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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-140395849-T-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=140395849&ref=T&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 140395849,
      "ref": "T",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_004362.3",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLGN",
          "gene_hgnc_id": 2060,
          "hgvs_c": "c.1119A>T",
          "hgvs_p": "p.Arg373Ser",
          "transcript": "NM_004362.3",
          "protein_id": "NP_004353.1",
          "transcript_support_level": null,
          "aa_start": 373,
          "aa_end": null,
          "aa_length": 610,
          "cds_start": 1119,
          "cds_end": null,
          "cds_length": 1833,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000325617.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_004362.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLGN",
          "gene_hgnc_id": 2060,
          "hgvs_c": "c.1119A>T",
          "hgvs_p": "p.Arg373Ser",
          "transcript": "ENST00000325617.10",
          "protein_id": "ENSP00000326699.5",
          "transcript_support_level": 1,
          "aa_start": 373,
          "aa_end": null,
          "aa_length": 610,
          "cds_start": 1119,
          "cds_end": null,
          "cds_length": 1833,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_004362.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000325617.10"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLGN",
          "gene_hgnc_id": 2060,
          "hgvs_c": "c.1119A>T",
          "hgvs_p": "p.Arg373Ser",
          "transcript": "ENST00000414773.5",
          "protein_id": "ENSP00000392782.1",
          "transcript_support_level": 1,
          "aa_start": 373,
          "aa_end": null,
          "aa_length": 610,
          "cds_start": 1119,
          "cds_end": null,
          "cds_length": 1833,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000414773.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLGN",
          "gene_hgnc_id": 2060,
          "hgvs_c": "c.1119A>T",
          "hgvs_p": "p.Arg373Ser",
          "transcript": "NM_001130675.2",
          "protein_id": "NP_001124147.1",
          "transcript_support_level": null,
          "aa_start": 373,
          "aa_end": null,
          "aa_length": 610,
          "cds_start": 1119,
          "cds_end": null,
          "cds_length": 1833,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001130675.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLGN",
          "gene_hgnc_id": 2060,
          "hgvs_c": "c.1119A>T",
          "hgvs_p": "p.Arg373Ser",
          "transcript": "ENST00000897460.1",
          "protein_id": "ENSP00000567519.1",
          "transcript_support_level": null,
          "aa_start": 373,
          "aa_end": null,
          "aa_length": 610,
          "cds_start": 1119,
          "cds_end": null,
          "cds_length": 1833,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000897460.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLGN",
          "gene_hgnc_id": 2060,
          "hgvs_c": "c.1119A>T",
          "hgvs_p": "p.Arg373Ser",
          "transcript": "ENST00000931054.1",
          "protein_id": "ENSP00000601113.1",
          "transcript_support_level": null,
          "aa_start": 373,
          "aa_end": null,
          "aa_length": 610,
          "cds_start": 1119,
          "cds_end": null,
          "cds_length": 1833,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000931054.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLGN",
          "gene_hgnc_id": 2060,
          "hgvs_c": "c.1119A>T",
          "hgvs_p": "p.Arg373Ser",
          "transcript": "ENST00000931055.1",
          "protein_id": "ENSP00000601114.1",
          "transcript_support_level": null,
          "aa_start": 373,
          "aa_end": null,
          "aa_length": 610,
          "cds_start": 1119,
          "cds_end": null,
          "cds_length": 1833,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000931055.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLGN",
          "gene_hgnc_id": 2060,
          "hgvs_c": "c.1119A>T",
          "hgvs_p": "p.Arg373Ser",
          "transcript": "ENST00000963782.1",
          "protein_id": "ENSP00000633841.1",
          "transcript_support_level": null,
          "aa_start": 373,
          "aa_end": null,
          "aa_length": 609,
          "cds_start": 1119,
          "cds_end": null,
          "cds_length": 1830,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000963782.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLGN",
          "gene_hgnc_id": 2060,
          "hgvs_c": "c.1119A>T",
          "hgvs_p": "p.Arg373Ser",
          "transcript": "ENST00000963785.1",
          "protein_id": "ENSP00000633844.1",
          "transcript_support_level": null,
          "aa_start": 373,
          "aa_end": null,
          "aa_length": 609,
          "cds_start": 1119,
          "cds_end": null,
          "cds_length": 1830,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000963785.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLGN",
          "gene_hgnc_id": 2060,
          "hgvs_c": "c.951A>T",
          "hgvs_p": "p.Arg317Ser",
          "transcript": "ENST00000963784.1",
          "protein_id": "ENSP00000633843.1",
          "transcript_support_level": null,
          "aa_start": 317,
          "aa_end": null,
          "aa_length": 554,
          "cds_start": 951,
          "cds_end": null,
          "cds_length": 1665,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000963784.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLGN",
          "gene_hgnc_id": 2060,
          "hgvs_c": "c.654A>T",
          "hgvs_p": "p.Arg218Ser",
          "transcript": "ENST00000963783.1",
          "protein_id": "ENSP00000633842.1",
          "transcript_support_level": null,
          "aa_start": 218,
          "aa_end": null,
          "aa_length": 455,
          "cds_start": 654,
          "cds_end": null,
          "cds_length": 1368,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000963783.1"
        }
      ],
      "gene_symbol": "CLGN",
      "gene_hgnc_id": 2060,
      "dbsnp": "rs377332621",
      "frequency_reference_population": 0.000055653418,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 86,
      "gnomad_exomes_af": 0.0000559865,
      "gnomad_genomes_af": 0.0000526025,
      "gnomad_exomes_ac": 78,
      "gnomad_genomes_ac": 8,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.07336226105690002,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.092,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.2204,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.58,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.014,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_004362.3",
          "gene_symbol": "CLGN",
          "hgnc_id": 2060,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1119A>T",
          "hgvs_p": "p.Arg373Ser"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}