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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-39288823-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=39288823&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 39288823,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_001204747.2",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.3382C>T",
          "hgvs_p": "p.Pro1128Ser",
          "transcript": "NM_002913.5",
          "protein_id": "NP_002904.3",
          "transcript_support_level": null,
          "aa_start": 1128,
          "aa_end": null,
          "aa_length": 1147,
          "cds_start": 3382,
          "cds_end": null,
          "cds_length": 3444,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000349703.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_002913.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.3382C>T",
          "hgvs_p": "p.Pro1128Ser",
          "transcript": "ENST00000349703.7",
          "protein_id": "ENSP00000261424.4",
          "transcript_support_level": 1,
          "aa_start": 1128,
          "aa_end": null,
          "aa_length": 1147,
          "cds_start": 3382,
          "cds_end": null,
          "cds_length": 3444,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_002913.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000349703.7"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.3385C>T",
          "hgvs_p": "p.Pro1129Ser",
          "transcript": "ENST00000381897.5",
          "protein_id": "ENSP00000371321.1",
          "transcript_support_level": 1,
          "aa_start": 1129,
          "aa_end": null,
          "aa_length": 1148,
          "cds_start": 3385,
          "cds_end": null,
          "cds_length": 3447,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000381897.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.3430C>T",
          "hgvs_p": "p.Pro1144Ser",
          "transcript": "ENST00000906184.1",
          "protein_id": "ENSP00000576243.1",
          "transcript_support_level": null,
          "aa_start": 1144,
          "aa_end": null,
          "aa_length": 1163,
          "cds_start": 3430,
          "cds_end": null,
          "cds_length": 3492,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000906184.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.3418C>T",
          "hgvs_p": "p.Pro1140Ser",
          "transcript": "ENST00000967837.1",
          "protein_id": "ENSP00000637896.1",
          "transcript_support_level": null,
          "aa_start": 1140,
          "aa_end": null,
          "aa_length": 1159,
          "cds_start": 3418,
          "cds_end": null,
          "cds_length": 3480,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000967837.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.3385C>T",
          "hgvs_p": "p.Pro1129Ser",
          "transcript": "NM_001204747.2",
          "protein_id": "NP_001191676.1",
          "transcript_support_level": null,
          "aa_start": 1129,
          "aa_end": null,
          "aa_length": 1148,
          "cds_start": 3385,
          "cds_end": null,
          "cds_length": 3447,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001204747.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.3331C>T",
          "hgvs_p": "p.Pro1111Ser",
          "transcript": "ENST00000923045.1",
          "protein_id": "ENSP00000593103.1",
          "transcript_support_level": null,
          "aa_start": 1111,
          "aa_end": null,
          "aa_length": 1130,
          "cds_start": 3331,
          "cds_end": null,
          "cds_length": 3393,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000923045.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.3307C>T",
          "hgvs_p": "p.Pro1103Ser",
          "transcript": "NM_001363496.2",
          "protein_id": "NP_001350425.1",
          "transcript_support_level": null,
          "aa_start": 1103,
          "aa_end": null,
          "aa_length": 1122,
          "cds_start": 3307,
          "cds_end": null,
          "cds_length": 3369,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001363496.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.3307C>T",
          "hgvs_p": "p.Pro1103Ser",
          "transcript": "ENST00000906185.1",
          "protein_id": "ENSP00000576244.1",
          "transcript_support_level": null,
          "aa_start": 1103,
          "aa_end": null,
          "aa_length": 1122,
          "cds_start": 3307,
          "cds_end": null,
          "cds_length": 3369,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000906185.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.3307C>T",
          "hgvs_p": "p.Pro1103Ser",
          "transcript": "ENST00000923043.1",
          "protein_id": "ENSP00000593102.1",
          "transcript_support_level": null,
          "aa_start": 1103,
          "aa_end": null,
          "aa_length": 1122,
          "cds_start": 3307,
          "cds_end": null,
          "cds_length": 3369,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000923043.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.3304C>T",
          "hgvs_p": "p.Pro1102Ser",
          "transcript": "NM_001363495.2",
