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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-50274469-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=50274469&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "message": null,
  "variants": [
    {
      "acmg_by_gene": [
        {
          "benign_score": 13,
          "criteria": [
            "BP4_Strong",
            "BP6",
            "BA1"
          ],
          "effects": [
            "missense_variant"
          ],
          "gene_symbol": "SEMA3B",
          "hgnc_id": 10724,
          "hgvs_c": "c.1259C>T",
          "hgvs_p": "p.Thr420Ile",
          "inheritance_mode": "",
          "pathogenic_score": 0,
          "score": -13,
          "transcript": "NM_001290061.1",
          "verdict": "Benign"
        },
        {
          "benign_score": 13,
          "criteria": [
            "BP4_Strong",
            "BP6",
            "BA1"
          ],
          "effects": [
            "intron_variant"
          ],
          "gene_symbol": "SEMA3B-AS1",
          "hgnc_id": 49096,
          "hgvs_c": "n.80-1256G>A",
          "hgvs_p": null,
          "inheritance_mode": "",
          "pathogenic_score": 0,
          "score": -13,
          "transcript": "ENST00000736630.1",
          "verdict": "Benign"
        }
      ],
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6,BA1",
      "acmg_score": -13,
      "allele_count_reference_population": 63385,
      "alphamissense_prediction": null,
      "alphamissense_score": 0.0491,
      "alt": "T",
      "apogee2_prediction": null,
      "apogee2_score": null,
      "bayesdelnoaf_prediction": "Pathogenic",
      "bayesdelnoaf_score": 0.44,
      "chr": "3",
      "clinvar_classification": "Benign",
      "clinvar_disease": "SEMA3B-related disorder",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "computational_prediction_selected": "Benign",
      "computational_score_selected": 0.000012084378795407247,
      "computational_source_selected": "MetaRNN",
      "consequences": [
        {
          "aa_alt": "I",
          "aa_end": null,
          "aa_length": 749,
          "aa_ref": "T",
          "aa_start": 415,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3184,
          "cdna_start": 1338,
          "cds_end": null,
          "cds_length": 2250,
          "cds_start": 1244,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 17,
          "exon_rank": 11,
          "exon_rank_end": null,
          "feature": "NM_001290060.2",
          "gene_hgnc_id": 10724,
          "gene_symbol": "SEMA3B",
          "hgvs_c": "c.1244C>T",
          "hgvs_p": "p.Thr415Ile",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "ENST00000616701.5",
          "protein_coding": true,
          "protein_id": "NP_001276989.1",
          "strand": true,
          "transcript": "NM_001290060.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": "I",
          "aa_end": null,
          "aa_length": 749,
          "aa_ref": "T",
          "aa_start": 415,
          "biotype": "protein_coding",
          "canonical": true,
          "cdna_end": null,
          "cdna_length": 3184,
          "cdna_start": 1338,
          "cds_end": null,
          "cds_length": 2250,
          "cds_start": 1244,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 17,
          "exon_rank": 11,
          "exon_rank_end": null,
          "feature": "ENST00000616701.5",
          "gene_hgnc_id": 10724,
          "gene_symbol": "SEMA3B",
          "hgvs_c": "c.1244C>T",
          "hgvs_p": "p.Thr415Ile",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "NM_001290060.2",
          "protein_coding": true,
          "protein_id": "ENSP00000484146.1",
          "strand": true,
          "transcript": "ENST00000616701.5",
          "transcript_support_level": 1
        },
        {
          "aa_alt": "I",
          "aa_end": null,
          "aa_length": 754,
          "aa_ref": "T",
          "aa_start": 420,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2292,
          "cdna_start": 1286,
          "cds_end": null,
          "cds_length": 2265,
          "cds_start": 1259,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 17,
          "exon_rank": 11,
          "exon_rank_end": null,
          "feature": "ENST00000611067.4",
          "gene_hgnc_id": 10724,
          "gene_symbol": "SEMA3B",
          "hgvs_c": "c.1259C>T",
          "hgvs_p": "p.Thr420Ile",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000480680.1",
          "strand": true,
          "transcript": "ENST00000611067.4",
          "transcript_support_level": 1
        },
        {
          "aa_alt": "I",
          "aa_end": null,
          "aa_length": 748,
          "aa_ref": "T",
          "aa_start": 414,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2755,
          "cdna_start": 1313,
          "cds_end": null,
          "cds_length": 2247,
          "cds_start": 1241,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 17,
          "exon_rank": 11,
          "exon_rank_end": null,
          "feature": "ENST00000433753.4",
          "gene_hgnc_id": 10724,
          "gene_symbol": "SEMA3B",
          "hgvs_c": "c.1241C>T",
          "hgvs_p": "p.Thr414Ile",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000485281.1",
          "strand": true,
          "transcript": "ENST00000433753.4",
          "transcript_support_level": 1
        },
        {
