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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-52072099-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=52072099&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 52072099,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_145263.4",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA18",
          "gene_hgnc_id": 29579,
          "hgvs_c": "c.701G>A",
          "hgvs_p": "p.Arg234Gln",
          "transcript": "NM_145263.4",
          "protein_id": "NP_660306.1",
          "transcript_support_level": null,
          "aa_start": 234,
          "aa_end": null,
          "aa_length": 538,
          "cds_start": 701,
          "cds_end": null,
          "cds_length": 1617,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000295213.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_145263.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA18",
          "gene_hgnc_id": 29579,
          "hgvs_c": "c.701G>A",
          "hgvs_p": "p.Arg234Gln",
          "transcript": "ENST00000295213.9",
          "protein_id": "ENSP00000295213.4",
          "transcript_support_level": 1,
          "aa_start": 234,
          "aa_end": null,
          "aa_length": 538,
          "cds_start": 701,
          "cds_end": null,
          "cds_length": 1617,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_145263.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000295213.9"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA18",
          "gene_hgnc_id": 29579,
          "hgvs_c": "c.605G>A",
          "hgvs_p": "p.Arg202Gln",
          "transcript": "NM_001297608.2",
          "protein_id": "NP_001284537.1",
          "transcript_support_level": null,
          "aa_start": 202,
          "aa_end": null,
          "aa_length": 506,
          "cds_start": 605,
          "cds_end": null,
          "cds_length": 1521,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001297608.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA18",
          "gene_hgnc_id": 29579,
          "hgvs_c": "c.605G>A",
          "hgvs_p": "p.Arg202Gln",
          "transcript": "ENST00000419395.6",
          "protein_id": "ENSP00000415309.2",
          "transcript_support_level": 2,
          "aa_start": 202,
          "aa_end": null,
          "aa_length": 506,
          "cds_start": 605,
          "cds_end": null,
          "cds_length": 1521,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000419395.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA18",
          "gene_hgnc_id": 29579,
          "hgvs_c": "c.701G>A",
          "hgvs_p": "p.Arg234Gln",
          "transcript": "ENST00000851879.1",
          "protein_id": "ENSP00000521938.1",
          "transcript_support_level": null,
          "aa_start": 234,
          "aa_end": null,
          "aa_length": 485,
          "cds_start": 701,
          "cds_end": null,
          "cds_length": 1458,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851879.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA18",
          "gene_hgnc_id": 29579,
          "hgvs_c": "c.482G>A",
          "hgvs_p": "p.Arg161Gln",
          "transcript": "ENST00000851876.1",
          "protein_id": "ENSP00000521935.1",
          "transcript_support_level": null,
          "aa_start": 161,
          "aa_end": null,
          "aa_length": 465,
          "cds_start": 482,
          "cds_end": null,
          "cds_length": 1398,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851876.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA18",
          "gene_hgnc_id": 29579,
          "hgvs_c": "c.605G>A",
          "hgvs_p": "p.Arg202Gln",
          "transcript": "ENST00000851878.1",
          "protein_id": "ENSP00000521937.1",
          "transcript_support_level": null,
          "aa_start": 202,
          "aa_end": null,
          "aa_length": 453,
          "cds_start": 605,
          "cds_end": null,
          "cds_length": 1362,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851878.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA18",
          "gene_hgnc_id": 29579,
          "hgvs_c": "c.386G>A",
          "hgvs_p": "p.Arg129Gln",
          "transcript": "ENST00000851874.1",
          "protein_id": "ENSP00000521933.1",
          "transcript_support_level": null,
          "aa_start": 129,
          "aa_end": null,
          "aa_length": 433,
          "cds_start": 386,
          "cds_end": null,
          "cds_length": 1302,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851874.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA18",
          "gene_hgnc_id": 29579,
          "hgvs_c": "c.290G>A",
          "hgvs_p": "p.Arg97Gln",
          "transcript": "NM_001346102.2",
          "protein_id": "NP_001333031.1",
          "transcript_support_level": null,
          "aa_start": 97,
          "aa_end": null,
          "aa_length": 401,
          "cds_start": 290,
          "cds_end": null,
          "cds_length": 1206,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001346102.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA18",
          "gene_hgnc_id": 29579,
          "hgvs_c": "c.701G>A",
          "hgvs_p": "p.Arg234Gln",
          "transcript": "ENST00000957036.1",
          "protein_id": "ENSP00000627095.1",
          "transcript_support_level": null,
          "aa_start": 234,
          "aa_end": null,
          "aa_length": 385,
          "cds_start": 701,
          "cds_end": null,
          "cds_length": 1158,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000957036.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA18",
          "gene_hgnc_id": 29579,
          "hgvs_c": "c.386G>A",
          "hgvs_p": "p.Arg129Gln",
          "transcript": "ENST00000957035.1",
          "protein_id": "ENSP00000627094.1",
          "transcript_support_level": null,
          "aa_start": 129,
          "aa_end": null,
          "aa_length": 380,
          "cds_start": 386,
          "cds_end": null,
          "cds_length": 1143,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000957035.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA18",
          "gene_hgnc_id": 29579,
          "hgvs_c": "c.26G>A",
          "hgvs_p": "p.Arg9Gln",
          "transcript": "NM_001346103.2",
          "protein_id": "NP_001333032.1",
          "transcript_support_level": null,
          "aa_start": 9,
          "aa_end": null,
          "aa_length": 313,
          "cds_start": 26,
