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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-499374-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=499374&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 499374,
      "ref": "A",
      "alt": "G",
      "effect": "synonymous_variant",
      "transcript": "NM_001127178.3",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGG",
          "gene_hgnc_id": 25985,
          "hgvs_c": "c.39A>G",
          "hgvs_p": "p.Val13Val",
          "transcript": "NM_001127178.3",
          "protein_id": "NP_001120650.1",
          "transcript_support_level": null,
          "aa_start": 13,
          "aa_end": null,
          "aa_length": 983,
          "cds_start": 39,
          "cds_end": null,
          "cds_length": 2952,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000453061.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001127178.3"
        },
        {
          "aa_ref": "V",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGG",
          "gene_hgnc_id": 25985,
          "hgvs_c": "c.39A>G",
          "hgvs_p": "p.Val13Val",
          "transcript": "ENST00000453061.7",
          "protein_id": "ENSP00000415203.2",
          "transcript_support_level": 1,
          "aa_start": 13,
          "aa_end": null,
          "aa_length": 983,
          "cds_start": 39,
          "cds_end": null,
          "cds_length": 2952,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001127178.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000453061.7"
        },
        {
          "aa_ref": "V",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGG",
          "gene_hgnc_id": 25985,
          "hgvs_c": "c.39A>G",
          "hgvs_p": "p.Val13Val",
          "transcript": "ENST00000383028.8",
          "protein_id": "ENSP00000372494.4",
          "transcript_support_level": 1,
          "aa_start": 13,
          "aa_end": null,
          "aa_length": 850,
          "cds_start": 39,
          "cds_end": null,
          "cds_length": 2553,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000383028.8"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGG",
          "gene_hgnc_id": 25985,
          "hgvs_c": "c.-787A>G",
          "hgvs_p": null,
          "transcript": "ENST00000509768.1",
          "protein_id": "ENSP00000421550.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000509768.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGG",
          "gene_hgnc_id": 25985,
          "hgvs_c": "n.135A>G",
          "hgvs_p": null,
          "transcript": "ENST00000502311.1",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000502311.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGG",
          "gene_hgnc_id": 25985,
          "hgvs_c": "n.39A>G",
          "hgvs_p": null,
          "transcript": "ENST00000506402.5",
          "protein_id": "ENSP00000424619.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000506402.5"
        },
        {
          "aa_ref": "V",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGG",
          "gene_hgnc_id": 25985,
          "hgvs_c": "c.39A>G",
          "hgvs_p": "p.Val13Val",
          "transcript": "NM_017733.5",
          "protein_id": "NP_060203.3",
          "transcript_support_level": null,
          "aa_start": 13,
          "aa_end": null,
          "aa_length": 975,
          "cds_start": 39,
          "cds_end": null,
          "cds_length": 2928,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_017733.5"
        },
        {
          "aa_ref": "V",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGG",
          "gene_hgnc_id": 25985,
          "hgvs_c": "c.39A>G",
          "hgvs_p": "p.Val13Val",
          "transcript": "ENST00000310340.9",
          "protein_id": "ENSP00000311750.5",
          "transcript_support_level": 2,
          "aa_start": 13,
          "aa_end": null,
          "aa_length": 975,
          "cds_start": 39,
          "cds_end": null,
          "cds_length": 2928,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000310340.9"
        },
        {
          "aa_ref": "V",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGG",
          "gene_hgnc_id": 25985,
          "hgvs_c": "c.39A>G",
          "hgvs_p": "p.Val13Val",
          "transcript": "ENST00000868086.1",
          "protein_id": "ENSP00000538145.1",
          "transcript_support_level": null,
          "aa_start": 13,
          "aa_end": null,
          "aa_length": 913,
          "cds_start": 39,
          "cds_end": null,
          "cds_length": 2742,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000868086.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGG",
          "gene_hgnc_id": 25985,
          "hgvs_c": "c.39A>G",
          "hgvs_p": "p.Val13Val",
          "transcript": "ENST00000868087.1",
          "protein_id": "ENSP00000538146.1",
          "transcript_support_level": null,
          "aa_start": 13,
          "aa_end": null,
          "aa_length": 912,
          "cds_start": 39,
          "cds_end": null,
          "cds_length": 2739,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000868087.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGG",
          "gene_hgnc_id": 25985,
          "hgvs_c": "c.39A>G",
          "hgvs_p": "p.Val13Val",
          "transcript": "NM_001289052.2",
          "protein_id": "NP_001275981.1",
          "transcript_support_level": null,
          "aa_start": 13,
          "aa_end": null,
          "aa_length": 850,
          "cds_start": 39,
          "cds_end": null,
          "cds_length": 2553,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001289052.2"
        },
        {
          "aa_ref": "V",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGG",
          "gene_hgnc_id": 25985,
          "hgvs_c": "c.39A>G",
          "hgvs_p": "p.Val13Val",
          "transcript": "ENST00000936869.1",
          "protein_id": "ENSP00000606928.1",
          "transcript_support_level": null,
          "aa_start": 13,
          "aa_end": null,
          "aa_length": 664,
          "cds_start": 39,
          "cds_end": null,
          "cds_length": 1995,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000936869.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGG",
