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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-127438533-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=127438533&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 127438533,
      "ref": "C",
      "alt": "T",
      "effect": "synonymous_variant",
      "transcript": "ENST00000503335.7",
      "consequences": [
        {
          "aa_ref": "C",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MEGF10",
          "gene_hgnc_id": 29634,
          "hgvs_c": "c.2199C>T",
          "hgvs_p": "p.Cys733Cys",
          "transcript": "NM_001256545.2",
          "protein_id": "NP_001243474.1",
          "transcript_support_level": null,
          "aa_start": 733,
          "aa_end": null,
          "aa_length": 1140,
          "cds_start": 2199,
          "cds_end": null,
          "cds_length": 3423,
          "cdna_start": 2478,
          "cdna_end": null,
          "cdna_length": 7606,
          "mane_select": "ENST00000503335.7",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MEGF10",
          "gene_hgnc_id": 29634,
          "hgvs_c": "c.2199C>T",
          "hgvs_p": "p.Cys733Cys",
          "transcript": "ENST00000503335.7",
          "protein_id": "ENSP00000423354.2",
          "transcript_support_level": 1,
          "aa_start": 733,
          "aa_end": null,
          "aa_length": 1140,
          "cds_start": 2199,
          "cds_end": null,
          "cds_length": 3423,
          "cdna_start": 2478,
          "cdna_end": null,
          "cdna_length": 7606,
          "mane_select": "NM_001256545.2",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MEGF10",
          "gene_hgnc_id": 29634,
          "hgvs_c": "c.2199C>T",
          "hgvs_p": "p.Cys733Cys",
          "transcript": "ENST00000274473.6",
          "protein_id": "ENSP00000274473.6",
          "transcript_support_level": 1,
          "aa_start": 733,
          "aa_end": null,
          "aa_length": 1140,
          "cds_start": 2199,
          "cds_end": null,
          "cds_length": 3423,
          "cdna_start": 2466,
          "cdna_end": null,
          "cdna_length": 7594,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MEGF10",
          "gene_hgnc_id": 29634,
          "hgvs_c": "c.2199C>T",
          "hgvs_p": "p.Cys733Cys",
          "transcript": "NM_032446.3",
          "protein_id": "NP_115822.1",
          "transcript_support_level": null,
          "aa_start": 733,
          "aa_end": null,
          "aa_length": 1140,
          "cds_start": 2199,
          "cds_end": null,
          "cds_length": 3423,
          "cdna_start": 2529,
          "cdna_end": null,
          "cdna_length": 7657,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MEGF10",
          "gene_hgnc_id": 29634,
          "hgvs_c": "c.2364C>T",
          "hgvs_p": "p.Cys788Cys",
          "transcript": "XM_017009987.2",
          "protein_id": "XP_016865476.1",
          "transcript_support_level": null,
          "aa_start": 788,
          "aa_end": null,
          "aa_length": 1195,
          "cds_start": 2364,
          "cds_end": null,
          "cds_length": 3588,
          "cdna_start": 3025,
          "cdna_end": null,
          "cdna_length": 8153,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MEGF10",
          "gene_hgnc_id": 29634,
          "hgvs_c": "c.2199C>T",
          "hgvs_p": "p.Cys733Cys",
          "transcript": "XM_011543694.1",
          "protein_id": "XP_011541996.1",
          "transcript_support_level": null,
          "aa_start": 733,
          "aa_end": null,
          "aa_length": 1140,
          "cds_start": 2199,
          "cds_end": null,
          "cds_length": 3423,
          "cdna_start": 2273,
          "cdna_end": null,
          "cdna_length": 7401,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MEGF10",
          "gene_hgnc_id": 29634,
          "hgvs_c": "c.1059C>T",
          "hgvs_p": "p.Cys353Cys",
          "transcript": "XM_017009988.2",
          "protein_id": "XP_016865477.1",
          "transcript_support_level": null,
          "aa_start": 353,
          "aa_end": null,
          "aa_length": 760,
          "cds_start": 1059,
          "cds_end": null,
          "cds_length": 2283,
          "cdna_start": 5460,
          "cdna_end": null,
          "cdna_length": 10588,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MEGF10",
          "gene_hgnc_id": 29634,
          "hgvs_c": "n.440C>T",
          "hgvs_p": null,
          "transcript": "ENST00000506709.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 583,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "MEGF10",
      "gene_hgnc_id": 29634,
      "dbsnp": "rs35591368",
      "frequency_reference_population": 0.030756202,
      "hom_count_reference_population": 854,
      "allele_count_reference_population": 49641,
      "gnomad_exomes_af": 0.0312494,
      "gnomad_genomes_af": 0.026022,
      "gnomad_exomes_ac": 45678,
      "gnomad_genomes_ac": 3963,
      "gnomad_exomes_homalt": 805,
      "gnomad_genomes_homalt": 49,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": -0.4300000071525574,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0.029999999329447746,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.43,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.287,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.03,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -19,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Moderate,BP6_Very_Strong,BP7,BS1,BS2",
      "acmg_by_gene": [
        {
          "score": -19,
          "benign_score": 19,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BP6_Very_Strong",
            "BP7",
            "BS1",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000503335.7",
          "gene_symbol": "MEGF10",
          "hgnc_id": 29634,
          "effects": [
            "synonymous_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2199C>T",
          "hgvs_p": "p.Cys733Cys"
        }
      ],
      "clinvar_disease": "MEGF10-related myopathy,not provided,not specified",
      "clinvar_classification": "Benign/Likely benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LB:2 B:3",
      "phenotype_combined": "not specified|MEGF10-related myopathy|not provided",
      "pathogenicity_classification_combined": "Benign/Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}