          "hgvs_c": "c.2188G>A",
          "hgvs_p": "p.Ala730Thr",
          "transcript": "ENST00000950712.1",
          "protein_id": "ENSP00000620771.1",
          "transcript_support_level": null,
          "aa_start": 730,
          "aa_end": null,
          "aa_length": 970,
          "cds_start": 2188,
          "cds_end": null,
          "cds_length": 2913,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000950712.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALS2CL",
          "gene_hgnc_id": 20605,
          "hgvs_c": "c.2173G>A",
          "hgvs_p": "p.Ala725Thr",
          "transcript": "ENST00000950706.1",
          "protein_id": "ENSP00000620765.1",
          "transcript_support_level": null,
          "aa_start": 725,
          "aa_end": null,
          "aa_length": 965,
          "cds_start": 2173,
          "cds_end": null,
          "cds_length": 2898,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000950706.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALS2CL",
          "gene_hgnc_id": 20605,
          "hgvs_c": "c.2161G>A",
          "hgvs_p": "p.Ala721Thr",
          "transcript": "ENST00000950708.1",
          "protein_id": "ENSP00000620767.1",
          "transcript_support_level": null,
          "aa_start": 721,
          "aa_end": null,
          "aa_length": 961,
          "cds_start": 2161,
          "cds_end": null,
          "cds_length": 2886,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000950708.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALS2CL",
          "gene_hgnc_id": 20605,
          "hgvs_c": "c.2137G>A",
          "hgvs_p": "p.Ala713Thr",
          "transcript": "NM_001190707.2",
          "protein_id": "NP_001177636.1",
          "transcript_support_level": null,
          "aa_start": 713,
          "aa_end": null,
          "aa_length": 953,
          "cds_start": 2137,
          "cds_end": null,
          "cds_length": 2862,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001190707.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALS2CL",
          "gene_hgnc_id": 20605,
          "hgvs_c": "c.2137G>A",
          "hgvs_p": "p.Ala713Thr",
          "transcript": "ENST00000415953.5",
          "protein_id": "ENSP00000413223.1",
          "transcript_support_level": 2,
          "aa_start": 713,
          "aa_end": null,
          "aa_length": 953,
          "cds_start": 2137,
          "cds_end": null,
          "cds_length": 2862,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000415953.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALS2CL",
          "gene_hgnc_id": 20605,
          "hgvs_c": "c.2137G>A",
          "hgvs_p": "p.Ala713Thr",
          "transcript": "ENST00000860443.1",
          "protein_id": "ENSP00000530502.1",
          "transcript_support_level": null,
          "aa_start": 713,
          "aa_end": null,
          "aa_length": 953,
          "cds_start": 2137,
          "cds_end": null,
          "cds_length": 2862,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000860443.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALS2CL",
          "gene_hgnc_id": 20605,
          "hgvs_c": "c.2137G>A",
          "hgvs_p": "p.Ala713Thr",
          "transcript": "ENST00000860444.1",
          "protein_id": "ENSP00000530503.1",
          "transcript_support_level": null,
          "aa_start": 713,
          "aa_end": null,
          "aa_length": 953,
          "cds_start": 2137,
          "cds_end": null,
          "cds_length": 2862,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000860444.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALS2CL",
          "gene_hgnc_id": 20605,
          "hgvs_c": "c.2137G>A",
          "hgvs_p": "p.Ala713Thr",
          "transcript": "ENST00000860445.1",
          "protein_id": "ENSP00000530504.1",
          "transcript_support_level": null,
          "aa_start": 713,
          "aa_end": null,
          "aa_length": 953,
          "cds_start": 2137,
          "cds_end": null,
          "cds_length": 2862,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000860445.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALS2CL",
          "gene_hgnc_id": 20605,
          "hgvs_c": "c.2137G>A",
          "hgvs_p": "p.Ala713Thr",
          "transcript": "ENST00000860450.1",
          "protein_id": "ENSP00000530509.1",
          "transcript_support_level": null,
          "aa_start": 713,
          "aa_end": null,
          "aa_length": 953,
          "cds_start": 2137,
          "cds_end": null,
          "cds_length": 2862,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000860450.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALS2CL",
          "gene_hgnc_id": 20605,
          "hgvs_c": "c.2137G>A",
          "hgvs_p": "p.Ala713Thr",
          "transcript": "ENST00000860452.1",
          "protein_id": "ENSP00000530511.1",
          "transcript_support_level": null,
          "aa_start": 713,
          "aa_end": null,
          "aa_length": 953,
          "cds_start": 2137,
          "cds_end": null,
          "cds_length": 2862,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000860452.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALS2CL",
          "gene_hgnc_id": 20605,
          "hgvs_c": "c.2137G>A",
          "hgvs_p": "p.Ala713Thr",
          "transcript": "ENST00000950701.1",
          "protein_id": "ENSP00000620760.1",
          "transcript_support_level": null,
          "aa_start": 713,
          "aa_end": null,
          "aa_length": 953,
          "cds_start": 2137,
          "cds_end": null,
          "cds_length": 2862,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
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      "gnomad_exomes_af": 0.0000212109,
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      "computational_score_selected": 0.273232102394104,
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      "computational_source_selected": "MetaRNN",
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      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "revel_prediction": "Benign",
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      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.06,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 6.638,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.03,
      "spliceai_max_prediction": "Benign",
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      "mitotip_prediction": null,
      "acmg_score": -5,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4,BS2",
      "acmg_by_gene": [
        {
          "score": -5,
          "benign_score": 5,
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            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_147129.5",
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          "effects": [
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          "inheritance_mode": "AD",
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}