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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-56011440-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=56011440&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 56011440,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_025009.5",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CEP135",
          "gene_hgnc_id": 29086,
          "hgvs_c": "c.2534T>C",
          "hgvs_p": "p.Val845Ala",
          "transcript": "NM_025009.5",
          "protein_id": "NP_079285.2",
          "transcript_support_level": null,
          "aa_start": 845,
          "aa_end": null,
          "aa_length": 1140,
          "cds_start": 2534,
          "cds_end": null,
          "cds_length": 3423,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000257287.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_025009.5"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CEP135",
          "gene_hgnc_id": 29086,
          "hgvs_c": "c.2534T>C",
          "hgvs_p": "p.Val845Ala",
          "transcript": "ENST00000257287.5",
          "protein_id": "ENSP00000257287.3",
          "transcript_support_level": 1,
          "aa_start": 845,
          "aa_end": null,
          "aa_length": 1140,
          "cds_start": 2534,
          "cds_end": null,
          "cds_length": 3423,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_025009.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000257287.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CEP135",
          "gene_hgnc_id": 29086,
          "hgvs_c": "n.2484T>C",
          "hgvs_p": null,
          "transcript": "ENST00000506202.1",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000506202.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CEP135",
          "gene_hgnc_id": 29086,
          "hgvs_c": "c.2534T>C",
          "hgvs_p": "p.Val845Ala",
          "transcript": "ENST00000916105.1",
          "protein_id": "ENSP00000586164.1",
          "transcript_support_level": null,
          "aa_start": 845,
          "aa_end": null,
          "aa_length": 1191,
          "cds_start": 2534,
          "cds_end": null,
          "cds_length": 3576,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000916105.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CEP135",
          "gene_hgnc_id": 29086,
          "hgvs_c": "c.2501T>C",
          "hgvs_p": "p.Val834Ala",
          "transcript": "ENST00000916104.1",
          "protein_id": "ENSP00000586163.1",
          "transcript_support_level": null,
          "aa_start": 834,
          "aa_end": null,
          "aa_length": 1129,
          "cds_start": 2501,
          "cds_end": null,
          "cds_length": 3390,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000916104.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CEP135",
          "gene_hgnc_id": 29086,
          "hgvs_c": "c.2468T>C",
          "hgvs_p": "p.Val823Ala",
          "transcript": "ENST00000916107.1",
          "protein_id": "ENSP00000586166.1",
          "transcript_support_level": null,
          "aa_start": 823,
          "aa_end": null,
          "aa_length": 1118,
          "cds_start": 2468,
          "cds_end": null,
          "cds_length": 3357,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000916107.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CEP135",
          "gene_hgnc_id": 29086,
          "hgvs_c": "c.2405T>C",
          "hgvs_p": "p.Val802Ala",
          "transcript": "ENST00000916106.1",
          "protein_id": "ENSP00000586165.1",
          "transcript_support_level": null,
          "aa_start": 802,
          "aa_end": null,
          "aa_length": 1097,
          "cds_start": 2405,
          "cds_end": null,
          "cds_length": 3294,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000916106.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CEP135",
          "gene_hgnc_id": 29086,
          "hgvs_c": "c.2501T>C",
          "hgvs_p": "p.Val834Ala",
          "transcript": "XM_006714055.4",
          "protein_id": "XP_006714118.1",
          "transcript_support_level": null,
          "aa_start": 834,
          "aa_end": null,
          "aa_length": 1129,
          "cds_start": 2501,
          "cds_end": null,
          "cds_length": 3390,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_006714055.4"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CEP135",
          "gene_hgnc_id": 29086,
          "hgvs_c": "c.1463T>C",
          "hgvs_p": "p.Val488Ala",
          "transcript": "XM_005265788.5",
          "protein_id": "XP_005265845.1",
          "transcript_support_level": null,
          "aa_start": 488,
          "aa_end": null,
          "aa_length": 783,
          "cds_start": 1463,
          "cds_end": null,
          "cds_length": 2352,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005265788.5"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CEP135",
          "gene_hgnc_id": 29086,
          "hgvs_c": "c.1004T>C",
          "hgvs_p": "p.Val335Ala",
          "transcript": "XM_011534412.2",
          "protein_id": "XP_011532714.1",
          "transcript_support_level": null,
          "aa_start": 335,
          "aa_end": null,
          "aa_length": 630,
          "cds_start": 1004,
          "cds_end": null,
          "cds_length": 1893,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011534412.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CEP135",
          "gene_hgnc_id": 29086,
          "hgvs_c": "n.599T>C",
          "hgvs_p": null,
          "transcript": "ENST00000706801.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000706801.1"
        }
      ],
      "gene_symbol": "CEP135",
      "gene_hgnc_id": 29086,
      "dbsnp": "rs141330867",
      "frequency_reference_population": 0.000060776172,
      "hom_count_reference_population": 1,
      "allele_count_reference_population": 98,
      "gnomad_exomes_af": 0.000054786,
      "gnomad_genomes_af": 0.00011823,
      "gnomad_exomes_ac": 80,
      "gnomad_genomes_ac": 18,
      "gnomad_exomes_homalt": 1,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.02574220299720764,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.083,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1715,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.54,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 6.598,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -5,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong,BP6",
      "acmg_by_gene": [
        {
          "score": -5,
          "benign_score": 5,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_025009.5",
          "gene_symbol": "CEP135",
          "hgnc_id": 29086,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2534T>C",
          "hgvs_p": "p.Val845Ala"
        }
      ],
      "clinvar_disease": " autosomal recessive, primary,Inborn genetic diseases,Microcephaly 8,not provided,not specified",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:3 LB:1",
      "phenotype_combined": "not specified|not provided|Microcephaly 8, primary, autosomal recessive|Inborn genetic diseases",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}