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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-112541153-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=112541153&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 112541153,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_018392.5",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZGRF1",
          "gene_hgnc_id": 25654,
          "hgvs_c": "c.5714G>A",
          "hgvs_p": "p.Arg1905Gln",
          "transcript": "NM_018392.5",
          "protein_id": "NP_060862.3",
          "transcript_support_level": null,
          "aa_start": 1905,
          "aa_end": null,
          "aa_length": 2104,
          "cds_start": 5714,
          "cds_end": null,
          "cds_length": 6315,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000505019.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_018392.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZGRF1",
          "gene_hgnc_id": 25654,
          "hgvs_c": "c.5714G>A",
          "hgvs_p": "p.Arg1905Gln",
          "transcript": "ENST00000505019.6",
          "protein_id": "ENSP00000424737.1",
          "transcript_support_level": 5,
          "aa_start": 1905,
          "aa_end": null,
          "aa_length": 2104,
          "cds_start": 5714,
          "cds_end": null,
          "cds_length": 6315,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_018392.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000505019.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZGRF1",
          "gene_hgnc_id": 25654,
          "hgvs_c": "c.5714G>A",
          "hgvs_p": "p.Arg1905Gln",
          "transcript": "ENST00000445203.6",
          "protein_id": "ENSP00000390505.3",
          "transcript_support_level": 5,
          "aa_start": 1905,
          "aa_end": null,
          "aa_length": 2104,
          "cds_start": 5714,
          "cds_end": null,
          "cds_length": 6315,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000445203.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZGRF1",
          "gene_hgnc_id": 25654,
          "hgvs_c": "c.5540G>A",
          "hgvs_p": "p.Arg1847Gln",
          "transcript": "NM_001350397.2",
          "protein_id": "NP_001337326.1",
          "transcript_support_level": null,
          "aa_start": 1847,
          "aa_end": null,
          "aa_length": 2046,
          "cds_start": 5540,
          "cds_end": null,
          "cds_length": 6141,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001350397.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZGRF1",
          "gene_hgnc_id": 25654,
          "hgvs_c": "c.5540G>A",
          "hgvs_p": "p.Arg1847Gln",
          "transcript": "ENST00000925931.1",
          "protein_id": "ENSP00000595990.1",
          "transcript_support_level": null,
          "aa_start": 1847,
          "aa_end": null,
          "aa_length": 2046,
          "cds_start": 5540,
          "cds_end": null,
          "cds_length": 6141,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925931.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZGRF1",
          "gene_hgnc_id": 25654,
          "hgvs_c": "c.5489G>A",
          "hgvs_p": "p.Arg1830Gln",
          "transcript": "ENST00000925932.1",
          "protein_id": "ENSP00000595991.1",
          "transcript_support_level": null,
          "aa_start": 1830,
          "aa_end": null,
          "aa_length": 2029,
          "cds_start": 5489,
          "cds_end": null,
          "cds_length": 6090,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925932.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZGRF1",
          "gene_hgnc_id": 25654,
          "hgvs_c": "c.5480G>A",
          "hgvs_p": "p.Arg1827Gln",
          "transcript": "ENST00000925930.1",
          "protein_id": "ENSP00000595989.1",
          "transcript_support_level": null,
          "aa_start": 1827,
          "aa_end": null,
          "aa_length": 2026,
          "cds_start": 5480,
          "cds_end": null,
          "cds_length": 6081,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925930.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZGRF1",
          "gene_hgnc_id": 25654,
          "hgvs_c": "c.5480G>A",
          "hgvs_p": "p.Arg1827Gln",
          "transcript": "ENST00000925934.1",
          "protein_id": "ENSP00000595993.1",
          "transcript_support_level": null,
          "aa_start": 1827,
          "aa_end": null,
          "aa_length": 2026,
          "cds_start": 5480,
          "cds_end": null,
          "cds_length": 6081,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925934.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZGRF1",
          "gene_hgnc_id": 25654,
          "hgvs_c": "c.5456G>A",
          "hgvs_p": "p.Arg1819Gln",
          "transcript": "ENST00000925933.1",
          "protein_id": "ENSP00000595992.1",
          "transcript_support_level": null,
          "aa_start": 1819,
          "aa_end": null,
          "aa_length": 2018,
          "cds_start": 5456,
          "cds_end": null,
          "cds_length": 6057,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925933.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZGRF1",
          "gene_hgnc_id": 25654,
          "hgvs_c": "c.5714G>A",
          "hgvs_p": "p.Arg1905Gln",
          "transcript": "XM_005263115.5",
          "protein_id": "XP_005263172.1",
          "transcript_support_level": null,
          "aa_start": 1905,
          "aa_end": null,
          "aa_length": 2104,
          "cds_start": 5714,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZGRF1",
          "gene_hgnc_id": 25654,
          "hgvs_c": "c.5663G>A",
          "hgvs_p": "p.Arg1888Gln",
          "transcript": "XM_011532091.3",
          "protein_id": "XP_011530393.1",
          "transcript_support_level": null,
          "aa_start": 1888,
          "aa_end": null,
          "aa_length": 2087,
          "cds_start": 5663,
          "cds_end": null,
