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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-14713644-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=14713644&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 14713644,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_054027.6",
      "consequences": [
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKH",
          "gene_hgnc_id": 15492,
          "hgvs_c": "c.1165G>A",
          "hgvs_p": "p.Gly389Arg",
          "transcript": "NM_054027.6",
          "protein_id": "NP_473368.1",
          "transcript_support_level": null,
          "aa_start": 389,
          "aa_end": null,
          "aa_length": 492,
          "cds_start": 1165,
          "cds_end": null,
          "cds_length": 1479,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000284268.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_054027.6"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKH",
          "gene_hgnc_id": 15492,
          "hgvs_c": "c.1165G>A",
          "hgvs_p": "p.Gly389Arg",
          "transcript": "ENST00000284268.8",
          "protein_id": "ENSP00000284268.6",
          "transcript_support_level": 1,
          "aa_start": 389,
          "aa_end": null,
          "aa_length": 492,
          "cds_start": 1165,
          "cds_end": null,
          "cds_length": 1479,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_054027.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000284268.8"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKH",
          "gene_hgnc_id": 15492,
          "hgvs_c": "c.1165G>A",
          "hgvs_p": "p.Gly389Arg",
          "transcript": "ENST00000887636.1",
          "protein_id": "ENSP00000557695.1",
          "transcript_support_level": null,
          "aa_start": 389,
          "aa_end": null,
          "aa_length": 490,
          "cds_start": 1165,
          "cds_end": null,
          "cds_length": 1473,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000887636.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKH",
          "gene_hgnc_id": 15492,
          "hgvs_c": "c.1159G>A",
          "hgvs_p": "p.Gly387Arg",
          "transcript": "ENST00000964374.1",
          "protein_id": "ENSP00000634433.1",
          "transcript_support_level": null,
          "aa_start": 387,
          "aa_end": null,
          "aa_length": 490,
          "cds_start": 1159,
          "cds_end": null,
          "cds_length": 1473,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000964374.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKH",
          "gene_hgnc_id": 15492,
          "hgvs_c": "c.1165G>A",
          "hgvs_p": "p.Gly389Arg",
          "transcript": "ENST00000887638.1",
          "protein_id": "ENSP00000557697.1",
          "transcript_support_level": null,
          "aa_start": 389,
          "aa_end": null,
          "aa_length": 489,
          "cds_start": 1165,
          "cds_end": null,
          "cds_length": 1470,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000887638.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKH",
          "gene_hgnc_id": 15492,
          "hgvs_c": "c.1030G>A",
          "hgvs_p": "p.Gly344Arg",
          "transcript": "ENST00000887639.1",
          "protein_id": "ENSP00000557698.1",
          "transcript_support_level": null,
          "aa_start": 344,
          "aa_end": null,
          "aa_length": 447,
          "cds_start": 1030,
          "cds_end": null,
          "cds_length": 1344,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000887639.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKH",
          "gene_hgnc_id": 15492,
          "hgvs_c": "c.859G>A",
          "hgvs_p": "p.Gly287Arg",
          "transcript": "ENST00000887637.1",
          "protein_id": "ENSP00000557696.1",
          "transcript_support_level": null,
          "aa_start": 287,
          "aa_end": null,
          "aa_length": 390,
          "cds_start": 859,
          "cds_end": null,
          "cds_length": 1173,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000887637.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKH",
          "gene_hgnc_id": 15492,
          "hgvs_c": "c.1081G>A",
          "hgvs_p": "p.Gly361Arg",
          "transcript": "XM_017009644.3",
          "protein_id": "XP_016865133.1",
          "transcript_support_level": null,
          "aa_start": 361,
          "aa_end": null,
          "aa_length": 464,
          "cds_start": 1081,
          "cds_end": null,
          "cds_length": 1395,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017009644.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OTULIN",
          "gene_hgnc_id": 25118,
          "hgvs_c": "c.*969C>T",
          "hgvs_p": null,
          "transcript": "ENST00000850613.1",
          "protein_id": "ENSP00000520900.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 352,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1059,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000850613.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OTULIN",
          "gene_hgnc_id": 25118,
          "hgvs_c": "c.*969C>T",
          "hgvs_p": null,
          "transcript": "ENST00000921417.1",
          "protein_id": "ENSP00000591476.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 310,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 933,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000921417.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OTULIN",
          "gene_hgnc_id": 25118,
          "hgvs_c": "c.*969C>T",
          "hgvs_p": null,
          "transcript": "XM_011514151.3",
          "protein_id": "XP_011512453.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 352,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1059,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011514151.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKH",
          "gene_hgnc_id": 15492,
          "hgvs_c": "n.407G>A",
          "hgvs_p": null,
          "transcript": "ENST00000502585.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000502585.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LOC100130744",
          "gene_hgnc_id": null,
          "hgvs_c": "n.951C>T",
          "hgvs_p": null,
          "transcript": "NR_046285.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_046285.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OTULIN",
          "gene_hgnc_id": 25118,
          "hgvs_c": "n.2235C>T",
          "hgvs_p": null,
          "transcript": "XR_007058658.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "XR_007058658.1"
        }
      ],
      "gene_symbol": "ANKH",
      "gene_hgnc_id": 15492,
      "dbsnp": "rs28939080",
      "frequency_reference_population": 6.8408434e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84084e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9086387157440186,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.23000000417232513,
      "splice_prediction_selected": "Uncertain_significance",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.864,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9998,
      "alphamissense_prediction": "Pathogenic",
      "bayesdelnoaf_score": 0.47,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.902,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.23,
      "spliceai_max_prediction": "Uncertain_significance",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 5,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3_Moderate,PP5",
      "acmg_by_gene": [
        {
          "score": 5,
          "benign_score": 0,
          "pathogenic_score": 5,
          "criteria": [
            "PM2",
            "PP3_Moderate",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_054027.6",
          "gene_symbol": "ANKH",
          "hgnc_id": 15492,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.1165G>A",
          "hgvs_p": "p.Gly389Arg"
        },
        {
          "score": 5,
          "benign_score": 0,
          "pathogenic_score": 5,
          "criteria": [
            "PM2",
            "PP3_Moderate",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000850613.1",
          "gene_symbol": "OTULIN",
          "hgnc_id": 25118,
          "effects": [
            "3_prime_UTR_variant"
          ],
          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.*969C>T",
          "hgvs_p": null
        },
        {
          "score": 5,
          "benign_score": 0,
          "pathogenic_score": 5,
          "criteria": [
            "PM2",
            "PP3_Moderate",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NR_046285.1",
          "gene_symbol": "LOC100130744",
          "hgnc_id": null,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.951C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": " autosomal dominant,Chondrocalcinosis 2,Craniometaphyseal dysplasia",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "Craniometaphyseal dysplasia, autosomal dominant|Chondrocalcinosis 2",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}