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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-24819861-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=24819861&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 24819861,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant,splice_region_variant",
      "transcript": "NM_001395273.1",
      "consequences": [
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC149",
          "gene_hgnc_id": 25405,
          "hgvs_c": "c.1175A>G",
          "hgvs_p": "p.Asp392Gly",
          "transcript": "NM_001395273.1",
          "protein_id": "NP_001382202.1",
          "transcript_support_level": null,
          "aa_start": 392,
          "aa_end": null,
          "aa_length": 535,
          "cds_start": 1175,
          "cds_end": null,
          "cds_length": 1608,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000635206.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001395273.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC149",
          "gene_hgnc_id": 25405,
          "hgvs_c": "c.1175A>G",
          "hgvs_p": "p.Asp392Gly",
          "transcript": "ENST00000635206.3",
          "protein_id": "ENSP00000488929.2",
          "transcript_support_level": 5,
          "aa_start": 392,
          "aa_end": null,
          "aa_length": 535,
          "cds_start": 1175,
          "cds_end": null,
          "cds_length": 1608,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001395273.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000635206.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "CCDC149",
          "gene_hgnc_id": 25405,
          "hgvs_c": "c.358-11042A>G",
          "hgvs_p": null,
          "transcript": "ENST00000502801.1",
          "protein_id": "ENSP00000427529.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 136,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 411,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000502801.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC149",
          "gene_hgnc_id": 25405,
          "hgvs_c": "c.1166A>G",
          "hgvs_p": "p.Asp389Gly",
          "transcript": "ENST00000904727.1",
          "protein_id": "ENSP00000574786.1",
          "transcript_support_level": null,
          "aa_start": 389,
          "aa_end": null,
          "aa_length": 532,
          "cds_start": 1166,
          "cds_end": null,
          "cds_length": 1599,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000904727.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC149",
          "gene_hgnc_id": 25405,
          "hgvs_c": "c.1157A>G",
          "hgvs_p": "p.Asp386Gly",
          "transcript": "NM_173463.6",
          "protein_id": "NP_775734.2",
          "transcript_support_level": null,
          "aa_start": 386,
          "aa_end": null,
          "aa_length": 529,
          "cds_start": 1157,
          "cds_end": null,
          "cds_length": 1590,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_173463.6"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC149",
          "gene_hgnc_id": 25405,
          "hgvs_c": "c.1157A>G",
          "hgvs_p": "p.Asp386Gly",
          "transcript": "ENST00000389609.8",
          "protein_id": "ENSP00000374260.4",
          "transcript_support_level": 2,
          "aa_start": 386,
          "aa_end": null,
          "aa_length": 529,
          "cds_start": 1157,
          "cds_end": null,
          "cds_length": 1590,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000389609.8"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC149",
          "gene_hgnc_id": 25405,
          "hgvs_c": "c.1142A>G",
          "hgvs_p": "p.Asp381Gly",
          "transcript": "NM_001130726.5",
          "protein_id": "NP_001124198.2",
          "transcript_support_level": null,
          "aa_start": 381,
          "aa_end": null,
          "aa_length": 524,
          "cds_start": 1142,
          "cds_end": null,
          "cds_length": 1575,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001130726.5"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC149",
          "gene_hgnc_id": 25405,
          "hgvs_c": "c.1142A>G",
          "hgvs_p": "p.Asp381Gly",
          "transcript": "ENST00000504487.5",
          "protein_id": "ENSP00000425715.2",
          "transcript_support_level": 2,
          "aa_start": 381,
          "aa_end": null,
          "aa_length": 524,
          "cds_start": 1142,
          "cds_end": null,
          "cds_length": 1575,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000504487.5"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC149",
          "gene_hgnc_id": 25405,
          "hgvs_c": "c.1133A>G",
          "hgvs_p": "p.Asp378Gly",
          "transcript": "ENST00000904725.1",
          "protein_id": "ENSP00000574784.1",
          "transcript_support_level": null,
          "aa_start": 378,
          "aa_end": null,
          "aa_length": 521,
          "cds_start": 1133,
          "cds_end": null,
          "cds_length": 1566,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000904725.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC149",
          "gene_hgnc_id": 25405,
          "hgvs_c": "c.1025A>G",
          "hgvs_p": "p.Asp342Gly",
          "transcript": "ENST00000914099.1",
          "protein_id": "ENSP00000584158.1",
          "transcript_support_level": null,
          "aa_start": 342,
          "aa_end": null,
          "aa_length": 485,
          "cds_start": 1025,
          "cds_end": null,
          "cds_length": 1458,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000914099.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC149",
