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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-127886668-TTAAAG-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=127886668&ref=TTAAAG&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 127886668,
      "ref": "TTAAAG",
      "alt": "T",
      "effect": "frameshift_variant",
      "transcript": "ENST00000270861.10",
      "consequences": [
        {
          "aa_ref": "FKE",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLK4",
          "gene_hgnc_id": 11397,
          "hgvs_c": "c.1299_1303delTAAAG",
          "hgvs_p": "p.Phe433fs",
          "transcript": "NM_014264.5",
          "protein_id": "NP_055079.3",
          "transcript_support_level": null,
          "aa_start": 433,
          "aa_end": null,
          "aa_length": 970,
          "cds_start": 1299,
          "cds_end": null,
          "cds_length": 2913,
          "cdna_start": 1541,
          "cdna_end": null,
          "cdna_length": 3838,
          "mane_select": "ENST00000270861.10",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "FKE",
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLK4",
          "gene_hgnc_id": 11397,
          "hgvs_c": "c.1299_1303delTAAAG",
          "hgvs_p": "p.Phe433fs",
          "transcript": "ENST00000270861.10",
          "protein_id": "ENSP00000270861.5",
          "transcript_support_level": 1,
          "aa_start": 433,
          "aa_end": null,
          "aa_length": 970,
          "cds_start": 1299,
          "cds_end": null,
          "cds_length": 2913,
          "cdna_start": 1541,
          "cdna_end": null,
          "cdna_length": 3838,
          "mane_select": "NM_014264.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "FKE",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLK4",
          "gene_hgnc_id": 11397,
          "hgvs_c": "c.1302_1306delTAAAG",
          "hgvs_p": "p.Phe434fs",
          "transcript": "NM_001441357.1",
          "protein_id": "NP_001428286.1",
          "transcript_support_level": null,
          "aa_start": 434,
          "aa_end": null,
          "aa_length": 971,
          "cds_start": 1302,
          "cds_end": null,
          "cds_length": 2916,
          "cdna_start": 1343,
          "cdna_end": null,
          "cdna_length": 3640,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "FKE",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLK4",
          "gene_hgnc_id": 11397,
          "hgvs_c": "c.1203_1207delTAAAG",
          "hgvs_p": "p.Phe401fs",
          "transcript": "NM_001190799.2",
          "protein_id": "NP_001177728.1",
          "transcript_support_level": null,
          "aa_start": 401,
          "aa_end": null,
          "aa_length": 938,
          "cds_start": 1203,
          "cds_end": null,
          "cds_length": 2817,
          "cdna_start": 1445,
          "cdna_end": null,
          "cdna_length": 3742,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "FKE",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLK4",
          "gene_hgnc_id": 11397,
          "hgvs_c": "c.1203_1207delTAAAG",
          "hgvs_p": "p.Phe401fs",
          "transcript": "ENST00000513090.5",
          "protein_id": "ENSP00000427554.1",
          "transcript_support_level": 2,
          "aa_start": 401,
          "aa_end": null,
          "aa_length": 938,
          "cds_start": 1203,
          "cds_end": null,
          "cds_length": 2817,
          "cdna_start": 1411,
          "cdna_end": null,
          "cdna_length": 3123,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "FKE",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLK4",
          "gene_hgnc_id": 11397,
          "hgvs_c": "c.1176_1180delTAAAG",
          "hgvs_p": "p.Phe392fs",
          "transcript": "NM_001190801.2",
          "protein_id": "NP_001177730.1",
          "transcript_support_level": null,
          "aa_start": 392,
          "aa_end": null,
          "aa_length": 929,
          "cds_start": 1176,
          "cds_end": null,
          "cds_length": 2790,
          "cdna_start": 1408,
          "cdna_end": null,
          "cdna_length": 3705,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "FKE",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLK4",
          "gene_hgnc_id": 11397,
          "hgvs_c": "c.1176_1180delTAAAG",
          "hgvs_p": "p.Phe392fs",
          "transcript": "ENST00000514379.5",
          "protein_id": "ENSP00000423582.1",
          "transcript_support_level": 2,
          "aa_start": 392,
          "aa_end": null,
          "aa_length": 929,
          "cds_start": 1176,
          "cds_end": null,
          "cds_length": 2790,
          "cdna_start": 1408,
          "cdna_end": null,
          "cdna_length": 3060,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "FKE",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLK4",
          "gene_hgnc_id": 11397,
          "hgvs_c": "c.1158_1162delTAAAG",
          "hgvs_p": "p.Phe386fs",
          "transcript": "NM_001441360.1",
          "protein_id": "NP_001428289.1",
          "transcript_support_level": null,
          "aa_start": 386,
          "aa_end": null,
          "aa_length": 923,
          "cds_start": 1158,
          "cds_end": null,
          "cds_length": 2772,
          "cdna_start": 1537,
          "cdna_end": null,
          "cdna_length": 3834,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "FKE",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLK4",
          "gene_hgnc_id": 11397,
          "hgvs_c": "c.1299_1303delTAAAG",
          "hgvs_p": "p.Phe433fs",
          "transcript": "NM_001441362.1",
          "protein_id": "NP_001428291.1",
          "transcript_support_level": null,
          "aa_start": 433,
          "aa_end": null,
          "aa_length": 897,
          "cds_start": 1299,
          "cds_end": null,
          "cds_length": 2694,
          "cdna_start": 1541,
          "cdna_end": null,
          "cdna_length": 3619,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "FKE",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLK4",
          "gene_hgnc_id": 11397,
          "hgvs_c": "c.1299_1303delTAAAG",
          "hgvs_p": "p.Phe433fs",
          "transcript": "ENST00000515069.5",
          "protein_id": "ENSP00000421774.1",
          "transcript_support_level": 5,
          "aa_start": 433,
          "aa_end": null,
          "aa_length": 892,
          "cds_start": 1299,
          "cds_end": null,
