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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-76256355-A-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=76256355&ref=A&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 76256355,
      "ref": "A",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_001136570.3",
      "consequences": [
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAM47E",
          "gene_hgnc_id": 34343,
          "hgvs_c": "c.252A>C",
          "hgvs_p": "p.Gln84His",
          "transcript": "NM_001136570.3",
          "protein_id": "NP_001130042.1",
          "transcript_support_level": null,
          "aa_start": 84,
          "aa_end": null,
          "aa_length": 393,
          "cds_start": 252,
          "cds_end": null,
          "cds_length": 1182,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000424749.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001136570.3"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAM47E",
          "gene_hgnc_id": 34343,
          "hgvs_c": "c.252A>C",
          "hgvs_p": "p.Gln84His",
          "transcript": "ENST00000424749.7",
          "protein_id": "ENSP00000409423.2",
          "transcript_support_level": 5,
          "aa_start": 84,
          "aa_end": null,
          "aa_length": 393,
          "cds_start": 252,
          "cds_end": null,
          "cds_length": 1182,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001136570.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000424749.7"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAM47E-STBD1",
          "gene_hgnc_id": 44667,
          "hgvs_c": "c.252A>C",
          "hgvs_p": "p.Gln84His",
          "transcript": "ENST00000515604.5",
          "protein_id": "ENSP00000422067.1",
          "transcript_support_level": 2,
          "aa_start": 84,
          "aa_end": null,
          "aa_length": 351,
          "cds_start": 252,
          "cds_end": null,
          "cds_length": 1056,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000515604.5"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAM47E",
          "gene_hgnc_id": 34343,
          "hgvs_c": "c.252A>C",
          "hgvs_p": "p.Gln84His",
          "transcript": "ENST00000853410.1",
          "protein_id": "ENSP00000523469.1",
          "transcript_support_level": null,
          "aa_start": 84,
          "aa_end": null,
          "aa_length": 408,
          "cds_start": 252,
          "cds_end": null,
          "cds_length": 1227,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000853410.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAM47E-STBD1",
          "gene_hgnc_id": 44667,
          "hgvs_c": "c.252A>C",
          "hgvs_p": "p.Gln84His",
          "transcript": "NM_001242939.2",
          "protein_id": "NP_001229868.1",
          "transcript_support_level": null,
          "aa_start": 84,
          "aa_end": null,
          "aa_length": 351,
          "cds_start": 252,
          "cds_end": null,
          "cds_length": 1056,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001242939.2"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAM47E",
          "gene_hgnc_id": 34343,
          "hgvs_c": "c.252A>C",
          "hgvs_p": "p.Gln84His",
          "transcript": "ENST00000946092.1",
          "protein_id": "ENSP00000616151.1",
          "transcript_support_level": null,
          "aa_start": 84,
          "aa_end": null,
          "aa_length": 341,
          "cds_start": 252,
          "cds_end": null,
          "cds_length": 1026,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946092.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAM47E",
          "gene_hgnc_id": 34343,
          "hgvs_c": "c.252A>C",
          "hgvs_p": "p.Gln84His",
          "transcript": "ENST00000946090.1",
          "protein_id": "ENSP00000616149.1",
          "transcript_support_level": null,
          "aa_start": 84,
          "aa_end": null,
          "aa_length": 326,
          "cds_start": 252,
          "cds_end": null,
          "cds_length": 981,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946090.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAM47E",
          "gene_hgnc_id": 34343,
          "hgvs_c": "c.252A>C",
          "hgvs_p": "p.Gln84His",
          "transcript": "ENST00000853411.1",
          "protein_id": "ENSP00000523470.1",
          "transcript_support_level": null,
          "aa_start": 84,
          "aa_end": null,
          "aa_length": 315,
          "cds_start": 252,
          "cds_end": null,
          "cds_length": 948,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000853411.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAM47E",
          "gene_hgnc_id": 34343,
          "hgvs_c": "c.252A>C",
          "hgvs_p": "p.Gln84His",
          "transcript": "ENST00000946091.1",
          "protein_id": "ENSP00000616150.1",
          "transcript_support_level": null,
          "aa_start": 84,
          "aa_end": null,
          "aa_length": 274,
          "cds_start": 252,
          "cds_end": null,
          "cds_length": 825,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946091.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "FAM47E",
          "gene_hgnc_id": 34343,
