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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-112204453-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=112204453&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 112204453,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000261486.6",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPB41L4A",
          "gene_hgnc_id": 13278,
          "hgvs_c": "c.1298C>T",
          "hgvs_p": "p.Ser433Leu",
          "transcript": "NM_022140.5",
          "protein_id": "NP_071423.4",
          "transcript_support_level": null,
          "aa_start": 433,
          "aa_end": null,
          "aa_length": 686,
          "cds_start": 1298,
          "cds_end": null,
          "cds_length": 2061,
          "cdna_start": 1536,
          "cdna_end": null,
          "cdna_length": 4670,
          "mane_select": "ENST00000261486.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPB41L4A",
          "gene_hgnc_id": 13278,
          "hgvs_c": "c.1298C>T",
          "hgvs_p": "p.Ser433Leu",
          "transcript": "ENST00000261486.6",
          "protein_id": "ENSP00000261486.5",
          "transcript_support_level": 1,
          "aa_start": 433,
          "aa_end": null,
          "aa_length": 686,
          "cds_start": 1298,
          "cds_end": null,
          "cds_length": 2061,
          "cdna_start": 1536,
          "cdna_end": null,
          "cdna_length": 4670,
          "mane_select": "NM_022140.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPB41L4A",
          "gene_hgnc_id": 13278,
          "hgvs_c": "c.1298C>T",
          "hgvs_p": "p.Ser433Leu",
          "transcript": "NM_001347887.2",
          "protein_id": "NP_001334816.1",
          "transcript_support_level": null,
          "aa_start": 433,
          "aa_end": null,
          "aa_length": 708,
          "cds_start": 1298,
          "cds_end": null,
          "cds_length": 2127,
          "cdna_start": 1536,
          "cdna_end": null,
          "cdna_length": 3698,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPB41L4A",
          "gene_hgnc_id": 13278,
          "hgvs_c": "c.1298C>T",
          "hgvs_p": "p.Ser433Leu",
          "transcript": "XM_047417471.1",
          "protein_id": "XP_047273427.1",
          "transcript_support_level": null,
          "aa_start": 433,
          "aa_end": null,
          "aa_length": 708,
          "cds_start": 1298,
          "cds_end": null,
          "cds_length": 2127,
          "cdna_start": 1798,
          "cdna_end": null,
          "cdna_length": 3960,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPB41L4A",
          "gene_hgnc_id": 13278,
          "hgvs_c": "c.1298C>T",
          "hgvs_p": "p.Ser433Leu",
          "transcript": "XM_047417472.1",
          "protein_id": "XP_047273428.1",
          "transcript_support_level": null,
          "aa_start": 433,
          "aa_end": null,
          "aa_length": 708,
          "cds_start": 1298,
          "cds_end": null,
          "cds_length": 2127,
          "cdna_start": 1824,
          "cdna_end": null,
          "cdna_length": 3986,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPB41L4A",
          "gene_hgnc_id": 13278,
          "hgvs_c": "c.1298C>T",
          "hgvs_p": "p.Ser433Leu",
          "transcript": "XM_011543531.4",
          "protein_id": "XP_011541833.1",
          "transcript_support_level": null,
          "aa_start": 433,
          "aa_end": null,
          "aa_length": 706,
          "cds_start": 1298,
          "cds_end": null,
          "cds_length": 2121,
          "cdna_start": 1798,
          "cdna_end": null,
          "cdna_length": 3126,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPB41L4A",
          "gene_hgnc_id": 13278,
          "hgvs_c": "c.1298C>T",
          "hgvs_p": "p.Ser433Leu",
          "transcript": "XM_011543532.3",
          "protein_id": "XP_011541834.1",
          "transcript_support_level": null,
          "aa_start": 433,
          "aa_end": null,
          "aa_length": 697,
          "cds_start": 1298,
          "cds_end": null,
          "cds_length": 2094,
          "cdna_start": 1798,
          "cdna_end": null,
          "cdna_length": 3991,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPB41L4A",
          "gene_hgnc_id": 13278,
          "hgvs_c": "c.1298C>T",
          "hgvs_p": "p.Ser433Leu",
          "transcript": "XM_047417474.1",
          "protein_id": "XP_047273430.1",
          "transcript_support_level": null,
          "aa_start": 433,
          "aa_end": null,
          "aa_length": 686,
          "cds_start": 1298,
          "cds_end": null,
          "cds_length": 2061,
          "cdna_start": 1798,
          "cdna_end": null,
          "cdna_length": 4932,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPB41L4A",
          "gene_hgnc_id": 13278,
          "hgvs_c": "c.1298C>T",
          "hgvs_p": "p.Ser433Leu",
          "transcript": "XM_047417475.1",
          "protein_id": "XP_047273431.1",
          "transcript_support_level": null,
          "aa_start": 433,
          "aa_end": null,
          "aa_length": 686,
          "cds_start": 1298,
          "cds_end": null,
          "cds_length": 2061,
          "cdna_start": 1824,
          "cdna_end": null,
          "cdna_length": 4958,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPB41L4A",
          "gene_hgnc_id": 13278,
          "hgvs_c": "c.1214C>T",
          "hgvs_p": "p.Ser405Leu",
          "transcript": "XM_011543533.3",
          "protein_id": "XP_011541835.1",
          "transcript_support_level": null,
          "aa_start": 405,
          "aa_end": null,
          "aa_length": 680,
          "cds_start": 1214,
          "cds_end": null,
