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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-146590066-T-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=146590066&ref=T&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 146590066,
      "ref": "T",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_181674.3",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R2B",
          "gene_hgnc_id": 9305,
          "hgvs_c": "c.1213A>C",
          "hgvs_p": "p.Ser405Arg",
          "transcript": "NM_181675.4",
          "protein_id": "NP_858061.3",
          "transcript_support_level": null,
          "aa_start": 405,
          "aa_end": null,
          "aa_length": 443,
          "cds_start": 1213,
          "cds_end": null,
          "cds_length": 1332,
          "cdna_start": 1504,
          "cdna_end": null,
          "cdna_length": 10828,
          "mane_select": "ENST00000394411.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_181675.4"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R2B",
          "gene_hgnc_id": 9305,
          "hgvs_c": "c.1213A>C",
          "hgvs_p": "p.Ser405Arg",
          "transcript": "ENST00000394411.9",
          "protein_id": "ENSP00000377933.3",
          "transcript_support_level": 2,
          "aa_start": 405,
          "aa_end": null,
          "aa_length": 443,
          "cds_start": 1213,
          "cds_end": null,
          "cds_length": 1332,
          "cdna_start": 1504,
          "cdna_end": null,
          "cdna_length": 10828,
          "mane_select": "NM_181675.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000394411.9"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R2B",
          "gene_hgnc_id": 9305,
          "hgvs_c": "c.1411A>C",
          "hgvs_p": "p.Ser471Arg",
          "transcript": "ENST00000394414.5",
          "protein_id": "ENSP00000377936.1",
          "transcript_support_level": 1,
          "aa_start": 471,
          "aa_end": null,
          "aa_length": 509,
          "cds_start": 1411,
          "cds_end": null,
          "cds_length": 1530,
          "cdna_start": 1700,
          "cdna_end": null,
          "cdna_length": 2262,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000394414.5"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R2B",
          "gene_hgnc_id": 9305,
          "hgvs_c": "c.1213A>C",
          "hgvs_p": "p.Ser405Arg",
          "transcript": "ENST00000394409.7",
          "protein_id": "ENSP00000377931.4",
          "transcript_support_level": 1,
          "aa_start": 405,
          "aa_end": null,
          "aa_length": 443,
          "cds_start": 1213,
          "cds_end": null,
          "cds_length": 1332,
          "cdna_start": 1784,
          "cdna_end": null,
          "cdna_length": 10704,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000394409.7"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R2B",
          "gene_hgnc_id": 9305,
          "hgvs_c": "c.1180A>C",
          "hgvs_p": "p.Ser394Arg",
          "transcript": "ENST00000453001.5",
          "protein_id": "ENSP00000398779.2",
          "transcript_support_level": 1,
          "aa_start": 394,
          "aa_end": null,
          "aa_length": 432,
          "cds_start": 1180,
          "cds_end": null,
          "cds_length": 1299,
          "cdna_start": 2012,
          "cdna_end": null,
          "cdna_length": 2569,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000453001.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R2B",
          "gene_hgnc_id": 9305,
          "hgvs_c": "n.*1163A>C",
          "hgvs_p": null,
          "transcript": "ENST00000512639.5",
          "protein_id": "ENSP00000426599.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1927,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000512639.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R2B",
          "gene_hgnc_id": 9305,
          "hgvs_c": "n.1531A>C",
          "hgvs_p": null,
          "transcript": "ENST00000530902.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2093,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000530902.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R2B",
          "gene_hgnc_id": 9305,
          "hgvs_c": "n.*1163A>C",
          "hgvs_p": null,
          "transcript": "ENST00000512639.5",
          "protein_id": "ENSP00000426599.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1927,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000512639.5"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R2B",
          "gene_hgnc_id": 9305,
          "hgvs_c": "c.1411A>C",
          "hgvs_p": "p.Ser471Arg",
          "transcript": "NM_181674.3",
          "protein_id": "NP_858060.2",
          "transcript_support_level": null,
          "aa_start": 471,
          "aa_end": null,
          "aa_length": 509,
          "cds_start": 1411,
          "cds_end": null,
          "cds_length": 1530,
          "cdna_start": 1634,
          "cdna_end": null,
          "cdna_length": 10958,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_181674.3"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R2B",
          "gene_hgnc_id": 9305,
          "hgvs_c": "c.1387A>C",
          "hgvs_p": "p.Ser463Arg",
          "transcript": "NM_001271900.2",
          "protein_id": "NP_001258829.1",
          "transcript_support_level": null,
          "aa_start": 463,
          "aa_end": null,
          "aa_length": 501,
          "cds_start": 1387,
          "cds_end": null,
          "cds_length": 1506,
          "cdna_start": 1593,
          "cdna_end": null,
          "cdna_length": 10917,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001271900.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R2B",
          "gene_hgnc_id": 9305,
          "hgvs_c": "c.1387A>C",
          "hgvs_p": "p.Ser463Arg",
          "transcript": "ENST00000394413.7",
          "protein_id": "ENSP00000377935.4",
          "transcript_support_level": 2,
          "aa_start": 463,
          "aa_end": null,
          "aa_length": 501,
          "cds_start": 1387,
          "cds_end": null,
          "cds_length": 1506,
          "cdna_start": 1588,
          "cdna_end": null,
          "cdna_length": 1982,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000394413.7"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R2B",
          "gene_hgnc_id": 9305,
          "hgvs_c": "c.1231A>C",
          "hgvs_p": "p.Ser411Arg",
          "transcript": "NM_001271899.1",
          "protein_id": "NP_001258828.1",
