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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-87977065-C-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=87977065&ref=C&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 87977065,
      "ref": "C",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_001040058.2",
      "consequences": [
        {
          "aa_ref": "Q",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPP1",
          "gene_hgnc_id": 11255,
          "hgvs_c": "c.61C>A",
          "hgvs_p": "p.Gln21Lys",
          "transcript": "NM_001040058.2",
          "protein_id": "NP_001035147.1",
          "transcript_support_level": null,
          "aa_start": 21,
          "aa_end": null,
          "aa_length": 314,
          "cds_start": 61,
          "cds_end": null,
          "cds_length": 945,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000395080.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001040058.2"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "K",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPP1",
          "gene_hgnc_id": 11255,
          "hgvs_c": "c.61C>A",
          "hgvs_p": "p.Gln21Lys",
          "transcript": "ENST00000395080.8",
          "protein_id": "ENSP00000378517.3",
          "transcript_support_level": 1,
          "aa_start": 21,
          "aa_end": null,
          "aa_length": 314,
          "cds_start": 61,
          "cds_end": null,
          "cds_length": 945,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001040058.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000395080.8"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPP1",
          "gene_hgnc_id": 11255,
          "hgvs_c": "c.61C>A",
          "hgvs_p": "p.Gln21Lys",
          "transcript": "ENST00000237623.11",
          "protein_id": "ENSP00000237623.7",
          "transcript_support_level": 1,
          "aa_start": 21,
          "aa_end": null,
          "aa_length": 300,
          "cds_start": 61,
          "cds_end": null,
          "cds_length": 903,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000237623.11"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPP1",
          "gene_hgnc_id": 11255,
          "hgvs_c": "c.61C>A",
          "hgvs_p": "p.Gln21Lys",
          "transcript": "ENST00000360804.4",
          "protein_id": "ENSP00000354042.4",
          "transcript_support_level": 1,
          "aa_start": 21,
          "aa_end": null,
          "aa_length": 287,
          "cds_start": 61,
          "cds_end": null,
          "cds_length": 864,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000360804.4"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPP1",
          "gene_hgnc_id": 11255,
          "hgvs_c": "n.159C>A",
          "hgvs_p": null,
          "transcript": "ENST00000508002.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000508002.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPP1",
          "gene_hgnc_id": 11255,
          "hgvs_c": "n.161C>A",
          "hgvs_p": null,
          "transcript": "ENST00000509659.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000509659.5"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPP1",
          "gene_hgnc_id": 11255,
          "hgvs_c": "c.61C>A",
          "hgvs_p": "p.Gln21Lys",
          "transcript": "ENST00000614857.5",
          "protein_id": "ENSP00000477824.2",
          "transcript_support_level": 2,
          "aa_start": 21,
          "aa_end": null,
          "aa_length": 314,
          "cds_start": 61,
          "cds_end": null,
          "cds_length": 945,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000614857.5"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPP1",
          "gene_hgnc_id": 11255,
          "hgvs_c": "c.61C>A",
          "hgvs_p": "p.Gln21Lys",
          "transcript": "ENST00000882523.1",
          "protein_id": "ENSP00000552582.1",
          "transcript_support_level": null,
          "aa_start": 21,
          "aa_end": null,
          "aa_length": 314,
          "cds_start": 61,
          "cds_end": null,
          "cds_length": 945,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000882523.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPP1",
          "gene_hgnc_id": 11255,
          "hgvs_c": "c.61C>A",
          "hgvs_p": "p.Gln21Lys",
          "transcript": "ENST00000942419.1",
          "protein_id": "ENSP00000612478.1",
          "transcript_support_level": null,
          "aa_start": 21,
          "aa_end": null,
          "aa_length": 314,
          "cds_start": 61,
          "cds_end": null,
          "cds_length": 945,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942419.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPP1",
          "gene_hgnc_id": 11255,
          "hgvs_c": "c.61C>A",
          "hgvs_p": "p.Gln21Lys",
          "transcript": "NM_000582.3",
          "protein_id": "NP_000573.1",
          "transcript_support_level": null,
          "aa_start": 21,
          "aa_end": null,
          "aa_length": 300,
          "cds_start": 61,
          "cds_end": null,
          "cds_length": 903,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000582.3"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPP1",
          "gene_hgnc_id": 11255,
          "hgvs_c": "c.61C>A",
          "hgvs_p": "p.Gln21Lys",
          "transcript": "ENST00000942420.1",
          "protein_id": "ENSP00000612479.1",
          "transcript_support_level": null,
          "aa_start": 21,
          "aa_end": null,
          "aa_length": 300,
          "cds_start": 61,
          "cds_end": null,
          "cds_length": 903,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942420.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPP1",
          "gene_hgnc_id": 11255,
          "hgvs_c": "c.61C>A",
          "hgvs_p": "p.Gln21Lys",
          "transcript": "ENST00000920430.1",
          "protein_id": "ENSP00000590489.1",
