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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-989824-C-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=989824&ref=C&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 989824,
      "ref": "C",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_022042.4",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A1",
          "gene_hgnc_id": 10993,
          "hgvs_c": "c.1115G>T",
          "hgvs_p": "p.Arg372Leu",
          "transcript": "NM_022042.4",
          "protein_id": "NP_071325.2",
          "transcript_support_level": null,
          "aa_start": 372,
          "aa_end": null,
          "aa_length": 701,
          "cds_start": 1115,
          "cds_end": null,
          "cds_length": 2106,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000398516.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_022042.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A1",
          "gene_hgnc_id": 10993,
          "hgvs_c": "c.1115G>T",
          "hgvs_p": "p.Arg372Leu",
          "transcript": "ENST00000398516.3",
          "protein_id": "ENSP00000381528.2",
          "transcript_support_level": 1,
          "aa_start": 372,
          "aa_end": null,
          "aa_length": 701,
          "cds_start": 1115,
          "cds_end": null,
          "cds_length": 2106,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_022042.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000398516.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A1",
          "gene_hgnc_id": 10993,
          "hgvs_c": "c.1115G>T",
          "hgvs_p": "p.Arg372Leu",
          "transcript": "ENST00000361661.6",
          "protein_id": "ENSP00000354721.2",
          "transcript_support_level": 1,
          "aa_start": 372,
          "aa_end": null,
          "aa_length": 701,
          "cds_start": 1115,
          "cds_end": null,
          "cds_length": 2106,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000361661.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "IDUA",
          "gene_hgnc_id": 5391,
          "hgvs_c": "c.299+1875C>A",
          "hgvs_p": null,
          "transcript": "NM_000203.5",
          "protein_id": "NP_000194.2",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 653,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1962,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000514224.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000203.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "IDUA",
          "gene_hgnc_id": 5391,
          "hgvs_c": "c.299+1875C>A",
          "hgvs_p": null,
          "transcript": "ENST00000514224.2",
          "protein_id": "ENSP00000425081.2",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 653,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1962,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_000203.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000514224.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "IDUA",
          "gene_hgnc_id": 5391,
          "hgvs_c": "c.299+1875C>A",
          "hgvs_p": null,
          "transcript": "ENST00000247933.9",
          "protein_id": "ENSP00000247933.4",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 653,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1962,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000247933.9"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A1",
          "gene_hgnc_id": 10993,
          "hgvs_c": "c.576+1304G>T",
          "hgvs_p": null,
          "transcript": "ENST00000398520.6",
          "protein_id": "ENSP00000381532.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 224,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 675,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000398520.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A1",
          "gene_hgnc_id": 10993,
          "hgvs_c": "c.1115G>T",
          "hgvs_p": "p.Arg372Leu",
          "transcript": "NM_213613.4",
          "protein_id": "NP_998778.1",
          "transcript_support_level": null,
          "aa_start": 372,
          "aa_end": null,
          "aa_length": 701,
          "cds_start": 1115,
          "cds_end": null,
          "cds_length": 2106,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_213613.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A1",
          "gene_hgnc_id": 10993,
          "hgvs_c": "c.1115G>T",
          "hgvs_p": "p.Arg372Leu",
          "transcript": "ENST00000871988.1",
          "protein_id": "ENSP00000542047.1",
          "transcript_support_level": null,
          "aa_start": 372,
          "aa_end": null,
          "aa_length": 701,
          "cds_start": 1115,
          "cds_end": null,
          "cds_length": 2106,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000871988.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A1",
          "gene_hgnc_id": 10993,
          "hgvs_c": "c.1115G>T",
          "hgvs_p": "p.Arg372Leu",
          "transcript": "ENST00000871989.1",
          "protein_id": "ENSP00000542048.1",
          "transcript_support_level": null,
          "aa_start": 372,
          "aa_end": null,
          "aa_length": 701,
          "cds_start": 1115,
          "cds_end": null,
          "cds_length": 2106,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000871989.1"
        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC26A1",
          "gene_hgnc_id": 10993,
          "hgvs_c": "c.1115G>T",
          "hgvs_p": "p.Arg372Leu",
          "transcript": "ENST00000871990.1",
          "protein_id": "ENSP00000542049.1",
          "transcript_support_level": null,
          "aa_start": 372,
          "aa_end": null,
          "aa_length": 701,
          "cds_start": 1115,
          "cds_end": null,
          "cds_length": 2106,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
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        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
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          "gene_symbol": "SLC26A1",
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          "hgvs_c": "c.1115G>T",
          "hgvs_p": "p.Arg372Leu",
          "transcript": "ENST00000962470.1",
          "protein_id": "ENSP00000632529.1",
          "transcript_support_level": null,
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          "aa_end": null,
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          "cds_start": 1115,
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        {
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          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
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          "exon_count": 15,
          "intron_rank": 2,
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          "gene_symbol": "IDUA",
          "gene_hgnc_id": 5391,
          "hgvs_c": "c.299+1875C>A",
          "hgvs_p": null,
          "transcript": "ENST00000962389.1",
          "protein_id": "ENSP00000632448.1",
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          "cds_start": null,
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        {
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          ],
          "exon_rank": null,
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          "exon_count": 14,
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          "gene_symbol": "IDUA",
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          "hgvs_c": "c.299+1875C>A",
          "hgvs_p": null,
          "transcript": "ENST00000871737.1",
          "protein_id": "ENSP00000541796.1",
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          "hgvs_c": "c.299+1875C>A",
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        {
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          "gene_symbol": "IDUA",
          "gene_hgnc_id": 5391,
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        {
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          "gene_symbol": "IDUA",
          "gene_hgnc_id": 5391,
          "hgvs_c": "c.299+1875C>A",
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          "transcript": "ENST00000871734.1",
          "protein_id": "ENSP00000541793.1",
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          "gene_symbol": "IDUA",
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        },
        {
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          ],
          "exon_rank": null,
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          "exon_count": 13,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "IDUA",
          "gene_hgnc_id": 5391,
          "hgvs_c": "c.299+1875C>A",
          "hgvs_p": null,
          "transcript": "ENST00000871739.1",
          "protein_id": "ENSP00000541798.1",
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          "aa_start": null,
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          "cds_start": null,
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          "biotype": "protein_coding",
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      ],
      "gene_symbol": "SLC26A1",
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      "dbsnp": "rs73219719",
      "frequency_reference_population": 0.00004366381,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 69,
      "gnomad_exomes_af": 0.0000462183,
      "gnomad_genomes_af": 0.0000197044,
      "gnomad_exomes_ac": 66,
      "gnomad_genomes_ac": 3,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.7714924812316895,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.409,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.1263,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.15,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.044,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 3,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3",
      "acmg_by_gene": [
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
            "PM2",
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_022042.4",
          "gene_symbol": "SLC26A1",
          "hgnc_id": 10993,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1115G>T",
          "hgvs_p": "p.Arg372Leu"
        },
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
            "PM2",
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_000203.5",
          "gene_symbol": "IDUA",
          "hgnc_id": 5391,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AR",
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      ],
      "clinvar_disease": "Hyperoxaluria,not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:2",
      "phenotype_combined": "Hyperoxaluria|not provided",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}