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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-1814554-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=1814554&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 1814554,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_012318.3",
      "consequences": [
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETM1",
          "gene_hgnc_id": 6556,
          "hgvs_c": "c.2090A>G",
          "hgvs_p": "p.Lys697Arg",
          "transcript": "NM_012318.3",
          "protein_id": "NP_036450.1",
          "transcript_support_level": null,
          "aa_start": 697,
          "aa_end": null,
          "aa_length": 739,
          "cds_start": 2090,
          "cds_end": null,
          "cds_length": 2220,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000302787.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_012318.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETM1",
          "gene_hgnc_id": 6556,
          "hgvs_c": "c.2090A>G",
          "hgvs_p": "p.Lys697Arg",
          "transcript": "ENST00000302787.3",
          "protein_id": "ENSP00000305653.2",
          "transcript_support_level": 1,
          "aa_start": 697,
          "aa_end": null,
          "aa_length": 739,
          "cds_start": 2090,
          "cds_end": null,
          "cds_length": 2220,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_012318.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000302787.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETM1",
          "gene_hgnc_id": 6556,
          "hgvs_c": "c.2507A>G",
          "hgvs_p": "p.Lys836Arg",
          "transcript": "ENST00000872452.1",
          "protein_id": "ENSP00000542511.1",
          "transcript_support_level": null,
          "aa_start": 836,
          "aa_end": null,
          "aa_length": 878,
          "cds_start": 2507,
          "cds_end": null,
          "cds_length": 2637,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872452.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETM1",
          "gene_hgnc_id": 6556,
          "hgvs_c": "c.2108A>G",
          "hgvs_p": "p.Lys703Arg",
          "transcript": "ENST00000872451.1",
          "protein_id": "ENSP00000542510.1",
          "transcript_support_level": null,
          "aa_start": 703,
          "aa_end": null,
          "aa_length": 745,
          "cds_start": 2108,
          "cds_end": null,
          "cds_length": 2238,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872451.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETM1",
          "gene_hgnc_id": 6556,
          "hgvs_c": "c.2087A>G",
          "hgvs_p": "p.Lys696Arg",
          "transcript": "ENST00000872446.1",
          "protein_id": "ENSP00000542505.1",
          "transcript_support_level": null,
          "aa_start": 696,
          "aa_end": null,
          "aa_length": 738,
          "cds_start": 2087,
          "cds_end": null,
          "cds_length": 2217,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872446.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETM1",
          "gene_hgnc_id": 6556,
          "hgvs_c": "c.2087A>G",
          "hgvs_p": "p.Lys696Arg",
          "transcript": "ENST00000917095.1",
          "protein_id": "ENSP00000587154.1",
          "transcript_support_level": null,
          "aa_start": 696,
          "aa_end": null,
          "aa_length": 738,
          "cds_start": 2087,
          "cds_end": null,
          "cds_length": 2217,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917095.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETM1",
          "gene_hgnc_id": 6556,
          "hgvs_c": "c.2087A>G",
          "hgvs_p": "p.Lys696Arg",
          "transcript": "ENST00000957530.1",
          "protein_id": "ENSP00000627589.1",
          "transcript_support_level": null,
          "aa_start": 696,
          "aa_end": null,
          "aa_length": 738,
          "cds_start": 2087,
          "cds_end": null,
          "cds_length": 2217,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000957530.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETM1",
          "gene_hgnc_id": 6556,
          "hgvs_c": "c.2084A>G",
          "hgvs_p": "p.Lys695Arg",
          "transcript": "ENST00000957532.1",
          "protein_id": "ENSP00000627591.1",
          "transcript_support_level": null,
          "aa_start": 695,
          "aa_end": null,
          "aa_length": 737,
          "cds_start": 2084,
          "cds_end": null,
          "cds_length": 2214,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000957532.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETM1",
          "gene_hgnc_id": 6556,
          "hgvs_c": "c.2084A>G",
          "hgvs_p": "p.Lys695Arg",
          "transcript": "ENST00000957534.1",
          "protein_id": "ENSP00000627593.1",
          "transcript_support_level": null,
          "aa_start": 695,
          "aa_end": null,
          "aa_length": 737,
          "cds_start": 2084,
          "cds_end": null,
          "cds_length": 2214,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000957534.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETM1",
          "gene_hgnc_id": 6556,
          "hgvs_c": "c.2081A>G",
          "hgvs_p": "p.Lys694Arg",
          "transcript": "ENST00000872448.1",
          "protein_id": "ENSP00000542507.1",
          "transcript_support_level": null,
          "aa_start": 694,
          "aa_end": null,
          "aa_length": 736,
          "cds_start": 2081,
          "cds_end": null,
          "cds_length": 2211,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872448.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETM1",
          "gene_hgnc_id": 6556,
          "hgvs_c": "c.2081A>G",
          "hgvs_p": "p.Lys694Arg",
          "transcript": "ENST00000872450.1",
          "protein_id": "ENSP00000542509.1",
          "transcript_support_level": null,
          "aa_start": 694,
          "aa_end": null,
          "aa_length": 736,
          "cds_start": 2081,
          "cds_end": null,
          "cds_length": 2211,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872450.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETM1",
          "gene_hgnc_id": 6556,
          "hgvs_c": "c.2027A>G",
