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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-69959325-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=69959325&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 69959325,
      "ref": "C",
      "alt": "T",
      "effect": "stop_gained",
      "transcript": "ENST00000352241.9",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MITF",
          "gene_hgnc_id": 7105,
          "hgvs_c": "c.1084C>T",
          "hgvs_p": "p.Arg362*",
          "transcript": "NM_001354604.2",
          "protein_id": "NP_001341533.1",
          "transcript_support_level": null,
          "aa_start": 362,
          "aa_end": null,
          "aa_length": 526,
          "cds_start": 1084,
          "cds_end": null,
          "cds_length": 1581,
          "cdna_start": 1218,
          "cdna_end": null,
          "cdna_length": 4799,
          "mane_select": "ENST00000352241.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MITF",
          "gene_hgnc_id": 7105,
          "hgvs_c": "c.1084C>T",
          "hgvs_p": "p.Arg362*",
          "transcript": "ENST00000352241.9",
          "protein_id": "ENSP00000295600.8",
          "transcript_support_level": 1,
          "aa_start": 362,
          "aa_end": null,
          "aa_length": 526,
          "cds_start": 1084,
          "cds_end": null,
          "cds_length": 1581,
          "cdna_start": 1218,
          "cdna_end": null,
          "cdna_length": 4799,
          "mane_select": "NM_001354604.2",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MITF",
          "gene_hgnc_id": 7105,
          "hgvs_c": "c.763C>T",
          "hgvs_p": "p.Arg255*",
          "transcript": "NM_000248.4",
          "protein_id": "NP_000239.1",
          "transcript_support_level": null,
          "aa_start": 255,
          "aa_end": null,
          "aa_length": 419,
          "cds_start": 763,
          "cds_end": null,
          "cds_length": 1260,
          "cdna_start": 894,
          "cdna_end": null,
          "cdna_length": 4475,
          "mane_select": null,
          "mane_plus": "ENST00000394351.9",
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MITF",
          "gene_hgnc_id": 7105,
          "hgvs_c": "c.763C>T",
          "hgvs_p": "p.Arg255*",
          "transcript": "ENST00000394351.9",
          "protein_id": "ENSP00000377880.3",
          "transcript_support_level": 1,
          "aa_start": 255,
          "aa_end": null,
          "aa_length": 419,
          "cds_start": 763,
          "cds_end": null,
          "cds_length": 1260,
          "cdna_start": 894,
          "cdna_end": null,
          "cdna_length": 4475,
          "mane_select": null,
          "mane_plus": "NM_000248.4",
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MITF",
          "gene_hgnc_id": 7105,
          "hgvs_c": "c.745C>T",
          "hgvs_p": "p.Arg249*",
          "transcript": "ENST00000314557.10",
          "protein_id": "ENSP00000324246.6",
          "transcript_support_level": 1,
          "aa_start": 249,
          "aa_end": null,
          "aa_length": 413,
          "cds_start": 745,
          "cds_end": null,
          "cds_length": 1242,
          "cdna_start": 881,
          "cdna_end": null,
          "cdna_length": 1798,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MITF",
          "gene_hgnc_id": 7105,
          "hgvs_c": "c.577C>T",
          "hgvs_p": "p.Arg193*",
          "transcript": "ENST00000531774.1",
          "protein_id": "ENSP00000435909.1",
          "transcript_support_level": 1,
          "aa_start": 193,
          "aa_end": null,
          "aa_length": 357,
          "cds_start": 577,
          "cds_end": null,
          "cds_length": 1074,
          "cdna_start": 577,
          "cdna_end": null,
          "cdna_length": 1074,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MITF",
          "gene_hgnc_id": 7105,
          "hgvs_c": "c.1081C>T",
          "hgvs_p": "p.Arg361*",
          "transcript": "NM_001354605.2",
          "protein_id": "NP_001341534.1",
          "transcript_support_level": null,
          "aa_start": 361,
          "aa_end": null,
          "aa_length": 525,
          "cds_start": 1081,
          "cds_end": null,
          "cds_length": 1578,
          "cdna_start": 1215,
          "cdna_end": null,
          "cdna_length": 4796,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MITF",
          "gene_hgnc_id": 7105,
          "hgvs_c": "c.1066C>T",
          "hgvs_p": "p.Arg356*",
          "transcript": "NM_198159.3",
          "protein_id": "NP_937802.1",
          "transcript_support_level": null,
          "aa_start": 356,
          "aa_end": null,
          "aa_length": 520,
