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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-26420695-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=26420695&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 26420695,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_005349.4",
      "consequences": [
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBPJ",
          "gene_hgnc_id": 5724,
          "hgvs_c": "c.466A>G",
          "hgvs_p": "p.Lys156Glu",
          "transcript": "NM_015874.6",
          "protein_id": "NP_056958.3",
          "transcript_support_level": null,
          "aa_start": 156,
          "aa_end": null,
          "aa_length": 487,
          "cds_start": 466,
          "cds_end": null,
          "cds_length": 1464,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000355476.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_015874.6"
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBPJ",
          "gene_hgnc_id": 5724,
          "hgvs_c": "c.466A>G",
          "hgvs_p": "p.Lys156Glu",
          "transcript": "ENST00000355476.8",
          "protein_id": "ENSP00000347659.4",
          "transcript_support_level": 1,
          "aa_start": 156,
          "aa_end": null,
          "aa_length": 487,
          "cds_start": 466,
          "cds_end": null,
          "cds_length": 1464,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_015874.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000355476.8"
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBPJ",
          "gene_hgnc_id": 5724,
          "hgvs_c": "c.505A>G",
          "hgvs_p": "p.Lys169Glu",
          "transcript": "ENST00000361572.10",
          "protein_id": "ENSP00000354528.6",
          "transcript_support_level": 1,
          "aa_start": 169,
          "aa_end": null,
          "aa_length": 500,
          "cds_start": 505,
          "cds_end": null,
          "cds_length": 1503,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000361572.10"
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBPJ",
          "gene_hgnc_id": 5724,
          "hgvs_c": "c.463A>G",
          "hgvs_p": "p.Lys155Glu",
          "transcript": "ENST00000342320.8",
          "protein_id": "ENSP00000340124.4",
          "transcript_support_level": 1,
          "aa_start": 155,
          "aa_end": null,
          "aa_length": 486,
          "cds_start": 463,
          "cds_end": null,
          "cds_length": 1461,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000342320.8"
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBPJ",
          "gene_hgnc_id": 5724,
          "hgvs_c": "c.463A>G",
          "hgvs_p": "p.Lys155Glu",
          "transcript": "ENST00000348160.9",
          "protein_id": "ENSP00000339699.5",
          "transcript_support_level": 1,
          "aa_start": 155,
          "aa_end": null,
          "aa_length": 486,
          "cds_start": 463,
          "cds_end": null,
          "cds_length": 1461,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000348160.9"
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBPJ",
          "gene_hgnc_id": 5724,
          "hgvs_c": "c.400A>G",
          "hgvs_p": "p.Lys134Glu",
          "transcript": "ENST00000345843.8",
          "protein_id": "ENSP00000305815.6",
          "transcript_support_level": 1,
          "aa_start": 134,
          "aa_end": null,
          "aa_length": 465,
          "cds_start": 400,
          "cds_end": null,
          "cds_length": 1398,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000345843.8"
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBPJ",
          "gene_hgnc_id": 5724,
          "hgvs_c": "c.400A>G",
          "hgvs_p": "p.Lys134Glu",
          "transcript": "ENST00000507561.5",
          "protein_id": "ENSP00000423907.1",
          "transcript_support_level": 1,
          "aa_start": 134,
          "aa_end": null,
          "aa_length": 465,
          "cds_start": 400,
          "cds_end": null,
          "cds_length": 1398,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000507561.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBPJ",
          "gene_hgnc_id": 5724,
          "hgvs_c": "c.505A>G",
          "hgvs_p": "p.Lys169Glu",
          "transcript": "NM_001374400.1",
          "protein_id": "NP_001361329.1",
          "transcript_support_level": null,
          "aa_start": 169,
          "aa_end": null,
          "aa_length": 500,
          "cds_start": 505,
          "cds_end": null,
          "cds_length": 1503,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001374400.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBPJ",
          "gene_hgnc_id": 5724,
          "hgvs_c": "c.505A>G",
          "hgvs_p": "p.Lys169Glu",
          "transcript": "NM_005349.4",
          "protein_id": "NP_005340.2",
          "transcript_support_level": null,
          "aa_start": 169,
          "aa_end": null,
          "aa_length": 500,
          "cds_start": 505,
          "cds_end": null,
          "cds_length": 1503,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_005349.4"
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBPJ",
          "gene_hgnc_id": 5724,
          "hgvs_c": "c.505A>G",
          "hgvs_p": "p.Lys169Glu",
          "transcript": "ENST00000342295.6",
          "protein_id": "ENSP00000345206.1",
          "transcript_support_level": 5,
          "aa_start": 169,
          "aa_end": null,
          "aa_length": 500,
          "cds_start": 505,
          "cds_end": null,
          "cds_length": 1503,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000342295.6"
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBPJ",
          "gene_hgnc_id": 5724,
          "hgvs_c": "c.505A>G",
          "hgvs_p": "p.Lys169Glu",
          "transcript": "ENST00000512671.6",
          "protein_id": "ENSP00000423644.2",
          "transcript_support_level": 2,
          "aa_start": 169,
          "aa_end": null,
          "aa_length": 500,
          "cds_start": 505,
