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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-144116906-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=144116906&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 144116906,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_002099.8",
      "consequences": [
        {
          "aa_ref": "G",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GYPA",
          "gene_hgnc_id": 4702,
          "hgvs_c": "c.305G>A",
          "hgvs_p": "p.Gly102Asp",
          "transcript": "NM_002099.8",
          "protein_id": "NP_002090.4",
          "transcript_support_level": null,
          "aa_start": 102,
          "aa_end": null,
          "aa_length": 150,
          "cds_start": 305,
          "cds_end": null,
          "cds_length": 453,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000641688.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_002099.8"
        },
        {
          "aa_ref": "G",
          "aa_alt": "D",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GYPA",
          "gene_hgnc_id": 4702,
          "hgvs_c": "c.305G>A",
          "hgvs_p": "p.Gly102Asp",
          "transcript": "ENST00000641688.3",
          "protein_id": "ENSP00000493142.2",
          "transcript_support_level": null,
          "aa_start": 102,
          "aa_end": null,
          "aa_length": 150,
          "cds_start": 305,
          "cds_end": null,
          "cds_length": 453,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_002099.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000641688.3"
        },
        {
          "aa_ref": "G",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GYPA",
          "gene_hgnc_id": 4702,
          "hgvs_c": "c.305G>A",
          "hgvs_p": "p.Gly102Asp",
          "transcript": "ENST00000360771.8",
          "protein_id": "ENSP00000354003.4",
          "transcript_support_level": 1,
          "aa_start": 102,
          "aa_end": null,
          "aa_length": 150,
          "cds_start": 305,
          "cds_end": null,
          "cds_length": 453,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000360771.8"
        },
        {
          "aa_ref": "G",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GYPA",
          "gene_hgnc_id": 4702,
          "hgvs_c": "c.299G>A",
          "hgvs_p": "p.Gly100Asp",
          "transcript": "ENST00000535709.6",
          "protein_id": "ENSP00000445398.2",
          "transcript_support_level": 1,
          "aa_start": 100,
          "aa_end": null,
          "aa_length": 148,
          "cds_start": 299,
          "cds_end": null,
          "cds_length": 447,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000535709.6"
        },
        {
          "aa_ref": "G",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GYPA",
          "gene_hgnc_id": 4702,
          "hgvs_c": "c.266G>A",
          "hgvs_p": "p.Gly89Asp",
          "transcript": "ENST00000512064.5",
          "protein_id": "ENSP00000426130.1",
          "transcript_support_level": 1,
          "aa_start": 89,
          "aa_end": null,
          "aa_length": 137,
          "cds_start": 266,
          "cds_end": null,
          "cds_length": 414,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000512064.5"
        },
        {
          "aa_ref": "G",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GYPA",
          "gene_hgnc_id": 4702,
          "hgvs_c": "c.209G>A",
          "hgvs_p": "p.Gly70Asp",
          "transcript": "ENST00000504786.5",
          "protein_id": "ENSP00000425549.1",
          "transcript_support_level": 1,
          "aa_start": 70,
          "aa_end": null,
          "aa_length": 118,
          "cds_start": 209,
          "cds_end": null,
          "cds_length": 357,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000504786.5"
        },
        {
          "aa_ref": "G",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GYPA",
          "gene_hgnc_id": 4702,
          "hgvs_c": "c.206G>A",
          "hgvs_p": "p.Gly69Asp",
          "transcript": "ENST00000324022.14",
          "protein_id": "ENSP00000324483.10",
          "transcript_support_level": 1,
          "aa_start": 69,
          "aa_end": null,
          "aa_length": 117,
          "cds_start": 206,
          "cds_end": null,
          "cds_length": 354,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000324022.14"
        },
        {
          "aa_ref": "G",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GYPA",
          "gene_hgnc_id": 4702,
          "hgvs_c": "c.170G>A",
          "hgvs_p": "p.Gly57Asp",
          "transcript": "ENST00000503627.2",
          "protein_id": "ENSP00000421243.1",
          "transcript_support_level": 1,
          "aa_start": 57,
          "aa_end": null,
          "aa_length": 105,
          "cds_start": 170,
          "cds_end": null,
          "cds_length": 318,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000503627.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GYPA",
          "gene_hgnc_id": 4702,
          "hgvs_c": "c.110G>A",
          "hgvs_p": "p.Gly37Asp",
          "transcript": "ENST00000512789.5",
          "protein_id": "ENSP00000425193.1",
          "transcript_support_level": 1,
          "aa_start": 37,
          "aa_end": null,
          "aa_length": 85,
          "cds_start": 110,
          "cds_end": null,
          "cds_length": 258,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000512789.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GYPA",
          "gene_hgnc_id": 4702,
          "hgvs_c": "n.*259G>A",
          "hgvs_p": null,
          "transcript": "ENST00000508337.1",
          "protein_id": "ENSP00000425719.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000508337.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GYPA",
          "gene_hgnc_id": 4702,
          "hgvs_c": "n.*259G>A",
          "hgvs_p": null,
          "transcript": "ENST00000508337.1",
          "protein_id": "ENSP00000425719.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000508337.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GYPA",
          "gene_hgnc_id": 4702,
          "hgvs_c": "c.299G>A",
          "hgvs_p": "p.Gly100Asp",
          "transcript": "ENST00000616983.4",
          "protein_id": "ENSP00000478329.1",
          "transcript_support_level": 5,
          "aa_start": 100,
          "aa_end": null,
          "aa_length": 148,