          "cds_start": 1066,
          "cds_end": null,
          "cds_length": 1563,
          "cdna_start": 1200,
          "cdna_end": null,
          "cdna_length": 4781,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MITF",
          "gene_hgnc_id": 7105,
          "hgvs_c": "c.1066C>T",
          "hgvs_p": "p.Arg356*",
          "transcript": "ENST00000642352.1",
          "protein_id": "ENSP00000494105.1",
          "transcript_support_level": null,
          "aa_start": 356,
          "aa_end": null,
          "aa_length": 520,
          "cds_start": 1066,
          "cds_end": null,
          "cds_length": 1563,
          "cdna_start": 1182,
          "cdna_end": null,
          "cdna_length": 4767,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MITF",
          "gene_hgnc_id": 7105,
          "hgvs_c": "c.1063C>T",
          "hgvs_p": "p.Arg355*",
          "transcript": "NM_001354606.2",
          "protein_id": "NP_001341535.1",
          "transcript_support_level": null,
          "aa_start": 355,
          "aa_end": null,
          "aa_length": 519,
          "cds_start": 1063,
          "cds_end": null,
          "cds_length": 1560,
          "cdna_start": 1197,
          "cdna_end": null,
          "cdna_length": 4778,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MITF",
          "gene_hgnc_id": 7105,
          "hgvs_c": "c.1063C>T",
          "hgvs_p": "p.Arg355*",
          "transcript": "NM_006722.3",
          "protein_id": "NP_006713.1",
          "transcript_support_level": null,
          "aa_start": 355,
          "aa_end": null,
          "aa_length": 519,
          "cds_start": 1063,
          "cds_end": null,
          "cds_length": 1560,
          "cdna_start": 1094,
          "cdna_end": null,
          "cdna_length": 4675,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MITF",
          "gene_hgnc_id": 7105,
          "hgvs_c": "c.1063C>T",
          "hgvs_p": "p.Arg355*",
          "transcript": "ENST00000448226.9",
          "protein_id": "ENSP00000391803.3",
          "transcript_support_level": 5,
          "aa_start": 355,
          "aa_end": null,
          "aa_length": 519,
          "cds_start": 1063,
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          "cdna_start": 1094,
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          "cdna_length": 4675,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MITF",
          "gene_hgnc_id": 7105,
          "hgvs_c": "c.1018C>T",
          "hgvs_p": "p.Arg340*",
          "transcript": "NM_198177.3",
          "protein_id": "NP_937820.1",
          "transcript_support_level": null,
          "aa_start": 340,
          "aa_end": null,
          "aa_length": 504,
          "cds_start": 1018,
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          "cds_length": 1515,
          "cdna_start": 1085,
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          "cdna_length": 4666,
          "mane_select": null,
          "mane_plus": null,
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          "feature": null
        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MITF",
          "gene_hgnc_id": 7105,
          "hgvs_c": "c.1018C>T",
          "hgvs_p": "p.Arg340*",
          "transcript": "ENST00000314589.11",
          "protein_id": "ENSP00000324443.5",
          "transcript_support_level": 2,
          "aa_start": 340,
          "aa_end": null,
          "aa_length": 504,
          "cds_start": 1018,
          "cds_end": null,
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          "cdna_start": 1085,
          "cdna_end": null,
          "cdna_length": 4666,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 10,
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          "exon_count": 11,
          "intron_rank": null,
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          "gene_symbol": "MITF",
          "gene_hgnc_id": 7105,
          "hgvs_c": "c.1015C>T",
          "hgvs_p": "p.Arg339*",
          "transcript": "NM_001354607.2",
          "protein_id": "NP_001341536.1",
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          "cdna_start": 1329,
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          "feature": null
        },
        {
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MITF",
          "gene_hgnc_id": 7105,
          "hgvs_c": "c.1015C>T",
          "hgvs_p": "p.Arg339*",
          "transcript": "ENST00000687384.1",
          "protein_id": "ENSP00000510225.1",
          "transcript_support_level": null,
          "aa_start": 339,
          "aa_end": null,
          "aa_length": 503,
          "cds_start": 1015,
          "cds_end": null,
