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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-9840258-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=9840258&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 9840258,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_173659.5",
      "consequences": [
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "c.626A>G",
          "hgvs_p": "p.Lys209Arg",
          "transcript": "NM_173659.5",
          "protein_id": "NP_775930.3",
          "transcript_support_level": null,
          "aa_start": 209,
          "aa_end": null,
          "aa_length": 343,
          "cds_start": 626,
          "cds_end": null,
          "cds_length": 1032,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000383820.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_173659.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "c.626A>G",
          "hgvs_p": "p.Lys209Arg",
          "transcript": "ENST00000383820.10",
          "protein_id": "ENSP00000373331.6",
          "transcript_support_level": 1,
          "aa_start": 209,
          "aa_end": null,
          "aa_length": 343,
          "cds_start": 626,
          "cds_end": null,
          "cds_length": 1032,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_173659.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000383820.10"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "c.581A>G",
          "hgvs_p": "p.Lys194Arg",
          "transcript": "ENST00000433535.7",
          "protein_id": "ENSP00000398921.3",
          "transcript_support_level": 1,
          "aa_start": 194,
          "aa_end": null,
          "aa_length": 328,
          "cds_start": 581,
          "cds_end": null,
          "cds_length": 987,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000433535.7"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "c.752A>G",
          "hgvs_p": "p.Lys251Arg",
          "transcript": "ENST00000923702.1",
          "protein_id": "ENSP00000593761.1",
          "transcript_support_level": null,
          "aa_start": 251,
          "aa_end": null,
          "aa_length": 385,
          "cds_start": 752,
          "cds_end": null,
          "cds_length": 1158,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000923702.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "c.626A>G",
          "hgvs_p": "p.Lys209Arg",
          "transcript": "ENST00000954644.1",
          "protein_id": "ENSP00000624703.1",
          "transcript_support_level": null,
          "aa_start": 209,
          "aa_end": null,
          "aa_length": 355,
          "cds_start": 626,
          "cds_end": null,
          "cds_length": 1068,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000954644.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "c.626A>G",
          "hgvs_p": "p.Lys209Arg",
          "transcript": "ENST00000923701.1",
          "protein_id": "ENSP00000593760.1",
          "transcript_support_level": null,
          "aa_start": 209,
          "aa_end": null,
          "aa_length": 343,
          "cds_start": 626,
          "cds_end": null,
          "cds_length": 1032,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000923701.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "c.620A>G",
          "hgvs_p": "p.Lys207Arg",
          "transcript": "ENST00000870303.1",
          "protein_id": "ENSP00000540362.1",
          "transcript_support_level": null,
          "aa_start": 207,
          "aa_end": null,
          "aa_length": 341,
          "cds_start": 620,
          "cds_end": null,
          "cds_length": 1026,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000870303.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "c.617A>G",
          "hgvs_p": "p.Lys206Arg",
          "transcript": "ENST00000923703.1",
          "protein_id": "ENSP00000593762.1",
          "transcript_support_level": null,
          "aa_start": 206,
          "aa_end": null,
          "aa_length": 340,
          "cds_start": 617,
          "cds_end": null,
          "cds_length": 1023,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000923703.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "c.596A>G",
          "hgvs_p": "p.Lys199Arg",
          "transcript": "ENST00000954645.1",
          "protein_id": "ENSP00000624704.1",
          "transcript_support_level": null,
          "aa_start": 199,
          "aa_end": null,
          "aa_length": 333,
          "cds_start": 596,
          "cds_end": null,
          "cds_length": 1002,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000954645.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "c.581A>G",
          "hgvs_p": "p.Lys194Arg",
          "transcript": "NM_001142547.3",
          "protein_id": "NP_001136019.2",
          "transcript_support_level": null,
          "aa_start": 194,
          "aa_end": null,
          "aa_length": 328,
          "cds_start": 581,
          "cds_end": null,
          "cds_length": 987,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001142547.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "c.518A>G",
          "hgvs_p": "p.Lys173Arg",
          "transcript": "ENST00000870304.1",
          "protein_id": "ENSP00000540363.1",
          "transcript_support_level": null,
          "aa_start": 173,
          "aa_end": null,
          "aa_length": 307,
          "cds_start": 518,
          "cds_end": null,
          "cds_length": 924,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000870304.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "c.626A>G",
          "hgvs_p": "p.Lys209Arg",
          "transcript": "ENST00000418713.6",
          "protein_id": "ENSP00000415616.2",
          "transcript_support_level": 2,
          "aa_start": 209,
          "aa_end": null,
          "aa_length": 242,
          "cds_start": 626,
          "cds_end": null,
          "cds_length": 729,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000418713.6"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "c.626A>G",
          "hgvs_p": "p.Lys209Arg",
          "transcript": "NM_001351737.2",
