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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-48505564-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=48505564&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 48505564,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_015030.2",
      "consequences": [
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 60,
          "exon_rank_end": null,
          "exon_count": 64,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRYL",
          "gene_hgnc_id": 29127,
          "hgvs_c": "c.8446A>G",
          "hgvs_p": "p.Ile2816Val",
          "transcript": "NM_015030.2",
          "protein_id": "NP_055845.1",
          "transcript_support_level": null,
          "aa_start": 2816,
          "aa_end": null,
          "aa_length": 3013,
          "cds_start": 8446,
          "cds_end": null,
          "cds_length": 9042,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000358350.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_015030.2"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 60,
          "exon_rank_end": null,
          "exon_count": 64,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRYL",
          "gene_hgnc_id": 29127,
          "hgvs_c": "c.8446A>G",
          "hgvs_p": "p.Ile2816Val",
          "transcript": "ENST00000358350.9",
          "protein_id": "ENSP00000351113.4",
          "transcript_support_level": 5,
          "aa_start": 2816,
          "aa_end": null,
          "aa_length": 3013,
          "cds_start": 8446,
          "cds_end": null,
          "cds_length": 9042,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_015030.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000358350.9"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 57,
          "exon_rank_end": null,
          "exon_count": 60,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRYL",
          "gene_hgnc_id": 29127,
          "hgvs_c": "c.8446A>G",
          "hgvs_p": "p.Ile2816Val",
          "transcript": "ENST00000507873.8",
          "protein_id": "ENSP00000422408.4",
          "transcript_support_level": 1,
          "aa_start": 2816,
          "aa_end": null,
          "aa_length": 3007,
          "cds_start": 8446,
          "cds_end": null,
          "cds_length": 9024,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000507873.8"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRYL",
          "gene_hgnc_id": 29127,
          "hgvs_c": "n.919A>G",
          "hgvs_p": null,
          "transcript": "ENST00000512810.1",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000512810.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 61,
          "exon_rank_end": null,
          "exon_count": 64,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRYL",
          "gene_hgnc_id": 29127,
          "hgvs_c": "c.8491A>G",
          "hgvs_p": "p.Ile2831Val",
          "transcript": "ENST00000926452.1",
          "protein_id": "ENSP00000596511.1",
          "transcript_support_level": null,
          "aa_start": 2831,
          "aa_end": null,
          "aa_length": 3022,
          "cds_start": 8491,
          "cds_end": null,
          "cds_length": 9069,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000926452.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRYL",
          "gene_hgnc_id": 29127,
          "hgvs_c": "c.4093A>G",
          "hgvs_p": "p.Ile1365Val",
          "transcript": "ENST00000641795.1",
          "protein_id": "ENSP00000493144.1",
          "transcript_support_level": null,
          "aa_start": 1365,
          "aa_end": null,
          "aa_length": 1562,
          "cds_start": 4093,
          "cds_end": null,
          "cds_length": 4689,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000641795.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 59,
          "exon_rank_end": null,
          "exon_count": 63,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRYL",
          "gene_hgnc_id": 29127,
          "hgvs_c": "c.8767A>G",
          "hgvs_p": "p.Ile2923Val",
          "transcript": "XM_024453988.2",
          "protein_id": "XP_024309756.1",
          "transcript_support_level": null,
          "aa_start": 2923,
          "aa_end": null,
          "aa_length": 3120,
          "cds_start": 8767,
          "cds_end": null,
          "cds_length": 9363,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_024453988.2"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 59,
          "exon_rank_end": null,
          "exon_count": 63,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRYL",
          "gene_hgnc_id": 29127,
          "hgvs_c": "c.8764A>G",
          "hgvs_p": "p.Ile2922Val",
          "transcript": "XM_011513680.4",
          "protein_id": "XP_011511982.2",
          "transcript_support_level": null,
          "aa_start": 2922,
          "aa_end": null,
          "aa_length": 3119,
          "cds_start": 8764,
          "cds_end": null,
          "cds_length": 9360,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011513680.4"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 59,
          "exon_rank_end": null,
          "exon_count": 62,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRYL",
          "gene_hgnc_id": 29127,
          "hgvs_c": "c.8767A>G",
          "hgvs_p": "p.Ile2923Val",
          "transcript": "XM_011513681.4",
          "protein_id": "XP_011511983.2",
          "transcript_support_level": null,
          "aa_start": 2923,
          "aa_end": null,
          "aa_length": 3114,
          "cds_start": 8767,
          "cds_end": null,
          "cds_length": 9345,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011513681.4"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 59,
          "exon_rank_end": null,
          "exon_count": 62,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRYL",
          "gene_hgnc_id": 29127,
          "hgvs_c": "c.8764A>G",
          "hgvs_p": "p.Ile2922Val",
          "transcript": "XM_047450095.1",
          "protein_id": "XP_047306051.1",
          "transcript_support_level": null,
          "aa_start": 2922,
          "aa_end": null,
          "aa_length": 3113,
          "cds_start": 8764,
          "cds_end": null,
          "cds_length": 9342,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047450095.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 58,
          "exon_rank_end": null,
          "exon_count": 62,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRYL",
          "gene_hgnc_id": 29127,
          "hgvs_c": "c.8722A>G",
          "hgvs_p": "p.Ile2908Val",
          "transcript": "XM_024453989.2",
          "protein_id": "XP_024309757.1",
          "transcript_support_level": null,
          "aa_start": 2908,
          "aa_end": null,
          "aa_length": 3105,
          "cds_start": 8722,
