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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-119501172-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=119501172&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 119501172,
      "ref": "C",
      "alt": "G",
      "effect": "missense_variant,splice_region_variant",
      "transcript": "NM_001083.4",
      "consequences": [
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE5A",
          "gene_hgnc_id": 8784,
          "hgvs_c": "c.2488G>C",
          "hgvs_p": "p.Glu830Gln",
          "transcript": "NM_001083.4",
          "protein_id": "NP_001074.2",
          "transcript_support_level": null,
          "aa_start": 830,
          "aa_end": null,
          "aa_length": 875,
          "cds_start": 2488,
          "cds_end": null,
          "cds_length": 2628,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000354960.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001083.4"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE5A",
          "gene_hgnc_id": 8784,
          "hgvs_c": "c.2488G>C",
          "hgvs_p": "p.Glu830Gln",
          "transcript": "ENST00000354960.8",
          "protein_id": "ENSP00000347046.3",
          "transcript_support_level": 1,
          "aa_start": 830,
          "aa_end": null,
          "aa_length": 875,
          "cds_start": 2488,
          "cds_end": null,
          "cds_length": 2628,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001083.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000354960.8"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE5A",
          "gene_hgnc_id": 8784,
          "hgvs_c": "c.2362G>C",
          "hgvs_p": "p.Glu788Gln",
          "transcript": "ENST00000264805.9",
          "protein_id": "ENSP00000264805.5",
          "transcript_support_level": 1,
          "aa_start": 788,
          "aa_end": null,
          "aa_length": 833,
          "cds_start": 2362,
          "cds_end": null,
          "cds_length": 2502,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000264805.9"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE5A",
          "gene_hgnc_id": 8784,
          "hgvs_c": "c.541G>C",
          "hgvs_p": "p.Glu181Gln",
          "transcript": "ENST00000503412.1",
          "protein_id": "ENSP00000425810.1",
          "transcript_support_level": 3,
          "aa_start": 181,
          "aa_end": null,
          "aa_length": 184,
          "cds_start": 541,
          "cds_end": null,
          "cds_length": 555,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000503412.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE5A",
          "gene_hgnc_id": 8784,
          "hgvs_c": "c.2485G>C",
          "hgvs_p": "p.Glu829Gln",
          "transcript": "ENST00000925607.1",
          "protein_id": "ENSP00000595666.1",
          "transcript_support_level": null,
          "aa_start": 829,
          "aa_end": null,
          "aa_length": 874,
          "cds_start": 2485,
          "cds_end": null,
          "cds_length": 2625,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925607.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE5A",
          "gene_hgnc_id": 8784,
          "hgvs_c": "c.2362G>C",
          "hgvs_p": "p.Glu788Gln",
          "transcript": "NM_033430.3",
          "protein_id": "NP_236914.2",
          "transcript_support_level": null,
          "aa_start": 788,
          "aa_end": null,
          "aa_length": 833,
          "cds_start": 2362,
          "cds_end": null,
          "cds_length": 2502,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_033430.3"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE5A",
          "gene_hgnc_id": 8784,
          "hgvs_c": "c.2341G>C",
          "hgvs_p": "p.Glu781Gln",
          "transcript": "ENST00000925608.1",
          "protein_id": "ENSP00000595667.1",
          "transcript_support_level": null,
          "aa_start": 781,
          "aa_end": null,
          "aa_length": 826,
          "cds_start": 2341,
          "cds_end": null,
          "cds_length": 2481,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925608.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE5A",
          "gene_hgnc_id": 8784,
          "hgvs_c": "c.2332G>C",
          "hgvs_p": "p.Glu778Gln",
          "transcript": "NM_033437.4",
          "protein_id": "NP_246273.2",
          "transcript_support_level": null,
          "aa_start": 778,
          "aa_end": null,
          "aa_length": 823,
          "cds_start": 2332,
          "cds_end": null,
          "cds_length": 2472,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_033437.4"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE5A",
          "gene_hgnc_id": 8784,
          "hgvs_c": "c.2332G>C",
          "hgvs_p": "p.Glu778Gln",
          "transcript": "ENST00000394439.5",
          "protein_id": "ENSP00000377957.1",
          "transcript_support_level": 5,
          "aa_start": 778,
          "aa_end": null,
          "aa_length": 823,
          "cds_start": 2332,
          "cds_end": null,
          "cds_length": 2472,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000394439.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000291203",
          "gene_hgnc_id": null,
          "hgvs_c": "n.364+3269C>G",
          "hgvs_p": null,
          "transcript": "ENST00000498873.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000498873.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": 8,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000291203",
          "gene_hgnc_id": null,
          "hgvs_c": "n.931+6649C>G",
          "hgvs_p": null,
          "transcript": "ENST00000508519.6",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000508519.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000291203",
          "gene_hgnc_id": null,
