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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-49001557-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=49001557&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 49001557,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_177938.2",
      "consequences": [
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HTM",
          "gene_hgnc_id": 28858,
          "hgvs_c": "c.556A>G",
          "hgvs_p": "p.Met186Val",
          "transcript": "NM_177939.3",
          "protein_id": "NP_808808.1",
          "transcript_support_level": null,
          "aa_start": 186,
          "aa_end": null,
          "aa_length": 502,
          "cds_start": 556,
          "cds_end": null,
          "cds_length": 1509,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000383729.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_177939.3"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HTM",
          "gene_hgnc_id": 28858,
          "hgvs_c": "c.556A>G",
          "hgvs_p": "p.Met186Val",
          "transcript": "ENST00000383729.9",
          "protein_id": "ENSP00000373235.4",
          "transcript_support_level": 1,
          "aa_start": 186,
          "aa_end": null,
          "aa_length": 502,
          "cds_start": 556,
          "cds_end": null,
          "cds_length": 1509,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_177939.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000383729.9"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HTM",
          "gene_hgnc_id": 28858,
          "hgvs_c": "c.556A>G",
          "hgvs_p": "p.Met186Val",
          "transcript": "ENST00000343546.8",
          "protein_id": "ENSP00000341422.4",
          "transcript_support_level": 1,
          "aa_start": 186,
          "aa_end": null,
          "aa_length": 563,
          "cds_start": 556,
          "cds_end": null,
          "cds_length": 1692,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000343546.8"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HTM",
          "gene_hgnc_id": 28858,
          "hgvs_c": "n.485A>G",
          "hgvs_p": null,
          "transcript": "ENST00000485210.1",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000485210.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HTM",
          "gene_hgnc_id": 28858,
          "hgvs_c": "c.556A>G",
          "hgvs_p": "p.Met186Val",
          "transcript": "NM_177938.2",
          "protein_id": "NP_808807.2",
          "transcript_support_level": null,
          "aa_start": 186,
          "aa_end": null,
          "aa_length": 563,
          "cds_start": 556,
          "cds_end": null,
          "cds_length": 1692,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_177938.2"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HTM",
          "gene_hgnc_id": 28858,
          "hgvs_c": "c.679A>G",
          "hgvs_p": "p.Met227Val",
          "transcript": "ENST00000867728.1",
          "protein_id": "ENSP00000537787.1",
          "transcript_support_level": null,
          "aa_start": 227,
          "aa_end": null,
          "aa_length": 543,
          "cds_start": 679,
          "cds_end": null,
          "cds_length": 1632,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867728.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HTM",
          "gene_hgnc_id": 28858,
          "hgvs_c": "c.556A>G",
          "hgvs_p": "p.Met186Val",
          "transcript": "ENST00000867726.1",
          "protein_id": "ENSP00000537785.1",
          "transcript_support_level": null,
          "aa_start": 186,
          "aa_end": null,
          "aa_length": 531,
          "cds_start": 556,
          "cds_end": null,
          "cds_length": 1596,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867726.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HTM",
          "gene_hgnc_id": 28858,
          "hgvs_c": "c.631A>G",
          "hgvs_p": "p.Met211Val",
          "transcript": "ENST00000942776.1",
          "protein_id": "ENSP00000612835.1",
          "transcript_support_level": null,
          "aa_start": 211,
          "aa_end": null,
          "aa_length": 527,
          "cds_start": 631,
          "cds_end": null,
          "cds_length": 1584,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942776.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HTM",
          "gene_hgnc_id": 28858,
          "hgvs_c": "c.556A>G",
          "hgvs_p": "p.Met186Val",
          "transcript": "ENST00000867727.1",
          "protein_id": "ENSP00000537786.1",
          "transcript_support_level": null,
          "aa_start": 186,
          "aa_end": null,
          "aa_length": 516,
          "cds_start": 556,
          "cds_end": null,
          "cds_length": 1551,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867727.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HTM",
          "gene_hgnc_id": 28858,
          "hgvs_c": "c.556A>G",
          "hgvs_p": "p.Met186Val",
          "transcript": "ENST00000918330.1",
          "protein_id": "ENSP00000588389.1",
          "transcript_support_level": null,
          "aa_start": 186,
          "aa_end": null,
          "aa_length": 513,
          "cds_start": 556,
          "cds_end": null,
          "cds_length": 1542,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000918330.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HTM",
          "gene_hgnc_id": 28858,
          "hgvs_c": "c.556A>G",
          "hgvs_p": "p.Met186Val",
          "transcript": "ENST00000942775.1",
          "protein_id": "ENSP00000612834.1",
          "transcript_support_level": null,
          "aa_start": 186,
          "aa_end": null,
          "aa_length": 501,
          "cds_start": 556,
          "cds_end": null,
          "cds_length": 1506,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942775.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HTM",
