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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-663143-G-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=663143&ref=G&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 663143,
      "ref": "G",
      "alt": "T",
      "effect": "stop_gained",
      "transcript": "NM_001440547.1",
      "consequences": [
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE6B",
          "gene_hgnc_id": 8786,
          "hgvs_c": "c.1876G>T",
          "hgvs_p": "p.Glu626*",
          "transcript": "NM_000283.4",
          "protein_id": "NP_000274.3",
          "transcript_support_level": null,
          "aa_start": 626,
          "aa_end": null,
          "aa_length": 854,
          "cds_start": 1876,
          "cds_end": null,
          "cds_length": 2565,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000496514.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000283.4"
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE6B",
          "gene_hgnc_id": 8786,
          "hgvs_c": "c.1876G>T",
          "hgvs_p": "p.Glu626*",
          "transcript": "ENST00000496514.6",
          "protein_id": "ENSP00000420295.1",
          "transcript_support_level": 1,
          "aa_start": 626,
          "aa_end": null,
          "aa_length": 854,
          "cds_start": 1876,
          "cds_end": null,
          "cds_length": 2565,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_000283.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000496514.6"
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE6B",
          "gene_hgnc_id": 8786,
          "hgvs_c": "c.1876G>T",
          "hgvs_p": "p.Glu626*",
          "transcript": "ENST00000255622.10",
          "protein_id": "ENSP00000255622.6",
          "transcript_support_level": 1,
          "aa_start": 626,
          "aa_end": null,
          "aa_length": 853,
          "cds_start": 1876,
          "cds_end": null,
          "cds_length": 2562,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000255622.10"
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE6B",
          "gene_hgnc_id": 8786,
          "hgvs_c": "c.1876G>T",
          "hgvs_p": "p.Glu626*",
          "transcript": "NM_001440547.1",
          "protein_id": "NP_001427476.1",
          "transcript_support_level": null,
          "aa_start": 626,
          "aa_end": null,
          "aa_length": 868,
          "cds_start": 1876,
          "cds_end": null,
          "cds_length": 2607,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001440547.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE6B",
          "gene_hgnc_id": 8786,
          "hgvs_c": "c.1876G>T",
          "hgvs_p": "p.Glu626*",
          "transcript": "NM_001145291.2",
          "protein_id": "NP_001138763.2",
          "transcript_support_level": null,
          "aa_start": 626,
          "aa_end": null,
          "aa_length": 853,
          "cds_start": 1876,
          "cds_end": null,
          "cds_length": 2562,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001145291.2"
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE6B",
          "gene_hgnc_id": 8786,
          "hgvs_c": "c.1876G>T",
          "hgvs_p": "p.Glu626*",
          "transcript": "NM_001440548.1",
          "protein_id": "NP_001427477.1",
          "transcript_support_level": null,
          "aa_start": 626,
          "aa_end": null,
          "aa_length": 784,
          "cds_start": 1876,
          "cds_end": null,
          "cds_length": 2355,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001440548.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE6B",
          "gene_hgnc_id": 8786,
          "hgvs_c": "c.1039G>T",
          "hgvs_p": "p.Glu347*",
          "transcript": "NM_001350154.3",
          "protein_id": "NP_001337083.1",
          "transcript_support_level": null,
          "aa_start": 347,
          "aa_end": null,
          "aa_length": 589,
          "cds_start": 1039,
          "cds_end": null,
          "cds_length": 1770,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001350154.3"
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE6B",
          "gene_hgnc_id": 8786,
          "hgvs_c": "c.1039G>T",
          "hgvs_p": "p.Glu347*",
          "transcript": "NM_001145292.2",
          "protein_id": "NP_001138764.2",
          "transcript_support_level": null,
          "aa_start": 347,
          "aa_end": null,
          "aa_length": 575,
          "cds_start": 1039,
          "cds_end": null,
          "cds_length": 1728,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001145292.2"
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE6B",
          "gene_hgnc_id": 8786,
          "hgvs_c": "c.1039G>T",
          "hgvs_p": "p.Glu347*",
          "transcript": "NM_001379246.1",
          "protein_id": "NP_001366175.1",
          "transcript_support_level": null,
          "aa_start": 347,
          "aa_end": null,
          "aa_length": 575,
          "cds_start": 1039,
          "cds_end": null,
          "cds_length": 1728,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001379246.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE6B",
          "gene_hgnc_id": 8786,
          "hgvs_c": "c.1039G>T",
          "hgvs_p": "p.Glu347*",
          "transcript": "NM_001379247.1",
          "protein_id": "NP_001366176.1",
          "transcript_support_level": null,
          "aa_start": 347,
          "aa_end": null,
          "aa_length": 575,
          "cds_start": 1039,
          "cds_end": null,
          "cds_length": 1728,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001379247.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE6B",
          "gene_hgnc_id": 8786,
          "hgvs_c": "c.1039G>T",
          "hgvs_p": "p.Glu347*",
          "transcript": "ENST00000429163.6",
          "protein_id": "ENSP00000406334.2",
          "transcript_support_level": 2,
          "aa_start": 347,