          "aa_alt": "I",
          "aa_end": null,
          "aa_length": 406,
          "aa_ref": "T",
          "aa_start": 72,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2221,
          "cdna_start": 756,
          "cds_end": null,
          "cds_length": 1221,
          "cds_start": 215,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 11,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "ENST00000456560.6",
          "gene_hgnc_id": 10724,
          "gene_symbol": "SEMA3B",
          "hgvs_c": "c.215C>T",
          "hgvs_p": "p.Thr72Ile",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000485646.1",
          "strand": true,
          "transcript": "ENST00000456560.6",
          "transcript_support_level": 1
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "retained_intron",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2710,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_count": 14,
          "exon_rank": 8,
          "exon_rank_end": null,
          "feature": "ENST00000441915.5",
          "gene_hgnc_id": 10724,
          "gene_symbol": "SEMA3B",
          "hgvs_c": "n.1240C>T",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": true,
          "transcript": "ENST00000441915.5",
          "transcript_support_level": 1
        },
        {
          "aa_alt": "I",
          "aa_end": null,
          "aa_length": 756,
          "aa_ref": "T",
          "aa_start": 415,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2824,
          "cdna_start": 1338,
          "cds_end": null,
          "cds_length": 2271,
          "cds_start": 1244,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 17,
          "exon_rank": 11,
          "exon_rank_end": null,
          "feature": "ENST00000942600.1",
          "gene_hgnc_id": 10724,
          "gene_symbol": "SEMA3B",
          "hgvs_c": "c.1244C>T",
          "hgvs_p": "p.Thr415Ile",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000612659.1",
          "strand": true,
          "transcript": "ENST00000942600.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "I",
          "aa_end": null,
          "aa_length": 755,
          "aa_ref": "T",
          "aa_start": 414,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3023,
          "cdna_start": 1532,
          "cds_end": null,
          "cds_length": 2268,
          "cds_start": 1241,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 19,
          "exon_rank": 13,
          "exon_rank_end": null,
          "feature": "ENST00000942595.1",
          "gene_hgnc_id": 10724,
          "gene_symbol": "SEMA3B",
          "hgvs_c": "c.1241C>T",
          "hgvs_p": "p.Thr414Ile",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000612654.1",
          "strand": true,
          "transcript": "ENST00000942595.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "I",
          "aa_end": null,
          "aa_length": 754,
          "aa_ref": "T",
          "aa_start": 420,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2810,
          "cdna_start": 1339,
          "cds_end": null,
          "cds_length": 2265,
          "cds_start": 1259,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 17,
          "exon_rank": 11,
          "exon_rank_end": null,
          "feature": "NM_001290061.1",
          "gene_hgnc_id": 10724,
          "gene_symbol": "SEMA3B",
          "hgvs_c": "c.1259C>T",
          "hgvs_p": "p.Thr420Ile",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "NP_001276990.1",
          "strand": true,
          "transcript": "NM_001290061.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "I",
          "aa_end": null,
          "aa_length": 749,
          "aa_ref": "T",
          "aa_start": 415,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3408,
          "cdna_start": 1943,
          "cds_end": null,
          "cds_length": 2250,
          "cds_start": 1244,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 20,
          "exon_rank": 14,
          "exon_rank_end": null,
          "feature": "NM_001435956.1",
          "gene_hgnc_id": 10724,
          "gene_symbol": "SEMA3B",
          "hgvs_c": "c.1244C>T",
          "hgvs_p": "p.Thr415Ile",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "NP_001422885.1",
          "strand": true,
          "transcript": "NM_001435956.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "I",
          "aa_end": null,
          "aa_length": 749,
          "aa_ref": "T",
          "aa_start": 415,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3288,
          "cdna_start": 1442,
          "cds_end": null,
          "cds_length": 2250,
          "cds_start": 1244,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 18,
          "exon_rank": 12,
          "exon_rank_end": null,
          "feature": "NM_004636.4",
          "gene_hgnc_id": 10724,
          "gene_symbol": "SEMA3B",
          "hgvs_c": "c.1244C>T",
          "hgvs_p": "p.Thr415Ile",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "NP_004627.1",
          "strand": true,
          "transcript": "NM_004636.4",
          "transcript_support_level": null
        },
        {
          "aa_alt": "I",
          "aa_end": null,
          "aa_length": 749,
          "aa_ref": "T",
          "aa_start": 415,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2925,
          "cdna_start": 1486,
          "cds_end": null,
          "cds_length": 2250,