          "protein_id": "NP_001338666.2",
          "transcript_support_level": null,
          "aa_start": 209,
          "aa_end": null,
          "aa_length": 236,
          "cds_start": 626,
          "cds_end": null,
          "cds_length": 711,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001351737.2"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "c.554A>G",
          "hgvs_p": "p.Lys185Arg",
          "transcript": "NM_001351736.3",
          "protein_id": "NP_001338665.1",
          "transcript_support_level": null,
          "aa_start": 185,
          "aa_end": null,
          "aa_length": 212,
          "cds_start": 554,
          "cds_end": null,
          "cds_length": 639,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001351736.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "c.554A>G",
          "hgvs_p": "p.Lys185Arg",
          "transcript": "ENST00000424438.5",
          "protein_id": "ENSP00000408693.1",
          "transcript_support_level": 2,
          "aa_start": 185,
          "aa_end": null,
          "aa_length": 212,
          "cds_start": 554,
          "cds_end": null,
          "cds_length": 639,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000424438.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "c.134A>G",
          "hgvs_p": "p.Lys45Arg",
          "transcript": "ENST00000423108.5",
          "protein_id": "ENSP00000405054.1",
          "transcript_support_level": 2,
          "aa_start": 45,
          "aa_end": null,
          "aa_length": 143,
          "cds_start": 134,
          "cds_end": null,
          "cds_length": 432,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000423108.5"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "c.654A>G",
          "hgvs_p": "p.Gln218Gln",
          "transcript": "NM_001351738.2",
          "protein_id": "NP_001338667.2",
          "transcript_support_level": null,
          "aa_start": 218,
          "aa_end": null,
          "aa_length": 281,
          "cds_start": 654,
          "cds_end": null,
          "cds_length": 846,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001351738.2"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "c.648A>G",
          "hgvs_p": "p.Gln216Gln",
          "transcript": "ENST00000427174.5",
          "protein_id": "ENSP00000400397.1",
          "transcript_support_level": 5,
          "aa_start": 216,
          "aa_end": null,
          "aa_length": 279,
          "cds_start": 648,
          "cds_end": null,
          "cds_length": 840,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000427174.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TTLL3",
          "gene_hgnc_id": 24483,
          "hgvs_c": "c.918+10863T>C",
          "hgvs_p": null,
          "transcript": "ENST00000455274.5",
          "protein_id": "ENSP00000409632.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 326,
          "cds_start": null,
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          "cds_length": 981,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000455274.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": 6,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "c.576+274A>G",
          "hgvs_p": null,
          "transcript": "ENST00000923705.1",
          "protein_id": "ENSP00000593764.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 255,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 768,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000923705.1"
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
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          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
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          "hgvs_c": "c.246+1725A>G",
          "hgvs_p": null,
          "transcript": "ENST00000923704.1",
          "protein_id": "ENSP00000593763.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000923704.1"
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "c.201+1725A>G",
          "hgvs_p": null,
          "transcript": "ENST00000923706.1",
          "protein_id": "ENSP00000593765.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 130,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 393,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000923706.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "n.609A>G",
          "hgvs_p": null,
          "transcript": "ENST00000464783.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000464783.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "n.2056A>G",
          "hgvs_p": null,
          "transcript": "ENST00000475470.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000475470.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPUSD3",
          "gene_hgnc_id": 28437,
          "hgvs_c": "n.2134A>G",
          "hgvs_p": null,
          "transcript": "ENST00000484134.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000484134.5"
        }
      ],
      "gene_symbol": "RPUSD3",
      "gene_hgnc_id": 28437,
      "dbsnp": "rs553409406",
      "frequency_reference_population": 0.000009912055,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 16,
      "gnomad_exomes_af": 0.00000547238,
      "gnomad_genomes_af": 0.0000525245,
      "gnomad_exomes_ac": 8,
      "gnomad_genomes_ac": 8,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.16446301341056824,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.709,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.0873,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.43,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.902,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_173659.5",
          "gene_symbol": "RPUSD3",
          "hgnc_id": 28437,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.626A>G",
          "hgvs_p": "p.Lys209Arg"
        },
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000455274.5",
          "gene_symbol": "TTLL3",
          "hgnc_id": 24483,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.918+10863T>C",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}