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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-132823005-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=132823005&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 132823005,
      "ref": "C",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_001172700.2",
      "consequences": [
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHROOM1",
          "gene_hgnc_id": 24084,
          "hgvs_c": "c.2350G>C",
          "hgvs_p": "p.Glu784Gln",
          "transcript": "NM_001172700.2",
          "protein_id": "NP_001166171.1",
          "transcript_support_level": null,
          "aa_start": 784,
          "aa_end": null,
          "aa_length": 852,
          "cds_start": 2350,
          "cds_end": null,
          "cds_length": 2559,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000378679.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001172700.2"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHROOM1",
          "gene_hgnc_id": 24084,
          "hgvs_c": "c.2350G>C",
          "hgvs_p": "p.Glu784Gln",
          "transcript": "ENST00000378679.8",
          "protein_id": "ENSP00000367950.3",
          "transcript_support_level": 1,
          "aa_start": 784,
          "aa_end": null,
          "aa_length": 852,
          "cds_start": 2350,
          "cds_end": null,
          "cds_length": 2559,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001172700.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000378679.8"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHROOM1",
          "gene_hgnc_id": 24084,
          "hgvs_c": "c.2335G>C",
          "hgvs_p": "p.Glu779Gln",
          "transcript": "ENST00000319854.7",
          "protein_id": "ENSP00000324245.3",
          "transcript_support_level": 1,
          "aa_start": 779,
          "aa_end": null,
          "aa_length": 847,
          "cds_start": 2335,
          "cds_end": null,
          "cds_length": 2544,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000319854.7"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHROOM1",
          "gene_hgnc_id": 24084,
          "hgvs_c": "c.2350G>C",
          "hgvs_p": "p.Glu784Gln",
          "transcript": "ENST00000617339.4",
          "protein_id": "ENSP00000478436.1",
          "transcript_support_level": 5,
          "aa_start": 784,
          "aa_end": null,
          "aa_length": 852,
          "cds_start": 2350,
          "cds_end": null,
          "cds_length": 2559,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000617339.4"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHROOM1",
          "gene_hgnc_id": 24084,
          "hgvs_c": "c.2350G>C",
          "hgvs_p": "p.Glu784Gln",
          "transcript": "ENST00000891704.1",
          "protein_id": "ENSP00000561763.1",
          "transcript_support_level": null,
          "aa_start": 784,
          "aa_end": null,
          "aa_length": 852,
          "cds_start": 2350,
          "cds_end": null,
          "cds_length": 2559,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000891704.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHROOM1",
          "gene_hgnc_id": 24084,
          "hgvs_c": "c.2350G>C",
          "hgvs_p": "p.Glu784Gln",
          "transcript": "ENST00000891705.1",
          "protein_id": "ENSP00000561764.1",
          "transcript_support_level": null,
          "aa_start": 784,
          "aa_end": null,
          "aa_length": 852,
          "cds_start": 2350,
          "cds_end": null,
          "cds_length": 2559,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000891705.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHROOM1",
          "gene_hgnc_id": 24084,
          "hgvs_c": "c.2350G>C",
          "hgvs_p": "p.Glu784Gln",
          "transcript": "ENST00000891706.1",
          "protein_id": "ENSP00000561765.1",
          "transcript_support_level": null,
          "aa_start": 784,
          "aa_end": null,
          "aa_length": 852,
          "cds_start": 2350,
          "cds_end": null,
          "cds_length": 2559,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000891706.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHROOM1",
          "gene_hgnc_id": 24084,
          "hgvs_c": "c.2350G>C",
          "hgvs_p": "p.Glu784Gln",
          "transcript": "ENST00000891707.1",
          "protein_id": "ENSP00000561766.1",
          "transcript_support_level": null,
          "aa_start": 784,
          "aa_end": null,
          "aa_length": 852,
          "cds_start": 2350,
          "cds_end": null,
          "cds_length": 2559,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000891707.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHROOM1",
          "gene_hgnc_id": 24084,
          "hgvs_c": "c.2350G>C",
          "hgvs_p": "p.Glu784Gln",
          "transcript": "ENST00000891708.1",
          "protein_id": "ENSP00000561767.1",
          "transcript_support_level": null,
          "aa_start": 784,
          "aa_end": null,
          "aa_length": 852,
          "cds_start": 2350,
          "cds_end": null,
          "cds_length": 2559,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000891708.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHROOM1",
          "gene_hgnc_id": 24084,
          "hgvs_c": "c.2350G>C",
          "hgvs_p": "p.Glu784Gln",
          "transcript": "ENST00000891710.1",
          "protein_id": "ENSP00000561769.1",
          "transcript_support_level": null,
          "aa_start": 784,
          "aa_end": null,
          "aa_length": 852,
          "cds_start": 2350,
          "cds_end": null,
          "cds_length": 2559,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000891710.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHROOM1",
          "gene_hgnc_id": 24084,
          "hgvs_c": "c.2350G>C",
          "hgvs_p": "p.Glu784Gln",
          "transcript": "ENST00000891711.1",
          "protein_id": "ENSP00000561770.1",
          "transcript_support_level": null,
          "aa_start": 784,
          "aa_end": null,
          "aa_length": 852,
          "cds_start": 2350,
          "cds_end": null,
          "cds_length": 2559,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000891711.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHROOM1",
          "gene_hgnc_id": 24084,
          "hgvs_c": "c.2350G>C",
          "hgvs_p": "p.Glu784Gln",
          "transcript": "ENST00000891712.1",
          "protein_id": "ENSP00000561771.1",
          "transcript_support_level": null,
          "aa_start": 784,
          "aa_end": null,
          "aa_length": 852,
          "cds_start": 2350,
          "cds_end": null,
          "cds_length": 2559,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000891712.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHROOM1",
          "gene_hgnc_id": 24084,
          "hgvs_c": "c.2350G>C",
          "hgvs_p": "p.Glu784Gln",