          "cds_length": 1512,
          "cdna_start": 1337,
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MITF",
          "gene_hgnc_id": 7105,
          "hgvs_c": "c.991C>T",
          "hgvs_p": "p.Arg331*",
          "transcript": "ENST00000693031.1",
          "protein_id": "ENSP00000509845.1",
          "transcript_support_level": null,
          "aa_start": 331,
          "aa_end": null,
          "aa_length": 495,
          "cds_start": 991,
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          "cds_length": 1488,
          "cdna_start": 1129,
          "cdna_end": null,
          "cdna_length": 2011,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 9,
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          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MITF",
          "gene_hgnc_id": 7105,
          "hgvs_c": "c.910C>T",
          "hgvs_p": "p.Arg304*",
          "transcript": "NM_001184967.2",
          "protein_id": "NP_001171896.1",
          "transcript_support_level": null,
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          "cdna_start": 1151,
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        },
        {
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          "protein_coding": true,
          "strand": true,
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          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
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          "gene_symbol": "MITF",
          "gene_hgnc_id": 7105,
          "hgvs_c": "c.910C>T",
          "hgvs_p": "p.Arg304*",
          "transcript": "NM_001354608.2",
          "protein_id": "NP_001341537.1",
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          "cdna_length": 4820,
          "mane_select": null,
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        },
        {
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
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          "gene_symbol": "MITF",
          "gene_hgnc_id": 7105,
          "hgvs_c": "c.910C>T",
          "hgvs_p": "p.Arg304*",
          "transcript": "ENST00000472437.5",
          "protein_id": "ENSP00000418845.1",
          "transcript_support_level": 2,
          "aa_start": 304,
          "aa_end": null,
          "aa_length": 468,
          "cds_start": 910,
          "cds_end": null,
          "cds_length": 1407,
          "cdna_start": 1099,
          "cdna_end": null,
          "cdna_length": 1995,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MITF",
          "gene_hgnc_id": 7105,
          "hgvs_c": "c.745C>T",
          "hgvs_p": "p.Arg249*",
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        },
        {
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          "gene_symbol": "MITF",
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          "transcript": "ENST00000478490.5",
          "protein_id": "ENSP00000433487.1",
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        }
      ],
      "gene_symbol": "MITF",
      "gene_hgnc_id": 7105,
      "dbsnp": "rs1057517966",
      "frequency_reference_population": 6.8408343e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84083e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.6299999952316284,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.63,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 1.931,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 18,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PVS1,PM2,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 18,
          "benign_score": 0,
          "pathogenic_score": 18,
          "criteria": [
            "PVS1",
            "PM2",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000352241.9",
          "gene_symbol": "MITF",
          "hgnc_id": 7105,
          "effects": [
            "stop_gained"
          ],
          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.1084C>T",
          "hgvs_p": "p.Arg362*"
        }
      ],
      "clinvar_disease": " 8, cutaneous malignant, susceptibility to,Congenital sensorineural hearing impairment,MITF-related disorder,Melanoma,Tietz syndrome,Waardenburg syndrome type 2A,not provided",
      "clinvar_classification": "Pathogenic/Likely pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:3 LP:2",
      "phenotype_combined": "not provided|Waardenburg syndrome type 2A|MITF-related disorder|Congenital sensorineural hearing impairment|Waardenburg syndrome type 2A;Tietz syndrome;Melanoma, cutaneous malignant, susceptibility to, 8",
      "pathogenicity_classification_combined": "Pathogenic/Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}