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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-125487373-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=125487373&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 125487373,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "ENST00000394329.9",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAT4",
          "gene_hgnc_id": 23109,
          "hgvs_c": "c.12851C>T",
          "hgvs_p": "p.Ser4284Phe",
          "transcript": "NM_001291303.3",
          "protein_id": "NP_001278232.1",
          "transcript_support_level": null,
          "aa_start": 4284,
          "aa_end": null,
          "aa_length": 4983,
          "cds_start": 12851,
          "cds_end": null,
          "cds_length": 14952,
          "cdna_start": 13886,
          "cdna_end": null,
          "cdna_length": 17151,
          "mane_select": "ENST00000394329.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAT4",
          "gene_hgnc_id": 23109,
          "hgvs_c": "c.12851C>T",
          "hgvs_p": "p.Ser4284Phe",
          "transcript": "ENST00000394329.9",
          "protein_id": "ENSP00000377862.4",
          "transcript_support_level": 5,
          "aa_start": 4284,
          "aa_end": null,
          "aa_length": 4983,
          "cds_start": 12851,
          "cds_end": null,
          "cds_length": 14952,
          "cdna_start": 13886,
          "cdna_end": null,
          "cdna_length": 17151,
          "mane_select": "NM_001291303.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAT4",
          "gene_hgnc_id": 23109,
          "hgvs_c": "c.7568C>T",
          "hgvs_p": "p.Ser2523Phe",
          "transcript": "ENST00000335110.5",
          "protein_id": "ENSP00000335169.5",
          "transcript_support_level": 1,
          "aa_start": 2523,
          "aa_end": null,
          "aa_length": 3222,
          "cds_start": 7568,
          "cds_end": null,
          "cds_length": 9669,
          "cdna_start": 7568,
          "cdna_end": null,
          "cdna_length": 9669,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAT4",
          "gene_hgnc_id": 23109,
          "hgvs_c": "c.12851C>T",
          "hgvs_p": "p.Ser4284Phe",
          "transcript": "NM_001438396.1",
          "protein_id": "NP_001425325.1",
          "transcript_support_level": null,
          "aa_start": 4284,
          "aa_end": null,
          "aa_length": 4983,
          "cds_start": 12851,
          "cds_end": null,
          "cds_length": 14952,
          "cdna_start": 14308,
          "cdna_end": null,
          "cdna_length": 17573,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAT4",
          "gene_hgnc_id": 23109,
          "hgvs_c": "c.12851C>T",
          "hgvs_p": "p.Ser4284Phe",
          "transcript": "NM_001291285.3",
          "protein_id": "NP_001278214.1",
          "transcript_support_level": null,
          "aa_start": 4284,
          "aa_end": null,
          "aa_length": 4982,
          "cds_start": 12851,
          "cds_end": null,
          "cds_length": 14949,
          "cdna_start": 13886,
          "cdna_end": null,
          "cdna_length": 17148,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAT4",
          "gene_hgnc_id": 23109,
          "hgvs_c": "c.12851C>T",
          "hgvs_p": "p.Ser4284Phe",
          "transcript": "NM_001438397.1",
          "protein_id": "NP_001425326.1",
          "transcript_support_level": null,
          "aa_start": 4284,
          "aa_end": null,
          "aa_length": 4982,
          "cds_start": 12851,
          "cds_end": null,
          "cds_length": 14949,
          "cdna_start": 14308,
          "cdna_end": null,
          "cdna_length": 17570,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAT4",
          "gene_hgnc_id": 23109,
          "hgvs_c": "c.12845C>T",
          "hgvs_p": "p.Ser4282Phe",
          "transcript": "NM_024582.6",
          "protein_id": "NP_078858.4",
          "transcript_support_level": null,
          "aa_start": 4282,
          "aa_end": null,
          "aa_length": 4981,
          "cds_start": 12845,
          "cds_end": null,
          "cds_length": 14946,
          "cdna_start": 13880,
          "cdna_end": null,
          "cdna_length": 17145,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAT4",
          "gene_hgnc_id": 23109,
          "hgvs_c": "c.7622C>T",
          "hgvs_p": "p.Ser2541Phe",
          "transcript": "NM_001437895.1",
          "protein_id": "NP_001424824.1",
          "transcript_support_level": null,
          "aa_start": 2541,
          "aa_end": null,
          "aa_length": 3240,
          "cds_start": 7622,
          "cds_end": null,
          "cds_length": 9723,
          "cdna_start": 8699,
          "cdna_end": null,
          "cdna_length": 11964,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAT4",
          "gene_hgnc_id": 23109,
          "hgvs_c": "c.7622C>T",
          "hgvs_p": "p.Ser2541Phe",
          "transcript": "ENST00000674496.2",
          "protein_id": "ENSP00000501473.2",
          "transcript_support_level": null,
          "aa_start": 2541,
          "aa_end": null,
          "aa_length": 3240,
          "cds_start": 7622,
          "cds_end": null,
          "cds_length": 9723,
          "cdna_start": 8736,
          "cdna_end": null,
          "cdna_length": 12001,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "FAT4",
      "gene_hgnc_id": 23109,
      "dbsnp": "rs199682210",
      "frequency_reference_population": 0.0001903005,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 307,
      "gnomad_exomes_af": 0.000197792,
      "gnomad_genomes_af": 0.000118337,
      "gnomad_exomes_ac": 289,
      "gnomad_genomes_ac": 18,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8773025274276733,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.20000000298023224,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.838,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9622,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.26,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.2,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PP3_Moderate,BS1_Supporting",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PP3_Moderate",
            "BS1_Supporting"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000394329.9",
          "gene_symbol": "FAT4",
          "hgnc_id": 23109,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.12851C>T",
          "hgvs_p": "p.Ser4284Phe"
        }
      ],
      "clinvar_disease": "FAT4-related disorder,Hennekam lymphangiectasia-lymphedema syndrome 2,Van Maldergem syndrome 2,not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:4",
      "phenotype_combined": "Hennekam lymphangiectasia-lymphedema syndrome 2|not provided|Hennekam lymphangiectasia-lymphedema syndrome 2;Van Maldergem syndrome 2|FAT4-related disorder",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}