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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-132213966-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=132213966&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 132213966,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "ENST00000360568.8",
      "consequences": [
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HA2",
          "gene_hgnc_id": 8547,
          "hgvs_c": "c.419A>G",
          "hgvs_p": "p.Gln140Arg",
          "transcript": "NM_001365677.2",
          "protein_id": "NP_001352606.1",
          "transcript_support_level": null,
          "aa_start": 140,
          "aa_end": null,
          "aa_length": 535,
          "cds_start": 419,
          "cds_end": null,
          "cds_length": 1608,
          "cdna_start": 501,
          "cdna_end": null,
          "cdna_length": 4553,
          "mane_select": null,
          "mane_plus": "ENST00000379104.7",
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HA2",
          "gene_hgnc_id": 8547,
          "hgvs_c": "c.419A>G",
          "hgvs_p": "p.Gln140Arg",
          "transcript": "ENST00000379104.7",
          "protein_id": "ENSP00000368398.2",
          "transcript_support_level": 1,
          "aa_start": 140,
          "aa_end": null,
          "aa_length": 535,
          "cds_start": 419,
          "cds_end": null,
          "cds_length": 1608,
          "cdna_start": 501,
          "cdna_end": null,
          "cdna_length": 4553,
          "mane_select": null,
          "mane_plus": "NM_001365677.2",
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HA2",
          "gene_hgnc_id": 8547,
          "hgvs_c": "c.419A>G",
          "hgvs_p": "p.Gln140Arg",
          "transcript": "NM_001017974.2",
          "protein_id": "NP_001017974.1",
          "transcript_support_level": null,
          "aa_start": 140,
          "aa_end": null,
          "aa_length": 533,
          "cds_start": 419,
          "cds_end": null,
          "cds_length": 1602,
          "cdna_start": 501,
          "cdna_end": null,
          "cdna_length": 4547,
          "mane_select": "ENST00000360568.8",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HA2",
          "gene_hgnc_id": 8547,
          "hgvs_c": "c.419A>G",
          "hgvs_p": "p.Gln140Arg",
          "transcript": "ENST00000360568.8",
          "protein_id": "ENSP00000353772.3",
          "transcript_support_level": 1,
          "aa_start": 140,
          "aa_end": null,
          "aa_length": 533,
          "cds_start": 419,
          "cds_end": null,
          "cds_length": 1602,
          "cdna_start": 501,
          "cdna_end": null,
          "cdna_length": 4547,
          "mane_select": "NM_001017974.2",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HA2",
          "gene_hgnc_id": 8547,
          "hgvs_c": "c.419A>G",
          "hgvs_p": "p.Gln140Arg",
          "transcript": "ENST00000166534.8",
          "protein_id": "ENSP00000166534.4",
          "transcript_support_level": 1,
          "aa_start": 140,
          "aa_end": null,
          "aa_length": 535,
          "cds_start": 419,
          "cds_end": null,
          "cds_length": 1608,
          "cdna_start": 641,
          "cdna_end": null,
          "cdna_length": 2230,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HA2",
          "gene_hgnc_id": 8547,
          "hgvs_c": "c.419A>G",
          "hgvs_p": "p.Gln140Arg",
          "transcript": "NM_001142599.2",
          "protein_id": "NP_001136071.1",
          "transcript_support_level": null,
          "aa_start": 140,
          "aa_end": null,
          "aa_length": 535,
          "cds_start": 419,
          "cds_end": null,
          "cds_length": 1608,
          "cdna_start": 637,
          "cdna_end": null,
          "cdna_length": 4689,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HA2",
          "gene_hgnc_id": 8547,
          "hgvs_c": "c.419A>G",
          "hgvs_p": "p.Gln140Arg",
          "transcript": "NM_004199.3",
          "protein_id": "NP_004190.1",
          "transcript_support_level": null,
          "aa_start": 140,
          "aa_end": null,
          "aa_length": 535,
          "cds_start": 419,
          "cds_end": null,
          "cds_length": 1608,
          "cdna_start": 832,
          "cdna_end": null,
          "cdna_length": 4884,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HA2",
          "gene_hgnc_id": 8547,
          "hgvs_c": "c.419A>G",
          "hgvs_p": "p.Gln140Arg",
          "transcript": "ENST00000401867.5",
          "protein_id": "ENSP00000384999.1",
          "transcript_support_level": 5,
          "aa_start": 140,
          "aa_end": null,
          "aa_length": 535,
          "cds_start": 419,
          "cds_end": null,
          "cds_length": 1608,
          "cdna_start": 988,
          "cdna_end": null,
          "cdna_length": 3349,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HA2",
          "gene_hgnc_id": 8547,
          "hgvs_c": "c.419A>G",
          "hgvs_p": "p.Gln140Arg",
          "transcript": "NM_001142598.2",
          "protein_id": "NP_001136070.1",
          "transcript_support_level": null,
          "aa_start": 140,
          "aa_end": null,
          "aa_length": 533,
          "cds_start": 419,
