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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-48627947-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=48627947&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 48627947,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant,splice_region_variant",
      "transcript": "ENST00000395550.7",
      "consequences": [
        {
          "aa_ref": "M",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A6",
          "gene_hgnc_id": 14472,
          "hgvs_c": "c.1892T>C",
          "hgvs_p": "p.Met631Thr",
          "transcript": "NM_022911.3",
          "protein_id": "NP_075062.2",
          "transcript_support_level": null,
          "aa_start": 631,
          "aa_end": null,
          "aa_length": 759,
          "cds_start": 1892,
          "cds_end": null,
          "cds_length": 2280,
          "cdna_start": 1960,
          "cdna_end": null,
          "cdna_length": 2611,
          "mane_select": "ENST00000395550.7",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A6",
          "gene_hgnc_id": 14472,
          "hgvs_c": "c.1892T>C",
          "hgvs_p": "p.Met631Thr",
          "transcript": "ENST00000395550.7",
          "protein_id": "ENSP00000378920.2",
          "transcript_support_level": 1,
          "aa_start": 631,
          "aa_end": null,
          "aa_length": 759,
          "cds_start": 1892,
          "cds_end": null,
          "cds_length": 2280,
          "cdna_start": 1960,
          "cdna_end": null,
          "cdna_length": 2611,
          "mane_select": "NM_022911.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A6",
          "gene_hgnc_id": 14472,
          "hgvs_c": "c.1892T>C",
          "hgvs_p": "p.Met631Thr",
          "transcript": "ENST00000420764.6",
          "protein_id": "ENSP00000404684.2",
          "transcript_support_level": 1,
          "aa_start": 631,
          "aa_end": null,
          "aa_length": 758,
          "cds_start": 1892,
          "cds_end": null,
          "cds_length": 2277,
          "cdna_start": 1982,
          "cdna_end": null,
          "cdna_length": 2630,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A6",
          "gene_hgnc_id": 14472,
          "hgvs_c": "c.1829T>C",
          "hgvs_p": "p.Met610Thr",
          "transcript": "ENST00000358747.10",
          "protein_id": "ENSP00000351597.6",
          "transcript_support_level": 1,
          "aa_start": 610,
          "aa_end": null,
          "aa_length": 738,
          "cds_start": 1829,
          "cds_end": null,
          "cds_length": 2217,
          "cdna_start": 2080,
          "cdna_end": null,
          "cdna_length": 2729,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A6",
          "gene_hgnc_id": 14472,
          "hgvs_c": "n.2080T>C",
          "hgvs_p": null,
          "transcript": "ENST00000480524.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2727,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": 16,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A6",
          "gene_hgnc_id": 14472,
          "hgvs_c": "c.1819+468T>C",
          "hgvs_p": null,
          "transcript": "ENST00000383733.7",
          "protein_id": "ENSP00000373239.3",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 740,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2223,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2545,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": 16,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A6",
          "gene_hgnc_id": 14472,
          "hgvs_c": "n.*1497+468T>C",
          "hgvs_p": null,
          "transcript": "ENST00000307364.10",
          "protein_id": "ENSP00000307089.6",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2586,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A6",
          "gene_hgnc_id": 14472,
          "hgvs_c": "c.1892T>C",
          "hgvs_p": "p.Met631Thr",
          "transcript": "NM_134263.3",
          "protein_id": "NP_599025.2",
          "transcript_support_level": null,
          "aa_start": 631,
          "aa_end": null,
          "aa_length": 758,
          "cds_start": 1892,
          "cds_end": null,
          "cds_length": 2277,
          "cdna_start": 1960,
          "cdna_end": null,
          "cdna_length": 2608,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A6",
          "gene_hgnc_id": 14472,
          "hgvs_c": "c.1829T>C",
          "hgvs_p": "p.Met610Thr",
          "transcript": "NM_001040454.1",
          "protein_id": "NP_001035544.1",
          "transcript_support_level": null,
          "aa_start": 610,
          "aa_end": null,
          "aa_length": 738,
          "cds_start": 1829,
          "cds_end": null,
          "cds_length": 2217,
          "cdna_start": 2080,
          "cdna_end": null,
          "cdna_length": 2731,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A6",
          "gene_hgnc_id": 14472,
          "hgvs_c": "c.1784T>C",
          "hgvs_p": "p.Met595Thr",
          "transcript": "NM_001281732.2",
          "protein_id": "NP_001268661.1",
          "transcript_support_level": null,
