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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-56729485-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=56729485&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 56729485,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001128615.2",
      "consequences": [
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF3",
          "gene_hgnc_id": 683,
          "hgvs_c": "c.1366G>A",
          "hgvs_p": "p.Gly456Arg",
          "transcript": "NM_019555.3",
          "protein_id": "NP_062455.1",
          "transcript_support_level": null,
          "aa_start": 456,
          "aa_end": null,
          "aa_length": 526,
          "cds_start": 1366,
          "cds_end": null,
          "cds_length": 1581,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000296315.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_019555.3"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF3",
          "gene_hgnc_id": 683,
          "hgvs_c": "c.1366G>A",
          "hgvs_p": "p.Gly456Arg",
          "transcript": "ENST00000296315.8",
          "protein_id": "ENSP00000296315.3",
          "transcript_support_level": 1,
          "aa_start": 456,
          "aa_end": null,
          "aa_length": 526,
          "cds_start": 1366,
          "cds_end": null,
          "cds_length": 1581,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_019555.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000296315.8"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF3",
          "gene_hgnc_id": 683,
          "hgvs_c": "c.1462G>A",
          "hgvs_p": "p.Gly488Arg",
          "transcript": "ENST00000338458.8",
          "protein_id": "ENSP00000341071.4",
          "transcript_support_level": 1,
          "aa_start": 488,
          "aa_end": null,
          "aa_length": 558,
          "cds_start": 1462,
          "cds_end": null,
          "cds_length": 1677,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000338458.8"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF3",
          "gene_hgnc_id": 683,
          "hgvs_c": "c.1384G>A",
          "hgvs_p": "p.Gly462Arg",
          "transcript": "ENST00000413728.6",
          "protein_id": "ENSP00000410922.2",
          "transcript_support_level": 1,
          "aa_start": 462,
          "aa_end": null,
          "aa_length": 532,
          "cds_start": 1384,
          "cds_end": null,
          "cds_length": 1599,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000413728.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF3",
          "gene_hgnc_id": 683,
          "hgvs_c": "n.*731G>A",
          "hgvs_p": null,
          "transcript": "ENST00000465659.5",
          "protein_id": "ENSP00000417810.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000465659.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF3",
          "gene_hgnc_id": 683,
          "hgvs_c": "n.*731G>A",
          "hgvs_p": null,
          "transcript": "ENST00000465659.5",
          "protein_id": "ENSP00000417810.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000465659.5"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF3",
          "gene_hgnc_id": 683,
          "hgvs_c": "c.1462G>A",
          "hgvs_p": "p.Gly488Arg",
          "transcript": "NM_001128615.2",
          "protein_id": "NP_001122087.1",
          "transcript_support_level": null,
          "aa_start": 488,
          "aa_end": null,
          "aa_length": 558,
          "cds_start": 1462,
          "cds_end": null,
          "cds_length": 1677,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001128615.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF3",
          "gene_hgnc_id": 683,
          "hgvs_c": "c.1462G>A",
          "hgvs_p": "p.Gly488Arg",
          "transcript": "NM_001377407.1",
          "protein_id": "NP_001364336.1",
          "transcript_support_level": null,
          "aa_start": 488,
          "aa_end": null,
          "aa_length": 558,
          "cds_start": 1462,
          "cds_end": null,
          "cds_length": 1677,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001377407.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF3",
          "gene_hgnc_id": 683,
          "hgvs_c": "c.1402G>A",
          "hgvs_p": "p.Gly468Arg",
          "transcript": "NM_001377408.1",
          "protein_id": "NP_001364337.1",
          "transcript_support_level": null,
          "aa_start": 468,
          "aa_end": null,
          "aa_length": 538,
          "cds_start": 1402,
          "cds_end": null,
          "cds_length": 1617,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001377408.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF3",
          "gene_hgnc_id": 683,
          "hgvs_c": "c.1402G>A",
          "hgvs_p": "p.Gly468Arg",
          "transcript": "NM_001377409.1",
          "protein_id": "NP_001364338.1",
          "transcript_support_level": null,
          "aa_start": 468,
          "aa_end": null,
          "aa_length": 538,
          "cds_start": 1402,
          "cds_end": null,
          "cds_length": 1617,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001377409.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF3",
          "gene_hgnc_id": 683,
          "hgvs_c": "c.1402G>A",
          "hgvs_p": "p.Gly468Arg",
          "transcript": "NM_001377410.1",
          "protein_id": "NP_001364339.1",
          "transcript_support_level": null,
          "aa_start": 468,
          "aa_end": null,
          "aa_length": 538,
          "cds_start": 1402,
          "cds_end": null,
          "cds_length": 1617,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001377410.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF3",
          "gene_hgnc_id": 683,
          "hgvs_c": "c.1399G>A",
          "hgvs_p": "p.Gly467Arg",