          "cds_end": null,
          "cds_length": 942,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001346103.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA18",
          "gene_hgnc_id": 29579,
          "hgvs_c": "c.386G>A",
          "hgvs_p": "p.Arg129Gln",
          "transcript": "XM_047449604.1",
          "protein_id": "XP_047305560.1",
          "transcript_support_level": null,
          "aa_start": 129,
          "aa_end": null,
          "aa_length": 433,
          "cds_start": 386,
          "cds_end": null,
          "cds_length": 1302,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047449604.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA18",
          "gene_hgnc_id": 29579,
          "hgvs_c": "c.341G>A",
          "hgvs_p": "p.Arg114Gln",
          "transcript": "XM_017007731.3",
          "protein_id": "XP_016863220.1",
          "transcript_support_level": null,
          "aa_start": 114,
          "aa_end": null,
          "aa_length": 418,
          "cds_start": 341,
          "cds_end": null,
          "cds_length": 1257,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_017007731.3"
        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
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          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA18",
          "gene_hgnc_id": 29579,
          "hgvs_c": "c.311G>A",
          "hgvs_p": "p.Arg104Gln",
          "transcript": "XM_047449605.1",
          "protein_id": "XP_047305561.1",
          "transcript_support_level": null,
          "aa_start": 104,
          "aa_end": null,
          "aa_length": 408,
          "cds_start": 311,
          "cds_end": null,
          "cds_length": 1227,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047449605.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA18",
          "gene_hgnc_id": 29579,
          "hgvs_c": "c.701G>A",
          "hgvs_p": "p.Arg234Gln",
          "transcript": "XM_005265717.3",
          "protein_id": "XP_005265774.1",
          "transcript_support_level": null,
          "aa_start": 234,
          "aa_end": null,
          "aa_length": 357,
          "cds_start": 701,
          "cds_end": null,
          "cds_length": 1074,
          "cdna_start": null,
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          "cdna_length": null,
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          "biotype": "protein_coding",
          "feature": "XM_005265717.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA18",
          "gene_hgnc_id": 29579,
          "hgvs_c": "c.26G>A",
          "hgvs_p": "p.Arg9Gln",
          "transcript": "XM_011534372.2",
          "protein_id": "XP_011532674.1",
          "transcript_support_level": null,
          "aa_start": 9,
          "aa_end": null,
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          "cds_start": 26,
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          "biotype": "protein_coding",
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        },
        {
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          "canonical": false,
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          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
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          "exon_count": 11,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "SPATA18",
          "gene_hgnc_id": 29579,
          "hgvs_c": "c.423-4680G>A",
          "hgvs_p": null,
          "transcript": "ENST00000851873.1",
          "protein_id": "ENSP00000521932.1",
          "transcript_support_level": null,
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          "aa_length": 426,
          "cds_start": null,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000851873.1"
        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
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          "exon_count": 10,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "SPATA18",
          "gene_hgnc_id": 29579,
          "hgvs_c": "c.423-4680G>A",
          "hgvs_p": null,
          "transcript": "ENST00000928448.1",
          "protein_id": "ENSP00000598507.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": 398,
          "cds_start": null,
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          "cds_length": 1197,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928448.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "SPATA18",
          "gene_hgnc_id": 29579,
          "hgvs_c": "c.204-4680G>A",
          "hgvs_p": null,
          "transcript": "ENST00000851877.1",
          "protein_id": "ENSP00000521936.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": null,
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          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851877.1"
        },
        {
          "aa_ref": null,
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      ],
      "gene_symbol": "SPATA18",
      "gene_hgnc_id": 29579,
      "dbsnp": "rs139201868",
      "frequency_reference_population": 0.0035352898,
      "hom_count_reference_population": 17,
      "allele_count_reference_population": 5706,
      "gnomad_exomes_af": 0.00369047,
      "gnomad_genomes_af": 0.0020442,
      "gnomad_exomes_ac": 5395,
      "gnomad_genomes_ac": 311,
      "gnomad_exomes_homalt": 16,
      "gnomad_genomes_homalt": 1,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.0054242610931396484,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.053,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0626,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.44,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.26,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -16,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BS2",
      "acmg_by_gene": [
        {
          "score": -16,
          "benign_score": 16,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_145263.4",
          "gene_symbol": "SPATA18",
          "hgnc_id": 29579,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.701G>A",
          "hgvs_p": "p.Arg234Gln"
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      ],
      "clinvar_disease": "not provided",
      "clinvar_classification": "Likely benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LB:2",
      "phenotype_combined": "not provided",
      "pathogenicity_classification_combined": "Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}