          "cds_end": null,
          "cds_length": 9318,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_024453989.2"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 58,
          "exon_rank_end": null,
          "exon_count": 62,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRYL",
          "gene_hgnc_id": 29127,
          "hgvs_c": "c.8719A>G",
          "hgvs_p": "p.Ile2907Val",
          "transcript": "XM_047450097.1",
          "protein_id": "XP_047306053.1",
          "transcript_support_level": null,
          "aa_start": 2907,
          "aa_end": null,
          "aa_length": 3104,
          "cds_start": 8719,
          "cds_end": null,
          "cds_length": 9315,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 58,
          "exon_rank_end": null,
          "exon_count": 61,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRYL",
          "gene_hgnc_id": 29127,
          "hgvs_c": "c.8722A>G",
          "hgvs_p": "p.Ile2908Val",
          "transcript": "XM_011513682.4",
          "protein_id": "XP_011511984.2",
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          "aa_start": 2908,
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          "cds_start": 8722,
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          "cds_length": 9300,
          "cdna_start": null,
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          "cdna_length": null,
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          "biotype": "protein_coding",
          "feature": "XM_011513682.4"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 58,
          "exon_rank_end": null,
          "exon_count": 61,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRYL",
          "gene_hgnc_id": 29127,
          "hgvs_c": "c.8719A>G",
          "hgvs_p": "p.Ile2907Val",
          "transcript": "XM_047450098.1",
          "protein_id": "XP_047306054.1",
          "transcript_support_level": null,
          "aa_start": 2907,
          "aa_end": null,
          "aa_length": 3098,
          "cds_start": 8719,
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          "mane_select": null,
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        },
        {
          "aa_ref": "I",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "exon_rank_end": null,
          "exon_count": 65,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRYL",
          "gene_hgnc_id": 29127,
          "hgvs_c": "c.8491A>G",
          "hgvs_p": "p.Ile2831Val",
          "transcript": "XM_005248090.6",
          "protein_id": "XP_005248147.1",
          "transcript_support_level": null,
          "aa_start": 2831,
          "aa_end": null,
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          "cds_start": 8491,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_005248090.6"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 60,
          "exon_rank_end": null,
          "exon_count": 64,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRYL",
          "gene_hgnc_id": 29127,
          "hgvs_c": "c.8491A>G",
          "hgvs_p": "p.Ile2831Val",
          "transcript": "XM_011513683.4",
          "protein_id": "XP_011511985.1",
          "transcript_support_level": null,
          "aa_start": 2831,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "I",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 60,
          "exon_rank_end": null,
          "exon_count": 64,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRYL",
          "gene_hgnc_id": 29127,
          "hgvs_c": "c.8491A>G",
          "hgvs_p": "p.Ile2831Val",
          "transcript": "XM_024453991.2",
          "protein_id": "XP_024309759.1",
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        {
          "aa_ref": "I",
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          ],
          "exon_rank": 59,
          "exon_rank_end": null,
          "exon_count": 63,
          "intron_rank": null,
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          "gene_symbol": "FRYL",
          "gene_hgnc_id": 29127,
          "hgvs_c": "c.8491A>G",
          "hgvs_p": "p.Ile2831Val",
          "transcript": "XM_024453992.2",
          "protein_id": "XP_024309760.1",
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          "biotype": "protein_coding",
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        {
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 60,
          "exon_rank_end": null,
          "exon_count": 64,
          "intron_rank": null,
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          "gene_symbol": "FRYL",
          "gene_hgnc_id": 29127,
          "hgvs_c": "c.8491A>G",
          "hgvs_p": "p.Ile2831Val",
          "transcript": "XM_047450099.1",
          "protein_id": "XP_047306055.1",
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          "cds_start": 8491,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047450099.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 59,
          "exon_rank_end": null,
          "exon_count": 63,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRYL",
          "gene_hgnc_id": 29127,
          "hgvs_c": "c.8446A>G",
          "hgvs_p": "p.Ile2816Val",
          "transcript": "XM_024453993.2",
          "protein_id": "XP_024309761.1",
          "transcript_support_level": null,
          "aa_start": 2816,
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          "cds_start": 8446,
          "cds_end": null,
          "cds_length": 9042,
          "cdna_start": null,
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        {
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          "gene_symbol": "FRYL",
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          "hgvs_c": "n.*4477A>G",
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          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000503238.6"
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      ],
      "gene_symbol": "FRYL",
      "gene_hgnc_id": 29127,
      "dbsnp": "rs1159981790",
      "frequency_reference_population": 6.8706515e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.87065e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.07244399189949036,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.10000000149011612,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.042,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0696,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.7,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.807,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.1,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_015030.2",
          "gene_symbol": "FRYL",
          "hgnc_id": 29127,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.8446A>G",
          "hgvs_p": "p.Ile2816Val"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}