          "cds_length": 2043,
          "cdna_start": 1452,
          "cdna_end": null,
          "cdna_length": 3614,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPB41L4A",
          "gene_hgnc_id": 13278,
          "hgvs_c": "c.1214C>T",
          "hgvs_p": "p.Ser405Leu",
          "transcript": "XM_047417476.1",
          "protein_id": "XP_047273432.1",
          "transcript_support_level": null,
          "aa_start": 405,
          "aa_end": null,
          "aa_length": 658,
          "cds_start": 1214,
          "cds_end": null,
          "cds_length": 1977,
          "cdna_start": 1714,
          "cdna_end": null,
          "cdna_length": 4848,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPB41L4A",
          "gene_hgnc_id": 13278,
          "hgvs_c": "c.746C>T",
          "hgvs_p": "p.Ser249Leu",
          "transcript": "XM_047417477.1",
          "protein_id": "XP_047273433.1",
          "transcript_support_level": null,
          "aa_start": 249,
          "aa_end": null,
          "aa_length": 502,
          "cds_start": 746,
          "cds_end": null,
          "cds_length": 1509,
          "cdna_start": 1393,
          "cdna_end": null,
          "cdna_length": 4527,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPB41L4A",
          "gene_hgnc_id": 13278,
          "hgvs_c": "c.746C>T",
          "hgvs_p": "p.Ser249Leu",
          "transcript": "XM_047417478.1",
          "protein_id": "XP_047273434.1",
          "transcript_support_level": null,
          "aa_start": 249,
          "aa_end": null,
          "aa_length": 502,
          "cds_start": 746,
          "cds_end": null,
          "cds_length": 1509,
          "cdna_start": 1459,
          "cdna_end": null,
          "cdna_length": 4593,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000251187",
          "gene_hgnc_id": null,
          "hgvs_c": "n.310G>A",
          "hgvs_p": null,
          "transcript": "ENST00000505825.2",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 688,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000251187",
          "gene_hgnc_id": null,
          "hgvs_c": "n.162G>A",
          "hgvs_p": null,
          "transcript": "ENST00000506875.1",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 494,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPB41L4A",
          "gene_hgnc_id": 13278,
          "hgvs_c": "n.318C>T",
          "hgvs_p": null,
          "transcript": "ENST00000507810.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2552,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPB41L4A",
          "gene_hgnc_id": 13278,
          "hgvs_c": "n.207C>T",
          "hgvs_p": null,
          "transcript": "ENST00000513333.1",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 333,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPB41L4A",
          "gene_hgnc_id": 13278,
          "hgvs_c": "n.118C>T",
          "hgvs_p": null,
          "transcript": "ENST00000515047.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 851,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPB41L4A",
          "gene_hgnc_id": 13278,
          "hgvs_c": "n.1536C>T",
          "hgvs_p": null,
          "transcript": "NR_144931.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4715,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPB41L4A",
          "gene_hgnc_id": 13278,
          "hgvs_c": "n.1536C>T",
          "hgvs_p": null,
          "transcript": "XR_001742173.3",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2895,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000251187",
          "gene_hgnc_id": null,
          "hgvs_c": "n.248-5506G>A",
          "hgvs_p": null,
          "transcript": "ENST00000774363.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 428,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "EPB41L4A",
      "gene_hgnc_id": 13278,
      "dbsnp": "rs730882207",
      "frequency_reference_population": 0.000013014539,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 21,
      "gnomad_exomes_af": 0.0000116324,
      "gnomad_genomes_af": 0.0000262909,
      "gnomad_exomes_ac": 17,
      "gnomad_genomes_ac": 4,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8878631591796875,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.832,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.2363,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.21,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.014,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 5,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3_Moderate,PP5",
      "acmg_by_gene": [
        {
          "score": 5,
          "benign_score": 0,
          "pathogenic_score": 5,
          "criteria": [
            "PM2",
            "PP3_Moderate",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000261486.6",
          "gene_symbol": "EPB41L4A",
          "hgnc_id": 13278,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1298C>T",
          "hgvs_p": "p.Ser433Leu"
        },
        {
          "score": 5,
          "benign_score": 0,
          "pathogenic_score": 5,
          "criteria": [
            "PM2",
            "PP3_Moderate",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000505825.2",
          "gene_symbol": "ENSG00000251187",
          "hgnc_id": null,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.310G>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Failure to thrive,Spastic paraplegia",
      "clinvar_classification": "Likely pathogenic",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "Failure to thrive;Spastic paraplegia",
      "pathogenicity_classification_combined": "Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}