          "aa_start": 595,
          "aa_end": null,
          "aa_length": 723,
          "cds_start": 1784,
          "cds_end": null,
          "cds_length": 2172,
          "cdna_start": 1852,
          "cdna_end": null,
          "cdna_length": 2503,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A6",
          "gene_hgnc_id": 14472,
          "hgvs_c": "c.1784T>C",
          "hgvs_p": "p.Met595Thr",
          "transcript": "ENST00000455886.6",
          "protein_id": "ENSP00000401066.2",
          "transcript_support_level": 2,
          "aa_start": 595,
          "aa_end": null,
          "aa_length": 723,
          "cds_start": 1784,
          "cds_end": null,
          "cds_length": 2172,
          "cdna_start": 1832,
          "cdna_end": null,
          "cdna_length": 2460,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A6",
          "gene_hgnc_id": 14472,
          "hgvs_c": "c.1571T>C",
          "hgvs_p": "p.Met524Thr",
          "transcript": "NM_001281733.2",
          "protein_id": "NP_001268662.1",
          "transcript_support_level": null,
          "aa_start": 524,
          "aa_end": null,
          "aa_length": 651,
          "cds_start": 1571,
          "cds_end": null,
          "cds_length": 1956,
          "cdna_start": 1639,
          "cdna_end": null,
          "cdna_length": 2287,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A6",
          "gene_hgnc_id": 14472,
          "hgvs_c": "c.1571T>C",
          "hgvs_p": "p.Met524Thr",
          "transcript": "ENST00000337000.12",
          "protein_id": "ENSP00000337648.8",
          "transcript_support_level": 2,
          "aa_start": 524,
          "aa_end": null,
          "aa_length": 651,
          "cds_start": 1571,
          "cds_end": null,
          "cds_length": 1956,
          "cdna_start": 1671,
          "cdna_end": null,
          "cdna_length": 2317,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A6",
          "gene_hgnc_id": 14472,
          "hgvs_c": "n.407T>C",
          "hgvs_p": null,
          "transcript": "ENST00000462009.6",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 825,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A6",
          "gene_hgnc_id": 14472,
          "hgvs_c": "n.546T>C",
          "hgvs_p": null,
          "transcript": "ENST00000466257.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 706,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A6",
          "gene_hgnc_id": 14472,
          "hgvs_c": "n.4143T>C",
          "hgvs_p": null,
          "transcript": "ENST00000489483.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5682,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": 16,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A6",
          "gene_hgnc_id": 14472,
          "hgvs_c": "c.1819+468T>C",
          "hgvs_p": null,
          "transcript": "NM_134426.3",
          "protein_id": "NP_602298.2",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 740,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2223,
          "cdna_start": null,
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          "cdna_length": 2554,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000300190",
          "gene_hgnc_id": null,
          "hgvs_c": "n.259-517A>G",
          "hgvs_p": null,
          "transcript": "ENST00000769883.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 775,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A6",
          "gene_hgnc_id": 14472,
          "hgvs_c": "n.*222T>C",
          "hgvs_p": null,
          "transcript": "ENST00000469693.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 574,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "SLC26A6",
      "gene_hgnc_id": 14472,
      "dbsnp": "rs753810423",
      "frequency_reference_population": 0.00001135866,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 18,
      "gnomad_exomes_af": 0.00000767873,
      "gnomad_genomes_af": 0.0000460024,
      "gnomad_exomes_ac": 11,
      "gnomad_genomes_ac": 7,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.0823589563369751,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "dbscSNV1_RF",
      "revel_score": 0.221,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0835,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.33,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.203,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.04,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": 0.00000730739014186626,
      "dbscsnv_ada_prediction": "Benign",
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000395550.7",
          "gene_symbol": "SLC26A6",
          "hgnc_id": 14472,
          "effects": [
            "missense_variant",
            "splice_region_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1892T>C",
          "hgvs_p": "p.Met631Thr"
        },
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000769883.1",
          "gene_symbol": "ENSG00000300190",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.259-517A>G",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}