          "cds_start": 299,
          "cds_end": null,
          "cds_length": 447,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000616983.4"
        },
        {
          "aa_ref": "G",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GYPA",
          "gene_hgnc_id": 4702,
          "hgvs_c": "c.305G>A",
          "hgvs_p": "p.Gly102Asp",
          "transcript": "NM_001438046.1",
          "protein_id": "NP_001424975.1",
          "transcript_support_level": null,
          "aa_start": 102,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 305,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001438046.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GYPA",
          "gene_hgnc_id": 4702,
          "hgvs_c": "c.305G>A",
          "hgvs_p": "p.Gly102Asp",
          "transcript": "ENST00000642295.1",
          "protein_id": "ENSP00000496079.1",
          "transcript_support_level": null,
          "aa_start": 102,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 305,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000642295.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GYPA",
          "gene_hgnc_id": 4702,
          "hgvs_c": "c.266G>A",
          "hgvs_p": "p.Gly89Asp",
          "transcript": "NM_001308187.2",
          "protein_id": "NP_001295116.1",
          "transcript_support_level": null,
          "aa_start": 89,
          "aa_end": null,
          "aa_length": 137,
          "cds_start": 266,
          "cds_end": null,
          "cds_length": 414,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001308187.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GYPA",
          "gene_hgnc_id": 4702,
          "hgvs_c": "c.227G>A",
          "hgvs_p": "p.Gly76Asp",
          "transcript": "NM_001438627.1",
          "protein_id": "NP_001425556.1",
          "transcript_support_level": null,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 124,
          "cds_start": 227,
          "cds_end": null,
          "cds_length": 375,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001438627.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GYPA",
          "gene_hgnc_id": 4702,
          "hgvs_c": "c.227G>A",
          "hgvs_p": "p.Gly76Asp",
          "transcript": "ENST00000642713.1",
          "protein_id": "ENSP00000494092.1",
          "transcript_support_level": null,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 124,
          "cds_start": 227,
          "cds_end": null,
          "cds_length": 375,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000642713.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GYPA",
          "gene_hgnc_id": 4702,
          "hgvs_c": "c.227G>A",
          "hgvs_p": "p.Gly76Asp",
          "transcript": "ENST00000646447.1",
          "protein_id": "ENSP00000495922.1",
          "transcript_support_level": null,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 124,
          "cds_start": 227,
          "cds_end": null,
          "cds_length": 375,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000646447.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GYPA",
          "gene_hgnc_id": 4702,
          "hgvs_c": "c.206G>A",
          "hgvs_p": "p.Gly69Asp",
          "transcript": "NM_001308190.2",
          "protein_id": "NP_001295119.1",
          "transcript_support_level": null,
          "aa_start": 69,
          "aa_end": null,
          "aa_length": 117,
          "cds_start": 206,
          "cds_end": null,
          "cds_length": 354,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001308190.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GYPA",
          "gene_hgnc_id": 4702,
          "hgvs_c": "c.206G>A",
          "hgvs_p": "p.Gly69Asp",
          "transcript": "NM_001438625.1",
          "protein_id": "NP_001425554.1",
          "transcript_support_level": null,
          "aa_start": 69,
          "aa_end": null,
          "aa_length": 112,
          "cds_start": 206,
          "cds_end": null,
          "cds_length": 339,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001438625.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "feature": "XR_002959803.2"
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      ],
      "gene_symbol": "GYPA",
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      "dbsnp": "rs574776481",
      "frequency_reference_population": 0.000010653832,
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      "allele_count_reference_population": 17,
      "gnomad_exomes_af": 0.0000103919,
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      "gnomad_exomes_homalt": 0,
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      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8267751932144165,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.278,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.9628,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.26,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -1.158,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
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      "acmg_score": 3,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3",
      "acmg_by_gene": [
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
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            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_002099.8",
          "gene_symbol": "GYPA",
          "hgnc_id": 4702,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "BG",
          "hgvs_c": "c.305G>A",
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        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
            "PM2",
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000650526.1",
          "gene_symbol": "ENSG00000285783",
          "hgnc_id": null,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.1261G>A",
          "hgvs_p": null
        },
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
            "PM2",
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000641428.1",
          "gene_symbol": "GUSBP5",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.643-1334C>T",
          "hgvs_p": null
        },
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
            "PM2",
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "XR_002959803.2",
          "gene_symbol": "LOC105377460",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.5270+1677C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}