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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-13762777-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=13762777&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "message": null,
  "variants": [
    {
      "acmg_by_gene": [
        {
          "benign_score": 0,
          "criteria": [
            "PP3_Strong",
            "PP5_Very_Strong"
          ],
          "effects": [
            "missense_variant"
          ],
          "gene_symbol": "DNAH5",
          "hgnc_id": 2950,
          "hgvs_c": "c.10226G>C",
          "hgvs_p": "p.Trp3409Ser",
          "inheritance_mode": "AD,AR",
          "pathogenic_score": 12,
          "score": 12,
          "transcript": "NM_001369.3",
          "verdict": "Pathogenic"
        }
      ],
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PP3_Strong,PP5_Very_Strong",
      "acmg_score": 12,
      "allele_count_reference_population": 25,
      "alphamissense_prediction": "Pathogenic",
      "alphamissense_score": 0.9892,
      "alt": "G",
      "apogee2_prediction": null,
      "apogee2_score": null,
      "bayesdelnoaf_prediction": "Pathogenic",
      "bayesdelnoaf_score": 0.38,
      "chr": "5",
      "clinvar_classification": "Pathogenic/Likely pathogenic",
      "clinvar_disease": "Primary ciliary dyskinesia",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:1 LP:1",
      "computational_prediction_selected": "Pathogenic",
      "computational_score_selected": 0.990557074546814,
      "computational_source_selected": "MetaRNN",
      "consequences": [
        {
          "aa_alt": "S",
          "aa_end": null,
          "aa_length": 4624,
          "aa_ref": "W",
          "aa_start": 3409,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 15781,
          "cdna_start": 10476,
          "cds_end": null,
          "cds_length": 13875,
          "cds_start": 10226,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 79,
          "exon_rank": 60,
          "exon_rank_end": null,
          "feature": "NM_001369.3",
          "gene_hgnc_id": 2950,
          "gene_symbol": "DNAH5",
          "hgvs_c": "c.10226G>C",
          "hgvs_p": "p.Trp3409Ser",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "ENST00000265104.5",
          "protein_coding": true,
          "protein_id": "NP_001360.1",
          "strand": false,
          "transcript": "NM_001369.3",
          "transcript_support_level": null
        },
        {
          "aa_alt": "S",
          "aa_end": null,
          "aa_length": 4624,
          "aa_ref": "W",
          "aa_start": 3409,
          "biotype": "protein_coding",
          "canonical": true,
          "cdna_end": null,
          "cdna_length": 15781,
          "cdna_start": 10476,
          "cds_end": null,
          "cds_length": 13875,
          "cds_start": 10226,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 79,
          "exon_rank": 60,
          "exon_rank_end": null,
          "feature": "ENST00000265104.5",
          "gene_hgnc_id": 2950,
          "gene_symbol": "DNAH5",
          "hgvs_c": "c.10226G>C",
          "hgvs_p": "p.Trp3409Ser",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "NM_001369.3",
          "protein_coding": true,
          "protein_id": "ENSP00000265104.4",
          "strand": false,
          "transcript": "ENST00000265104.5",
          "transcript_support_level": 1
        },
        {
          "aa_alt": "S",
          "aa_end": null,
          "aa_length": 4609,
          "aa_ref": "W",
          "aa_start": 3394,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 15645,
          "cdna_start": 10340,
          "cds_end": null,
          "cds_length": 13830,
          "cds_start": 10181,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 79,
          "exon_rank": 60,
          "exon_rank_end": null,
          "feature": "ENST00000681290.1",
          "gene_hgnc_id": 2950,
          "gene_symbol": "DNAH5",
          "hgvs_c": "c.10181G>C",
          "hgvs_p": "p.Trp3394Ser",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000505288.1",
          "strand": false,
          "transcript": "ENST00000681290.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "S",
          "aa_end": null,
          "aa_length": 4660,
          "aa_ref": "W",
          "aa_start": 3445,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 15645,
          "cdna_start": 10340,
          "cds_end": null,
          "cds_length": 13983,
          "cds_start": 10334,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 79,
