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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-133992969-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=133992969&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 133992969,
      "ref": "C",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_001401028.1",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VDAC1",
          "gene_hgnc_id": 12669,
          "hgvs_c": "c.44G>C",
          "hgvs_p": "p.Arg15Thr",
          "transcript": "NM_003374.3",
          "protein_id": "NP_003365.1",
          "transcript_support_level": null,
          "aa_start": 15,
          "aa_end": null,
          "aa_length": 283,
          "cds_start": 44,
          "cds_end": null,
          "cds_length": 852,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000265333.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_003374.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VDAC1",
          "gene_hgnc_id": 12669,
          "hgvs_c": "c.44G>C",
          "hgvs_p": "p.Arg15Thr",
          "transcript": "ENST00000265333.8",
          "protein_id": "ENSP00000265333.3",
          "transcript_support_level": 2,
          "aa_start": 15,
          "aa_end": null,
          "aa_length": 283,
          "cds_start": 44,
          "cds_end": null,
          "cds_length": 852,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_003374.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000265333.8"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VDAC1",
          "gene_hgnc_id": 12669,
          "hgvs_c": "c.44G>C",
          "hgvs_p": "p.Arg15Thr",
          "transcript": "ENST00000395044.7",
          "protein_id": "ENSP00000378484.3",
          "transcript_support_level": 1,
          "aa_start": 15,
          "aa_end": null,
          "aa_length": 283,
          "cds_start": 44,
          "cds_end": null,
          "cds_length": 852,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000395044.7"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VDAC1",
          "gene_hgnc_id": 12669,
          "hgvs_c": "c.44G>C",
          "hgvs_p": "p.Arg15Thr",
          "transcript": "ENST00000395047.6",
          "protein_id": "ENSP00000378487.2",
          "transcript_support_level": 1,
          "aa_start": 15,
          "aa_end": null,
          "aa_length": 283,
          "cds_start": 44,
          "cds_end": null,
          "cds_length": 852,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000395047.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VDAC1",
          "gene_hgnc_id": 12669,
          "hgvs_c": "c.44G>C",
          "hgvs_p": "p.Arg15Thr",
          "transcript": "NM_001401028.1",
          "protein_id": "NP_001387957.1",
          "transcript_support_level": null,
          "aa_start": 15,
          "aa_end": null,
          "aa_length": 297,
          "cds_start": 44,
          "cds_end": null,
          "cds_length": 894,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001401028.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VDAC1",
          "gene_hgnc_id": 12669,
          "hgvs_c": "c.44G>C",
          "hgvs_p": "p.Arg15Thr",
          "transcript": "ENST00000876054.1",
          "protein_id": "ENSP00000546113.1",
          "transcript_support_level": null,
          "aa_start": 15,
          "aa_end": null,
          "aa_length": 297,
          "cds_start": 44,
          "cds_end": null,
          "cds_length": 894,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000876054.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VDAC1",
          "gene_hgnc_id": 12669,
          "hgvs_c": "c.44G>C",
          "hgvs_p": "p.Arg15Thr",
          "transcript": "ENST00000876057.1",
          "protein_id": "ENSP00000546116.1",
          "transcript_support_level": null,
          "aa_start": 15,
          "aa_end": null,
          "aa_length": 297,
          "cds_start": 44,
          "cds_end": null,
          "cds_length": 894,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000876057.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VDAC1",
          "gene_hgnc_id": 12669,
          "hgvs_c": "c.44G>C",
          "hgvs_p": "p.Arg15Thr",
          "transcript": "ENST00000935310.1",
          "protein_id": "ENSP00000605369.1",
          "transcript_support_level": null,
          "aa_start": 15,
          "aa_end": null,
          "aa_length": 297,
          "cds_start": 44,
          "cds_end": null,
          "cds_length": 894,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935310.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VDAC1",
          "gene_hgnc_id": 12669,
          "hgvs_c": "c.44G>C",
          "hgvs_p": "p.Arg15Thr",
          "transcript": "ENST00000876058.1",
          "protein_id": "ENSP00000546117.1",
          "transcript_support_level": null,
          "aa_start": 15,
          "aa_end": null,
          "aa_length": 296,
          "cds_start": 44,
          "cds_end": null,
          "cds_length": 891,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000876058.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VDAC1",
          "gene_hgnc_id": 12669,
          "hgvs_c": "c.44G>C",
          "hgvs_p": "p.Arg15Thr",
          "transcript": "ENST00000935312.1",
          "protein_id": "ENSP00000605371.1",
          "transcript_support_level": null,
          "aa_start": 15,
          "aa_end": null,
          "aa_length": 296,
          "cds_start": 44,
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          "cds_length": 891,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VDAC1",
          "gene_hgnc_id": 12669,
          "hgvs_c": "c.44G>C",
          "hgvs_p": "p.Arg15Thr",
          "transcript": "ENST00000961138.1",
          "protein_id": "ENSP00000631197.1",
          "transcript_support_level": null,
          "aa_start": 15,
          "aa_end": null,
          "aa_length": 295,
          "cds_start": 44,
          "cds_end": null,
          "cds_length": 888,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000961138.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
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          "gene_symbol": "VDAC1",
          "gene_hgnc_id": 12669,
          "hgvs_c": "c.44G>C",
          "hgvs_p": "p.Arg15Thr",
          "transcript": "ENST00000876059.1",
          "protein_id": "ENSP00000546118.1",
          "transcript_support_level": null,
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          "aa_length": 292,
          "cds_start": 44,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VDAC1",
          "gene_hgnc_id": 12669,
          "hgvs_c": "c.44G>C",
          "hgvs_p": "p.Arg15Thr",
          "transcript": "ENST00000876065.1",
          "protein_id": "ENSP00000546124.1",
          "transcript_support_level": null,
          "aa_start": 15,
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          "cds_start": 44,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VDAC1",
          "gene_hgnc_id": 12669,
          "hgvs_c": "c.44G>C",
          "hgvs_p": "p.Arg15Thr",
          "transcript": "ENST00000876056.1",
          "protein_id": "ENSP00000546115.1",
          "transcript_support_level": null,