          "hgvs_c": "c.82-7349A>C",
          "hgvs_p": null,
          "transcript": "NM_001242936.1",
          "protein_id": "NP_001229865.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 295,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 888,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001242936.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "FAM47E",
          "gene_hgnc_id": 34343,
          "hgvs_c": "c.82-7349A>C",
          "hgvs_p": null,
          "transcript": "ENST00000510197.5",
          "protein_id": "ENSP00000422262.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 295,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 888,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000510197.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "FAM47E",
          "gene_hgnc_id": 34343,
          "hgvs_c": "c.-85+4535A>C",
          "hgvs_p": null,
          "transcript": "ENST00000502320.2",
          "protein_id": "ENSP00000490159.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 181,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 546,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000502320.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "FAM47E",
          "gene_hgnc_id": 34343,
          "hgvs_c": "c.151-7349A>C",
          "hgvs_p": null,
          "transcript": "ENST00000512895.5",
          "protein_id": "ENSP00000422559.1",
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 132,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 400,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000512895.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "FAM47E",
          "gene_hgnc_id": 34343,
          "hgvs_c": "c.-85+4535A>C",
          "hgvs_p": null,
          "transcript": "ENST00000510328.5",
          "protein_id": "ENSP00000489801.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 129,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 390,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000510328.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAM47E",
          "gene_hgnc_id": 34343,
          "hgvs_c": "n.562A>C",
          "hgvs_p": null,
          "transcript": "ENST00000515589.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000515589.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "FAM47E-STBD1",
          "gene_hgnc_id": 44667,
          "hgvs_c": "n.74+4535A>C",
          "hgvs_p": null,
          "transcript": "ENST00000509377.1",
          "protein_id": "ENSP00000425528.2",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000509377.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "FAM47E-STBD1",
          "gene_hgnc_id": 44667,
          "hgvs_c": "n.74+4535A>C",
          "hgvs_p": null,
          "transcript": "ENST00000514365.5",
          "protein_id": "ENSP00000424458.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000514365.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "FAM47E-STBD1",
          "gene_hgnc_id": 44667,
          "hgvs_c": "n.74+4535A>C",
          "hgvs_p": null,
          "transcript": "ENST00000651133.1",
          "protein_id": "ENSP00000498232.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000651133.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000287401",
          "gene_hgnc_id": null,
          "hgvs_c": "n.554+580T>G",
          "hgvs_p": null,
          "transcript": "ENST00000670253.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000670253.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FAM47E",
          "gene_hgnc_id": 34343,
          "hgvs_c": "c.-100A>C",
          "hgvs_p": null,
          "transcript": "ENST00000606246.1",
          "protein_id": "ENSP00000476166.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 123,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 372,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000606246.1"
        }
      ],
      "gene_symbol": "FAM47E",
      "gene_hgnc_id": 34343,
      "dbsnp": "rs774362095",
      "frequency_reference_population": 0.00008958622,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 139,
      "gnomad_exomes_af": 0.0000914631,
      "gnomad_genomes_af": 0.000072318,
      "gnomad_exomes_ac": 128,
      "gnomad_genomes_ac": 11,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.05183756351470947,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.024,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1571,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.75,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -2.567,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001136570.3",
          "gene_symbol": "FAM47E",
          "hgnc_id": 34343,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.252A>C",
          "hgvs_p": "p.Gln84His"
        },
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001242939.2",
          "gene_symbol": "FAM47E-STBD1",
          "hgnc_id": 44667,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.252A>C",
          "hgvs_p": "p.Gln84His"
        },
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000670253.1",
          "gene_symbol": "ENSG00000287401",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.554+580T>G",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}