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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-49098670-C-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=49098670&ref=C&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 49098670,
      "ref": "C",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_005051.3",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "QARS1",
          "gene_hgnc_id": 9751,
          "hgvs_c": "c.1886G>T",
          "hgvs_p": "p.Arg629Leu",
          "transcript": "NM_005051.3",
          "protein_id": "NP_005042.1",
          "transcript_support_level": null,
          "aa_start": 629,
          "aa_end": null,
          "aa_length": 775,
          "cds_start": 1886,
          "cds_end": null,
          "cds_length": 2328,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000306125.12",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_005051.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "QARS1",
          "gene_hgnc_id": 9751,
          "hgvs_c": "c.1886G>T",
          "hgvs_p": "p.Arg629Leu",
          "transcript": "ENST00000306125.12",
          "protein_id": "ENSP00000307567.6",
          "transcript_support_level": 1,
          "aa_start": 629,
          "aa_end": null,
          "aa_length": 775,
          "cds_start": 1886,
          "cds_end": null,
          "cds_length": 2328,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_005051.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000306125.12"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "QARS1",
          "gene_hgnc_id": 9751,
          "hgvs_c": "c.1451G>T",
          "hgvs_p": "p.Arg484Leu",
          "transcript": "ENST00000464962.6",
          "protein_id": "ENSP00000489011.1",
          "transcript_support_level": 1,
          "aa_start": 484,
          "aa_end": null,
          "aa_length": 630,
          "cds_start": 1451,
          "cds_end": null,
          "cds_length": 1893,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000464962.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "QARS1",
          "gene_hgnc_id": 9751,
          "hgvs_c": "c.2009G>T",
          "hgvs_p": "p.Arg670Leu",
          "transcript": "ENST00000965966.1",
          "protein_id": "ENSP00000636025.1",
          "transcript_support_level": null,
          "aa_start": 670,
          "aa_end": null,
          "aa_length": 816,
          "cds_start": 2009,
          "cds_end": null,
          "cds_length": 2451,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965966.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "QARS1",
          "gene_hgnc_id": 9751,
          "hgvs_c": "c.1886G>T",
          "hgvs_p": "p.Arg629Leu",
          "transcript": "ENST00000965963.1",
          "protein_id": "ENSP00000636022.1",
          "transcript_support_level": null,
          "aa_start": 629,
          "aa_end": null,
          "aa_length": 785,
          "cds_start": 1886,
          "cds_end": null,
          "cds_length": 2358,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965963.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "QARS1",
          "gene_hgnc_id": 9751,
          "hgvs_c": "c.1883G>T",
          "hgvs_p": "p.Arg628Leu",
          "transcript": "ENST00000870701.1",
          "protein_id": "ENSP00000540760.1",
          "transcript_support_level": null,
          "aa_start": 628,
          "aa_end": null,
          "aa_length": 774,
          "cds_start": 1883,
          "cds_end": null,
          "cds_length": 2325,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000870701.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "QARS1",
          "gene_hgnc_id": 9751,
          "hgvs_c": "c.1883G>T",
          "hgvs_p": "p.Arg628Leu",
          "transcript": "ENST00000965961.1",
          "protein_id": "ENSP00000636020.1",
          "transcript_support_level": null,
          "aa_start": 628,
          "aa_end": null,
          "aa_length": 774,
          "cds_start": 1883,
          "cds_end": null,
          "cds_length": 2325,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965961.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "QARS1",
          "gene_hgnc_id": 9751,
          "hgvs_c": "c.1853G>T",
          "hgvs_p": "p.Arg618Leu",
          "transcript": "NM_001272073.2",
          "protein_id": "NP_001259002.1",
          "transcript_support_level": null,
          "aa_start": 618,
          "aa_end": null,
          "aa_length": 764,
          "cds_start": 1853,
          "cds_end": null,
          "cds_length": 2295,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001272073.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "QARS1",
          "gene_hgnc_id": 9751,
          "hgvs_c": "c.1853G>T",
          "hgvs_p": "p.Arg618Leu",
          "transcript": "ENST00000414533.5",
          "protein_id": "ENSP00000390015.1",
          "transcript_support_level": 2,
          "aa_start": 618,
          "aa_end": null,
          "aa_length": 764,
          "cds_start": 1853,
          "cds_end": null,
          "cds_length": 2295,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000414533.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "QARS1",
          "gene_hgnc_id": 9751,
          "hgvs_c": "c.1835G>T",
          "hgvs_p": "p.Arg612Leu",
          "transcript": "ENST00000965960.1",
          "protein_id": "ENSP00000636019.1",
          "transcript_support_level": null,
          "aa_start": 612,
          "aa_end": null,
          "aa_length": 758,
          "cds_start": 1835,
          "cds_end": null,
          "cds_length": 2277,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965960.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "QARS1",
          "gene_hgnc_id": 9751,
          "hgvs_c": "c.1832G>T",
          "hgvs_p": "p.Arg611Leu",
          "transcript": "ENST00000965967.1",
          "protein_id": "ENSP00000636026.1",
          "transcript_support_level": null,
          "aa_start": 611,
          "aa_end": null,
          "aa_length": 757,
          "cds_start": 1832,
          "cds_end": null,
