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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-141641509-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=141641509&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 141641509,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_033449.3",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCHSD1",
          "gene_hgnc_id": 25463,
          "hgvs_c": "c.2062C>G",
          "hgvs_p": "p.Pro688Ala",
          "transcript": "NM_033449.3",
          "protein_id": "NP_258260.1",
          "transcript_support_level": null,
          "aa_start": 688,
          "aa_end": null,
          "aa_length": 690,
          "cds_start": 2062,
          "cds_end": null,
          "cds_length": 2073,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000435817.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_033449.3"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCHSD1",
          "gene_hgnc_id": 25463,
          "hgvs_c": "c.2062C>G",
          "hgvs_p": "p.Pro688Ala",
          "transcript": "ENST00000435817.7",
          "protein_id": "ENSP00000399259.2",
          "transcript_support_level": 1,
          "aa_start": 688,
          "aa_end": null,
          "aa_length": 690,
          "cds_start": 2062,
          "cds_end": null,
          "cds_length": 2073,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_033449.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000435817.7"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCHSD1",
          "gene_hgnc_id": 25463,
          "hgvs_c": "c.2170C>G",
          "hgvs_p": "p.Pro724Ala",
          "transcript": "ENST00000896539.1",
          "protein_id": "ENSP00000566598.1",
          "transcript_support_level": null,
          "aa_start": 724,
          "aa_end": null,
          "aa_length": 726,
          "cds_start": 2170,
          "cds_end": null,
          "cds_length": 2181,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000896539.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCHSD1",
          "gene_hgnc_id": 25463,
          "hgvs_c": "c.2056C>G",
          "hgvs_p": "p.Pro686Ala",
          "transcript": "ENST00000896537.1",
          "protein_id": "ENSP00000566596.1",
          "transcript_support_level": null,
          "aa_start": 686,
          "aa_end": null,
          "aa_length": 688,
          "cds_start": 2056,
          "cds_end": null,
          "cds_length": 2067,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000896537.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCHSD1",
          "gene_hgnc_id": 25463,
          "hgvs_c": "c.1927C>G",
          "hgvs_p": "p.Pro643Ala",
          "transcript": "ENST00000896538.1",
          "protein_id": "ENSP00000566597.1",
          "transcript_support_level": null,
          "aa_start": 643,
          "aa_end": null,
          "aa_length": 645,
          "cds_start": 1927,
          "cds_end": null,
          "cds_length": 1938,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000896538.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCHSD1",
          "gene_hgnc_id": 25463,
          "hgvs_c": "c.1894C>G",
          "hgvs_p": "p.Pro632Ala",
          "transcript": "ENST00000969298.1",
          "protein_id": "ENSP00000639357.1",
          "transcript_support_level": null,
          "aa_start": 632,
          "aa_end": null,
          "aa_length": 634,
          "cds_start": 1894,
          "cds_end": null,
          "cds_length": 1905,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000969298.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCHSD1",
          "gene_hgnc_id": 25463,
          "hgvs_c": "c.1840C>G",
          "hgvs_p": "p.Pro614Ala",
          "transcript": "ENST00000969297.1",
          "protein_id": "ENSP00000639356.1",
          "transcript_support_level": null,
          "aa_start": 614,
          "aa_end": null,
          "aa_length": 616,
          "cds_start": 1840,
          "cds_end": null,
          "cds_length": 1851,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000969297.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCHSD1",
          "gene_hgnc_id": 25463,
          "hgvs_c": "c.2056C>G",
          "hgvs_p": "p.Pro686Ala",
          "transcript": "XM_005268524.6",
          "protein_id": "XP_005268581.1",
          "transcript_support_level": null,
          "aa_start": 686,
          "aa_end": null,
          "aa_length": 688,
          "cds_start": 2056,
          "cds_end": null,
          "cds_length": 2067,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005268524.6"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCHSD1",
          "gene_hgnc_id": 25463,
          "hgvs_c": "c.1933C>G",
          "hgvs_p": "p.Pro645Ala",
          "transcript": "XM_006714803.5",
          "protein_id": "XP_006714866.1",
          "transcript_support_level": null,
          "aa_start": 645,
          "aa_end": null,
          "aa_length": 647,
          "cds_start": 1933,
          "cds_end": null,
          "cds_length": 1944,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_006714803.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCHSD1",
          "gene_hgnc_id": 25463,
          "hgvs_c": "c.1927C>G",
          "hgvs_p": "p.Pro643Ala",
          "transcript": "XM_047417860.1",
          "protein_id": "XP_047273816.1",
          "transcript_support_level": null,
          "aa_start": 643,
          "aa_end": null,
          "aa_length": 645,
          "cds_start": 1927,
          "cds_end": null,
          "cds_length": 1938,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047417860.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCHSD1",
          "gene_hgnc_id": 25463,
          "hgvs_c": "c.*150C>G",
          "hgvs_p": null,
          "transcript": "ENST00000522783.5",
          "protein_id": "ENSP00000428677.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 598,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1797,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000522783.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCHSD1",
          "gene_hgnc_id": 25463,
          "hgvs_c": "n.419C>G",
          "hgvs_p": null,
          "transcript": "ENST00000518160.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000518160.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCHSD1",
          "gene_hgnc_id": 25463,
          "hgvs_c": "n.975C>G",
          "hgvs_p": null,
          "transcript": "ENST00000520747.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000520747.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCHSD1",
          "gene_hgnc_id": 25463,
          "hgvs_c": "n.*619C>G",
          "hgvs_p": null,
          "transcript": "ENST00000522126.5",
          "protein_id": "ENSP00000427796.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000522126.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCHSD1",
          "gene_hgnc_id": 25463,
          "hgvs_c": "n.1731C>G",
          "hgvs_p": null,
          "transcript": "ENST00000523856.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000523856.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCHSD1",
          "gene_hgnc_id": 25463,
          "hgvs_c": "n.*619C>G",
          "hgvs_p": null,
          "transcript": "ENST00000522126.5",
          "protein_id": "ENSP00000427796.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000522126.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCHSD1",
          "gene_hgnc_id": 25463,
          "hgvs_c": "c.*213C>G",
          "hgvs_p": null,
          "transcript": "XM_047417861.1",
          "protein_id": "XP_047273817.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 632,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1899,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047417861.1"
        }
      ],
      "gene_symbol": "FCHSD1",
      "gene_hgnc_id": 25463,
      "dbsnp": "rs560798832",
      "frequency_reference_population": 7.405191e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 7.40519e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.04257383942604065,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.049,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0603,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.51,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.311,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_033449.3",
          "gene_symbol": "FCHSD1",
          "hgnc_id": 25463,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.2062C>G",
          "hgvs_p": "p.Pro688Ala"
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}
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