          "cds_length": 2679,
          "cdna_start": 1525,
          "cdna_end": null,
          "cdna_length": 3431,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "FKE",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLK4",
          "gene_hgnc_id": 11397,
          "hgvs_c": "c.1029_1033delTAAAG",
          "hgvs_p": "p.Phe343fs",
          "transcript": "NM_001441368.1",
          "protein_id": "NP_001428297.1",
          "transcript_support_level": null,
          "aa_start": 343,
          "aa_end": null,
          "aa_length": 880,
          "cds_start": 1029,
          "cds_end": null,
          "cds_length": 2643,
          "cdna_start": 1522,
          "cdna_end": null,
          "cdna_length": 3819,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "FKE",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLK4",
          "gene_hgnc_id": 11397,
          "hgvs_c": "c.678_682delTAAAG",
          "hgvs_p": "p.Phe226fs",
          "transcript": "NM_001441371.1",
          "protein_id": "NP_001428300.1",
          "transcript_support_level": null,
          "aa_start": 226,
          "aa_end": null,
          "aa_length": 763,
          "cds_start": 678,
          "cds_end": null,
          "cds_length": 2292,
          "cdna_start": 920,
          "cdna_end": null,
          "cdna_length": 3217,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": 5,
          "intron_rank_end": null,
          "gene_symbol": "PLK4",
          "gene_hgnc_id": 11397,
          "hgvs_c": "c.1259+43_1259+47delTAAAG",
          "hgvs_p": null,
          "transcript": "NM_001441358.1",
          "protein_id": "NP_001428287.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 937,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2814,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3538,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": 5,
          "intron_rank_end": null,
          "gene_symbol": "PLK4",
          "gene_hgnc_id": 11397,
          "hgvs_c": "c.1256+43_1256+47delTAAAG",
          "hgvs_p": null,
          "transcript": "NM_001441359.1",
          "protein_id": "NP_001428288.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 936,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2811,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3736,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": 5,
          "intron_rank_end": null,
          "gene_symbol": "PLK4",
          "gene_hgnc_id": 11397,
          "hgvs_c": "c.1256+43_1256+47delTAAAG",
          "hgvs_p": null,
          "transcript": "ENST00000507249.5",
          "protein_id": "ENSP00000423412.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 909,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2730,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3195,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "PLK4",
          "gene_hgnc_id": 11397,
          "hgvs_c": "c.1160+43_1160+47delTAAAG",
          "hgvs_p": null,
          "transcript": "NM_001441361.1",
          "protein_id": "NP_001428290.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 904,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2715,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3640,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": 5,
          "intron_rank_end": null,
          "gene_symbol": "PLK4",
          "gene_hgnc_id": 11397,
          "hgvs_c": "c.1133+43_1133+47delTAAAG",
          "hgvs_p": null,
          "transcript": "NM_001441366.1",
          "protein_id": "NP_001428295.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 895,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2688,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3799,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": 5,
          "intron_rank_end": null,
          "gene_symbol": "PLK4",
          "gene_hgnc_id": 11397,
          "hgvs_c": "c.1115+43_1115+47delTAAAG",
          "hgvs_p": null,
          "transcript": "NM_001441367.1",
          "protein_id": "NP_001428296.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 889,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2670,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3732,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": 5,
          "intron_rank_end": null,
          "gene_symbol": "PLK4",
          "gene_hgnc_id": 11397,
          "hgvs_c": "c.1256+43_1256+47delTAAAG",
          "hgvs_p": null,
          "transcript": "NM_001441369.1",
          "protein_id": "NP_001428298.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 863,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2592,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3517,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "PLK4",
      "gene_hgnc_id": 11397,
      "dbsnp": "rs724159996",
      "frequency_reference_population": 0.000014257518,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 23,
      "gnomad_exomes_af": 0.00000958241,
      "gnomad_genomes_af": 0.0000591428,
      "gnomad_exomes_ac": 14,
      "gnomad_genomes_ac": 9,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": null,
      "computational_prediction_selected": null,
      "computational_source_selected": null,
      "splice_score_selected": null,
      "splice_prediction_selected": null,
      "splice_source_selected": null,
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": null,
      "bayesdelnoaf_prediction": null,
      "phylop100way_score": 3.852,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": null,
      "spliceai_max_prediction": null,
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 11,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PVS1,PM2,PP5",
      "acmg_by_gene": [
        {
          "score": 11,
          "benign_score": 0,
          "pathogenic_score": 11,
          "criteria": [
            "PVS1",
            "PM2",
            "PP5"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000270861.10",
          "gene_symbol": "PLK4",
          "hgnc_id": 11397,
          "effects": [
            "frameshift_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1299_1303delTAAAG",
          "hgvs_p": "p.Phe433fs"
        }
      ],
      "clinvar_disease": "Microcephaly and chorioretinopathy 2,not provided",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "P:2 US:1",
      "phenotype_combined": "Microcephaly and chorioretinopathy 2|not provided",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}