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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-70783179-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=70783179&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 70783179,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_001037442.4",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUFY3",
          "gene_hgnc_id": 30285,
          "hgvs_c": "c.983G>A",
          "hgvs_p": "p.Arg328Gln",
          "transcript": "NM_001037442.4",
          "protein_id": "NP_001032519.1",
          "transcript_support_level": null,
          "aa_start": 328,
          "aa_end": null,
          "aa_length": 620,
          "cds_start": 983,
          "cds_end": null,
          "cds_length": 1863,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000381006.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001037442.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUFY3",
          "gene_hgnc_id": 30285,
          "hgvs_c": "c.983G>A",
          "hgvs_p": "p.Arg328Gln",
          "transcript": "ENST00000381006.8",
          "protein_id": "ENSP00000370394.3",
          "transcript_support_level": 5,
          "aa_start": 328,
          "aa_end": null,
          "aa_length": 620,
          "cds_start": 983,
          "cds_end": null,
          "cds_length": 1863,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001037442.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000381006.8"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUFY3",
          "gene_hgnc_id": 30285,
          "hgvs_c": "c.1163G>A",
          "hgvs_p": "p.Arg388Gln",
          "transcript": "ENST00000417478.6",
          "protein_id": "ENSP00000399771.2",
          "transcript_support_level": 1,
          "aa_start": 388,
          "aa_end": null,
          "aa_length": 506,
          "cds_start": 1163,
          "cds_end": null,
          "cds_length": 1521,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000417478.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUFY3",
          "gene_hgnc_id": 30285,
          "hgvs_c": "c.983G>A",
          "hgvs_p": "p.Arg328Gln",
          "transcript": "ENST00000226328.8",
          "protein_id": "ENSP00000226328.4",
          "transcript_support_level": 1,
          "aa_start": 328,
          "aa_end": null,
          "aa_length": 469,
          "cds_start": 983,
          "cds_end": null,
          "cds_length": 1410,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000226328.8"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUFY3",
          "gene_hgnc_id": 30285,
          "hgvs_c": "c.983G>A",
          "hgvs_p": "p.Arg328Gln",
          "transcript": "ENST00000947599.1",
          "protein_id": "ENSP00000617658.1",
          "transcript_support_level": null,
          "aa_start": 328,
          "aa_end": null,
          "aa_length": 620,
          "cds_start": 983,
          "cds_end": null,
          "cds_length": 1863,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000947599.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUFY3",
          "gene_hgnc_id": 30285,
          "hgvs_c": "c.824G>A",
          "hgvs_p": "p.Arg275Gln",
          "transcript": "NM_001291993.2",
          "protein_id": "NP_001278922.1",
          "transcript_support_level": null,
          "aa_start": 275,
          "aa_end": null,
          "aa_length": 567,
          "cds_start": 824,
          "cds_end": null,
          "cds_length": 1704,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001291993.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUFY3",
          "gene_hgnc_id": 30285,
          "hgvs_c": "c.824G>A",
          "hgvs_p": "p.Arg275Gln",
          "transcript": "ENST00000502653.5",
          "protein_id": "ENSP00000425400.1",
          "transcript_support_level": 2,
          "aa_start": 275,
          "aa_end": null,
          "aa_length": 567,
          "cds_start": 824,
          "cds_end": null,
          "cds_length": 1704,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000502653.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUFY3",
          "gene_hgnc_id": 30285,
          "hgvs_c": "c.1163G>A",
          "hgvs_p": "p.Arg388Gln",
          "transcript": "NM_001130709.2",
          "protein_id": "NP_001124181.1",
          "transcript_support_level": null,
          "aa_start": 388,
          "aa_end": null,
          "aa_length": 506,
          "cds_start": 1163,
          "cds_end": null,
          "cds_length": 1521,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001130709.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUFY3",
          "gene_hgnc_id": 30285,
          "hgvs_c": "c.1037G>A",
          "hgvs_p": "p.Arg346Gln",
          "transcript": "NM_001291994.2",
          "protein_id": "NP_001278923.1",
          "transcript_support_level": null,
          "aa_start": 346,
          "aa_end": null,
          "aa_length": 487,
          "cds_start": 1037,
          "cds_end": null,
          "cds_length": 1464,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001291994.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUFY3",
          "gene_hgnc_id": 30285,
          "hgvs_c": "c.983G>A",
          "hgvs_p": "p.Arg328Gln",
          "transcript": "NM_014961.5",
          "protein_id": "NP_055776.1",
          "transcript_support_level": null,
          "aa_start": 328,
          "aa_end": null,
          "aa_length": 469,
          "cds_start": 983,
          "cds_end": null,
          "cds_length": 1410,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_014961.5"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUFY3",
          "gene_hgnc_id": 30285,
          "hgvs_c": "c.824G>A",
          "hgvs_p": "p.Arg275Gln",
          "transcript": "NM_001345840.2",
          "protein_id": "NP_001332769.1",
          "transcript_support_level": null,
          "aa_start": 275,
          "aa_end": null,
          "aa_length": 416,
          "cds_start": 824,
          "cds_end": null,
          "cds_length": 1251,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001345840.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUFY3",
          "gene_hgnc_id": 30285,
          "hgvs_c": "c.1163G>A",
          "hgvs_p": "p.Arg388Gln",
          "transcript": "XM_011531750.3",
          "protein_id": "XP_011530052.1",
          "transcript_support_level": null,
          "aa_start": 388,
          "aa_end": null,
          "aa_length": 680,
          "cds_start": 1163,
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          "cds_length": 2043,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_011531750.3"
        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
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          "gene_symbol": "RUFY3",
          "gene_hgnc_id": 30285,
          "hgvs_c": "c.1163G>A",
          "hgvs_p": "p.Arg388Gln",
          "transcript": "XM_047449825.1",
          "protein_id": "XP_047305781.1",
          "transcript_support_level": null,
          "aa_start": 388,
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          "cds_start": 1163,
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          "cds_length": 2043,
          "cdna_start": null,
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          "cdna_length": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUFY3",
