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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-1641346-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=1641346&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 1641346,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001013622.3",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAM53A",
          "gene_hgnc_id": 31860,
          "hgvs_c": "c.1144G>A",
          "hgvs_p": "p.Val382Ile",
          "transcript": "NM_001174070.3",
          "protein_id": "NP_001167541.1",
          "transcript_support_level": null,
          "aa_start": 382,
          "aa_end": null,
          "aa_length": 398,
          "cds_start": 1144,
          "cds_end": null,
          "cds_length": 1197,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000308132.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001174070.3"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAM53A",
          "gene_hgnc_id": 31860,
          "hgvs_c": "c.1144G>A",
          "hgvs_p": "p.Val382Ile",
          "transcript": "ENST00000308132.11",
          "protein_id": "ENSP00000310057.6",
          "transcript_support_level": 2,
          "aa_start": 382,
          "aa_end": null,
          "aa_length": 398,
          "cds_start": 1144,
          "cds_end": null,
          "cds_length": 1197,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001174070.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000308132.11"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAM53A",
          "gene_hgnc_id": 31860,
          "hgvs_c": "c.1144G>A",
          "hgvs_p": "p.Val382Ile",
          "transcript": "ENST00000472884.6",
          "protein_id": "ENSP00000426260.1",
          "transcript_support_level": 1,
          "aa_start": 382,
          "aa_end": null,
          "aa_length": 398,
          "cds_start": 1144,
          "cds_end": null,
          "cds_length": 1197,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000472884.6"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAM53A",
          "gene_hgnc_id": 31860,
          "hgvs_c": "c.1144G>A",
          "hgvs_p": "p.Val382Ile",
          "transcript": "NM_001013622.3",
          "protein_id": "NP_001013644.1",
          "transcript_support_level": null,
          "aa_start": 382,
          "aa_end": null,
          "aa_length": 398,
          "cds_start": 1144,
          "cds_end": null,
          "cds_length": 1197,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001013622.3"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAM53A",
          "gene_hgnc_id": 31860,
          "hgvs_c": "c.1144G>A",
          "hgvs_p": "p.Val382Ile",
          "transcript": "ENST00000461064.5",
          "protein_id": "ENSP00000418243.1",
          "transcript_support_level": 2,
          "aa_start": 382,
          "aa_end": null,
          "aa_length": 398,
          "cds_start": 1144,
          "cds_end": null,
          "cds_length": 1197,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000461064.5"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAM53A",
          "gene_hgnc_id": 31860,
          "hgvs_c": "c.1144G>A",
          "hgvs_p": "p.Val382Ile",
          "transcript": "XM_011513402.3",
          "protein_id": "XP_011511704.1",
          "transcript_support_level": null,
          "aa_start": 382,
          "aa_end": null,
          "aa_length": 398,
          "cds_start": 1144,
          "cds_end": null,
          "cds_length": 1197,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011513402.3"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAM53A",
          "gene_hgnc_id": 31860,
          "hgvs_c": "c.1144G>A",
          "hgvs_p": "p.Val382Ile",
          "transcript": "XM_047449654.1",
          "protein_id": "XP_047305610.1",
          "transcript_support_level": null,
          "aa_start": 382,
          "aa_end": null,
          "aa_length": 398,
          "cds_start": 1144,
          "cds_end": null,
          "cds_length": 1197,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047449654.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAM53A",
          "gene_hgnc_id": 31860,
          "hgvs_c": "c.1144G>A",
          "hgvs_p": "p.Val382Ile",
          "transcript": "XM_047449655.1",
          "protein_id": "XP_047305611.1",
          "transcript_support_level": null,
          "aa_start": 382,
          "aa_end": null,
          "aa_length": 398,
          "cds_start": 1144,
          "cds_end": null,
          "cds_length": 1197,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047449655.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAM53A",
          "gene_hgnc_id": 31860,
          "hgvs_c": "c.1144G>A",
          "hgvs_p": "p.Val382Ile",
          "transcript": "XM_047449656.1",
          "protein_id": "XP_047305612.1",
          "transcript_support_level": null,
          "aa_start": 382,
          "aa_end": null,
          "aa_length": 398,
          "cds_start": 1144,
          "cds_end": null,
          "cds_length": 1197,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047449656.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAM53A",
          "gene_hgnc_id": 31860,
          "hgvs_c": "c.1144G>A",
          "hgvs_p": "p.Val382Ile",
          "transcript": "XM_047449657.1",
          "protein_id": "XP_047305613.1",
          "transcript_support_level": null,
          "aa_start": 382,
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          "aa_length": 398,
          "cds_start": 1144,
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          "cdna_start": null,
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          "cdna_length": null,
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          "biotype": "protein_coding",
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        {
          "aa_ref": "V",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
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          "gene_symbol": "FAM53A",
          "gene_hgnc_id": 31860,