          "hgvs_p": "p.Lys676Arg",
          "transcript": "ENST00000872454.1",
          "protein_id": "ENSP00000542513.1",
          "transcript_support_level": null,
          "aa_start": 676,
          "aa_end": null,
          "aa_length": 718,
          "cds_start": 2027,
          "cds_end": null,
          "cds_length": 2157,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872454.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETM1",
          "gene_hgnc_id": 6556,
          "hgvs_c": "c.1958A>G",
          "hgvs_p": "p.Lys653Arg",
          "transcript": "ENST00000872453.1",
          "protein_id": "ENSP00000542512.1",
          "transcript_support_level": null,
          "aa_start": 653,
          "aa_end": null,
          "aa_length": 695,
          "cds_start": 1958,
          "cds_end": null,
          "cds_length": 2088,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872453.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETM1",
          "gene_hgnc_id": 6556,
          "hgvs_c": "c.1952A>G",
          "hgvs_p": "p.Lys651Arg",
          "transcript": "ENST00000872449.1",
          "protein_id": "ENSP00000542508.1",
          "transcript_support_level": null,
          "aa_start": 651,
          "aa_end": null,
          "aa_length": 693,
          "cds_start": 1952,
          "cds_end": null,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000872449.1"
        },
        {
          "aa_ref": "K",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETM1",
          "gene_hgnc_id": 6556,
          "hgvs_c": "c.1889A>G",
          "hgvs_p": "p.Lys630Arg",
          "transcript": "ENST00000957533.1",
          "protein_id": "ENSP00000627592.1",
          "transcript_support_level": null,
          "aa_start": 630,
          "aa_end": null,
          "aa_length": 672,
          "cds_start": 1889,
          "cds_end": null,
          "cds_length": 2019,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000957533.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETM1",
          "gene_hgnc_id": 6556,
          "hgvs_c": "c.1886A>G",
          "hgvs_p": "p.Lys629Arg",
          "transcript": "ENST00000872447.1",
          "protein_id": "ENSP00000542506.1",
          "transcript_support_level": null,
          "aa_start": 629,
          "aa_end": null,
          "aa_length": 671,
          "cds_start": 1886,
          "cds_end": null,
          "cds_length": 2016,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872447.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETM1",
          "gene_hgnc_id": 6556,
          "hgvs_c": "c.1883A>G",
          "hgvs_p": "p.Lys628Arg",
          "transcript": "ENST00000957531.1",
          "protein_id": "ENSP00000627590.1",
          "transcript_support_level": null,
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          "aa_end": null,
          "aa_length": 670,
          "cds_start": 1883,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
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          "strand": false,
          "consequences": [
            "missense_variant"
          ],
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          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETM1",
          "gene_hgnc_id": 6556,
          "hgvs_c": "c.1748A>G",
          "hgvs_p": "p.Lys583Arg",
          "transcript": "ENST00000957529.1",
          "protein_id": "ENSP00000627588.1",
          "transcript_support_level": null,
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          "aa_end": null,
          "aa_length": 625,
          "cds_start": 1748,
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          "cds_length": 1878,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "K",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETM1",
          "gene_hgnc_id": 6556,
          "hgvs_c": "c.2087A>G",
          "hgvs_p": "p.Lys696Arg",
          "transcript": "XM_006713884.2",
          "protein_id": "XP_006713947.1",
          "transcript_support_level": null,
          "aa_start": 696,
          "aa_end": null,
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          "cds_start": 2087,
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          "cds_length": 2217,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_006713884.2"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETM1",
          "gene_hgnc_id": 6556,
          "hgvs_c": "c.1547A>G",
          "hgvs_p": "p.Lys516Arg",
          "transcript": "XM_047415673.1",
          "protein_id": "XP_047271629.1",
          "transcript_support_level": null,
          "aa_start": 516,
          "aa_end": null,
          "aa_length": 558,
          "cds_start": 1547,
          "cds_end": null,
          "cds_length": 1677,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047415673.1"
        }
      ],
      "gene_symbol": "LETM1",
      "gene_hgnc_id": 6556,
      "dbsnp": "rs141679322",
      "frequency_reference_population": 0.0007566903,
      "hom_count_reference_population": 3,
      "allele_count_reference_population": 1221,
      "gnomad_exomes_af": 0.000401034,
      "gnomad_genomes_af": 0.00416716,
      "gnomad_exomes_ac": 586,
      "gnomad_genomes_ac": 635,
      "gnomad_exomes_homalt": 2,
      "gnomad_genomes_homalt": 1,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.0075158774852752686,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.108,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1001,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.26,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 7.477,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -20,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BS1,BS2",
      "acmg_by_gene": [
        {
          "score": -20,
          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BS1",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_012318.3",
          "gene_symbol": "LETM1",
          "hgnc_id": 6556,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2090A>G",
          "hgvs_p": "p.Lys697Arg"
        }
      ],
      "clinvar_disease": "not provided",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "B:2",
      "phenotype_combined": "not provided",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}