          "exon_rank": 60,
          "exon_rank_end": null,
          "feature": "XM_005248262.4",
          "gene_hgnc_id": 2950,
          "gene_symbol": "DNAH5",
          "hgvs_c": "c.10334G>C",
          "hgvs_p": "p.Trp3445Ser",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_005248319.2",
          "strand": false,
          "transcript": "XM_005248262.4",
          "transcript_support_level": null
        },
        {
          "aa_alt": "S",
          "aa_end": null,
          "aa_length": 4544,
          "aa_ref": "W",
          "aa_start": 3329,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 16410,
          "cdna_start": 11105,
          "cds_end": null,
          "cds_length": 13635,
          "cds_start": 9986,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 79,
          "exon_rank": 60,
          "exon_rank_end": null,
          "feature": "XM_047416886.1",
          "gene_hgnc_id": 2950,
          "gene_symbol": "DNAH5",
          "hgvs_c": "c.9986G>C",
          "hgvs_p": "p.Trp3329Ser",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_047272842.1",
          "strand": false,
          "transcript": "XM_047416886.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "S",
          "aa_end": null,
          "aa_length": 4520,
          "aa_ref": "W",
          "aa_start": 3445,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 15225,
          "cdna_start": 10340,
          "cds_end": null,
          "cds_length": 13563,
          "cds_start": 10334,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 77,
          "exon_rank": 60,
          "exon_rank_end": null,
          "feature": "XM_017009177.2",
          "gene_hgnc_id": 2950,
          "gene_symbol": "DNAH5",
          "hgvs_c": "c.10334G>C",
          "hgvs_p": "p.Trp3445Ser",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_016864666.1",
          "strand": false,
          "transcript": "XM_017009177.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": "S",
          "aa_end": null,
          "aa_length": 4295,
          "aa_ref": "W",
          "aa_start": 3080,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 14753,
          "cdna_start": 9448,
          "cds_end": null,
          "cds_length": 12888,
          "cds_start": 9239,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 73,
          "exon_rank": 54,
          "exon_rank_end": null,
          "feature": "XM_017009179.3",
          "gene_hgnc_id": 2950,
          "gene_symbol": "DNAH5",
          "hgvs_c": "c.9239G>C",
          "hgvs_p": "p.Trp3080Ser",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_016864668.1",
          "strand": false,
          "transcript": "XM_017009179.3",
          "transcript_support_level": null
        },
        {
          "aa_alt": "S",
          "aa_end": null,
          "aa_length": 4272,
          "aa_ref": "W",
          "aa_start": 3445,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 12932,
          "cdna_start": 10340,
          "cds_end": null,
          "cds_length": 12819,
          "cds_start": 10334,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 74,
          "exon_rank": 60,
          "exon_rank_end": null,
          "feature": "XM_017009180.2",
          "gene_hgnc_id": 2950,
          "gene_symbol": "DNAH5",
          "hgvs_c": "c.10334G>C",
          "hgvs_p": "p.Trp3445Ser",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_016864669.1",
          "strand": false,
          "transcript": "XM_017009180.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": "S",
          "aa_end": null,
          "aa_length": 3960,
          "aa_ref": "W",
          "aa_start": 3445,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 11987,
          "cdna_start": 10340,
          "cds_end": null,
          "cds_length": 11883,
          "cds_start": 10334,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 69,
          "exon_rank": 60,
          "exon_rank_end": null,
          "feature": "XM_017009181.2",
          "gene_hgnc_id": 2950,
          "gene_symbol": "DNAH5",
          "hgvs_c": "c.10334G>C",
          "hgvs_p": "p.Trp3445Ser",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_016864670.1",
          "strand": false,
          "transcript": "XM_017009181.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": "S",
          "aa_end": null,
          "aa_length": 3809,
          "aa_ref": "W",
          "aa_start": 3445,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 11526,
          "cdna_start": 10340,