          "gene_hgnc_id": 25405,
          "hgvs_c": "c.992A>G",
          "hgvs_p": "p.Asp331Gly",
          "transcript": "NM_001330644.2",
          "protein_id": "NP_001317573.1",
          "transcript_support_level": null,
          "aa_start": 331,
          "aa_end": null,
          "aa_length": 474,
          "cds_start": 992,
          "cds_end": null,
          "cds_length": 1425,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001330644.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC149",
          "gene_hgnc_id": 25405,
          "hgvs_c": "c.974A>G",
          "hgvs_p": "p.Asp325Gly",
          "transcript": "ENST00000904724.1",
          "protein_id": "ENSP00000574783.1",
          "transcript_support_level": null,
          "aa_start": 325,
          "aa_end": null,
          "aa_length": 468,
          "cds_start": 974,
          "cds_end": null,
          "cds_length": 1407,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000904724.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC149",
          "gene_hgnc_id": 25405,
          "hgvs_c": "c.941A>G",
          "hgvs_p": "p.Asp314Gly",
          "transcript": "ENST00000904726.1",
          "protein_id": "ENSP00000574785.1",
          "transcript_support_level": null,
          "aa_start": 314,
          "aa_end": null,
          "aa_length": 457,
          "cds_start": 941,
          "cds_end": null,
          "cds_length": 1374,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000904726.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC149",
          "gene_hgnc_id": 25405,
          "hgvs_c": "c.896A>G",
          "hgvs_p": "p.Asp299Gly",
          "transcript": "ENST00000951414.1",
          "protein_id": "ENSP00000621473.1",
          "transcript_support_level": null,
          "aa_start": 299,
          "aa_end": null,
          "aa_length": 442,
          "cds_start": 896,
          "cds_end": null,
          "cds_length": 1329,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000951414.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC149",
          "gene_hgnc_id": 25405,
          "hgvs_c": "c.1190A>G",
          "hgvs_p": "p.Asp397Gly",
          "transcript": "XM_011513906.4",
          "protein_id": "XP_011512208.1",
          "transcript_support_level": null,
          "aa_start": 397,
          "aa_end": null,
          "aa_length": 540,
          "cds_start": 1190,
          "cds_end": null,
          "cds_length": 1623,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011513906.4"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC149",
          "gene_hgnc_id": 25405,
          "hgvs_c": "c.1157A>G",
          "hgvs_p": "p.Asp386Gly",
          "transcript": "XM_017008827.3",
          "protein_id": "XP_016864316.1",
          "transcript_support_level": null,
          "aa_start": 386,
          "aa_end": null,
          "aa_length": 529,
          "cds_start": 1157,
          "cds_end": null,
          "cds_length": 1590,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017008827.3"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC149",
          "gene_hgnc_id": 25405,
          "hgvs_c": "c.1025A>G",
          "hgvs_p": "p.Asp342Gly",
          "transcript": "XM_011513907.4",
          "protein_id": "XP_011512209.1",
          "transcript_support_level": null,
          "aa_start": 342,
          "aa_end": null,
          "aa_length": 485,
          "cds_start": 1025,
          "cds_end": null,
          "cds_length": 1458,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011513907.4"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC149",
          "gene_hgnc_id": 25405,
          "hgvs_c": "c.1025A>G",
          "hgvs_p": "p.Asp342Gly",
          "transcript": "XM_011513908.3",
          "protein_id": "XP_011512210.1",
          "transcript_support_level": null,
          "aa_start": 342,
          "aa_end": null,
          "aa_length": 485,
          "cds_start": 1025,
          "cds_end": null,
          "cds_length": 1458,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011513908.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC149",
          "gene_hgnc_id": 25405,
          "hgvs_c": "n.337A>G",
          "hgvs_p": null,
          "transcript": "ENST00000507096.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000507096.1"
        }
      ],
      "gene_symbol": "CCDC149",
      "gene_hgnc_id": 25405,
      "dbsnp": "rs191079849",
      "frequency_reference_population": 0.000025153955,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 39,
      "gnomad_exomes_af": 0.0000235997,
      "gnomad_genomes_af": 0.0000394405,
      "gnomad_exomes_ac": 33,
      "gnomad_genomes_ac": 6,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.007038086652755737,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.00800000037997961,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "dbscSNV1_RF",
      "revel_score": 0.048,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0654,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.53,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.363,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.09,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": 0.000331873234187629,
      "dbscsnv_ada_prediction": "Benign",
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001395273.1",
          "gene_symbol": "CCDC149",
          "hgnc_id": 25405,
          "effects": [
            "missense_variant",
            "splice_region_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1175A>G",
          "hgvs_p": "p.Asp392Gly"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}