          "cds_start": 1244,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 18,
          "exon_rank": 12,
          "exon_rank_end": null,
          "feature": "ENST00000618865.4",
          "gene_hgnc_id": 10724,
          "gene_symbol": "SEMA3B",
          "hgvs_c": "c.1244C>T",
          "hgvs_p": "p.Thr415Ile",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000481957.1",
          "strand": true,
          "transcript": "ENST00000618865.4",
          "transcript_support_level": 5
        },
        {
          "aa_alt": "I",
          "aa_end": null,
          "aa_length": 749,
          "aa_ref": "T",
          "aa_start": 415,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3114,
          "cdna_start": 1646,
          "cds_end": null,
          "cds_length": 2250,
          "cds_start": 1244,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 19,
          "exon_rank": 13,
          "exon_rank_end": null,
          "feature": "ENST00000852511.1",
          "gene_hgnc_id": 10724,
          "gene_symbol": "SEMA3B",
          "hgvs_c": "c.1244C>T",
          "hgvs_p": "p.Thr415Ile",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000522570.1",
          "strand": true,
          "transcript": "ENST00000852511.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "I",
          "aa_end": null,
          "aa_length": 749,
          "aa_ref": "T",
          "aa_start": 415,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2916,
          "cdna_start": 1446,
          "cds_end": null,
          "cds_length": 2250,
          "cds_start": 1244,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 18,
          "exon_rank": 12,
          "exon_rank_end": null,
          "feature": "ENST00000852512.1",
          "gene_hgnc_id": 10724,
          "gene_symbol": "SEMA3B",
          "hgvs_c": "c.1244C>T",
          "hgvs_p": "p.Thr415Ile",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000522571.1",
          "strand": true,
          "transcript": "ENST00000852512.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "I",
          "aa_end": null,
          "aa_length": 749,
          "aa_ref": "T",
          "aa_start": 415,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3032,
          "cdna_start": 1562,
          "cds_end": null,
          "cds_length": 2250,
          "cds_start": 1244,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 18,
          "exon_rank": 12,
          "exon_rank_end": null,
          "feature": "ENST00000852513.1",
          "gene_hgnc_id": 10724,
          "gene_symbol": "SEMA3B",
          "hgvs_c": "c.1244C>T",
          "hgvs_p": "p.Thr415Ile",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000522572.1",
          "strand": true,
          "transcript": "ENST00000852513.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "I",
          "aa_end": null,
          "aa_length": 749,
          "aa_ref": "T",
          "aa_start": 415,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2820,
          "cdna_start": 1351,
          "cds_end": null,
          "cds_length": 2250,
          "cds_start": 1244,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 18,
          "exon_rank": 12,
          "exon_rank_end": null,
          "feature": "ENST00000852514.1",
          "gene_hgnc_id": 10724,
          "gene_symbol": "SEMA3B",
          "hgvs_c": "c.1244C>T",
          "hgvs_p": "p.Thr415Ile",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000522573.1",
          "strand": true,
          "transcript": "ENST00000852514.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "I",
          "aa_end": null,
          "aa_length": 749,
          "aa_ref": "T",
          "aa_start": 415,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2835,
          "cdna_start": 1370,
          "cds_end": null,
          "cds_length": 2250,
          "cds_start": 1244,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 18,
          "exon_rank": 12,
          "exon_rank_end": null,
          "feature": "ENST00000852515.1",
          "gene_hgnc_id": 10724,
          "gene_symbol": "SEMA3B",
          "hgvs_c": "c.1244C>T",
          "hgvs_p": "p.Thr415Ile",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000522574.1",
          "strand": true,
          "transcript": "ENST00000852515.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "I",
          "aa_end": null,
          "aa_length": 749,
          "aa_ref": "T",
          "aa_start": 415,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2849,
          "cdna_start": 1384,
          "cds_end": null,
          "cds_length": 2250,
          "cds_start": 1244,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 18,
          "exon_rank": 12,
          "exon_rank_end": null,
          "feature": "ENST00000852516.1",
          "gene_hgnc_id": 10724,
          "gene_symbol": "SEMA3B",
          "hgvs_c": "c.1244C>T",
          "hgvs_p": "p.Thr415Ile",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000522575.1",
          "strand": true,
          "transcript": "ENST00000852516.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "I",
          "aa_end": null,
          "aa_length": 749,
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      "splice_source_selected": "max_spliceai",
      "spliceai_max_prediction": "Benign",
      "spliceai_max_score": 0.04,
      "transcript": "NM_001290061.1"
    }
  ]
}
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