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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-133070378-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=133070378&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 133070378,
      "ref": "C",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_002154.4",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HSPA4",
          "gene_hgnc_id": 5237,
          "hgvs_c": "c.311C>G",
          "hgvs_p": "p.Thr104Arg",
          "transcript": "NM_002154.4",
          "protein_id": "NP_002145.3",
          "transcript_support_level": null,
          "aa_start": 104,
          "aa_end": null,
          "aa_length": 840,
          "cds_start": 311,
          "cds_end": null,
          "cds_length": 2523,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000304858.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_002154.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HSPA4",
          "gene_hgnc_id": 5237,
          "hgvs_c": "c.311C>G",
          "hgvs_p": "p.Thr104Arg",
          "transcript": "ENST00000304858.7",
          "protein_id": "ENSP00000302961.2",
          "transcript_support_level": 1,
          "aa_start": 104,
          "aa_end": null,
          "aa_length": 840,
          "cds_start": 311,
          "cds_end": null,
          "cds_length": 2523,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_002154.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000304858.7"
        },
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HSPA4",
          "gene_hgnc_id": 5237,
          "hgvs_c": "c.311C>G",
          "hgvs_p": "p.Thr104Arg",
          "transcript": "ENST00000968145.1",
          "protein_id": "ENSP00000638204.1",
          "transcript_support_level": null,
          "aa_start": 104,
          "aa_end": null,
          "aa_length": 870,
          "cds_start": 311,
          "cds_end": null,
          "cds_length": 2613,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968145.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HSPA4",
          "gene_hgnc_id": 5237,
          "hgvs_c": "c.311C>G",
          "hgvs_p": "p.Thr104Arg",
          "transcript": "ENST00000936301.1",
          "protein_id": "ENSP00000606360.1",
          "transcript_support_level": null,
          "aa_start": 104,
          "aa_end": null,
          "aa_length": 847,
          "cds_start": 311,
          "cds_end": null,
          "cds_length": 2544,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000936301.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HSPA4",
          "gene_hgnc_id": 5237,
          "hgvs_c": "c.311C>G",
          "hgvs_p": "p.Thr104Arg",
          "transcript": "ENST00000870715.1",
          "protein_id": "ENSP00000540774.1",
          "transcript_support_level": null,
          "aa_start": 104,
          "aa_end": null,
          "aa_length": 839,
          "cds_start": 311,
          "cds_end": null,
          "cds_length": 2520,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000870715.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HSPA4",
          "gene_hgnc_id": 5237,
          "hgvs_c": "c.311C>G",
          "hgvs_p": "p.Thr104Arg",
          "transcript": "ENST00000936299.1",
          "protein_id": "ENSP00000606358.1",
          "transcript_support_level": null,
          "aa_start": 104,
          "aa_end": null,
          "aa_length": 839,
          "cds_start": 311,
          "cds_end": null,
          "cds_length": 2520,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000936299.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HSPA4",
          "gene_hgnc_id": 5237,
          "hgvs_c": "c.311C>G",
          "hgvs_p": "p.Thr104Arg",
          "transcript": "ENST00000870717.1",
          "protein_id": "ENSP00000540776.1",
          "transcript_support_level": null,
          "aa_start": 104,
          "aa_end": null,
          "aa_length": 837,
          "cds_start": 311,
          "cds_end": null,
          "cds_length": 2514,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000870717.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HSPA4",
          "gene_hgnc_id": 5237,
          "hgvs_c": "c.311C>G",
          "hgvs_p": "p.Thr104Arg",
          "transcript": "ENST00000968147.1",
          "protein_id": "ENSP00000638206.1",
          "transcript_support_level": null,
          "aa_start": 104,
          "aa_end": null,
          "aa_length": 836,
          "cds_start": 311,
          "cds_end": null,
          "cds_length": 2511,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968147.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HSPA4",
          "gene_hgnc_id": 5237,
          "hgvs_c": "c.311C>G",
          "hgvs_p": "p.Thr104Arg",
          "transcript": "ENST00000968144.1",
          "protein_id": "ENSP00000638203.1",
          "transcript_support_level": null,
          "aa_start": 104,
          "aa_end": null,
          "aa_length": 835,
          "cds_start": 311,
          "cds_end": null,
          "cds_length": 2508,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968144.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HSPA4",
          "gene_hgnc_id": 5237,
          "hgvs_c": "c.311C>G",
          "hgvs_p": "p.Thr104Arg",
          "transcript": "ENST00000870716.1",
          "protein_id": "ENSP00000540775.1",
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          "cds_start": 311,
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        },
        {
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          "consequences": [
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          ],
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          "exon_count": 19,
          "intron_rank": null,
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          "gene_symbol": "HSPA4",
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          "hgvs_c": "c.311C>G",
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          "transcript": "ENST00000936302.1",
          "protein_id": "ENSP00000606361.1",
          "transcript_support_level": null,
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          "cds_start": 311,
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          "cdna_start": null,
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        {
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          "transcript": "ENST00000870721.1",
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        {
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          ],
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          "hgvs_c": "c.311C>G",
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        {
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          ],
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          "transcript": "ENST00000936305.1",
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        {
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        {
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          "intron_rank": null,
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          "gene_hgnc_id": 5237,
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          "transcript": "ENST00000870719.1",
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        {
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          "gene_symbol": "HSPA4",
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        {
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          ],
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          "exon_count": 13,
          "intron_rank": 1,
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          "gene_symbol": "HSPA4",
          "gene_hgnc_id": 5237,
          "hgvs_c": "c.108-16404C>G",
          "hgvs_p": null,
          "transcript": "ENST00000936303.1",
          "protein_id": "ENSP00000606362.1",
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          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000936303.1"
        }
      ],
      "gene_symbol": "HSPA4",
      "gene_hgnc_id": 5237,
      "dbsnp": "rs1459462893",
      "frequency_reference_population": 0.000014290383,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 23,
      "gnomad_exomes_af": 0.0000144055,
      "gnomad_genomes_af": 0.0000131841,
      "gnomad_exomes_ac": 21,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.11389553546905518,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.061,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0956,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.68,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.026,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 2,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_002154.4",
          "gene_symbol": "HSPA4",
          "hgnc_id": 5237,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.311C>G",
          "hgvs_p": "p.Thr104Arg"
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}