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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-49280761-G-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=49280761&ref=G&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 49280761,
      "ref": "G",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_003363.4",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP4",
          "gene_hgnc_id": 12627,
          "hgvs_c": "c.2627C>A",
          "hgvs_p": "p.Ala876Asp",
          "transcript": "NM_003363.4",
          "protein_id": "NP_003354.2",
          "transcript_support_level": null,
          "aa_start": 876,
          "aa_end": null,
          "aa_length": 963,
          "cds_start": 2627,
          "cds_end": null,
          "cds_length": 2892,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000265560.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_003363.4"
        },
        {
          "aa_ref": "A",
          "aa_alt": "D",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP4",
          "gene_hgnc_id": 12627,
          "hgvs_c": "c.2627C>A",
          "hgvs_p": "p.Ala876Asp",
          "transcript": "ENST00000265560.9",
          "protein_id": "ENSP00000265560.4",
          "transcript_support_level": 1,
          "aa_start": 876,
          "aa_end": null,
          "aa_length": 963,
          "cds_start": 2627,
          "cds_end": null,
          "cds_length": 2892,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_003363.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000265560.9"
        },
        {
          "aa_ref": "A",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP4",
          "gene_hgnc_id": 12627,
          "hgvs_c": "c.2486C>A",
          "hgvs_p": "p.Ala829Asp",
          "transcript": "ENST00000351842.8",
          "protein_id": "ENSP00000341028.4",
          "transcript_support_level": 1,
          "aa_start": 829,
          "aa_end": null,
          "aa_length": 916,
          "cds_start": 2486,
          "cds_end": null,
          "cds_length": 2751,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000351842.8"
        },
        {
          "aa_ref": "A",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP4",
          "gene_hgnc_id": 12627,
          "hgvs_c": "c.2780C>A",
          "hgvs_p": "p.Ala927Asp",
          "transcript": "ENST00000911610.1",
          "protein_id": "ENSP00000581669.1",
          "transcript_support_level": null,
          "aa_start": 927,
          "aa_end": null,
          "aa_length": 1014,
          "cds_start": 2780,
          "cds_end": null,
          "cds_length": 3045,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000911610.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP4",
          "gene_hgnc_id": 12627,
          "hgvs_c": "c.2756C>A",
          "hgvs_p": "p.Ala919Asp",
          "transcript": "ENST00000965332.1",
          "protein_id": "ENSP00000635391.1",
          "transcript_support_level": null,
          "aa_start": 919,
          "aa_end": null,
          "aa_length": 1006,
          "cds_start": 2756,
          "cds_end": null,
          "cds_length": 3021,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965332.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP4",
          "gene_hgnc_id": 12627,
          "hgvs_c": "c.2738C>A",
          "hgvs_p": "p.Ala913Asp",
          "transcript": "ENST00000965340.1",
          "protein_id": "ENSP00000635399.1",
          "transcript_support_level": null,
          "aa_start": 913,
          "aa_end": null,
          "aa_length": 1000,
          "cds_start": 2738,
          "cds_end": null,
          "cds_length": 3003,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965340.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP4",
          "gene_hgnc_id": 12627,
          "hgvs_c": "c.2705C>A",
          "hgvs_p": "p.Ala902Asp",
          "transcript": "ENST00000877491.1",
          "protein_id": "ENSP00000547550.1",
          "transcript_support_level": null,
          "aa_start": 902,
          "aa_end": null,
          "aa_length": 989,
          "cds_start": 2705,
          "cds_end": null,
          "cds_length": 2970,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000877491.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP4",
          "gene_hgnc_id": 12627,
          "hgvs_c": "c.2648C>A",
          "hgvs_p": "p.Ala883Asp",
          "transcript": "ENST00000965341.1",
          "protein_id": "ENSP00000635400.1",
          "transcript_support_level": null,
          "aa_start": 883,
          "aa_end": null,
          "aa_length": 970,
          "cds_start": 2648,
          "cds_end": null,
          "cds_length": 2913,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965341.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP4",
          "gene_hgnc_id": 12627,
          "hgvs_c": "c.2639C>A",
          "hgvs_p": "p.Ala880Asp",
          "transcript": "ENST00000911611.1",
          "protein_id": "ENSP00000581670.1",
          "transcript_support_level": null,
          "aa_start": 880,
          "aa_end": null,
          "aa_length": 967,
          "cds_start": 2639,
          "cds_end": null,
          "cds_length": 2904,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000911611.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP4",
          "gene_hgnc_id": 12627,
          "hgvs_c": "c.2624C>A",
          "hgvs_p": "p.Ala875Asp",
          "transcript": "ENST00000911612.1",
          "protein_id": "ENSP00000581671.1",
          "transcript_support_level": null,
          "aa_start": 875,
          "aa_end": null,
          "aa_length": 962,
          "cds_start": 2624,
          "cds_end": null,
          "cds_length": 2889,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000911612.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP4",
          "gene_hgnc_id": 12627,
          "hgvs_c": "c.2624C>A",
          "hgvs_p": "p.Ala875Asp",
          "transcript": "ENST00000965324.1",
          "protein_id": "ENSP00000635383.1",
