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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-153704643-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=153704643&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 153704643,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_003264.5",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TLR2",
          "gene_hgnc_id": 11848,
          "hgvs_c": "c.1736G>A",
          "hgvs_p": "p.Arg579His",
          "transcript": "NM_001318789.2",
          "protein_id": "NP_001305718.1",
          "transcript_support_level": null,
          "aa_start": 579,
          "aa_end": null,
          "aa_length": 784,
          "cds_start": 1736,
          "cds_end": null,
          "cds_length": 2355,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000642700.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001318789.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TLR2",
          "gene_hgnc_id": 11848,
          "hgvs_c": "c.1736G>A",
          "hgvs_p": "p.Arg579His",
          "transcript": "ENST00000642700.2",
          "protein_id": "ENSP00000494425.1",
          "transcript_support_level": null,
          "aa_start": 579,
          "aa_end": null,
          "aa_length": 784,
          "cds_start": 1736,
          "cds_end": null,
          "cds_length": 2355,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001318789.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000642700.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TLR2",
          "gene_hgnc_id": 11848,
          "hgvs_c": "c.1736G>A",
          "hgvs_p": "p.Arg579His",
          "transcript": "NM_001318787.2",
          "protein_id": "NP_001305716.1",
          "transcript_support_level": null,
          "aa_start": 579,
          "aa_end": null,
          "aa_length": 784,
          "cds_start": 1736,
          "cds_end": null,
          "cds_length": 2355,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001318787.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TLR2",
          "gene_hgnc_id": 11848,
          "hgvs_c": "c.1736G>A",
          "hgvs_p": "p.Arg579His",
          "transcript": "NM_001318790.2",
          "protein_id": "NP_001305719.1",
          "transcript_support_level": null,
          "aa_start": 579,
          "aa_end": null,
          "aa_length": 784,
          "cds_start": 1736,
          "cds_end": null,
          "cds_length": 2355,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001318790.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TLR2",
          "gene_hgnc_id": 11848,
          "hgvs_c": "c.1736G>A",
          "hgvs_p": "p.Arg579His",
          "transcript": "NM_001318791.2",
          "protein_id": "NP_001305720.1",
          "transcript_support_level": null,
          "aa_start": 579,
          "aa_end": null,
          "aa_length": 784,
          "cds_start": 1736,
          "cds_end": null,
          "cds_length": 2355,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001318791.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TLR2",
          "gene_hgnc_id": 11848,
          "hgvs_c": "c.1736G>A",
          "hgvs_p": "p.Arg579His",
          "transcript": "NM_001318793.2",
          "protein_id": "NP_001305722.1",
          "transcript_support_level": null,
          "aa_start": 579,
          "aa_end": null,
          "aa_length": 784,
          "cds_start": 1736,
          "cds_end": null,
          "cds_length": 2355,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001318793.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TLR2",
          "gene_hgnc_id": 11848,
          "hgvs_c": "c.1736G>A",
          "hgvs_p": "p.Arg579His",
          "transcript": "NM_001318795.2",
          "protein_id": "NP_001305724.1",
          "transcript_support_level": null,
          "aa_start": 579,
          "aa_end": null,
          "aa_length": 784,
          "cds_start": 1736,
          "cds_end": null,
          "cds_length": 2355,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001318795.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TLR2",
          "gene_hgnc_id": 11848,
          "hgvs_c": "c.1736G>A",
          "hgvs_p": "p.Arg579His",
          "transcript": "NM_001318796.2",
          "protein_id": "NP_001305725.1",
          "transcript_support_level": null,
          "aa_start": 579,
          "aa_end": null,
          "aa_length": 784,
          "cds_start": 1736,
          "cds_end": null,
          "cds_length": 2355,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001318796.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TLR2",
          "gene_hgnc_id": 11848,
          "hgvs_c": "c.1736G>A",
          "hgvs_p": "p.Arg579His",
          "transcript": "NM_003264.5",
          "protein_id": "NP_003255.2",
          "transcript_support_level": null,
          "aa_start": 579,
          "aa_end": null,
          "aa_length": 784,
          "cds_start": 1736,
          "cds_end": null,
          "cds_length": 2355,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_003264.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TLR2",
          "gene_hgnc_id": 11848,
          "hgvs_c": "c.1736G>A",
          "hgvs_p": "p.Arg579His",
          "transcript": "ENST00000260010.7",
          "protein_id": "ENSP00000260010.6",
          "transcript_support_level": 6,
          "aa_start": 579,
          "aa_end": null,
          "aa_length": 784,
          "cds_start": 1736,
          "cds_end": null,
          "cds_length": 2355,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000260010.7"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TLR2",
          "gene_hgnc_id": 11848,
          "hgvs_c": "c.1736G>A",
          "hgvs_p": "p.Arg579His",
          "transcript": "ENST00000642580.1",
          "protein_id": "ENSP00000495339.1",
          "transcript_support_level": null,
          "aa_start": 579,
          "aa_end": null,
          "aa_length": 784,
          "cds_start": 1736,
          "cds_end": null,
          "cds_length": 2355,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000642580.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TLR2",
          "gene_hgnc_id": 11848,
          "hgvs_c": "c.1736G>A",
          "hgvs_p": "p.Arg579His",
          "transcript": "ENST00000643501.2",