          "cds_end": null,
          "cds_length": 1503,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000512671.6"
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBPJ",
          "gene_hgnc_id": 5724,
          "hgvs_c": "c.463A>G",
          "hgvs_p": "p.Lys155Glu",
          "transcript": "NM_001374401.1",
          "protein_id": "NP_001361330.1",
          "transcript_support_level": null,
          "aa_start": 155,
          "aa_end": null,
          "aa_length": 486,
          "cds_start": 463,
          "cds_end": null,
          "cds_length": 1461,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001374401.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBPJ",
          "gene_hgnc_id": 5724,
          "hgvs_c": "c.463A>G",
          "hgvs_p": "p.Lys155Glu",
          "transcript": "NM_001374402.1",
          "protein_id": "NP_001361331.1",
          "transcript_support_level": null,
          "aa_start": 155,
          "aa_end": null,
          "aa_length": 486,
          "cds_start": 463,
          "cds_end": null,
          "cds_length": 1461,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001374402.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBPJ",
          "gene_hgnc_id": 5724,
          "hgvs_c": "c.463A>G",
          "hgvs_p": "p.Lys155Glu",
          "transcript": "NM_001374403.1",
          "protein_id": "NP_001361332.1",
          "transcript_support_level": null,
          "aa_start": 155,
          "aa_end": null,
          "aa_length": 486,
          "cds_start": 463,
          "cds_end": null,
          "cds_length": 1461,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001374403.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBPJ",
          "gene_hgnc_id": 5724,
          "hgvs_c": "c.463A>G",
          "hgvs_p": "p.Lys155Glu",
          "transcript": "NM_001379406.1",
          "protein_id": "NP_001366335.1",
          "transcript_support_level": null,
          "aa_start": 155,
          "aa_end": null,
          "aa_length": 486,
          "cds_start": 463,
          "cds_end": null,
          "cds_length": 1461,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001379406.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBPJ",
          "gene_hgnc_id": 5724,
          "hgvs_c": "c.463A>G",
          "hgvs_p": "p.Lys155Glu",
          "transcript": "NM_001379407.1",
          "protein_id": "NP_001366336.1",
          "transcript_support_level": null,
          "aa_start": 155,
          "aa_end": null,
          "aa_length": 486,
          "cds_start": 463,
          "cds_end": null,
          "cds_length": 1461,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001379407.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBPJ",
          "gene_hgnc_id": 5724,
          "hgvs_c": "c.463A>G",
          "hgvs_p": "p.Lys155Glu",
          "transcript": "NM_203284.3",
          "protein_id": "NP_976029.1",
          "transcript_support_level": null,
          "aa_start": 155,
          "aa_end": null,
          "aa_length": 486,
          "cds_start": 463,
          "cds_end": null,
          "cds_length": 1461,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_203284.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBPJ",
          "gene_hgnc_id": 5724,
          "hgvs_c": "c.463A>G",
          "hgvs_p": "p.Lys155Glu",
          "transcript": "ENST00000505958.6",
          "protein_id": "ENSP00000426872.2",
          "transcript_support_level": 5,
          "aa_start": 155,
          "aa_end": null,
          "aa_length": 486,
          "cds_start": 463,
          "cds_end": null,
          "cds_length": 1461,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000505958.6"
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBPJ",
          "gene_hgnc_id": 5724,
          "hgvs_c": "c.463A>G",
          "hgvs_p": "p.Lys155Glu",
          "transcript": "ENST00000680928.1",
          "protein_id": "ENSP00000505493.1",
          "transcript_support_level": null,
          "aa_start": 155,
          "aa_end": null,
          "aa_length": 486,
          "cds_start": 463,
          "cds_end": null,
          "cds_length": 1461,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000680928.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBPJ",
          "gene_hgnc_id": 5724,
          "hgvs_c": "c.463A>G",
          "hgvs_p": "p.Lys155Glu",
          "transcript": "ENST00000681093.1",
          "protein_id": "ENSP00000504964.1",
          "transcript_support_level": null,
          "aa_start": 155,
          "aa_end": null,
          "aa_length": 486,
          "cds_start": 463,
          "cds_end": null,
          "cds_length": 1461,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000681093.1"
        },
        {
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      ],
      "gene_symbol": "RBPJ",
      "gene_hgnc_id": 5724,
      "dbsnp": "rs387907271",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8814659118652344,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.896,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9998,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.4,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.132,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 5,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3_Moderate,PP5",
      "acmg_by_gene": [
        {
          "score": 5,
          "benign_score": 0,
          "pathogenic_score": 5,
          "criteria": [
            "PM2",
            "PP3_Moderate",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_005349.4",
          "gene_symbol": "RBPJ",
          "hgnc_id": 5724,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.505A>G",
          "hgvs_p": "p.Lys169Glu"
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      ],
      "clinvar_disease": "Adams-Oliver syndrome 3",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "Adams-Oliver syndrome 3",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}