          "cds_end": null,
          "cds_length": 11430,
          "cds_start": 10334,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 67,
          "exon_rank": 60,
          "exon_rank_end": null,
          "feature": "XM_017009182.2",
          "gene_hgnc_id": 2950,
          "gene_symbol": "DNAH5",
          "hgvs_c": "c.10334G>C",
          "hgvs_p": "p.Trp3445Ser",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_016864671.1",
          "strand": false,
          "transcript": "XM_017009182.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": "S",
          "aa_end": null,
          "aa_length": 3023,
          "aa_ref": "W",
          "aa_start": 1808,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 10952,
          "cdna_start": 5647,
          "cds_end": null,
          "cds_length": 9072,
          "cds_start": 5423,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 51,
          "exon_rank": 32,
          "exon_rank_end": null,
          "feature": "XM_017009185.1",
          "gene_hgnc_id": 2950,
          "gene_symbol": "DNAH5",
          "hgvs_c": "c.5423G>C",
          "hgvs_p": "p.Trp1808Ser",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_016864674.1",
          "strand": false,
          "transcript": "XM_017009185.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "S",
          "aa_end": null,
          "aa_length": 2874,
          "aa_ref": "W",
          "aa_start": 1659,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 10383,
          "cdna_start": 5078,
          "cds_end": null,
          "cds_length": 8625,
          "cds_start": 4976,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 48,
          "exon_rank": 29,
          "exon_rank_end": null,
          "feature": "XM_017009186.2",
          "gene_hgnc_id": 2950,
          "gene_symbol": "DNAH5",
          "hgvs_c": "c.4976G>C",
          "hgvs_p": "p.Trp1659Ser",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_016864675.1",
          "strand": false,
          "transcript": "XM_017009186.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": "S",
          "aa_end": null,
          "aa_length": 2653,
          "aa_ref": "W",
          "aa_start": 1438,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 11185,
          "cdna_start": 5880,
          "cds_end": null,
          "cds_length": 7962,
          "cds_start": 4313,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 45,
          "exon_rank": 26,
          "exon_rank_end": null,
          "feature": "XM_017009188.2",
          "gene_hgnc_id": 2950,
          "gene_symbol": "DNAH5",
          "hgvs_c": "c.4313G>C",
          "hgvs_p": "p.Trp1438Ser",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_016864677.1",
          "strand": false,
          "transcript": "XM_017009188.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 760,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_count": 5,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "ENST00000504001.3",
          "gene_hgnc_id": 2950,
          "gene_symbol": "DNAH5",
          "hgvs_c": "n.734G>C",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "ENST00000504001.3",
          "transcript_support_level": 5
        }
      ],
      "custom_annotations": null,
      "dbscsnv_ada_prediction": null,
      "dbscsnv_ada_score": null,
      "dbsnp": "rs755407407",
      "effect": "missense_variant",
      "frequency_reference_population": 0.000015489006,
      "gene_hgnc_id": 2950,
      "gene_symbol": "DNAH5",
      "gnomad_exomes_ac": 24,
      "gnomad_exomes_af": 0.0000164177,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_ac": 1,
      "gnomad_genomes_af": 0.00000656996,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_heteroplasmic": null,
      "gnomad_mito_homoplasmic": null,
      "hom_count_reference_population": 0,
      "mitotip_prediction": null,
      "mitotip_score": null,
      "pathogenicity_classification_combined": "Pathogenic/Likely pathogenic",
      "phenotype_combined": "Primary ciliary dyskinesia",
      "phylop100way_prediction": "Pathogenic",
      "phylop100way_score": 7.858,
      "pos": 13762777,
      "ref": "C",
      "revel_prediction": "Pathogenic",
      "revel_score": 0.854,
      "splice_prediction_selected": "Benign",
      "splice_score_selected": 0,
      "splice_source_selected": "max_spliceai",
      "spliceai_max_prediction": "Benign",
      "spliceai_max_score": 0,
      "transcript": "NM_001369.3"
    }
  ]
}
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