          "aa_end": null,
          "aa_length": 575,
          "cds_start": 1039,
          "cds_end": null,
          "cds_length": 1728,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000429163.6"
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE6B",
          "gene_hgnc_id": 8786,
          "hgvs_c": "c.721G>T",
          "hgvs_p": "p.Glu241*",
          "transcript": "NM_001350155.3",
          "protein_id": "NP_001337084.1",
          "transcript_support_level": null,
          "aa_start": 241,
          "aa_end": null,
          "aa_length": 483,
          "cds_start": 721,
          "cds_end": null,
          "cds_length": 1452,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001350155.3"
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE6B",
          "gene_hgnc_id": 8786,
          "hgvs_c": "c.1876G>T",
          "hgvs_p": "p.Glu626*",
          "transcript": "XM_047415772.1",
          "protein_id": "XP_047271728.1",
          "transcript_support_level": null,
          "aa_start": 626,
          "aa_end": null,
          "aa_length": 949,
          "cds_start": 1876,
          "cds_end": null,
          "cds_length": 2850,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047415772.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE6B",
          "gene_hgnc_id": 8786,
          "hgvs_c": "c.1876G>T",
          "hgvs_p": "p.Glu626*",
          "transcript": "XM_047415773.1",
          "protein_id": "XP_047271729.1",
          "transcript_support_level": null,
          "aa_start": 626,
          "aa_end": null,
          "aa_length": 935,
          "cds_start": 1876,
          "cds_end": null,
          "cds_length": 2808,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047415773.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE6B",
          "gene_hgnc_id": 8786,
          "hgvs_c": "c.1039G>T",
          "hgvs_p": "p.Glu347*",
          "transcript": "XM_047415775.1",
          "protein_id": "XP_047271731.1",
          "transcript_support_level": null,
          "aa_start": 347,
          "aa_end": null,
          "aa_length": 670,
          "cds_start": 1039,
          "cds_end": null,
          "cds_length": 2013,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047415775.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE6B",
          "gene_hgnc_id": 8786,
          "hgvs_c": "c.1039G>T",
          "hgvs_p": "p.Glu347*",
          "transcript": "XM_047415776.1",
          "protein_id": "XP_047271732.1",
          "transcript_support_level": null,
          "aa_start": 347,
          "aa_end": null,
          "aa_length": 670,
          "cds_start": 1039,
          "cds_end": null,
          "cds_length": 2013,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047415776.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PDE6B",
          "gene_hgnc_id": 8786,
          "hgvs_c": "c.1039G>T",
          "hgvs_p": "p.Glu347*",
          "transcript": "XM_047415777.1",
          "protein_id": "XP_047271733.1",
          "transcript_support_level": null,
          "aa_start": 347,
          "aa_end": null,
          "aa_length": 670,
          "cds_start": 1039,
          "cds_end": null,
          "cds_length": 2013,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047415777.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000272927",
          "gene_hgnc_id": 58704,
          "hgvs_c": "n.236+59C>A",
          "hgvs_p": null,
          "transcript": "ENST00000609172.2",
          "protein_id": null,
          "transcript_support_level": 6,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000609172.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000272927",
          "gene_hgnc_id": 58704,
          "hgvs_c": "n.233+59C>A",
          "hgvs_p": null,
          "transcript": "ENST00000784562.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000784562.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000272927",
          "gene_hgnc_id": 58704,
          "hgvs_c": "n.303+59C>A",
          "hgvs_p": null,
          "transcript": "ENST00000784563.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000784563.1"
        }
      ],
      "gene_symbol": "PDE6B",
      "gene_hgnc_id": 8786,
      "dbsnp": "rs758052437",
      "frequency_reference_population": 6.8475515e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84755e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.6499999761581421,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0.7400000095367432,
      "splice_prediction_selected": "Pathogenic",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.65,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 9.169,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.74,
      "spliceai_max_prediction": "Pathogenic",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 12,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PVS1,PM2,PP3,PP5",
      "acmg_by_gene": [
        {
          "score": 12,
          "benign_score": 0,
          "pathogenic_score": 12,
          "criteria": [
            "PVS1",
            "PM2",
            "PP3",
            "PP5"
          ],
          "verdict": "Pathogenic",
          "transcript": "NM_001440547.1",
          "gene_symbol": "PDE6B",
          "hgnc_id": 8786,
          "effects": [
            "stop_gained"
          ],
          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.1876G>T",
          "hgvs_p": "p.Glu626*"
        },
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000784562.1",
          "gene_symbol": "ENSG00000272927",
          "hgnc_id": 58704,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.233+59C>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Retinitis pigmentosa 40",
      "clinvar_classification": "Likely pathogenic",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "Retinitis pigmentosa 40",
      "pathogenicity_classification_combined": "Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}