          "transcript": "ENST00000863032.1",
          "protein_id": "ENSP00000533091.1",
          "transcript_support_level": null,
          "aa_start": 467,
          "aa_end": null,
          "aa_length": 537,
          "cds_start": 1399,
          "cds_end": null,
          "cds_length": 1614,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000863032.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF3",
          "gene_hgnc_id": 683,
          "hgvs_c": "c.1384G>A",
          "hgvs_p": "p.Gly462Arg",
          "transcript": "NM_001128616.2",
          "protein_id": "NP_001122088.1",
          "transcript_support_level": null,
          "aa_start": 462,
          "aa_end": null,
          "aa_length": 532,
          "cds_start": 1384,
          "cds_end": null,
          "cds_length": 1599,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001128616.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF3",
          "gene_hgnc_id": 683,
          "hgvs_c": "c.1384G>A",
          "hgvs_p": "p.Gly462Arg",
          "transcript": "NM_001289698.2",
          "protein_id": "NP_001276627.1",
          "transcript_support_level": null,
          "aa_start": 462,
          "aa_end": null,
          "aa_length": 532,
          "cds_start": 1384,
          "cds_end": null,
          "cds_length": 1599,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001289698.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF3",
          "gene_hgnc_id": 683,
          "hgvs_c": "c.1384G>A",
          "hgvs_p": "p.Gly462Arg",
          "transcript": "ENST00000496106.5",
          "protein_id": "ENSP00000420420.1",
          "transcript_support_level": 2,
          "aa_start": 462,
          "aa_end": null,
          "aa_length": 532,
          "cds_start": 1384,
          "cds_end": null,
          "cds_length": 1599,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000496106.5"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF3",
          "gene_hgnc_id": 683,
          "hgvs_c": "c.1369G>A",
          "hgvs_p": "p.Gly457Arg",
          "transcript": "NM_001377411.1",
          "protein_id": "NP_001364340.1",
          "transcript_support_level": null,
          "aa_start": 457,
          "aa_end": null,
          "aa_length": 527,
          "cds_start": 1369,
          "cds_end": null,
          "cds_length": 1584,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001377411.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF3",
          "gene_hgnc_id": 683,
          "hgvs_c": "c.1369G>A",
          "hgvs_p": "p.Gly457Arg",
          "transcript": "NM_001377412.1",
          "protein_id": "NP_001364341.1",
          "transcript_support_level": null,
          "aa_start": 457,
          "aa_end": null,
          "aa_length": 527,
          "cds_start": 1369,
          "cds_end": null,
          "cds_length": 1584,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001377412.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF3",
          "gene_hgnc_id": 683,
          "hgvs_c": "c.1279G>A",
          "hgvs_p": "p.Gly427Arg",
          "transcript": "ENST00000497267.5",
          "protein_id": "ENSP00000418826.1",
          "transcript_support_level": 2,
          "aa_start": 427,
          "aa_end": null,
          "aa_length": 497,
          "cds_start": 1279,
          "cds_end": null,
          "cds_length": 1494,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000497267.5"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF3",
          "gene_hgnc_id": 683,
          "hgvs_c": "c.1258G>A",
          "hgvs_p": "p.Gly420Arg",
          "transcript": "ENST00000863034.1",
          "protein_id": "ENSP00000533093.1",
          "transcript_support_level": null,
          "aa_start": 420,
          "aa_end": null,
          "aa_length": 490,
          "cds_start": 1258,
          "cds_end": null,
          "cds_length": 1473,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000863034.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF3",
          "gene_hgnc_id": 683,
          "hgvs_c": "c.1192G>A",
          "hgvs_p": "p.Gly398Arg",
          "transcript": "ENST00000863033.1",
          "protein_id": "ENSP00000533092.1",
          "transcript_support_level": null,
          "aa_start": 398,
          "aa_end": null,
          "aa_length": 468,
          "cds_start": 1192,
          "cds_end": null,
          "cds_length": 1407,
          "cdna_start": null,
          "cdna_end": null,
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      ],
      "gene_symbol": "ARHGEF3",
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      "dbsnp": "rs139172951",
      "frequency_reference_population": 0.00002416345,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 39,
      "gnomad_exomes_af": 0.0000129969,
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      "gnomad_exomes_ac": 19,
      "gnomad_genomes_ac": 20,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.10594731569290161,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.18,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1017,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.06,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 2.338,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
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      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -6,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate,BS2",
      "acmg_by_gene": [
        {
          "score": -6,
          "benign_score": 6,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001128615.2",
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          "effects": [
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          "hgvs_p": "p.Gly488Arg"
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}