          "cds_length": 2274,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965967.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "QARS1",
          "gene_hgnc_id": 9751,
          "hgvs_c": "c.1814G>T",
          "hgvs_p": "p.Arg605Leu",
          "transcript": "ENST00000965965.1",
          "protein_id": "ENSP00000636024.1",
          "transcript_support_level": null,
          "aa_start": 605,
          "aa_end": null,
          "aa_length": 751,
          "cds_start": 1814,
          "cds_end": null,
          "cds_length": 2256,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965965.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "QARS1",
          "gene_hgnc_id": 9751,
          "hgvs_c": "c.1760G>T",
          "hgvs_p": "p.Arg587Leu",
          "transcript": "ENST00000965968.1",
          "protein_id": "ENSP00000636027.1",
          "transcript_support_level": null,
          "aa_start": 587,
          "aa_end": null,
          "aa_length": 733,
          "cds_start": 1760,
          "cds_end": null,
          "cds_length": 2202,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965968.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "QARS1",
          "gene_hgnc_id": 9751,
          "hgvs_c": "c.1700G>T",
          "hgvs_p": "p.Arg567Leu",
          "transcript": "ENST00000965962.1",
          "protein_id": "ENSP00000636021.1",
          "transcript_support_level": null,
          "aa_start": 567,
          "aa_end": null,
          "aa_length": 713,
          "cds_start": 1700,
          "cds_end": null,
          "cds_length": 2142,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965962.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "QARS1",
          "gene_hgnc_id": 9751,
          "hgvs_c": "c.1886G>T",
          "hgvs_p": "p.Arg629Leu",
          "transcript": "ENST00000913518.1",
          "protein_id": "ENSP00000583577.1",
          "transcript_support_level": null,
          "aa_start": 629,
          "aa_end": null,
          "aa_length": 704,
          "cds_start": 1886,
          "cds_end": null,
          "cds_length": 2115,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000913518.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "QARS1",
          "gene_hgnc_id": 9751,
          "hgvs_c": "c.446G>T",
          "hgvs_p": "p.Arg149Leu",
          "transcript": "ENST00000453392.5",
          "protein_id": "ENSP00000396326.1",
          "transcript_support_level": 5,
          "aa_start": 149,
          "aa_end": null,
          "aa_length": 253,
          "cds_start": 446,
          "cds_end": null,
          "cds_length": 762,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000453392.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "QARS1",
          "gene_hgnc_id": 9751,
          "hgvs_c": "c.128G>T",
          "hgvs_p": "p.Arg43Leu",
          "transcript": "ENST00000466179.2",
          "protein_id": "ENSP00000489462.1",
          "transcript_support_level": 3,
          "aa_start": 43,
          "aa_end": null,
          "aa_length": 91,
          "cds_start": 128,
          "cds_end": null,
          "cds_length": 276,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000466179.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "QARS1",
          "gene_hgnc_id": 9751,
          "hgvs_c": "c.1886G>T",
          "hgvs_p": "p.Arg629Leu",
          "transcript": "XM_017006965.3",
          "protein_id": "XP_016862454.2",
          "transcript_support_level": null,
          "aa_start": 629,
          "aa_end": null,
          "aa_length": 733,
          "cds_start": 1886,
          "cds_end": null,
          "cds_length": 2202,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017006965.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": 10,
          "intron_rank_end": null,
          "gene_symbol": "QARS1",
          "gene_hgnc_id": 9751,
          "hgvs_c": "c.877-2591G>T",
          "hgvs_p": null,
          "transcript": "ENST00000965964.1",
          "protein_id": "ENSP00000636023.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 308,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 927,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965964.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "QARS1",
          "gene_hgnc_id": 9751,
          "hgvs_c": "n.*1534G>T",
          "hgvs_p": null,
          "transcript": "ENST00000430182.5",
          "protein_id": "ENSP00000389823.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
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      "frequency_reference_population": 6.8866257e-7,
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      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.88663e-7,
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      "gnomad_exomes_homalt": 0,
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      "computational_score_selected": 0.9447182416915894,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "revel_prediction": "Uncertain_significance",
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      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.2,
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      "phylop100way_score": 7.413,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
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      "acmg_score": 6,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM2,PP3_Strong",
      "acmg_by_gene": [
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          "verdict": "Likely_pathogenic",
          "transcript": "NM_005051.3",
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          "hgvs_p": "p.Arg629Leu"
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      ],
      "clinvar_disease": "Diffuse cerebral and cerebellar atrophy - intractable seizures - progressive microcephaly syndrome",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "Diffuse cerebral and cerebellar atrophy - intractable seizures - progressive microcephaly syndrome",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}