          "gene_hgnc_id": 25985,
          "hgvs_c": "c.39A>G",
          "hgvs_p": "p.Val13Val",
          "transcript": "NM_001345989.2",
          "protein_id": "NP_001332918.1",
          "transcript_support_level": null,
          "aa_start": 13,
          "aa_end": null,
          "aa_length": 612,
          "cds_start": 39,
          "cds_end": null,
          "cds_length": 1839,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001345989.2"
        },
        {
          "aa_ref": "V",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGG",
          "gene_hgnc_id": 25985,
          "hgvs_c": "c.39A>G",
          "hgvs_p": "p.Val13Val",
          "transcript": "XM_011513490.4",
          "protein_id": "XP_011511792.1",
          "transcript_support_level": null,
          "aa_start": 13,
          "aa_end": null,
          "aa_length": 930,
          "cds_start": 39,
          "cds_end": null,
          "cds_length": 2793,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011513490.4"
        },
        {
          "aa_ref": "V",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGG",
          "gene_hgnc_id": 25985,
          "hgvs_c": "c.39A>G",
          "hgvs_p": "p.Val13Val",
          "transcript": "XM_047415847.1",
          "protein_id": "XP_047271803.1",
          "transcript_support_level": null,
          "aa_start": 13,
          "aa_end": null,
          "aa_length": 701,
          "cds_start": 39,
          "cds_end": null,
          "cds_length": 2106,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047415847.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGG",
          "gene_hgnc_id": 25985,
          "hgvs_c": "c.39A>G",
          "hgvs_p": "p.Val13Val",
          "transcript": "XM_047415849.1",
          "protein_id": "XP_047271805.1",
          "transcript_support_level": null,
          "aa_start": 13,
          "aa_end": null,
          "aa_length": 624,
          "cds_start": 39,
          "cds_end": null,
          "cds_length": 1875,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047415849.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGG",
          "gene_hgnc_id": 25985,
          "hgvs_c": "c.39A>G",
          "hgvs_p": "p.Val13Val",
          "transcript": "XM_047415850.1",
          "protein_id": "XP_047271806.1",
          "transcript_support_level": null,
          "aa_start": 13,
          "aa_end": null,
          "aa_length": 619,
          "cds_start": 39,
          "cds_end": null,
          "cds_length": 1860,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047415850.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGG",
          "gene_hgnc_id": 25985,
          "hgvs_c": "c.39A>G",
          "hgvs_p": "p.Val13Val",
          "transcript": "XM_047415851.1",
          "protein_id": "XP_047271807.1",
          "transcript_support_level": null,
          "aa_start": 13,
          "aa_end": null,
          "aa_length": 611,
          "cds_start": 39,
          "cds_end": null,
          "cds_length": 1836,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047415851.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGG",
          "gene_hgnc_id": 25985,
          "hgvs_c": "c.39A>G",
          "hgvs_p": "p.Val13Val",
          "transcript": "XM_011513494.4",
          "protein_id": "XP_011511796.1",
          "transcript_support_level": null,
          "aa_start": 13,
          "aa_end": null,
          "aa_length": 547,
          "cds_start": 39,
          "cds_end": null,
          "cds_length": 1644,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011513494.4"
        },
        {
          "aa_ref": "V",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGG",
          "gene_hgnc_id": 25985,
          "hgvs_c": "c.39A>G",
          "hgvs_p": "p.Val13Val",
          "transcript": "XM_047415853.1",
          "protein_id": "XP_047271809.1",
          "transcript_support_level": null,
          "aa_start": 13,
          "aa_end": null,
          "aa_length": 539,
          "cds_start": 39,
          "cds_end": null,
          "cds_length": 1620,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047415853.1"
        },
        {
          "aa_ref": "V",
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      ],
      "gene_symbol": "PIGG",
      "gene_hgnc_id": 25985,
      "dbsnp": "rs1553874002",
      "frequency_reference_population": 0.0000020578445,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 3,
      "gnomad_exomes_af": 0.00000205784,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 3,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": -0.7400000095367432,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.74,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.696,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -5,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong,BP6_Moderate,BP7",
      "acmg_by_gene": [
        {
          "score": -5,
          "benign_score": 7,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong",
            "BP6_Moderate",
            "BP7"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001127178.3",
          "gene_symbol": "PIGG",
          "hgnc_id": 25985,
          "effects": [
            "synonymous_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.39A>G",
          "hgvs_p": "p.Val13Val"
        },
        {
          "score": -4,
          "benign_score": 6,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong",
            "BP6_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_133474.4",
          "gene_symbol": "ZNF721",
          "hgnc_id": 29425,
          "effects": [
            "upstream_gene_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.-412T>C",
          "hgvs_p": null
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      ],
      "clinvar_disease": " autosomal recessive 53,Intellectual disability",
      "clinvar_classification": "Likely benign",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LB:1",
      "phenotype_combined": "Intellectual disability, autosomal recessive 53",
      "pathogenicity_classification_combined": "Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}