          "hgvs_c": "n.191+3269C>G",
          "hgvs_p": null,
          "transcript": "ENST00000510844.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000510844.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": 9,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000291203",
          "gene_hgnc_id": null,
          "hgvs_c": "n.1046+3269C>G",
          "hgvs_p": null,
          "transcript": "ENST00000685525.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000685525.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": 8,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000291203",
          "gene_hgnc_id": null,
          "hgvs_c": "n.953+3269C>G",
          "hgvs_p": null,
          "transcript": "ENST00000685974.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
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          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000685974.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": 7,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000291203",
          "gene_hgnc_id": null,
          "hgvs_c": "n.910+6649C>G",
          "hgvs_p": null,
          "transcript": "ENST00000686202.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000686202.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": 8,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000291203",
          "gene_hgnc_id": null,
          "hgvs_c": "n.1018+3269C>G",
          "hgvs_p": null,
          "transcript": "ENST00000688315.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000688315.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": 8,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000291203",
          "gene_hgnc_id": null,
          "hgvs_c": "n.1007+3269C>G",
          "hgvs_p": null,
          "transcript": "ENST00000688480.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
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          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000688480.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": 8,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000291203",
          "gene_hgnc_id": null,
          "hgvs_c": "n.997+3269C>G",
          "hgvs_p": null,
          "transcript": "ENST00000690731.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000690731.2"
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": 8,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000291203",
          "gene_hgnc_id": null,
          "hgvs_c": "n.939+3269C>G",
          "hgvs_p": null,
          "transcript": "ENST00000692409.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
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          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000692409.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": 9,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000291203",
          "gene_hgnc_id": null,
          "hgvs_c": "n.1037+3269C>G",
          "hgvs_p": null,
          "transcript": "ENST00000692642.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000692642.2"
        },
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          "gene_hgnc_id": null,
          "hgvs_c": "n.1072+3269C>G",
          "hgvs_p": null,
          "transcript": "ENST00000776145.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000776145.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": 8,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000291203",
          "gene_hgnc_id": null,
          "hgvs_c": "n.953+3265C>G",
          "hgvs_p": null,
          "transcript": "ENST00000776146.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000776146.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 8,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000291203",
          "gene_hgnc_id": null,
          "hgvs_c": "n.979+3269C>G",
          "hgvs_p": null,
          "transcript": "ENST00000776147.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000776147.1"
        }
      ],
      "gene_symbol": "PDE5A",
      "gene_hgnc_id": 8784,
      "dbsnp": "rs199573045",
      "frequency_reference_population": 0.000054611195,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 87,
      "gnomad_exomes_af": 0.0000326199,
      "gnomad_genomes_af": 0.000262743,
      "gnomad_exomes_ac": 47,
      "gnomad_genomes_ac": 40,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.3514193296432495,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.414000004529953,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "dbscSNV1_RF",
      "revel_score": 0.387,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.1591,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.14,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 5.607,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": 0.225841464971702,
      "dbscsnv_ada_prediction": "Benign",
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -1,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4",
      "acmg_by_gene": [
        {
          "score": -1,
          "benign_score": 1,
          "pathogenic_score": 0,
          "criteria": [
            "BP4"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001083.4",
          "gene_symbol": "PDE5A",
          "hgnc_id": 8784,
          "effects": [
            "missense_variant",
            "splice_region_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.2488G>C",
          "hgvs_p": "p.Glu830Gln"
        },
        {
          "score": -1,
          "benign_score": 1,
          "pathogenic_score": 0,
          "criteria": [
            "BP4"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000508519.6",
          "gene_symbol": "ENSG00000291203",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.931+6649C>G",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}