          "transcript_support_level": null,
          "aa_start": 411,
          "aa_end": null,
          "aa_length": 449,
          "cds_start": 1231,
          "cds_end": null,
          "cds_length": 1350,
          "cdna_start": 1485,
          "cdna_end": null,
          "cdna_length": 2047,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001271899.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R2B",
          "gene_hgnc_id": 9305,
          "hgvs_c": "c.1231A>C",
          "hgvs_p": "p.Ser411Arg",
          "transcript": "ENST00000504198.5",
          "protein_id": "ENSP00000421396.1",
          "transcript_support_level": 2,
          "aa_start": 411,
          "aa_end": null,
          "aa_length": 449,
          "cds_start": 1231,
          "cds_end": null,
          "cds_length": 1350,
          "cdna_start": 1456,
          "cdna_end": null,
          "cdna_length": 2011,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000504198.5"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R2B",
          "gene_hgnc_id": 9305,
          "hgvs_c": "c.1222A>C",
          "hgvs_p": "p.Ser408Arg",
          "transcript": "NM_181676.3",
          "protein_id": "NP_858062.1",
          "transcript_support_level": null,
          "aa_start": 408,
          "aa_end": null,
          "aa_length": 446,
          "cds_start": 1222,
          "cds_end": null,
          "cds_length": 1341,
          "cdna_start": 1440,
          "cdna_end": null,
          "cdna_length": 10764,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_181676.3"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R2B",
          "gene_hgnc_id": 9305,
          "hgvs_c": "c.1222A>C",
          "hgvs_p": "p.Ser408Arg",
          "transcript": "ENST00000336640.10",
          "protein_id": "ENSP00000336591.6",
          "transcript_support_level": 5,
          "aa_start": 408,
          "aa_end": null,
          "aa_length": 446,
          "cds_start": 1222,
          "cds_end": null,
          "cds_length": 1341,
          "cdna_start": 1453,
          "cdna_end": null,
          "cdna_length": 2008,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000336640.10"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R2B",
          "gene_hgnc_id": 9305,
          "hgvs_c": "c.1213A>C",
          "hgvs_p": "p.Ser405Arg",
          "transcript": "NM_001428277.1",
          "protein_id": "NP_001415206.1",
          "transcript_support_level": null,
          "aa_start": 405,
          "aa_end": null,
          "aa_length": 443,
          "cds_start": 1213,
          "cds_end": null,
          "cds_length": 1332,
          "cdna_start": 1899,
          "cdna_end": null,
          "cdna_length": 11223,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001428277.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R2B",
          "gene_hgnc_id": 9305,
          "hgvs_c": "c.1213A>C",
          "hgvs_p": "p.Ser405Arg",
          "transcript": "NM_001428279.1",
          "protein_id": "NP_001415208.1",
          "transcript_support_level": null,
          "aa_start": 405,
          "aa_end": null,
          "aa_length": 443,
          "cds_start": 1213,
          "cds_end": null,
          "cds_length": 1332,
          "cdna_start": 1394,
          "cdna_end": null,
          "cdna_length": 10718,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001428279.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R2B",
          "gene_hgnc_id": 9305,
          "hgvs_c": "c.1213A>C",
          "hgvs_p": "p.Ser405Arg",
          "transcript": "ENST00000885029.1",
          "protein_id": "ENSP00000555088.1",
          "transcript_support_level": null,
          "aa_start": 405,
          "aa_end": null,
          "aa_length": 443,
          "cds_start": 1213,
          "cds_end": null,
          "cds_length": 1332,
          "cdna_start": 1766,
          "cdna_end": null,
          "cdna_length": 2328,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000885029.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R2B",
          "gene_hgnc_id": 9305,
          "hgvs_c": "c.1213A>C",
          "hgvs_p": "p.Ser405Arg",
          "transcript": "ENST00000885030.1",
          "protein_id": "ENSP00000555089.1",
          "transcript_support_level": null,
          "aa_start": 405,
          "aa_end": null,
          "aa_length": 443,
          "cds_start": 1213,
          "cds_end": null,
          "cds_length": 1332,
          "cdna_start": 1636,
          "cdna_end": null,
          "cdna_length": 2189,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000885030.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R2B",
          "gene_hgnc_id": 9305,
          "hgvs_c": "c.1213A>C",
          "hgvs_p": "p.Ser405Arg",
          "transcript": "ENST00000885031.1",
          "protein_id": "ENSP00000555090.1",
          "transcript_support_level": null,
          "aa_start": 405,
          "aa_end": null,
          "aa_length": 443,
          "cds_start": 1213,
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      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.336,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.995,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.12,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 7.674,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BS2",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_181674.3",
          "gene_symbol": "PPP2R2B",
          "hgnc_id": 9305,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.1411A>C",
          "hgvs_p": "p.Ser471Arg"
        },
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000807162.1",
          "gene_symbol": "ENSG00000304935",
          "hgnc_id": null,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.882T>G",
          "hgvs_p": null
        },
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000512730.2",
          "gene_symbol": "ENSG00000250407",
          "hgnc_id": 58421,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.247-16903T>G",
          "hgvs_p": null
        },
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NR_188288.1",
          "gene_symbol": "PPP2R2B-AS2",
          "hgnc_id": 58421,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.247-16903T>G",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not provided,not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:2",
      "phenotype_combined": "not provided|not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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