          "transcript_support_level": null,
          "aa_start": 21,
          "aa_end": null,
          "aa_length": 295,
          "cds_start": 61,
          "cds_end": null,
          "cds_length": 888,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000920430.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPP1",
          "gene_hgnc_id": 11255,
          "hgvs_c": "c.61C>A",
          "hgvs_p": "p.Gln21Lys",
          "transcript": "NM_001040060.2",
          "protein_id": "NP_001035149.1",
          "transcript_support_level": null,
          "aa_start": 21,
          "aa_end": null,
          "aa_length": 287,
          "cds_start": 61,
          "cds_end": null,
          "cds_length": 864,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001040060.2"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPP1",
          "gene_hgnc_id": 11255,
          "hgvs_c": "c.61C>A",
          "hgvs_p": "p.Gln21Lys",
          "transcript": "ENST00000882524.1",
          "protein_id": "ENSP00000552583.1",
          "transcript_support_level": null,
          "aa_start": 21,
          "aa_end": null,
          "aa_length": 287,
          "cds_start": 61,
          "cds_end": null,
          "cds_length": 864,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000882524.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPP1",
          "gene_hgnc_id": 11255,
          "hgvs_c": "c.61C>A",
          "hgvs_p": "p.Gln21Lys",
          "transcript": "NM_001251829.2",
          "protein_id": "NP_001238758.1",
          "transcript_support_level": null,
          "aa_start": 21,
          "aa_end": null,
          "aa_length": 273,
          "cds_start": 61,
          "cds_end": null,
          "cds_length": 822,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001251829.2"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPP1",
          "gene_hgnc_id": 11255,
          "hgvs_c": "c.61C>A",
          "hgvs_p": "p.Gln21Lys",
          "transcript": "ENST00000508233.6",
          "protein_id": "ENSP00000422973.2",
          "transcript_support_level": 2,
          "aa_start": 21,
          "aa_end": null,
          "aa_length": 273,
          "cds_start": 61,
          "cds_end": null,
          "cds_length": 822,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000508233.6"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPP1",
          "gene_hgnc_id": 11255,
          "hgvs_c": "c.61C>A",
          "hgvs_p": "p.Gln21Lys",
          "transcript": "ENST00000682655.1",
          "protein_id": "ENSP00000508280.1",
          "transcript_support_level": null,
          "aa_start": 21,
          "aa_end": null,
          "aa_length": 192,
          "cds_start": 61,
          "cds_end": null,
          "cds_length": 579,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000682655.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPP1",
          "gene_hgnc_id": 11255,
          "hgvs_c": "c.-90C>A",
          "hgvs_p": null,
          "transcript": "NM_001251830.2",
          "protein_id": "NP_001238759.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 327,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 984,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001251830.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "SPP1",
          "gene_hgnc_id": 11255,
          "hgvs_c": "c.55-3C>A",
          "hgvs_p": null,
          "transcript": "ENST00000882522.1",
          "protein_id": "ENSP00000552581.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 311,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 936,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000882522.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "SPP1",
          "gene_hgnc_id": 11255,
          "hgvs_c": "c.55-3C>A",
          "hgvs_p": null,
          "transcript": "ENST00000882525.1",
          "protein_id": "ENSP00000552584.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 297,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 894,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000882525.1"
        },
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      "gnomad_exomes_af": 0.0000862037,
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      "gnomad_exomes_homalt": 0,
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      "computational_score_selected": 0.059816956520080566,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "revel_prediction": "Benign",
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      "bayesdelnoaf_score": -0.54,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.692,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
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      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong",
      "acmg_by_gene": [
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          "benign_score": 4,
          "pathogenic_score": 2,
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            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001040058.2",
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          "effects": [
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          "inheritance_mode": "Unknown",
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          "hgvs_p": "p.Gln21Lys"
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        {
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            "BP4_Strong"
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          "verdict": "Likely_benign",
          "transcript": "ENST00000662475.1",
          "gene_symbol": "ENSG00000286618",
          "hgnc_id": null,
          "effects": [
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          ],
          "inheritance_mode": "",
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
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  ],
  "message": null
}