          "cds_length": 6264,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_011532091.3"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
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          "gene_symbol": "ZGRF1",
          "gene_hgnc_id": 25654,
          "hgvs_c": "c.5663G>A",
          "hgvs_p": "p.Arg1888Gln",
          "transcript": "XM_047415909.1",
          "protein_id": "XP_047271865.1",
          "transcript_support_level": null,
          "aa_start": 1888,
          "aa_end": null,
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          "cds_start": 5663,
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          "cdna_start": null,
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        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 24,
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          "intron_rank": null,
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          "gene_symbol": "ZGRF1",
          "gene_hgnc_id": 25654,
          "hgvs_c": "c.5654G>A",
          "hgvs_p": "p.Arg1885Gln",
          "transcript": "XM_011532092.3",
          "protein_id": "XP_011530394.1",
          "transcript_support_level": null,
          "aa_start": 1885,
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          "cds_start": 5654,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZGRF1",
          "gene_hgnc_id": 25654,
          "hgvs_c": "c.5654G>A",
          "hgvs_p": "p.Arg1885Gln",
          "transcript": "XM_017008369.3",
          "protein_id": "XP_016863858.1",
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          "cds_start": 5654,
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        {
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          "intron_rank": null,
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          "gene_symbol": "ZGRF1",
          "gene_hgnc_id": 25654,
          "hgvs_c": "c.5603G>A",
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          "transcript": "XM_047415910.1",
          "protein_id": "XP_047271866.1",
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          "aa_end": null,
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          "cds_start": 5603,
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          "consequences": [
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          ],
          "exon_rank": 24,
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          "exon_count": 27,
          "intron_rank": null,
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          "gene_symbol": "ZGRF1",
          "gene_hgnc_id": 25654,
          "hgvs_c": "c.5540G>A",
          "hgvs_p": "p.Arg1847Gln",
          "transcript": "XM_047415911.1",
          "protein_id": "XP_047271867.1",
          "transcript_support_level": null,
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        {
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          "strand": false,
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          ],
          "exon_rank": 23,
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          "exon_count": 26,
          "intron_rank": null,
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          "gene_symbol": "ZGRF1",
          "gene_hgnc_id": 25654,
          "hgvs_c": "c.5489G>A",
          "hgvs_p": "p.Arg1830Gln",
          "transcript": "XM_017008370.2",
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        {
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        },
        {
          "aa_ref": "R",
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          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
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          "gene_symbol": "ZGRF1",
          "gene_hgnc_id": 25654,
          "hgvs_c": "c.5480G>A",
          "hgvs_p": "p.Arg1827Gln",
          "transcript": "XM_047415914.1",
          "protein_id": "XP_047271870.1",
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          "intron_rank_end": null,
          "gene_symbol": "NEUROG2-AS1",
          "gene_hgnc_id": 40656,
          "hgvs_c": "n.634-5632C>T",
          "hgvs_p": null,
          "transcript": "ENST00000754795.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000754795.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "NEUROG2-AS1",
          "gene_hgnc_id": 40656,
          "hgvs_c": "n.457-5632C>T",
          "hgvs_p": null,
          "transcript": "ENST00000754796.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000754796.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "NEUROG2-AS1",
          "gene_hgnc_id": 40656,
          "hgvs_c": "n.513-5632C>T",
          "hgvs_p": null,
          "transcript": "NR_161159.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_161159.1"
        }
      ],
      "gene_symbol": "ZGRF1",
      "gene_hgnc_id": 25654,
      "dbsnp": "rs574383993",
      "frequency_reference_population": 0.000030424919,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 49,
      "gnomad_exomes_af": 0.0000308575,
      "gnomad_genomes_af": 0.0000262802,
      "gnomad_exomes_ac": 45,
      "gnomad_genomes_ac": 4,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.3829326033592224,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.557,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.2699,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.11,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 5.384,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_018392.5",
          "gene_symbol": "ZGRF1",
          "hgnc_id": 25654,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.5714G>A",
          "hgvs_p": "p.Arg1905Gln"
        },
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000504009.2",
          "gene_symbol": "NEUROG2-AS1",
          "hgnc_id": 40656,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.462-5632C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}