          "transcript_support_level": null,
          "aa_start": 875,
          "aa_end": null,
          "aa_length": 962,
          "cds_start": 2624,
          "cds_end": null,
          "cds_length": 2889,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965324.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP4",
          "gene_hgnc_id": 12627,
          "hgvs_c": "c.2621C>A",
          "hgvs_p": "p.Ala874Asp",
          "transcript": "ENST00000877475.1",
          "protein_id": "ENSP00000547534.1",
          "transcript_support_level": null,
          "aa_start": 874,
          "aa_end": null,
          "aa_length": 961,
          "cds_start": 2621,
          "cds_end": null,
          "cds_length": 2886,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000877475.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP4",
          "gene_hgnc_id": 12627,
          "hgvs_c": "c.2594C>A",
          "hgvs_p": "p.Ala865Asp",
          "transcript": "ENST00000877488.1",
          "protein_id": "ENSP00000547547.1",
          "transcript_support_level": null,
          "aa_start": 865,
          "aa_end": null,
          "aa_length": 952,
          "cds_start": 2594,
          "cds_end": null,
          "cds_length": 2859,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000877488.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP4",
          "gene_hgnc_id": 12627,
          "hgvs_c": "c.2585C>A",
          "hgvs_p": "p.Ala862Asp",
          "transcript": "ENST00000965327.1",
          "protein_id": "ENSP00000635386.1",
          "transcript_support_level": null,
          "aa_start": 862,
          "aa_end": null,
          "aa_length": 949,
          "cds_start": 2585,
          "cds_end": null,
          "cds_length": 2850,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965327.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP4",
          "gene_hgnc_id": 12627,
          "hgvs_c": "c.2573C>A",
          "hgvs_p": "p.Ala858Asp",
          "transcript": "ENST00000877477.1",
          "protein_id": "ENSP00000547536.1",
          "transcript_support_level": null,
          "aa_start": 858,
          "aa_end": null,
          "aa_length": 945,
          "cds_start": 2573,
          "cds_end": null,
          "cds_length": 2838,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000877477.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP4",
          "gene_hgnc_id": 12627,
          "hgvs_c": "c.2558C>A",
          "hgvs_p": "p.Ala853Asp",
          "transcript": "ENST00000965330.1",
          "protein_id": "ENSP00000635389.1",
          "transcript_support_level": null,
          "aa_start": 853,
          "aa_end": null,
          "aa_length": 940,
          "cds_start": 2558,
          "cds_end": null,
          "cds_length": 2823,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965330.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP4",
          "gene_hgnc_id": 12627,
          "hgvs_c": "c.2513C>A",
          "hgvs_p": "p.Ala838Asp",
          "transcript": "ENST00000877480.1",
          "protein_id": "ENSP00000547539.1",
          "transcript_support_level": null,
          "aa_start": 838,
          "aa_end": null,
          "aa_length": 925,
          "cds_start": 2513,
          "cds_end": null,
          "cds_length": 2778,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000877480.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP4",
          "gene_hgnc_id": 12627,
          "hgvs_c": "c.2486C>A",
          "hgvs_p": "p.Ala829Asp",
          "transcript": "NM_199443.3",
          "protein_id": "NP_955475.1",
          "transcript_support_level": null,
          "aa_start": 829,
          "aa_end": null,
          "aa_length": 916,
          "cds_start": 2486,
          "cds_end": null,
          "cds_length": 2751,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_199443.3"
        },
        {
          "aa_ref": "A",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP4",
          "gene_hgnc_id": 12627,
          "hgvs_c": "c.2483C>A",
          "hgvs_p": "p.Ala828Asp",
          "transcript": "ENST00000877484.1",
          "protein_id": "ENSP00000547543.1",
          "transcript_support_level": null,
          "aa_start": 828,
          "aa_end": null,
          "aa_length": 915,
          "cds_start": 2483,
          "cds_end": null,
          "cds_length": 2748,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000877484.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP4",
          "gene_hgnc_id": 12627,
          "hgvs_c": "c.2480C>A",
          "hgvs_p": "p.Ala827Asp",
          "transcript": "ENST00000877485.1",
          "protein_id": "ENSP00000547544.1",
          "transcript_support_level": null,
          "aa_start": 827,
          "aa_end": null,
          "aa_length": 914,
          "cds_start": 2480,
          "cds_end": null,
          "cds_length": 2745,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
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        {
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      ],
      "gene_symbol": "USP4",
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      "dbsnp": "rs775772757",
      "frequency_reference_population": 0.000005576678,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 9,
      "gnomad_exomes_af": 0.00000273645,
      "gnomad_genomes_af": 0.0000328697,
      "gnomad_exomes_ac": 4,
      "gnomad_genomes_ac": 5,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.6298955082893372,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.194,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.9514,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.3,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 4.144,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_003363.4",
          "gene_symbol": "USP4",
          "hgnc_id": 12627,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2627C>A",
          "hgvs_p": "p.Ala876Asp"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}