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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-118723540-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=118723540&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 118723540,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_001318066.2",
      "consequences": [
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC24D",
          "gene_hgnc_id": 10706,
          "hgvs_c": "c.3074A>G",
          "hgvs_p": "p.Lys1025Arg",
          "transcript": "NM_014822.4",
          "protein_id": "NP_055637.2",
          "transcript_support_level": null,
          "aa_start": 1025,
          "aa_end": null,
          "aa_length": 1032,
          "cds_start": 3074,
          "cds_end": null,
          "cds_length": 3099,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000280551.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_014822.4"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC24D",
          "gene_hgnc_id": 10706,
          "hgvs_c": "c.3074A>G",
          "hgvs_p": "p.Lys1025Arg",
          "transcript": "ENST00000280551.11",
          "protein_id": "ENSP00000280551.6",
          "transcript_support_level": 1,
          "aa_start": 1025,
          "aa_end": null,
          "aa_length": 1032,
          "cds_start": 3074,
          "cds_end": null,
          "cds_length": 3099,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_014822.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000280551.11"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC24D",
          "gene_hgnc_id": 10706,
          "hgvs_c": "c.1967A>G",
          "hgvs_p": "p.Lys656Arg",
          "transcript": "ENST00000511481.5",
          "protein_id": "ENSP00000425491.1",
          "transcript_support_level": 1,
          "aa_start": 656,
          "aa_end": null,
          "aa_length": 663,
          "cds_start": 1967,
          "cds_end": null,
          "cds_length": 1992,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000511481.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC24D",
          "gene_hgnc_id": 10706,
          "hgvs_c": "c.3077A>G",
          "hgvs_p": "p.Lys1026Arg",
          "transcript": "NM_001318066.2",
          "protein_id": "NP_001304995.1",
          "transcript_support_level": null,
          "aa_start": 1026,
          "aa_end": null,
          "aa_length": 1033,
          "cds_start": 3077,
          "cds_end": null,
          "cds_length": 3102,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001318066.2"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC24D",
          "gene_hgnc_id": 10706,
          "hgvs_c": "c.3074A>G",
          "hgvs_p": "p.Lys1025Arg",
          "transcript": "ENST00000924655.1",
          "protein_id": "ENSP00000594714.1",
          "transcript_support_level": null,
          "aa_start": 1025,
          "aa_end": null,
          "aa_length": 1032,
          "cds_start": 3074,
          "cds_end": null,
          "cds_length": 3099,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000924655.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC24D",
          "gene_hgnc_id": 10706,
          "hgvs_c": "c.3074A>G",
          "hgvs_p": "p.Lys1025Arg",
          "transcript": "ENST00000924658.1",
          "protein_id": "ENSP00000594717.1",
          "transcript_support_level": null,
          "aa_start": 1025,
          "aa_end": null,
          "aa_length": 1032,
          "cds_start": 3074,
          "cds_end": null,
          "cds_length": 3099,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000924658.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC24D",
          "gene_hgnc_id": 10706,
          "hgvs_c": "c.3071A>G",
          "hgvs_p": "p.Lys1024Arg",
          "transcript": "ENST00000924656.1",
          "protein_id": "ENSP00000594715.1",
          "transcript_support_level": null,
          "aa_start": 1024,
          "aa_end": null,
          "aa_length": 1031,
          "cds_start": 3071,
          "cds_end": null,
          "cds_length": 3096,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000924656.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC24D",
          "gene_hgnc_id": 10706,
          "hgvs_c": "c.2960A>G",
          "hgvs_p": "p.Lys987Arg",
          "transcript": "ENST00000899696.1",
          "protein_id": "ENSP00000569755.1",
          "transcript_support_level": null,
          "aa_start": 987,
          "aa_end": null,
          "aa_length": 994,
          "cds_start": 2960,
          "cds_end": null,
          "cds_length": 2985,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000899696.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC24D",
          "gene_hgnc_id": 10706,
          "hgvs_c": "c.2957A>G",
          "hgvs_p": "p.Lys986Arg",
          "transcript": "ENST00000899695.1",
          "protein_id": "ENSP00000569754.1",
          "transcript_support_level": null,
          "aa_start": 986,
          "aa_end": null,
          "aa_length": 993,
          "cds_start": 2957,
          "cds_end": null,
          "cds_length": 2982,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000899695.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC24D",
          "gene_hgnc_id": 10706,
          "hgvs_c": "c.2786A>G",
          "hgvs_p": "p.Lys929Arg",
          "transcript": "ENST00000924657.1",
          "protein_id": "ENSP00000594716.1",
          "transcript_support_level": null,
          "aa_start": 929,
          "aa_end": null,
          "aa_length": 936,
          "cds_start": 2786,
          "cds_end": null,
          "cds_length": 2811,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000924657.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC24D",
          "gene_hgnc_id": 10706,
          "hgvs_c": "n.403A>G",
          "hgvs_p": null,
          "transcript": "ENST00000502830.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000502830.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC24D",
          "gene_hgnc_id": 10706,
          "hgvs_c": "n.3205A>G",
          "hgvs_p": null,
          "transcript": "ENST00000505134.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000505134.5"
        }
      ],
      "gene_symbol": "SEC24D",
      "gene_hgnc_id": 10706,
      "dbsnp": "rs193163880",
      "frequency_reference_population": 0.000010536442,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 17,
      "gnomad_exomes_af": 0.00000889713,
      "gnomad_genomes_af": 0.0000262636,
      "gnomad_exomes_ac": 13,
      "gnomad_genomes_ac": 4,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.06696674227714539,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.102,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.075,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.57,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.536,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001318066.2",
          "gene_symbol": "SEC24D",
          "hgnc_id": 10706,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.3077A>G",
          "hgvs_p": "p.Lys1026Arg"
        }
      ],
      "clinvar_disease": "Inborn genetic diseases,not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:2",
      "phenotype_combined": "not provided|Inborn genetic diseases",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}