          "protein_id": "NP_001350424.1",
          "transcript_support_level": null,
          "aa_start": 1102,
          "aa_end": null,
          "aa_length": 1121,
          "cds_start": 3304,
          "cds_end": null,
          "cds_length": 3366,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001363495.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.3304C>T",
          "hgvs_p": "p.Pro1102Ser",
          "transcript": "ENST00000906183.1",
          "protein_id": "ENSP00000576242.1",
          "transcript_support_level": null,
          "aa_start": 1102,
          "aa_end": null,
          "aa_length": 1121,
          "cds_start": 3304,
          "cds_end": null,
          "cds_length": 3366,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000906183.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.3304C>T",
          "hgvs_p": "p.Pro1102Ser",
          "transcript": "ENST00000906186.1",
          "protein_id": "ENSP00000576245.1",
          "transcript_support_level": null,
          "aa_start": 1102,
          "aa_end": null,
          "aa_length": 1121,
          "cds_start": 3304,
          "cds_end": null,
          "cds_length": 3366,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000906186.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.3229C>T",
          "hgvs_p": "p.Pro1077Ser",
          "transcript": "ENST00000923042.1",
          "protein_id": "ENSP00000593101.1",
          "transcript_support_level": null,
          "aa_start": 1077,
          "aa_end": null,
          "aa_length": 1096,
          "cds_start": 3229,
          "cds_end": null,
          "cds_length": 3291,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000923042.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.2932C>T",
          "hgvs_p": "p.Pro978Ser",
          "transcript": "ENST00000967836.1",
          "protein_id": "ENSP00000637895.1",
          "transcript_support_level": null,
          "aa_start": 978,
          "aa_end": null,
          "aa_length": 997,
          "cds_start": 2932,
          "cds_end": null,
          "cds_length": 2994,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000967836.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.2590C>T",
          "hgvs_p": "p.Pro864Ser",
          "transcript": "ENST00000923046.1",
          "protein_id": "ENSP00000593105.1",
          "transcript_support_level": null,
          "aa_start": 864,
          "aa_end": null,
          "aa_length": 883,
          "cds_start": 2590,
          "cds_end": null,
          "cds_length": 2652,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000923046.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.2875C>T",
          "hgvs_p": "p.Pro959Ser",
          "transcript": "XM_011513731.2",
          "protein_id": "XP_011512033.1",
          "transcript_support_level": null,
          "aa_start": 959,
          "aa_end": null,
          "aa_length": 978,
          "cds_start": 2875,
          "cds_end": null,
          "cds_length": 2937,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011513731.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.2797C>T",
          "hgvs_p": "p.Pro933Ser",
          "transcript": "XM_047416054.1",
          "protein_id": "XP_047272010.1",
          "transcript_support_level": null,
          "aa_start": 933,
          "aa_end": null,
          "aa_length": 952,
          "cds_start": 2797,
          "cds_end": null,
          "cds_length": 2859,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047416054.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "n.523C>T",
          "hgvs_p": null,
          "transcript": "ENST00000502991.5",
          "protein_id": null,
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000502991.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "n.487C>T",
          "hgvs_p": null,
          "transcript": "ENST00000504974.1",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000504974.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "n.597C>T",
          "hgvs_p": null,
          "transcript": "ENST00000510783.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000510783.5"
        }
      ],
      "gene_symbol": "RFC1",
      "gene_hgnc_id": 9969,
      "dbsnp": "rs755561864",
      "frequency_reference_population": 0.000015526697,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 25,
      "gnomad_exomes_af": 0.0000157646,
      "gnomad_genomes_af": 0.0000132308,
      "gnomad_exomes_ac": 23,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.014418721199035645,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.071,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0662,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.68,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.342,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001204747.2",
          "gene_symbol": "RFC1",
          "hgnc_id": 9969,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.3385C>T",
          "hgvs_p": "p.Pro1129Ser"
        }
      ],
      "clinvar_disease": "Inborn genetic diseases",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "Inborn genetic diseases",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}