          "gene_hgnc_id": 30285,
          "hgvs_c": "c.1163G>A",
          "hgvs_p": "p.Arg388Gln",
          "transcript": "XM_011531751.4",
          "protein_id": "XP_011530053.1",
          "transcript_support_level": null,
          "aa_start": 388,
          "aa_end": null,
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          "cds_start": 1163,
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        {
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          ],
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          "intron_rank": null,
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          "gene_symbol": "RUFY3",
          "gene_hgnc_id": 30285,
          "hgvs_c": "c.1037G>A",
          "hgvs_p": "p.Arg346Gln",
          "transcript": "XM_005265656.4",
          "protein_id": "XP_005265713.1",
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          "aa_end": null,
          "aa_length": 638,
          "cds_start": 1037,
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          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "XM_005265656.4"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
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          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUFY3",
          "gene_hgnc_id": 30285,
          "hgvs_c": "c.983G>A",
          "hgvs_p": "p.Arg328Gln",
          "transcript": "XM_047449826.1",
          "protein_id": "XP_047305782.1",
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          "aa_start": 328,
          "aa_end": null,
          "aa_length": 597,
          "cds_start": 983,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
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          "gene_symbol": "RUFY3",
          "gene_hgnc_id": 30285,
          "hgvs_c": "c.1163G>A",
          "hgvs_p": "p.Arg388Gln",
          "transcript": "XM_047449827.1",
          "protein_id": "XP_047305783.1",
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        {
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          "exon_count": 14,
          "intron_rank": null,
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          "gene_symbol": "RUFY3",
          "gene_hgnc_id": 30285,
          "hgvs_c": "c.1163G>A",
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          "transcript": "XM_047449828.1",
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          "cds_start": 1163,
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        },
        {
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          ],
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          "intron_rank": null,
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          "gene_symbol": "RUFY3",
          "gene_hgnc_id": 30285,
          "hgvs_c": "c.791G>A",
          "hgvs_p": "p.Arg264Gln",
          "transcript": "XM_005265658.3",
          "protein_id": "XP_005265715.1",
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          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "XM_005265658.3"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUFY3",
          "gene_hgnc_id": 30285,
          "hgvs_c": "c.1163G>A",
          "hgvs_p": "p.Arg388Gln",
          "transcript": "XM_011531755.4",
          "protein_id": "XP_011530057.1",
          "transcript_support_level": null,
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          "cds_start": 1163,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "hgvs_c": "c.1037G>A",
          "hgvs_p": "p.Arg346Gln",
          "transcript": "XM_047449829.1",
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          "cds_start": 1037,
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        {
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          "gene_symbol": "RUFY3",
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          "hgvs_c": "c.983G>A",
          "hgvs_p": "p.Arg328Gln",
          "transcript": "XM_047449830.1",
          "protein_id": "XP_047305786.1",
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          "cds_start": 983,
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          "cds_length": 1494,
          "cdna_start": null,
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        {
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          "protein_coding": false,
          "strand": true,
          "consequences": [
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          "exon_count": 16,
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          "gene_symbol": "RUFY3",
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          "hgvs_c": "n.1361G>A",
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          "biotype": "pseudogene",
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        {
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          "protein_coding": false,
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          "consequences": [
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          "intron_rank": null,
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          "gene_symbol": "RUFY3",
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          "hgvs_c": "n.1361G>A",
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          "transcript": "XR_007096385.1",
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          "transcript_support_level": null,
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          "cdna_start": null,
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          "cdna_length": null,
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          "biotype": "pseudogene",
          "feature": "XR_007096385.1"
        },
        {
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          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
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          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUFY3",
          "gene_hgnc_id": 30285,
          "hgvs_c": "n.1361G>A",
          "hgvs_p": null,
          "transcript": "XR_938709.4",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
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          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "XR_938709.4"
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      ],
      "gene_symbol": "RUFY3",
      "gene_hgnc_id": 30285,
      "dbsnp": "rs758649386",
      "frequency_reference_population": 0.000029224051,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 46,
      "gnomad_exomes_af": 0.0000274284,
      "gnomad_genomes_af": 0.000046003,
      "gnomad_exomes_ac": 39,
      "gnomad_genomes_ac": 7,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.22642576694488525,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.254,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0768,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.17,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 7.808,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001037442.4",
          "gene_symbol": "RUFY3",
          "hgnc_id": 30285,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.983G>A",
          "hgvs_p": "p.Arg328Gln"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}