          "aa_start": 15,
          "aa_end": null,
          "aa_length": 291,
          "cds_start": 44,
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          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000876056.1"
        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": false,
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          ],
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          "intron_rank": null,
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          "gene_symbol": "VDAC1",
          "gene_hgnc_id": 12669,
          "hgvs_c": "c.44G>C",
          "hgvs_p": "p.Arg15Thr",
          "transcript": "NM_001401008.1",
          "protein_id": "NP_001387937.1",
          "transcript_support_level": null,
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          "cds_start": 44,
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          "cds_length": 852,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001401008.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VDAC1",
          "gene_hgnc_id": 12669,
          "hgvs_c": "c.44G>C",
          "hgvs_p": "p.Arg15Thr",
          "transcript": "NM_001401009.1",
          "protein_id": "NP_001387938.1",
          "transcript_support_level": null,
          "aa_start": 15,
          "aa_end": null,
          "aa_length": 283,
          "cds_start": 44,
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          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "NM_001401009.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VDAC1",
          "gene_hgnc_id": 12669,
          "hgvs_c": "c.44G>C",
          "hgvs_p": "p.Arg15Thr",
          "transcript": "NM_001401010.1",
          "protein_id": "NP_001387939.1",
          "transcript_support_level": null,
          "aa_start": 15,
          "aa_end": null,
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          "cds_start": 44,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          "canonical": false,
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          "consequences": [
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          ],
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          "intron_rank": null,
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          "gene_symbol": "VDAC1",
          "gene_hgnc_id": 12669,
          "hgvs_c": "c.44G>C",
          "hgvs_p": "p.Arg15Thr",
          "transcript": "NM_001401011.1",
          "protein_id": "NP_001387940.1",
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          "cds_start": 44,
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          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "NM_001401011.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
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          "intron_rank": null,
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          "gene_symbol": "VDAC1",
          "gene_hgnc_id": 12669,
          "hgvs_c": "c.44G>C",
          "hgvs_p": "p.Arg15Thr",
          "transcript": "NM_001401016.1",
          "protein_id": "NP_001387945.1",
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001401016.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VDAC1",
          "gene_hgnc_id": 12669,
          "hgvs_c": "c.44G>C",
          "hgvs_p": "p.Arg15Thr",
          "transcript": "NM_001401017.1",
          "protein_id": "NP_001387946.1",
          "transcript_support_level": null,
          "aa_start": 15,
          "aa_end": null,
          "aa_length": 283,
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          "cdna_start": null,
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          "cdna_length": null,
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          "biotype": "protein_coding",
          "feature": "NM_001401017.1"
        },
        {
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        {
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          "gene_symbol": "VDAC1",
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          "transcript": "NM_001401029.1",
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        {
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          "gene_symbol": "VDAC1",
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          "hgvs_c": "c.-330G>C",
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          "transcript": "NM_001401031.1",
          "protein_id": "NP_001387960.1",
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          "cds_start": null,
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          "biotype": "protein_coding",
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        },
        {
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          "protein_coding": true,
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            "5_prime_UTR_variant"
          ],
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          "intron_rank": null,
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          "gene_symbol": "VDAC1",
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          "transcript": "NM_001401032.1",
          "protein_id": "NP_001387961.1",
          "transcript_support_level": null,
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          "biotype": "protein_coding",
          "feature": "NM_001401032.1"
        },
        {
          "aa_ref": null,
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          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
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          "exon_count": 6,
          "intron_rank": null,
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          "gene_symbol": "VDAC1",
          "gene_hgnc_id": 12669,
          "hgvs_c": "n.96G>C",
          "hgvs_p": null,
          "transcript": "ENST00000466080.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": null,
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          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000466080.1"
        }
      ],
      "gene_symbol": "VDAC1",
      "gene_hgnc_id": 12669,
      "dbsnp": "rs1753162445",
      "frequency_reference_population": 0.0000013685639,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 2,
      "gnomad_exomes_af": 0.00000136856,
      "gnomad_genomes_af": 0.00000656789,
      "gnomad_exomes_ac": 2,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.7589322328567505,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.348,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.9139,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.08,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 3.231,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 3,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3",
      "acmg_by_gene": [
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
            "PM2",
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001401028.1",
          "gene_symbol": "VDAC1",
          "hgnc_id": 12669,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.44G>C",
          "hgvs_p": "p.Arg15Thr"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}