          "hgvs_c": "c.1144G>A",
          "hgvs_p": "p.Val382Ile",
          "transcript": "XM_047449658.1",
          "protein_id": "XP_047305614.1",
          "transcript_support_level": null,
          "aa_start": 382,
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          "aa_length": 398,
          "cds_start": 1144,
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          "cds_length": 1197,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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        {
          "aa_ref": "V",
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          "strand": false,
          "consequences": [
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          "intron_rank": null,
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          "hgvs_c": "c.1144G>A",
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          "transcript": "XM_047449659.1",
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          "cds_start": 1144,
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        {
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          "strand": false,
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          ],
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          "gene_symbol": "FAM53A",
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          "cds_start": 1144,
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          "biotype": "protein_coding",
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        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "exon_count": 5,
          "intron_rank": null,
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          "gene_symbol": "FAM53A",
          "gene_hgnc_id": 31860,
          "hgvs_c": "c.1144G>A",
          "hgvs_p": "p.Val382Ile",
          "transcript": "XM_047449661.1",
          "protein_id": "XP_047305617.1",
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        {
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          "intron_rank": null,
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          "gene_symbol": "FAM53A",
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          "hgvs_c": "c.1144G>A",
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          "transcript": "XM_047449662.1",
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        },
        {
          "aa_ref": null,
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          "canonical": false,
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          "consequences": [
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          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
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          "gene_symbol": "FAM53A",
          "gene_hgnc_id": 31860,
          "hgvs_c": "c.*215G>A",
          "hgvs_p": null,
          "transcript": "NM_001297435.1",
          "protein_id": "NP_001284364.1",
          "transcript_support_level": null,
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          "aa_length": 360,
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        },
        {
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          ],
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          "intron_rank": null,
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          "gene_symbol": "FAM53A",
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          "hgvs_c": "c.*215G>A",
          "hgvs_p": null,
          "transcript": "ENST00000489363.5",
          "protein_id": "ENSP00000419044.1",
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          "gene_symbol": "FAM53A",
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          "biotype": "protein_coding",
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        },
        {
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          "canonical": false,
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          "consequences": [
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          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
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          "gene_symbol": "FAM53A",
          "gene_hgnc_id": 31860,
          "hgvs_c": "c.*215G>A",
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          "transcript": "XM_047449665.1",
          "protein_id": "XP_047305621.1",
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      ],
      "gene_symbol": "FAM53A",
      "gene_hgnc_id": 31860,
      "dbsnp": "rs779785935",
      "frequency_reference_population": 0.000009310132,
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      "allele_count_reference_population": 15,
      "gnomad_exomes_af": 0.00000822604,
      "gnomad_genomes_af": 0.0000196894,
      "gnomad_exomes_ac": 12,
      "gnomad_genomes_ac": 3,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.016709446907043457,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.014,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.075,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.76,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -1.489,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
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      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong,BP6_Moderate",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 6,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong",
            "BP6_Moderate"
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          "verdict": "Likely_benign",
          "transcript": "NM_001013622.3",
          "gene_symbol": "FAM53A",
          "hgnc_id": 31860,
          "effects": [
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          "inheritance_mode": "AR",
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          "hgvs_p": "p.Val382Ile"
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Likely benign",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LB:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}