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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-85994853-C-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=85994853&ref=C&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 85994853,
      "ref": "C",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_001025616.3",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGAP24",
          "gene_hgnc_id": 25361,
          "hgvs_c": "c.1199C>A",
          "hgvs_p": "p.Thr400Asn",
          "transcript": "NM_001025616.3",
          "protein_id": "NP_001020787.2",
          "transcript_support_level": null,
          "aa_start": 400,
          "aa_end": null,
          "aa_length": 748,
          "cds_start": 1199,
          "cds_end": null,
          "cds_length": 2247,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000395184.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001025616.3"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGAP24",
          "gene_hgnc_id": 25361,
          "hgvs_c": "c.1199C>A",
          "hgvs_p": "p.Thr400Asn",
          "transcript": "ENST00000395184.6",
          "protein_id": "ENSP00000378611.1",
          "transcript_support_level": 2,
          "aa_start": 400,
          "aa_end": null,
          "aa_length": 748,
          "cds_start": 1199,
          "cds_end": null,
          "cds_length": 2247,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001025616.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000395184.6"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGAP24",
          "gene_hgnc_id": 25361,
          "hgvs_c": "c.920C>A",
          "hgvs_p": "p.Thr307Asn",
          "transcript": "ENST00000264343.4",
          "protein_id": "ENSP00000264343.4",
          "transcript_support_level": 1,
          "aa_start": 307,
          "aa_end": null,
          "aa_length": 655,
          "cds_start": 920,
          "cds_end": null,
          "cds_length": 1968,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000264343.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGAP24",
          "gene_hgnc_id": 25361,
          "hgvs_c": "c.914C>A",
          "hgvs_p": "p.Thr305Asn",
          "transcript": "ENST00000395183.6",
          "protein_id": "ENSP00000378610.2",
          "transcript_support_level": 1,
          "aa_start": 305,
          "aa_end": null,
          "aa_length": 653,
          "cds_start": 914,
          "cds_end": null,
          "cds_length": 1962,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000395183.6"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGAP24",
          "gene_hgnc_id": 25361,
          "hgvs_c": "c.944C>A",
          "hgvs_p": "p.Thr315Asn",
          "transcript": "ENST00000514229.5",
          "protein_id": "ENSP00000425589.1",
          "transcript_support_level": 1,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 621,
          "cds_start": 944,
          "cds_end": null,
          "cds_length": 1866,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000514229.5"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGAP24",
          "gene_hgnc_id": 25361,
          "hgvs_c": "c.944C>A",
          "hgvs_p": "p.Thr315Asn",
          "transcript": "NM_001287805.2",
          "protein_id": "NP_001274734.1",
          "transcript_support_level": null,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 663,
          "cds_start": 944,
          "cds_end": null,
          "cds_length": 1992,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001287805.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGAP24",
          "gene_hgnc_id": 25361,
          "hgvs_c": "c.920C>A",
          "hgvs_p": "p.Thr307Asn",
          "transcript": "NM_031305.3",
          "protein_id": "NP_112595.2",
          "transcript_support_level": null,
          "aa_start": 307,
          "aa_end": null,
          "aa_length": 655,
          "cds_start": 920,
          "cds_end": null,
          "cds_length": 1968,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_031305.3"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGAP24",
          "gene_hgnc_id": 25361,
          "hgvs_c": "c.914C>A",
          "hgvs_p": "p.Thr305Asn",
          "transcript": "NM_001042669.2",
          "protein_id": "NP_001036134.1",
          "transcript_support_level": null,
          "aa_start": 305,
          "aa_end": null,
          "aa_length": 653,
          "cds_start": 914,
          "cds_end": null,
          "cds_length": 1962,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001042669.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGAP24",
          "gene_hgnc_id": 25361,
          "hgvs_c": "c.620C>A",
          "hgvs_p": "p.Thr207Asn",
          "transcript": "NM_001346093.2",
          "protein_id": "NP_001333022.1",
          "transcript_support_level": null,
          "aa_start": 207,
          "aa_end": null,
          "aa_length": 555,
          "cds_start": 620,
          "cds_end": null,
          "cds_length": 1668,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001346093.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGAP24",
          "gene_hgnc_id": 25361,
          "hgvs_c": "c.914C>A",
          "hgvs_p": "p.Thr305Asn",
          "transcript": "XM_011532300.3",
          "protein_id": "XP_011530602.1",
          "transcript_support_level": null,
          "aa_start": 305,
          "aa_end": null,
          "aa_length": 653,
          "cds_start": 914,
          "cds_end": null,
          "cds_length": 1962,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011532300.3"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGAP24",
          "gene_hgnc_id": 25361,
          "hgvs_c": "c.914C>A",
          "hgvs_p": "p.Thr305Asn",
          "transcript": "XM_024454238.2",
          "protein_id": "XP_024310006.1",
          "transcript_support_level": null,
          "aa_start": 305,
          "aa_end": null,
          "aa_length": 653,
          "cds_start": 914,
          "cds_end": null,
          "cds_length": 1962,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_024454238.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGAP24",
          "gene_hgnc_id": 25361,
          "hgvs_c": "c.914C>A",
          "hgvs_p": "p.Thr305Asn",
          "transcript": "XM_047416235.1",
          "protein_id": "XP_047272191.1",
          "transcript_support_level": null,
          "aa_start": 305,
          "aa_end": null,
          "aa_length": 653,
          "cds_start": 914,
          "cds_end": null,
          "cds_length": 1962,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047416235.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "MAPK10",
          "gene_hgnc_id": 6872,
          "hgvs_c": "c.1392-2690G>T",
          "hgvs_p": null,
          "transcript": "ENST00000641010.1",
          "protein_id": "ENSP00000493178.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 473,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1422,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000641010.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "MAPK10",
          "gene_hgnc_id": 6872,
          "hgvs_c": "c.1278-2690G>T",
          "hgvs_p": null,
          "transcript": "ENST00000641324.1",
          "protein_id": "ENSP00000492888.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 435,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1308,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000641324.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "MAPK10",
          "gene_hgnc_id": 6872,
          "hgvs_c": "n.176-2690G>T",
          "hgvs_p": null,
          "transcript": "ENST00000641372.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000641372.1"
        }
      ],
      "gene_symbol": "ARHGAP24",
      "gene_hgnc_id": 25361,
      "dbsnp": "rs1283015218",
      "frequency_reference_population": 0.000003097882,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 5,
      "gnomad_exomes_af": 0.00000273619,
      "gnomad_genomes_af": 0.00000657384,
      "gnomad_exomes_ac": 4,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.18272453546524048,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.068,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0747,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.62,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 5.804,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001025616.3",
          "gene_symbol": "ARHGAP24",
          "hgnc_id": 25361,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.1199C>A",
          "hgvs_p": "p.Thr400Asn"
        },
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000641010.1",
          "gene_symbol": "MAPK10",
          "hgnc_id": 6872,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.1392-2690G>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not provided",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}