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          "cds_length": 1602,
          "cdna_start": 637,
          "cdna_end": null,
          "cdna_length": 4683,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HA2",
          "gene_hgnc_id": 8547,
          "hgvs_c": "c.419A>G",
          "hgvs_p": "p.Gln140Arg",
          "transcript": "NM_001365678.2",
          "protein_id": "NP_001352607.1",
          "transcript_support_level": null,
          "aa_start": 140,
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          "cds_start": 419,
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          "cdna_start": 674,
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          "mane_select": null,
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        {
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          "gene_symbol": "P4HA2",
          "gene_hgnc_id": 8547,
          "hgvs_c": "c.419A>G",
          "hgvs_p": "p.Gln140Arg",
          "transcript": "NM_001365679.2",
          "protein_id": "NP_001352608.1",
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          "aa_length": 533,
          "cds_start": 419,
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          "cdna_start": 1063,
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        {
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          "consequences": [
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          "intron_rank": null,
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          "gene_symbol": "P4HA2",
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          "hgvs_c": "c.419A>G",
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        {
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          "gene_symbol": "P4HA2",
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          "hgvs_c": "c.419A>G",
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          "transcript": "ENST00000379100.7",
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        {
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        {
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          "gene_symbol": "P4HA2",
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          "hgvs_c": "c.515A>G",
          "hgvs_p": "p.Gln172Arg",
          "transcript": "ENST00000431054.5",
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        {
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        {
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        {
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        {
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          "gene_symbol": "P4HA2",
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          "hgvs_c": "c.419A>G",
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        },
        {
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          ],
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          "gene_symbol": "P4HA2",
          "gene_hgnc_id": 8547,
          "hgvs_c": "c.419A>G",
          "hgvs_p": "p.Gln140Arg",
          "transcript": "ENST00000428369.6",
          "protein_id": "ENSP00000396495.1",
          "transcript_support_level": 2,
          "aa_start": 140,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 419,
          "cds_end": null,
          "cds_length": 440,
          "cdna_start": 913,
          "cdna_end": null,
          "cdna_length": 2397,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "P4HA2",
          "gene_hgnc_id": 8547,
          "hgvs_c": "c.*75A>G",
          "hgvs_p": null,
          "transcript": "ENST00000418055.5",
          "protein_id": "ENSP00000403883.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 113,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 344,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 568,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "P4HA2",
      "gene_hgnc_id": 8547,
      "dbsnp": "rs764211125",
      "frequency_reference_population": 0.000011151933,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 18,
      "gnomad_exomes_af": 0.00000957713,
      "gnomad_genomes_af": 0.0000262719,
      "gnomad_exomes_ac": 14,
      "gnomad_genomes_ac": 4,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9169716238975525,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.934,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9042,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.25,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 6.239,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -1,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PP3_Moderate,PP5,BS2",
      "acmg_by_gene": [
        {
          "score": -1,
          "benign_score": 4,
          "pathogenic_score": 3,
          "criteria": [
            "PP3_Moderate",
            "PP5",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000360568.8",
          "gene_symbol": "P4HA2",
          "hgnc_id": 8547,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "Unknown,AD",
          "hgvs_c": "c.419A>G",
          "hgvs_p": "p.Gln140Arg"
        }
      ],
      "clinvar_disease": " autosomal dominant,Myopia 25",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "Myopia 25, autosomal dominant",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}