          "transcript": "ENST00000891713.1",
          "protein_id": "ENSP00000561772.1",
          "transcript_support_level": null,
          "aa_start": 784,
          "aa_end": null,
          "aa_length": 852,
          "cds_start": 2350,
          "cds_end": null,
          "cds_length": 2559,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000891713.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHROOM1",
          "gene_hgnc_id": 24084,
          "hgvs_c": "c.2350G>C",
          "hgvs_p": "p.Glu784Gln",
          "transcript": "ENST00000891714.1",
          "protein_id": "ENSP00000561773.1",
          "transcript_support_level": null,
          "aa_start": 784,
          "aa_end": null,
          "aa_length": 852,
          "cds_start": 2350,
          "cds_end": null,
          "cds_length": 2559,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000891714.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHROOM1",
          "gene_hgnc_id": 24084,
          "hgvs_c": "c.2350G>C",
          "hgvs_p": "p.Glu784Gln",
          "transcript": "ENST00000891715.1",
          "protein_id": "ENSP00000561774.1",
          "transcript_support_level": null,
          "aa_start": 784,
          "aa_end": null,
          "aa_length": 852,
          "cds_start": 2350,
          "cds_end": null,
          "cds_length": 2559,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000891715.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHROOM1",
          "gene_hgnc_id": 24084,
          "hgvs_c": "c.2350G>C",
          "hgvs_p": "p.Glu784Gln",
          "transcript": "ENST00000891716.1",
          "protein_id": "ENSP00000561775.1",
          "transcript_support_level": null,
          "aa_start": 784,
          "aa_end": null,
          "aa_length": 852,
          "cds_start": 2350,
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          "cds_length": 2559,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000891716.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHROOM1",
          "gene_hgnc_id": 24084,
          "hgvs_c": "c.2350G>C",
          "hgvs_p": "p.Glu784Gln",
          "transcript": "ENST00000891717.1",
          "protein_id": "ENSP00000561776.1",
          "transcript_support_level": null,
          "aa_start": 784,
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          "aa_length": 852,
          "cds_start": 2350,
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          "cdna_start": null,
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          "cdna_length": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000891717.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHROOM1",
          "gene_hgnc_id": 24084,
          "hgvs_c": "c.2350G>C",
          "hgvs_p": "p.Glu784Gln",
          "transcript": "ENST00000924501.1",
          "protein_id": "ENSP00000594560.1",
          "transcript_support_level": null,
          "aa_start": 784,
          "aa_end": null,
          "aa_length": 852,
          "cds_start": 2350,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000924501.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHROOM1",
          "gene_hgnc_id": 24084,
          "hgvs_c": "c.2350G>C",
          "hgvs_p": "p.Glu784Gln",
          "transcript": "ENST00000924502.1",
          "protein_id": "ENSP00000594561.1",
          "transcript_support_level": null,
          "aa_start": 784,
          "aa_end": null,
          "aa_length": 852,
          "cds_start": 2350,
          "cds_end": null,
          "cds_length": 2559,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000924502.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHROOM1",
          "gene_hgnc_id": 24084,
          "hgvs_c": "c.2350G>C",
          "hgvs_p": "p.Glu784Gln",
          "transcript": "ENST00000949960.1",
          "protein_id": "ENSP00000620019.1",
          "transcript_support_level": null,
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          "hgvs_p": "p.Glu784Gln",
          "transcript": "XM_005271886.4",
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        {
          "aa_ref": "E",
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          "protein_coding": true,
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          "cdna_start": null,
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        {
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          "hgvs_c": "c.2350G>C",
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          "biotype": "protein_coding",
          "feature": "XM_047416731.1"
        },
        {
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          "protein_coding": true,
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          ],
          "exon_rank": 9,
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          "exon_count": 9,
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          "gene_symbol": "SHROOM1",
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          "hgvs_c": "c.2350G>C",
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          "biotype": "protein_coding",
          "feature": "XM_047416732.1"
        },
        {
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          "protein_coding": true,
          "strand": false,
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          ],
          "exon_rank": 8,
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          "exon_count": 8,
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          "gene_symbol": "SHROOM1",
          "gene_hgnc_id": 24084,
          "hgvs_c": "c.2143G>C",
          "hgvs_p": "p.Glu715Gln",
          "transcript": "XM_047416733.1",
          "protein_id": "XP_047272689.1",
          "transcript_support_level": null,
          "aa_start": 715,
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          "cds_start": 2143,
          "cds_end": null,
          "cds_length": 2352,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_047416733.1"
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      ],
      "gene_symbol": "SHROOM1",
      "gene_hgnc_id": 24084,
      "dbsnp": "rs953659192",
      "frequency_reference_population": 0.0000012460764,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 2,
      "gnomad_exomes_af": 6.88321e-7,
      "gnomad_genomes_af": 0.00000656909,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.24002715945243835,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.108,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1108,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.53,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.355,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001172700.2",
          "gene_symbol": "SHROOM1",
          "hgnc_id": 24084,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2350G>C",
          "hgvs_p": "p.Glu784Gln"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}