          "protein_id": "ENSP00000496208.2",
          "transcript_support_level": null,
          "aa_start": 579,
          "aa_end": null,
          "aa_length": 784,
          "cds_start": 1736,
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          "cds_length": 2355,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000643501.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TLR2",
          "gene_hgnc_id": 11848,
          "hgvs_c": "c.1736G>A",
          "hgvs_p": "p.Arg579His",
          "transcript": "ENST00000646219.2",
          "protein_id": "ENSP00000496676.2",
          "transcript_support_level": null,
          "aa_start": 579,
          "aa_end": null,
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          "cds_start": 1736,
          "cds_end": null,
          "cds_length": 2355,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000646219.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TLR2",
          "gene_hgnc_id": 11848,
          "hgvs_c": "c.1736G>A",
          "hgvs_p": "p.Arg579His",
          "transcript": "ENST00000646900.2",
          "protein_id": "ENSP00000493968.2",
          "transcript_support_level": null,
          "aa_start": 579,
          "aa_end": null,
          "aa_length": 784,
          "cds_start": 1736,
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        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": true,
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          ],
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          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TLR2",
          "gene_hgnc_id": 11848,
          "hgvs_c": "c.1736G>A",
          "hgvs_p": "p.Arg579His",
          "transcript": "ENST00000714431.1",
          "protein_id": "ENSP00000519700.1",
          "transcript_support_level": null,
          "aa_start": 579,
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          "cds_start": 1736,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000714431.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
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          "gene_symbol": "TLR2",
          "gene_hgnc_id": 11848,
          "hgvs_c": "c.1736G>A",
          "hgvs_p": "p.Arg579His",
          "transcript": "ENST00000714432.1",
          "protein_id": "ENSP00000519701.1",
          "transcript_support_level": null,
          "aa_start": 579,
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          "cds_start": 1736,
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          "biotype": "protein_coding",
          "feature": "ENST00000714432.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TLR2",
          "gene_hgnc_id": 11848,
          "hgvs_c": "c.1736G>A",
          "hgvs_p": "p.Arg579His",
          "transcript": "ENST00000714433.1",
          "protein_id": "ENSP00000519702.1",
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        {
          "aa_ref": "R",
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          ],
          "exon_rank": 3,
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          "gene_symbol": "TLR2",
          "gene_hgnc_id": 11848,
          "hgvs_c": "c.1736G>A",
          "hgvs_p": "p.Arg579His",
          "transcript": "ENST00000714434.1",
          "protein_id": "ENSP00000519703.1",
          "transcript_support_level": null,
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        {
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          ],
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          "exon_count": 4,
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          "gene_symbol": "TLR2",
          "gene_hgnc_id": 11848,
          "hgvs_c": "c.1736G>A",
          "hgvs_p": "p.Arg579His",
          "transcript": "ENST00000714435.1",
          "protein_id": "ENSP00000519704.1",
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000714435.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TLR2",
          "gene_hgnc_id": 11848,
          "hgvs_c": "c.1736G>A",
          "hgvs_p": "p.Arg579His",
          "transcript": "ENST00000855786.1",
          "protein_id": "ENSP00000525845.1",
          "transcript_support_level": null,
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          "cds_start": 1736,
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        {
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          "gene_symbol": "TLR2",
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          "hgvs_c": "c.598-208G>A",
          "hgvs_p": null,
          "transcript": "ENST00000714436.1",
          "protein_id": "ENSP00000519705.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "cds_length": 600,
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          "biotype": "protein_coding",
          "feature": "ENST00000714436.1"
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      ],
      "gene_symbol": "TLR2",
      "gene_hgnc_id": 11848,
      "dbsnp": "rs5743703",
      "frequency_reference_population": 0.00049774005,
      "hom_count_reference_population": 2,
      "allele_count_reference_population": 803,
      "gnomad_exomes_af": 0.000272359,
      "gnomad_genomes_af": 0.00266475,
      "gnomad_exomes_ac": 398,
      "gnomad_genomes_ac": 405,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 2,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.004875510931015015,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.025,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0596,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.78,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.224,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -16,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BS2",
      "acmg_by_gene": [
        {
          "score": -16,
          "benign_score": 16,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_003264.5",
          "gene_symbol": "TLR2",
          "hgnc_id": 11848,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.1736G>A",
          "hgvs_p": "p.Arg579His"
        }
      ],
      "clinvar_disease": "TLR2-related disorder,not provided",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "B:2",
      "phenotype_combined": "not provided|TLR2-related disorder",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}