          "gene_hgnc_id": 28858,
          "hgvs_c": "c.556A>G",
          "hgvs_p": "p.Met186Val",
          "transcript": "ENST00000942778.1",
          "protein_id": "ENSP00000612837.1",
          "transcript_support_level": null,
          "aa_start": 186,
          "aa_end": null,
          "aa_length": 498,
          "cds_start": 556,
          "cds_end": null,
          "cds_length": 1497,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942778.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HTM",
          "gene_hgnc_id": 28858,
          "hgvs_c": "c.556A>G",
          "hgvs_p": "p.Met186Val",
          "transcript": "ENST00000867725.1",
          "protein_id": "ENSP00000537784.1",
          "transcript_support_level": null,
          "aa_start": 186,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 556,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867725.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HTM",
          "gene_hgnc_id": 28858,
          "hgvs_c": "c.556A>G",
          "hgvs_p": "p.Met186Val",
          "transcript": "ENST00000942774.1",
          "protein_id": "ENSP00000612833.1",
          "transcript_support_level": null,
          "aa_start": 186,
          "aa_end": null,
          "aa_length": 466,
          "cds_start": 556,
          "cds_end": null,
          "cds_length": 1401,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942774.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HTM",
          "gene_hgnc_id": 28858,
          "hgvs_c": "c.556A>G",
          "hgvs_p": "p.Met186Val",
          "transcript": "ENST00000867729.1",
          "protein_id": "ENSP00000537788.1",
          "transcript_support_level": null,
          "aa_start": 186,
          "aa_end": null,
          "aa_length": 440,
          "cds_start": 556,
          "cds_end": null,
          "cds_length": 1323,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867729.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HTM",
          "gene_hgnc_id": 28858,
          "hgvs_c": "c.556A>G",
          "hgvs_p": "p.Met186Val",
          "transcript": "ENST00000867730.1",
          "protein_id": "ENSP00000537789.1",
          "transcript_support_level": null,
          "aa_start": 186,
          "aa_end": null,
          "aa_length": 404,
          "cds_start": 556,
          "cds_end": null,
          "cds_length": 1215,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867730.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HTM",
          "gene_hgnc_id": 28858,
          "hgvs_c": "c.556A>G",
          "hgvs_p": "p.Met186Val",
          "transcript": "ENST00000942777.1",
          "protein_id": "ENSP00000612836.1",
          "transcript_support_level": null,
          "aa_start": 186,
          "aa_end": null,
          "aa_length": 355,
          "cds_start": 556,
          "cds_end": null,
          "cds_length": 1068,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942777.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HTM",
          "gene_hgnc_id": 28858,
          "hgvs_c": "c.409A>G",
          "hgvs_p": "p.Met137Val",
          "transcript": "ENST00000444213.5",
          "protein_id": "ENSP00000398554.1",
          "transcript_support_level": 4,
          "aa_start": 137,
          "aa_end": null,
          "aa_length": 224,
          "cds_start": 409,
          "cds_end": null,
          "cds_length": 677,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000444213.5"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HTM",
          "gene_hgnc_id": 28858,
          "hgvs_c": "c.82A>G",
          "hgvs_p": "p.Met28Val",
          "transcript": "ENST00000491739.5",
          "protein_id": "ENSP00000476303.1",
          "transcript_support_level": 3,
          "aa_start": 28,
          "aa_end": null,
          "aa_length": 156,
          "cds_start": 82,
          "cds_end": null,
          "cds_length": 471,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000491739.5"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HTM",
          "gene_hgnc_id": 28858,
          "hgvs_c": "c.556A>G",
          "hgvs_p": "p.Met186Val",
          "transcript": "XM_047448367.1",
          "protein_id": "XP_047304323.1",
          "transcript_support_level": null,
          "aa_start": 186,
          "aa_end": null,
          "aa_length": 273,
          "cds_start": 556,
          "cds_end": null,
          "cds_length": 822,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047448367.1"
        },
        {
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        {
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      "gene_symbol": "P4HTM",
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      "dbsnp": "rs372281689",
      "frequency_reference_population": 0.000009914721,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 16,
      "gnomad_exomes_af": 0.00000957891,
      "gnomad_genomes_af": 0.0000131391,
      "gnomad_exomes_ac": 14,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.06206592917442322,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.112,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0646,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.46,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.563,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
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      "apogee2_score": null,
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      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_177938.2",
          "gene_symbol": "P4HTM",
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          "effects": [
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          "inheritance_mode": "AR",
          "hgvs_c": "c.556A>G",
          "hgvs_p": "p.Met186Val"
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      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}